Semliki forest virus as a vector: pros and cons for its use in biopharmaceuticals production

Detalhes bibliográficos
Autor(a) principal: Núñez,Eutimio Gustavo Fernández
Data de Publicação: 2013
Outros Autores: Jorge,Soraia Attie Calil, Astray,Renato Mancini, Rezende,Alexandre Gonçalves de, Costa,Bruno Labate Vale da, Monteiro,Daniella Cristina Ventini, Pereira,Carlos Augusto, Tonso,Aldo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500018
Resumo: The number of biopharmaceuticals for medical and veterinarian use produced in mammalian cells is increasing year after year. All of them are obtained by stable recombinant cell lines. However, it is recognized that transient gene expression produces high level expression in a short time. In that sense, viral vectors have been extensively used for producing recombinant proteins on lab-scale. Among them, Semliki Forest virus is commonly employed for this purpose. This review discusses the main aspects related to the use of Semliki Forest virus technology as well as its advantages and drawbacks which limit currently its utilization in biopharmaceutical industry on large-scale.
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spelling Semliki forest virus as a vector: pros and cons for its use in biopharmaceuticals productionBHK-21Large-scale bioprocessesMammalian cellsRecombinant proteinsSFVTransient expressionThe number of biopharmaceuticals for medical and veterinarian use produced in mammalian cells is increasing year after year. All of them are obtained by stable recombinant cell lines. However, it is recognized that transient gene expression produces high level expression in a short time. In that sense, viral vectors have been extensively used for producing recombinant proteins on lab-scale. Among them, Semliki Forest virus is commonly employed for this purpose. This review discusses the main aspects related to the use of Semliki Forest virus technology as well as its advantages and drawbacks which limit currently its utilization in biopharmaceutical industry on large-scale.Instituto de Tecnologia do Paraná - Tecpar2013-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500018Brazilian Archives of Biology and Technology v.56 n.5 2013reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132013000500018info:eu-repo/semantics/openAccessNúñez,Eutimio Gustavo FernándezJorge,Soraia Attie CalilAstray,Renato ManciniRezende,Alexandre Gonçalves deCosta,Bruno Labate Vale daMonteiro,Daniella Cristina VentiniPereira,Carlos AugustoTonso,Aldoeng2013-10-31T00:00:00Zoai:scielo:S1516-89132013000500018Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2013-10-31T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Semliki forest virus as a vector: pros and cons for its use in biopharmaceuticals production
title Semliki forest virus as a vector: pros and cons for its use in biopharmaceuticals production
spellingShingle Semliki forest virus as a vector: pros and cons for its use in biopharmaceuticals production
Núñez,Eutimio Gustavo Fernández
BHK-21
Large-scale bioprocesses
Mammalian cells
Recombinant proteins
SFV
Transient expression
title_short Semliki forest virus as a vector: pros and cons for its use in biopharmaceuticals production
title_full Semliki forest virus as a vector: pros and cons for its use in biopharmaceuticals production
title_fullStr Semliki forest virus as a vector: pros and cons for its use in biopharmaceuticals production
title_full_unstemmed Semliki forest virus as a vector: pros and cons for its use in biopharmaceuticals production
title_sort Semliki forest virus as a vector: pros and cons for its use in biopharmaceuticals production
author Núñez,Eutimio Gustavo Fernández
author_facet Núñez,Eutimio Gustavo Fernández
Jorge,Soraia Attie Calil
Astray,Renato Mancini
Rezende,Alexandre Gonçalves de
Costa,Bruno Labate Vale da
Monteiro,Daniella Cristina Ventini
Pereira,Carlos Augusto
Tonso,Aldo
author_role author
author2 Jorge,Soraia Attie Calil
Astray,Renato Mancini
Rezende,Alexandre Gonçalves de
Costa,Bruno Labate Vale da
Monteiro,Daniella Cristina Ventini
Pereira,Carlos Augusto
Tonso,Aldo
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Núñez,Eutimio Gustavo Fernández
Jorge,Soraia Attie Calil
Astray,Renato Mancini
Rezende,Alexandre Gonçalves de
Costa,Bruno Labate Vale da
Monteiro,Daniella Cristina Ventini
Pereira,Carlos Augusto
Tonso,Aldo
dc.subject.por.fl_str_mv BHK-21
Large-scale bioprocesses
Mammalian cells
Recombinant proteins
SFV
Transient expression
topic BHK-21
Large-scale bioprocesses
Mammalian cells
Recombinant proteins
SFV
Transient expression
description The number of biopharmaceuticals for medical and veterinarian use produced in mammalian cells is increasing year after year. All of them are obtained by stable recombinant cell lines. However, it is recognized that transient gene expression produces high level expression in a short time. In that sense, viral vectors have been extensively used for producing recombinant proteins on lab-scale. Among them, Semliki Forest virus is commonly employed for this purpose. This review discusses the main aspects related to the use of Semliki Forest virus technology as well as its advantages and drawbacks which limit currently its utilization in biopharmaceutical industry on large-scale.
publishDate 2013
dc.date.none.fl_str_mv 2013-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500018
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500018
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132013000500018
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.56 n.5 2013
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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