Isolation and purification of bromelain from waste peel of pineapple for therapeutic application

Detalhes bibliográficos
Autor(a) principal: Bresolin, Iara Rocha Antunes Pereira [UNIFESP]
Data de Publicação: 2013
Outros Autores: Bresolin, Igor Tadeu Lazzarotto [UNIFESP], Silveira, Edgar, Tambourgi, Elias Basile, Mazzola, Priscila Gava
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/S1516-89132013000600012
http://repositorio.unifesp.br/handle/11600/8026
Resumo: The aim of this work was to isolate and purify bromelain extracted from the pineapple peel by ammonium sulfate precipitation (40-80%), followed by desalting and freeze-drying with a 75% activity recovery and 2.2 fold increased specific activity. Ion exchange chromatography on DEAE-Sepharose was able to separate the polysaccharides from the enzyme, which was recovered in the elution step, maintaining its enzymatic activity. The batch adsorption of bromelain was evaluated in terms of total protein and enzymatic activity using Langmuir and Langmuir-Freundlich models. Results showed that the process could be suitable for the recovery and purification of the enzyme, maintaining its specific activity.
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spelling Isolation and purification of bromelain from waste peel of pineapple for therapeutic applicationBromelainpineapple peeldownstream processingwastetherapeutic applicationsenzymatic activityThe aim of this work was to isolate and purify bromelain extracted from the pineapple peel by ammonium sulfate precipitation (40-80%), followed by desalting and freeze-drying with a 75% activity recovery and 2.2 fold increased specific activity. Ion exchange chromatography on DEAE-Sepharose was able to separate the polysaccharides from the enzyme, which was recovered in the elution step, maintaining its enzymatic activity. The batch adsorption of bromelain was evaluated in terms of total protein and enzymatic activity using Langmuir and Langmuir-Freundlich models. Results showed that the process could be suitable for the recovery and purification of the enzyme, maintaining its specific activity.Universidade Estadual de Campinas Faculdade de Engenharia QuímicaUniversidade Federal de São Paulo (UNIFESP) Departamento de Ciências Exatas e da TerraUniversidade Federal de Uberlândia Instituto de Genética e BioquímicaUniversidade Estadual de Campinas Faculdade de Ciências MédicasUNIFESP, Depto. de Ciências Exatas e da TerraSciELOConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Instituto de Tecnologia do Paraná - TecparUniversidade Estadual de Campinas (UNICAMP)Universidade Federal de São Paulo (UNIFESP)Universidade Federal de Uberlândia Instituto de Genética e BioquímicaBresolin, Iara Rocha Antunes Pereira [UNIFESP]Bresolin, Igor Tadeu Lazzarotto [UNIFESP]Silveira, EdgarTambourgi, Elias BasileMazzola, Priscila Gava2015-06-14T13:45:41Z2015-06-14T13:45:41Z2013-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion971-979application/pdfhttp://dx.doi.org/10.1590/S1516-89132013000600012Brazilian Archives of Biology and Technology. Instituto de Tecnologia do Paraná - Tecpar, v. 56, n. 6, p. 971-979, 2013.10.1590/S1516-89132013000600012S1516-89132013000600012.pdf1516-8913S1516-89132013000600012http://repositorio.unifesp.br/handle/11600/8026WOS:000329451800012engBrazilian Archives of Biology and Technologyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-30T02:01:01Zoai:repositorio.unifesp.br/:11600/8026Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-30T02:01:01Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Isolation and purification of bromelain from waste peel of pineapple for therapeutic application
title Isolation and purification of bromelain from waste peel of pineapple for therapeutic application
spellingShingle Isolation and purification of bromelain from waste peel of pineapple for therapeutic application
Bresolin, Iara Rocha Antunes Pereira [UNIFESP]
Bromelain
pineapple peel
downstream processing
waste
therapeutic applications
enzymatic activity
title_short Isolation and purification of bromelain from waste peel of pineapple for therapeutic application
title_full Isolation and purification of bromelain from waste peel of pineapple for therapeutic application
title_fullStr Isolation and purification of bromelain from waste peel of pineapple for therapeutic application
title_full_unstemmed Isolation and purification of bromelain from waste peel of pineapple for therapeutic application
title_sort Isolation and purification of bromelain from waste peel of pineapple for therapeutic application
author Bresolin, Iara Rocha Antunes Pereira [UNIFESP]
author_facet Bresolin, Iara Rocha Antunes Pereira [UNIFESP]
Bresolin, Igor Tadeu Lazzarotto [UNIFESP]
Silveira, Edgar
Tambourgi, Elias Basile
Mazzola, Priscila Gava
author_role author
author2 Bresolin, Igor Tadeu Lazzarotto [UNIFESP]
Silveira, Edgar
Tambourgi, Elias Basile
Mazzola, Priscila Gava
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Campinas (UNICAMP)
Universidade Federal de São Paulo (UNIFESP)
Universidade Federal de Uberlândia Instituto de Genética e Bioquímica
dc.contributor.author.fl_str_mv Bresolin, Iara Rocha Antunes Pereira [UNIFESP]
Bresolin, Igor Tadeu Lazzarotto [UNIFESP]
Silveira, Edgar
Tambourgi, Elias Basile
Mazzola, Priscila Gava
dc.subject.por.fl_str_mv Bromelain
pineapple peel
downstream processing
waste
therapeutic applications
enzymatic activity
topic Bromelain
pineapple peel
downstream processing
waste
therapeutic applications
enzymatic activity
description The aim of this work was to isolate and purify bromelain extracted from the pineapple peel by ammonium sulfate precipitation (40-80%), followed by desalting and freeze-drying with a 75% activity recovery and 2.2 fold increased specific activity. Ion exchange chromatography on DEAE-Sepharose was able to separate the polysaccharides from the enzyme, which was recovered in the elution step, maintaining its enzymatic activity. The batch adsorption of bromelain was evaluated in terms of total protein and enzymatic activity using Langmuir and Langmuir-Freundlich models. Results showed that the process could be suitable for the recovery and purification of the enzyme, maintaining its specific activity.
publishDate 2013
dc.date.none.fl_str_mv 2013-12-01
2015-06-14T13:45:41Z
2015-06-14T13:45:41Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1516-89132013000600012
Brazilian Archives of Biology and Technology. Instituto de Tecnologia do Paraná - Tecpar, v. 56, n. 6, p. 971-979, 2013.
10.1590/S1516-89132013000600012
S1516-89132013000600012.pdf
1516-8913
S1516-89132013000600012
http://repositorio.unifesp.br/handle/11600/8026
WOS:000329451800012
url http://dx.doi.org/10.1590/S1516-89132013000600012
http://repositorio.unifesp.br/handle/11600/8026
identifier_str_mv Brazilian Archives of Biology and Technology. Instituto de Tecnologia do Paraná - Tecpar, v. 56, n. 6, p. 971-979, 2013.
10.1590/S1516-89132013000600012
S1516-89132013000600012.pdf
1516-8913
S1516-89132013000600012
WOS:000329451800012
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Archives of Biology and Technology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 971-979
application/pdf
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
_version_ 1814268389447696384

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