Influence of culture medium and age of bovine blastocysts in established colonies of embryonic stem cells

• Autor(a) principal: Guastali, Midyan Daroz [UNESP]
• Data de Publicação: 2014
• Outros Autores: Monteiro, Bianca Andriolo [UNESP], Maziero, Rosiara Rosária Dias [UNESP], Paschoal, Daniela Martins [UNESP], Sudano, Mateus José [UNESP], Rascado, Tatiana da Silva [UNESP], Neto, João Ferreira Lima [UNESP], Vergara, Luis Eduardo [UNESP], Crocomo, Letícia Ferrrari [UNESP], Maserati, Marc, Landim-Alvarenga, Fernanda da Cruz [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/227988
• topic: BFGF; Blastocyst; Bovine; Characterization; Embryonic stem cells; Fetal calf serum; LIF

The objective of this study was to evaluate the potential of bovine IVF blastocysts at different stages of embryonic development in establishing ESC-like. Furthermore, blastocysts cultured in medium containing (10%) and (2.5%) fetal calf serum (FCS) were compared to determine if the serum concentration during in vitro culture alters the blastocyst’s potential to establish ESC-like culture. It was observed that only ICM’s from expanded blastocysts adhered to the monolayer (n=160) independent of the concentration of serum used during IVF culture. There were no observable differences in potential to establish ESC-like in embryos cultured with 2,5% or 10% FCS . The bFGF didn´t seems to be required for maintenance of bovine ESClike regardless of culture conditions. Blastocysts and colonies in primary culture and after the first passage were positive for Oct4, Nanog, SSEA-3 and TRA-1-81. Expanded blastocysts gave rise to ESC-like colonies, and the addition of LIF was sufficient to maintain cells undifferentiated in culture.