Investigation of toxic factors affecting cells of rat brains exposed to 3-methylcatechol

Detalhes bibliográficos
Autor(a) principal: Barreto,George Emílio Sampaio
Data de Publicação: 2007
Outros Autores: Oliveira,Elineusa Silva de, Villar,Flávio Augusto Guerreiro Aragão de, Santos,Gleide Souza dos, Lima,Rute Maria Ferreira, Costa,Silvia Lima, Costa,Maria de Fátima Dias, Egito,Eryvaldo Sócrates Tabosa, El-Bachá,Ramon Santos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000500012
Resumo: The aim of this work was to study the effects of 3MC on the peroxidation of biomolecules in nuclear fractions and nonsynaptic mitochondrial respiration in organelles obtained from rat brains. The cytotoxicity towards rat primary astrocytes in vitro was also tested. 3MC at 1mM oxidized consuming oxygen at a rate of 1.98 ± 0.19 µM.min-1 and formed reactive quinones. At the same concentration, 3MC induced peroxidation of biomolecules in nuclear fractions obtained from rat brain homogenates and inhibited state 2 FADH2-linked respiration in nonsynaptic mitochondria. Furthermore, 3MC oxidized in the culture medium, leading to the formation of quinones. This toluene metabolite was cytotoxic to rat primary astrocytes. The concentration that killed 50% of cells after 72 h was 107 mM. The results of the study indicated a direct relationship between cytotoxicity and 3MC oxidation.
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spelling Investigation of toxic factors affecting cells of rat brains exposed to 3-methylcatecholAstrocytesbraincytotoxicity3-methylcatecholperoxidationrespirationThe aim of this work was to study the effects of 3MC on the peroxidation of biomolecules in nuclear fractions and nonsynaptic mitochondrial respiration in organelles obtained from rat brains. The cytotoxicity towards rat primary astrocytes in vitro was also tested. 3MC at 1mM oxidized consuming oxygen at a rate of 1.98 ± 0.19 µM.min-1 and formed reactive quinones. At the same concentration, 3MC induced peroxidation of biomolecules in nuclear fractions obtained from rat brain homogenates and inhibited state 2 FADH2-linked respiration in nonsynaptic mitochondria. Furthermore, 3MC oxidized in the culture medium, leading to the formation of quinones. This toluene metabolite was cytotoxic to rat primary astrocytes. The concentration that killed 50% of cells after 72 h was 107 mM. The results of the study indicated a direct relationship between cytotoxicity and 3MC oxidation.Instituto de Tecnologia do Paraná - Tecpar2007-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000500012Brazilian Archives of Biology and Technology v.50 n.5 2007reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132007000500012info:eu-repo/semantics/openAccessBarreto,George Emílio SampaioOliveira,Elineusa Silva deVillar,Flávio Augusto Guerreiro Aragão deSantos,Gleide Souza dosLima,Rute Maria FerreiraCosta,Silvia LimaCosta,Maria de Fátima DiasEgito,Eryvaldo Sócrates TabosaEl-Bachá,Ramon Santoseng2007-11-23T00:00:00Zoai:scielo:S1516-89132007000500012Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2007-11-23T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Investigation of toxic factors affecting cells of rat brains exposed to 3-methylcatechol
title Investigation of toxic factors affecting cells of rat brains exposed to 3-methylcatechol
spellingShingle Investigation of toxic factors affecting cells of rat brains exposed to 3-methylcatechol
Barreto,George Emílio Sampaio
Astrocytes
brain
cytotoxicity
3-methylcatechol
peroxidation
respiration
title_short Investigation of toxic factors affecting cells of rat brains exposed to 3-methylcatechol
title_full Investigation of toxic factors affecting cells of rat brains exposed to 3-methylcatechol
title_fullStr Investigation of toxic factors affecting cells of rat brains exposed to 3-methylcatechol
title_full_unstemmed Investigation of toxic factors affecting cells of rat brains exposed to 3-methylcatechol
title_sort Investigation of toxic factors affecting cells of rat brains exposed to 3-methylcatechol
author Barreto,George Emílio Sampaio
author_facet Barreto,George Emílio Sampaio
Oliveira,Elineusa Silva de
Villar,Flávio Augusto Guerreiro Aragão de
Santos,Gleide Souza dos
Lima,Rute Maria Ferreira
Costa,Silvia Lima
Costa,Maria de Fátima Dias
Egito,Eryvaldo Sócrates Tabosa
El-Bachá,Ramon Santos
author_role author
author2 Oliveira,Elineusa Silva de
Villar,Flávio Augusto Guerreiro Aragão de
Santos,Gleide Souza dos
Lima,Rute Maria Ferreira
Costa,Silvia Lima
Costa,Maria de Fátima Dias
Egito,Eryvaldo Sócrates Tabosa
El-Bachá,Ramon Santos
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Barreto,George Emílio Sampaio
Oliveira,Elineusa Silva de
Villar,Flávio Augusto Guerreiro Aragão de
Santos,Gleide Souza dos
Lima,Rute Maria Ferreira
Costa,Silvia Lima
Costa,Maria de Fátima Dias
Egito,Eryvaldo Sócrates Tabosa
El-Bachá,Ramon Santos
dc.subject.por.fl_str_mv Astrocytes
brain
cytotoxicity
3-methylcatechol
peroxidation
respiration
topic Astrocytes
brain
cytotoxicity
3-methylcatechol
peroxidation
respiration
description The aim of this work was to study the effects of 3MC on the peroxidation of biomolecules in nuclear fractions and nonsynaptic mitochondrial respiration in organelles obtained from rat brains. The cytotoxicity towards rat primary astrocytes in vitro was also tested. 3MC at 1mM oxidized consuming oxygen at a rate of 1.98 ± 0.19 µM.min-1 and formed reactive quinones. At the same concentration, 3MC induced peroxidation of biomolecules in nuclear fractions obtained from rat brain homogenates and inhibited state 2 FADH2-linked respiration in nonsynaptic mitochondria. Furthermore, 3MC oxidized in the culture medium, leading to the formation of quinones. This toluene metabolite was cytotoxic to rat primary astrocytes. The concentration that killed 50% of cells after 72 h was 107 mM. The results of the study indicated a direct relationship between cytotoxicity and 3MC oxidation.
publishDate 2007
dc.date.none.fl_str_mv 2007-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000500012
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000500012
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132007000500012
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.50 n.5 2007
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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