Nuclear translocation of STAT3 by in vitro metreleptin administration causes lipolysis in human primary adipocytes

Detalhes bibliográficos
Autor(a) principal: Choi,Seung Kug
Data de Publicação: 2016
Outros Autores: Park,Sunmi, Moon,Hyun-Seuk
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100309
Resumo: We utilized subcutaneous (SC)- and omental (OM)-derived human primary adipocytes (hPA) from obese male, and investigated whether synthetic analog of leptin, metreleptin, may regulate lipolysis via translocation of STAT3 to the nucleus. We observed that 50 ng/mL of metreleptin increases STAT3 phosphorylation in both SC- and OM-derived hPA. Importantly, we found for the first time that metreleptin is capable of trans-locating STAT3 to the nucleus and STAT3 blockade inhibits metreleptin-induced lipolysis. Our initial data provide novel insights into the role of STAT3 as probable mediator of the action of metreleptin in regulating metabolism.
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spelling Nuclear translocation of STAT3 by in vitro metreleptin administration causes lipolysis in human primary adipocytesmetreleptinhuman primary adipocyteSTAT3differentiationlipolysisWe utilized subcutaneous (SC)- and omental (OM)-derived human primary adipocytes (hPA) from obese male, and investigated whether synthetic analog of leptin, metreleptin, may regulate lipolysis via translocation of STAT3 to the nucleus. We observed that 50 ng/mL of metreleptin increases STAT3 phosphorylation in both SC- and OM-derived hPA. Importantly, we found for the first time that metreleptin is capable of trans-locating STAT3 to the nucleus and STAT3 blockade inhibits metreleptin-induced lipolysis. Our initial data provide novel insights into the role of STAT3 as probable mediator of the action of metreleptin in regulating metabolism.Instituto de Tecnologia do Paraná - Tecpar2016-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100309Brazilian Archives of Biology and Technology v.59 2016reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/1678-4324-2016150605info:eu-repo/semantics/openAccessChoi,Seung KugPark,SunmiMoon,Hyun-Seukeng2017-01-20T00:00:00Zoai:scielo:S1516-89132016000100309Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2017-01-20T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Nuclear translocation of STAT3 by in vitro metreleptin administration causes lipolysis in human primary adipocytes
title Nuclear translocation of STAT3 by in vitro metreleptin administration causes lipolysis in human primary adipocytes
spellingShingle Nuclear translocation of STAT3 by in vitro metreleptin administration causes lipolysis in human primary adipocytes
Choi,Seung Kug
metreleptin
human primary adipocyte
STAT3
differentiation
lipolysis
title_short Nuclear translocation of STAT3 by in vitro metreleptin administration causes lipolysis in human primary adipocytes
title_full Nuclear translocation of STAT3 by in vitro metreleptin administration causes lipolysis in human primary adipocytes
title_fullStr Nuclear translocation of STAT3 by in vitro metreleptin administration causes lipolysis in human primary adipocytes
title_full_unstemmed Nuclear translocation of STAT3 by in vitro metreleptin administration causes lipolysis in human primary adipocytes
title_sort Nuclear translocation of STAT3 by in vitro metreleptin administration causes lipolysis in human primary adipocytes
author Choi,Seung Kug
author_facet Choi,Seung Kug
Park,Sunmi
Moon,Hyun-Seuk
author_role author
author2 Park,Sunmi
Moon,Hyun-Seuk
author2_role author
author
dc.contributor.author.fl_str_mv Choi,Seung Kug
Park,Sunmi
Moon,Hyun-Seuk
dc.subject.por.fl_str_mv metreleptin
human primary adipocyte
STAT3
differentiation
lipolysis
topic metreleptin
human primary adipocyte
STAT3
differentiation
lipolysis
description We utilized subcutaneous (SC)- and omental (OM)-derived human primary adipocytes (hPA) from obese male, and investigated whether synthetic analog of leptin, metreleptin, may regulate lipolysis via translocation of STAT3 to the nucleus. We observed that 50 ng/mL of metreleptin increases STAT3 phosphorylation in both SC- and OM-derived hPA. Importantly, we found for the first time that metreleptin is capable of trans-locating STAT3 to the nucleus and STAT3 blockade inhibits metreleptin-induced lipolysis. Our initial data provide novel insights into the role of STAT3 as probable mediator of the action of metreleptin in regulating metabolism.
publishDate 2016
dc.date.none.fl_str_mv 2016-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100309
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100309
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1678-4324-2016150605
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.59 2016
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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