Embryonic stem-like cells derived from in vitro produced bovine blastocysts
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300009 |
Resumo: | The aim of this work was to study the derivation of bovine embryonic stem-like (ES-like) cells from the inner cell mass (ICM) of in vitro produced blastocysts. The ICMs were mechanically isolated and six out of seventeen (35%) ICMs could attach to a monolayer of murine embryonic fibroblasts (MEF). Ten days after, primary outgrowths were mechanically dissected into several small clumps and transferred to a new MEF layer. Cells were further propagated and passaged by physical dissociation over a 60 days period. The pluripotency of the bovine ES-like cells was confirmed by RT-PCR of Oct-4 and STAT-3 gene markers. The colonies were weakly stained for alkaline phosphatase and the mesoderm and endoderm differentiation gene markers such as GATA-4 and Flk-1, respectively, were not expressed. Embryoid bodies were spontaneously formed at the seventh passage. Results showed that bovine ES-like cells could be obtained and passaged by mechanical procedures from the fresh in vitro produced blastocysts. |
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Brazilian Archives of Biology and Technology |
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Embryonic stem-like cells derived from in vitro produced bovine blastocystsBovine blastocystsICM isolationEmbryonic stem cellsGene markersThe aim of this work was to study the derivation of bovine embryonic stem-like (ES-like) cells from the inner cell mass (ICM) of in vitro produced blastocysts. The ICMs were mechanically isolated and six out of seventeen (35%) ICMs could attach to a monolayer of murine embryonic fibroblasts (MEF). Ten days after, primary outgrowths were mechanically dissected into several small clumps and transferred to a new MEF layer. Cells were further propagated and passaged by physical dissociation over a 60 days period. The pluripotency of the bovine ES-like cells was confirmed by RT-PCR of Oct-4 and STAT-3 gene markers. The colonies were weakly stained for alkaline phosphatase and the mesoderm and endoderm differentiation gene markers such as GATA-4 and Flk-1, respectively, were not expressed. Embryoid bodies were spontaneously formed at the seventh passage. Results showed that bovine ES-like cells could be obtained and passaged by mechanical procedures from the fresh in vitro produced blastocysts.Instituto de Tecnologia do Paraná - Tecpar2011-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300009Brazilian Archives of Biology and Technology v.54 n.3 2011reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132011000300009info:eu-repo/semantics/openAccessFreitas,Erika Regina Leal deSanches,Bruno ValenteGambarini,Maria LúciaOliveira Filho,Benedito DiasGuillo,Lidia Andreueng2011-06-13T00:00:00Zoai:scielo:S1516-89132011000300009Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2011-06-13T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Embryonic stem-like cells derived from in vitro produced bovine blastocysts |
title |
Embryonic stem-like cells derived from in vitro produced bovine blastocysts |
spellingShingle |
Embryonic stem-like cells derived from in vitro produced bovine blastocysts Freitas,Erika Regina Leal de Bovine blastocysts ICM isolation Embryonic stem cells Gene markers |
title_short |
Embryonic stem-like cells derived from in vitro produced bovine blastocysts |
title_full |
Embryonic stem-like cells derived from in vitro produced bovine blastocysts |
title_fullStr |
Embryonic stem-like cells derived from in vitro produced bovine blastocysts |
title_full_unstemmed |
Embryonic stem-like cells derived from in vitro produced bovine blastocysts |
title_sort |
Embryonic stem-like cells derived from in vitro produced bovine blastocysts |
author |
Freitas,Erika Regina Leal de |
author_facet |
Freitas,Erika Regina Leal de Sanches,Bruno Valente Gambarini,Maria Lúcia Oliveira Filho,Benedito Dias Guillo,Lidia Andreu |
author_role |
author |
author2 |
Sanches,Bruno Valente Gambarini,Maria Lúcia Oliveira Filho,Benedito Dias Guillo,Lidia Andreu |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Freitas,Erika Regina Leal de Sanches,Bruno Valente Gambarini,Maria Lúcia Oliveira Filho,Benedito Dias Guillo,Lidia Andreu |
dc.subject.por.fl_str_mv |
Bovine blastocysts ICM isolation Embryonic stem cells Gene markers |
topic |
Bovine blastocysts ICM isolation Embryonic stem cells Gene markers |
description |
The aim of this work was to study the derivation of bovine embryonic stem-like (ES-like) cells from the inner cell mass (ICM) of in vitro produced blastocysts. The ICMs were mechanically isolated and six out of seventeen (35%) ICMs could attach to a monolayer of murine embryonic fibroblasts (MEF). Ten days after, primary outgrowths were mechanically dissected into several small clumps and transferred to a new MEF layer. Cells were further propagated and passaged by physical dissociation over a 60 days period. The pluripotency of the bovine ES-like cells was confirmed by RT-PCR of Oct-4 and STAT-3 gene markers. The colonies were weakly stained for alkaline phosphatase and the mesoderm and endoderm differentiation gene markers such as GATA-4 and Flk-1, respectively, were not expressed. Embryoid bodies were spontaneously formed at the seventh passage. Results showed that bovine ES-like cells could be obtained and passaged by mechanical procedures from the fresh in vitro produced blastocysts. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300009 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300009 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-89132011000300009 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.54 n.3 2011 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318274494595072 |