Spectrophotometric determination of tannins and caffeine in preparations from Paullinia cupana var. sorbilis

Detalhes bibliográficos
Autor(a) principal: Pelozo,Maria Inez de Godoy
Data de Publicação: 2008
Outros Autores: Cardoso,Mara Lane Carvalho, Mello,João Carlos Palazzo de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300002
Resumo: The present work was designed to quantify the caffeine and total polyphenols in the extractive solution and the granulated form from the seeds of Paullinia cupana var. sorbilis, by the spectrophotometric method. The method showed linearity for the caffeine and polyphenols in the range of 5-25 µg/ml and 2.4-5.6 µg/ml respectively. The solutions of the semipurified fraction (EPA) and granulated form (GRA) showed linear responses in the range of 0.288-0.672 and 0.4-1.2 µg/ml, respectively. The precision and accuracy were determined for the EPA solution at a concentration of 100 µg/ml. The spectrophotometric method performed well in quantifying the caffeine and total polyphenols.
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spelling Spectrophotometric determination of tannins and caffeine in preparations from Paullinia cupana var. sorbilisCaffeinePaullinia cupanaTotal polyphenolsSpectrophotometric quantification assayThe present work was designed to quantify the caffeine and total polyphenols in the extractive solution and the granulated form from the seeds of Paullinia cupana var. sorbilis, by the spectrophotometric method. The method showed linearity for the caffeine and polyphenols in the range of 5-25 µg/ml and 2.4-5.6 µg/ml respectively. The solutions of the semipurified fraction (EPA) and granulated form (GRA) showed linear responses in the range of 0.288-0.672 and 0.4-1.2 µg/ml, respectively. The precision and accuracy were determined for the EPA solution at a concentration of 100 µg/ml. The spectrophotometric method performed well in quantifying the caffeine and total polyphenols.Instituto de Tecnologia do Paraná - Tecpar2008-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300002Brazilian Archives of Biology and Technology v.51 n.3 2008reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132008000300002info:eu-repo/semantics/openAccessPelozo,Maria Inez de GodoyCardoso,Mara Lane CarvalhoMello,João Carlos Palazzo deeng2008-07-16T00:00:00Zoai:scielo:S1516-89132008000300002Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2008-07-16T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Spectrophotometric determination of tannins and caffeine in preparations from Paullinia cupana var. sorbilis
title Spectrophotometric determination of tannins and caffeine in preparations from Paullinia cupana var. sorbilis
spellingShingle Spectrophotometric determination of tannins and caffeine in preparations from Paullinia cupana var. sorbilis
Pelozo,Maria Inez de Godoy
Caffeine
Paullinia cupana
Total polyphenols
Spectrophotometric quantification assay
title_short Spectrophotometric determination of tannins and caffeine in preparations from Paullinia cupana var. sorbilis
title_full Spectrophotometric determination of tannins and caffeine in preparations from Paullinia cupana var. sorbilis
title_fullStr Spectrophotometric determination of tannins and caffeine in preparations from Paullinia cupana var. sorbilis
title_full_unstemmed Spectrophotometric determination of tannins and caffeine in preparations from Paullinia cupana var. sorbilis
title_sort Spectrophotometric determination of tannins and caffeine in preparations from Paullinia cupana var. sorbilis
author Pelozo,Maria Inez de Godoy
author_facet Pelozo,Maria Inez de Godoy
Cardoso,Mara Lane Carvalho
Mello,João Carlos Palazzo de
author_role author
author2 Cardoso,Mara Lane Carvalho
Mello,João Carlos Palazzo de
author2_role author
author
dc.contributor.author.fl_str_mv Pelozo,Maria Inez de Godoy
Cardoso,Mara Lane Carvalho
Mello,João Carlos Palazzo de
dc.subject.por.fl_str_mv Caffeine
Paullinia cupana
Total polyphenols
Spectrophotometric quantification assay
topic Caffeine
Paullinia cupana
Total polyphenols
Spectrophotometric quantification assay
description The present work was designed to quantify the caffeine and total polyphenols in the extractive solution and the granulated form from the seeds of Paullinia cupana var. sorbilis, by the spectrophotometric method. The method showed linearity for the caffeine and polyphenols in the range of 5-25 µg/ml and 2.4-5.6 µg/ml respectively. The solutions of the semipurified fraction (EPA) and granulated form (GRA) showed linear responses in the range of 0.288-0.672 and 0.4-1.2 µg/ml, respectively. The precision and accuracy were determined for the EPA solution at a concentration of 100 µg/ml. The spectrophotometric method performed well in quantifying the caffeine and total polyphenols.
publishDate 2008
dc.date.none.fl_str_mv 2008-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300002
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300002
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132008000300002
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.51 n.3 2008
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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