Semliki forest virus as a vector: Pros and cons for its use in biopharmaceuticals production

Detalhes bibliográficos
Autor(a) principal: Núñez, Eutimio Gustavo Fernández [UNESP]
Data de Publicação: 2013
Outros Autores: Jorge, Soraia Attie Calil, Astray, Renato Mancini, de Rezende, Alexandre Gonçalves, da Costa, Bruno Labate Vale, Monteiro, Daniella Cristina Ventini, Pereira, Carlos Augusto, Tonso, Aldo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1516-89132013000500018
http://hdl.handle.net/11449/227294
Resumo: The number of biopharmaceuticals for medical and veterinarian use produced in mammalian cells is increasing year after year. All of them are obtained by stable recombinant cell lines. However, it is recognized that transient gene expression produces high level expression in a short time. In that sense, viral vectors have been extensively used for producing recombinant proteins on lab-scale. Among them, Semliki Forest virus is commonly employed for this purpose. This review discusses the main aspects related to the use of Semliki Forest virus technology as well as its advantages and drawbacks which limit currently its utilization in biopharmaceutical industry on large-scale.
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spelling Semliki forest virus as a vector: Pros and cons for its use in biopharmaceuticals productionBHK-21Large-scale bioprocessesMammalian cellsRecombinant proteinsSFVTransient expressionThe number of biopharmaceuticals for medical and veterinarian use produced in mammalian cells is increasing year after year. All of them are obtained by stable recombinant cell lines. However, it is recognized that transient gene expression produces high level expression in a short time. In that sense, viral vectors have been extensively used for producing recombinant proteins on lab-scale. Among them, Semliki Forest virus is commonly employed for this purpose. This review discusses the main aspects related to the use of Semliki Forest virus technology as well as its advantages and drawbacks which limit currently its utilization in biopharmaceutical industry on large-scale.Laboratório de Células Animais Departamento de Engenharia Química, Escola Politécnica Universidade de São Paulo, São Paulo SPDepartamento de Ciências Biológicas Faculdade de Ciência e Letras Universidade Estadual Paulista Julho de Mesquita Filho, Assis SPLaboratório de Imunologia Viral Instituto Butantan, São Paulo SPLaboratório de Biotecnologia Industrial Núcleo de Bionanomanufatura Instituto de Pesquisas Tecnológicas do Estado de São Paulo, São Paulo SPDepartamento de Ciências Biológicas Faculdade de Ciência e Letras Universidade Estadual Paulista Julho de Mesquita Filho, Assis SPUniversidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)Instituto ButantanInstituto de Pesquisas Tecnológicas do Estado de São PauloNúñez, Eutimio Gustavo Fernández [UNESP]Jorge, Soraia Attie CalilAstray, Renato Mancinide Rezende, Alexandre Gonçalvesda Costa, Bruno Labate ValeMonteiro, Daniella Cristina VentiniPereira, Carlos AugustoTonso, Aldo2022-04-29T07:12:26Z2022-04-29T07:12:26Z2013-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article859-866http://dx.doi.org/10.1590/S1516-89132013000500018Brazilian Archives of Biology and Technology, v. 56, n. 5, p. 859-866, 2013.1516-89131678-4324http://hdl.handle.net/11449/22729410.1590/S1516-891320130005000182-s2.0-84887180324Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Archives of Biology and Technologyinfo:eu-repo/semantics/openAccess2022-04-29T07:12:26Zoai:repositorio.unesp.br:11449/227294Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-29T07:12:26Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Semliki forest virus as a vector: Pros and cons for its use in biopharmaceuticals production
title Semliki forest virus as a vector: Pros and cons for its use in biopharmaceuticals production
spellingShingle Semliki forest virus as a vector: Pros and cons for its use in biopharmaceuticals production
Núñez, Eutimio Gustavo Fernández [UNESP]
BHK-21
Large-scale bioprocesses
Mammalian cells
Recombinant proteins
SFV
Transient expression
title_short Semliki forest virus as a vector: Pros and cons for its use in biopharmaceuticals production
title_full Semliki forest virus as a vector: Pros and cons for its use in biopharmaceuticals production
title_fullStr Semliki forest virus as a vector: Pros and cons for its use in biopharmaceuticals production
title_full_unstemmed Semliki forest virus as a vector: Pros and cons for its use in biopharmaceuticals production
title_sort Semliki forest virus as a vector: Pros and cons for its use in biopharmaceuticals production
author Núñez, Eutimio Gustavo Fernández [UNESP]
author_facet Núñez, Eutimio Gustavo Fernández [UNESP]
Jorge, Soraia Attie Calil
Astray, Renato Mancini
de Rezende, Alexandre Gonçalves
da Costa, Bruno Labate Vale
Monteiro, Daniella Cristina Ventini
Pereira, Carlos Augusto
Tonso, Aldo
author_role author
author2 Jorge, Soraia Attie Calil
Astray, Renato Mancini
de Rezende, Alexandre Gonçalves
da Costa, Bruno Labate Vale
Monteiro, Daniella Cristina Ventini
Pereira, Carlos Augusto
Tonso, Aldo
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Estadual Paulista (UNESP)
Instituto Butantan
Instituto de Pesquisas Tecnológicas do Estado de São Paulo
dc.contributor.author.fl_str_mv Núñez, Eutimio Gustavo Fernández [UNESP]
Jorge, Soraia Attie Calil
Astray, Renato Mancini
de Rezende, Alexandre Gonçalves
da Costa, Bruno Labate Vale
Monteiro, Daniella Cristina Ventini
Pereira, Carlos Augusto
Tonso, Aldo
dc.subject.por.fl_str_mv BHK-21
Large-scale bioprocesses
Mammalian cells
Recombinant proteins
SFV
Transient expression
topic BHK-21
Large-scale bioprocesses
Mammalian cells
Recombinant proteins
SFV
Transient expression
description The number of biopharmaceuticals for medical and veterinarian use produced in mammalian cells is increasing year after year. All of them are obtained by stable recombinant cell lines. However, it is recognized that transient gene expression produces high level expression in a short time. In that sense, viral vectors have been extensively used for producing recombinant proteins on lab-scale. Among them, Semliki Forest virus is commonly employed for this purpose. This review discusses the main aspects related to the use of Semliki Forest virus technology as well as its advantages and drawbacks which limit currently its utilization in biopharmaceutical industry on large-scale.
publishDate 2013
dc.date.none.fl_str_mv 2013-09-01
2022-04-29T07:12:26Z
2022-04-29T07:12:26Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1516-89132013000500018
Brazilian Archives of Biology and Technology, v. 56, n. 5, p. 859-866, 2013.
1516-8913
1678-4324
http://hdl.handle.net/11449/227294
10.1590/S1516-89132013000500018
2-s2.0-84887180324
url http://dx.doi.org/10.1590/S1516-89132013000500018
http://hdl.handle.net/11449/227294
identifier_str_mv Brazilian Archives of Biology and Technology, v. 56, n. 5, p. 859-866, 2013.
1516-8913
1678-4324
10.1590/S1516-89132013000500018
2-s2.0-84887180324
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Archives of Biology and Technology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 859-866
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1799965281225801728

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