Isolation and purification of bromelain from waste peel of pineapple for therapeutic application

Detalhes bibliográficos
Autor(a) principal: Bresolin,Iara Rocha Antunes Pereira
Data de Publicação: 2013
Outros Autores: Bresolin,Igor Tadeu Lazzarotto, Silveira,Edgar, Tambourgi,Elias Basile, Mazzola,Priscila Gava
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600012
Resumo: The aim of this work was to isolate and purify bromelain extracted from the pineapple peel by ammonium sulfate precipitation (40-80%), followed by desalting and freeze-drying with a 75% activity recovery and 2.2 fold increased specific activity. Ion exchange chromatography on DEAE-Sepharose was able to separate the polysaccharides from the enzyme, which was recovered in the elution step, maintaining its enzymatic activity. The batch adsorption of bromelain was evaluated in terms of total protein and enzymatic activity using Langmuir and Langmuir-Freundlich models. Results showed that the process could be suitable for the recovery and purification of the enzyme, maintaining its specific activity.
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spelling Isolation and purification of bromelain from waste peel of pineapple for therapeutic applicationBromelainpineapple peeldownstream processingwastetherapeutic applicationsenzymatic activityThe aim of this work was to isolate and purify bromelain extracted from the pineapple peel by ammonium sulfate precipitation (40-80%), followed by desalting and freeze-drying with a 75% activity recovery and 2.2 fold increased specific activity. Ion exchange chromatography on DEAE-Sepharose was able to separate the polysaccharides from the enzyme, which was recovered in the elution step, maintaining its enzymatic activity. The batch adsorption of bromelain was evaluated in terms of total protein and enzymatic activity using Langmuir and Langmuir-Freundlich models. Results showed that the process could be suitable for the recovery and purification of the enzyme, maintaining its specific activity.Instituto de Tecnologia do Paraná - Tecpar2013-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600012Brazilian Archives of Biology and Technology v.56 n.6 2013reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132013000600012info:eu-repo/semantics/openAccessBresolin,Iara Rocha Antunes PereiraBresolin,Igor Tadeu LazzarottoSilveira,EdgarTambourgi,Elias BasileMazzola,Priscila Gavaeng2014-01-15T00:00:00Zoai:scielo:S1516-89132013000600012Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2014-01-15T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Isolation and purification of bromelain from waste peel of pineapple for therapeutic application
title Isolation and purification of bromelain from waste peel of pineapple for therapeutic application
spellingShingle Isolation and purification of bromelain from waste peel of pineapple for therapeutic application
Bresolin,Iara Rocha Antunes Pereira
Bromelain
pineapple peel
downstream processing
waste
therapeutic applications
enzymatic activity
title_short Isolation and purification of bromelain from waste peel of pineapple for therapeutic application
title_full Isolation and purification of bromelain from waste peel of pineapple for therapeutic application
title_fullStr Isolation and purification of bromelain from waste peel of pineapple for therapeutic application
title_full_unstemmed Isolation and purification of bromelain from waste peel of pineapple for therapeutic application
title_sort Isolation and purification of bromelain from waste peel of pineapple for therapeutic application
author Bresolin,Iara Rocha Antunes Pereira
author_facet Bresolin,Iara Rocha Antunes Pereira
Bresolin,Igor Tadeu Lazzarotto
Silveira,Edgar
Tambourgi,Elias Basile
Mazzola,Priscila Gava
author_role author
author2 Bresolin,Igor Tadeu Lazzarotto
Silveira,Edgar
Tambourgi,Elias Basile
Mazzola,Priscila Gava
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Bresolin,Iara Rocha Antunes Pereira
Bresolin,Igor Tadeu Lazzarotto
Silveira,Edgar
Tambourgi,Elias Basile
Mazzola,Priscila Gava
dc.subject.por.fl_str_mv Bromelain
pineapple peel
downstream processing
waste
therapeutic applications
enzymatic activity
topic Bromelain
pineapple peel
downstream processing
waste
therapeutic applications
enzymatic activity
description The aim of this work was to isolate and purify bromelain extracted from the pineapple peel by ammonium sulfate precipitation (40-80%), followed by desalting and freeze-drying with a 75% activity recovery and 2.2 fold increased specific activity. Ion exchange chromatography on DEAE-Sepharose was able to separate the polysaccharides from the enzyme, which was recovered in the elution step, maintaining its enzymatic activity. The batch adsorption of bromelain was evaluated in terms of total protein and enzymatic activity using Langmuir and Langmuir-Freundlich models. Results showed that the process could be suitable for the recovery and purification of the enzyme, maintaining its specific activity.
publishDate 2013
dc.date.none.fl_str_mv 2013-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600012
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600012
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132013000600012
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.56 n.6 2013
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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