Knockdown of CPT1A Induce Chicken Adipocyte Differentiation to Form Lipid Droplets

Detalhes bibliográficos
Autor(a) principal: Li,ZQ
Data de Publicação: 2022
Outros Autores: Li,JJ, Lin,ZZ, Zhang,DH, Zhang,GF, Ran,JS, Wang,Y, Yin,HD, Liu,YP
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Poultry Science (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2022000400503
Resumo: ABSTRACT Lipid metabolism dysfunction is closely related to obesity, inflammation, diabetes, lipodystrophy, cardiovascular disease. Along with having a positive effect on energy homeostasis during fasting or prolonged exercise through mitochondrial fatty acid oxidation (FAO), more than two dozen enzymes and transport proteins are involved in the activation and transport of fatty acids into the mitochondrial, providing insights into their critical roles in metabolism. CPT1A has been reported to be expressed ubiquitously in the body and associated with dire consequences affecting fat deposition as the key rate-limiting enzyme of FAO. However, there is a dearth of data on the specific role of CPT1A on adipogenic differentiation and adipocyte lipolysis on chicken. This study assessed CPT1A’s function in adipocyte differentiation andadipocyte lipolysis, and the mechanisms were investigated. We found that CPT1A knockdown (KD) promotes the differentiation of chicken preadipocytes into mature adipocytes. CPT1A KD increased PPARγ protein expression level. Expression levels of lipid synthesis-related genes were increased, and lipolysis genes were reduced. Also, CPT1A KD can encourage the formation of lipid droplets. So our results confirmed that knockdown of CPT1A induced the lipid differentiation and inhibited the β-oxidation process to promote the formation of lipid droplets. These findings may deepen our understanding on CPT1A function, especially its regulatory role in adipocyte biology.
id FACTA-1_28ce47ea868c31535836a444b56fe0b6
oai_identifier_str oai:scielo:S1516-635X2022000400503
network_acronym_str FACTA-1
network_name_str Brazilian Journal of Poultry Science (Online)
repository_id_str
spelling Knockdown of CPT1A Induce Chicken Adipocyte Differentiation to Form Lipid DropletsAdipocyte differentiationCPT1Alipid dropletsABSTRACT Lipid metabolism dysfunction is closely related to obesity, inflammation, diabetes, lipodystrophy, cardiovascular disease. Along with having a positive effect on energy homeostasis during fasting or prolonged exercise through mitochondrial fatty acid oxidation (FAO), more than two dozen enzymes and transport proteins are involved in the activation and transport of fatty acids into the mitochondrial, providing insights into their critical roles in metabolism. CPT1A has been reported to be expressed ubiquitously in the body and associated with dire consequences affecting fat deposition as the key rate-limiting enzyme of FAO. However, there is a dearth of data on the specific role of CPT1A on adipogenic differentiation and adipocyte lipolysis on chicken. This study assessed CPT1A’s function in adipocyte differentiation andadipocyte lipolysis, and the mechanisms were investigated. We found that CPT1A knockdown (KD) promotes the differentiation of chicken preadipocytes into mature adipocytes. CPT1A KD increased PPARγ protein expression level. Expression levels of lipid synthesis-related genes were increased, and lipolysis genes were reduced. Also, CPT1A KD can encourage the formation of lipid droplets. So our results confirmed that knockdown of CPT1A induced the lipid differentiation and inhibited the β-oxidation process to promote the formation of lipid droplets. These findings may deepen our understanding on CPT1A function, especially its regulatory role in adipocyte biology.Fundacao de Apoio a Ciência e Tecnologia Avicolas2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2022000400503Brazilian Journal of Poultry Science v.24 n.4 2022reponame:Brazilian Journal of Poultry Science (Online)instname:Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)instacron:FACTA10.1590/1806-9061-2021-1589info:eu-repo/semantics/openAccessLi,ZQLi,JJLin,ZZZhang,DHZhang,GFRan,JSWang,YYin,HDLiu,YPeng2022-08-03T00:00:00Zoai:scielo:S1516-635X2022000400503Revistahttp://www.scielo.br/rbcahttps://old.scielo.br/oai/scielo-oai.php||rvfacta@terra.com.br1806-90611516-635Xopendoar:2022-08-03T00:00Brazilian Journal of Poultry Science (Online) - Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)false
dc.title.none.fl_str_mv Knockdown of CPT1A Induce Chicken Adipocyte Differentiation to Form Lipid Droplets
title Knockdown of CPT1A Induce Chicken Adipocyte Differentiation to Form Lipid Droplets
spellingShingle Knockdown of CPT1A Induce Chicken Adipocyte Differentiation to Form Lipid Droplets
Li,ZQ
Adipocyte differentiation
CPT1A
lipid droplets
title_short Knockdown of CPT1A Induce Chicken Adipocyte Differentiation to Form Lipid Droplets
title_full Knockdown of CPT1A Induce Chicken Adipocyte Differentiation to Form Lipid Droplets
title_fullStr Knockdown of CPT1A Induce Chicken Adipocyte Differentiation to Form Lipid Droplets
title_full_unstemmed Knockdown of CPT1A Induce Chicken Adipocyte Differentiation to Form Lipid Droplets
title_sort Knockdown of CPT1A Induce Chicken Adipocyte Differentiation to Form Lipid Droplets
author Li,ZQ
author_facet Li,ZQ
Li,JJ
Lin,ZZ
Zhang,DH
Zhang,GF
Ran,JS
Wang,Y
Yin,HD
Liu,YP
author_role author
author2 Li,JJ
Lin,ZZ
Zhang,DH
Zhang,GF
Ran,JS
Wang,Y
Yin,HD
Liu,YP
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Li,ZQ
Li,JJ
Lin,ZZ
Zhang,DH
Zhang,GF
Ran,JS
Wang,Y
Yin,HD
Liu,YP
dc.subject.por.fl_str_mv Adipocyte differentiation
CPT1A
lipid droplets
topic Adipocyte differentiation
CPT1A
lipid droplets
description ABSTRACT Lipid metabolism dysfunction is closely related to obesity, inflammation, diabetes, lipodystrophy, cardiovascular disease. Along with having a positive effect on energy homeostasis during fasting or prolonged exercise through mitochondrial fatty acid oxidation (FAO), more than two dozen enzymes and transport proteins are involved in the activation and transport of fatty acids into the mitochondrial, providing insights into their critical roles in metabolism. CPT1A has been reported to be expressed ubiquitously in the body and associated with dire consequences affecting fat deposition as the key rate-limiting enzyme of FAO. However, there is a dearth of data on the specific role of CPT1A on adipogenic differentiation and adipocyte lipolysis on chicken. This study assessed CPT1A’s function in adipocyte differentiation andadipocyte lipolysis, and the mechanisms were investigated. We found that CPT1A knockdown (KD) promotes the differentiation of chicken preadipocytes into mature adipocytes. CPT1A KD increased PPARγ protein expression level. Expression levels of lipid synthesis-related genes were increased, and lipolysis genes were reduced. Also, CPT1A KD can encourage the formation of lipid droplets. So our results confirmed that knockdown of CPT1A induced the lipid differentiation and inhibited the β-oxidation process to promote the formation of lipid droplets. These findings may deepen our understanding on CPT1A function, especially its regulatory role in adipocyte biology.
publishDate 2022
dc.date.none.fl_str_mv 2022-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2022000400503
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2022000400503
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1806-9061-2021-1589
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Fundacao de Apoio a Ciência e Tecnologia Avicolas
publisher.none.fl_str_mv Fundacao de Apoio a Ciência e Tecnologia Avicolas
dc.source.none.fl_str_mv Brazilian Journal of Poultry Science v.24 n.4 2022
reponame:Brazilian Journal of Poultry Science (Online)
instname:Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)
instacron:FACTA
instname_str Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)
instacron_str FACTA
institution FACTA
reponame_str Brazilian Journal of Poultry Science (Online)
collection Brazilian Journal of Poultry Science (Online)
repository.name.fl_str_mv Brazilian Journal of Poultry Science (Online) - Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)
repository.mail.fl_str_mv ||rvfacta@terra.com.br
_version_ 1754122516129906688