Hydrolysis of whey lactose by immobilized β-Galactosidase

Detalhes bibliográficos
Autor(a) principal: Mariotti,Marcela Panaro
Data de Publicação: 2008
Outros Autores: Yamanaka,Hideko, Araujo,Angela Regina, Trevisan,Henrique Celso
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600019
Resumo: Hydrolysis of whey lactose to glucose and galactose by immobilized galactosidase comes as an alternative to enlarge the possibilities of commercial use of this feedstock. To be applied at industrial scale, the process should be performed continuously .This work aimed to study the hydrolysis of whey lactose by an immobilized enzyme reactor. b-Galactosidase from Aspergillus oryzae was immobilized on silica and activity and stability were evaluated. The best immobilization results were attained by using glutaraldehyde as support's activator and enzyme stabilizer. The optimized enzyme proportion for immobilization was 15-20 mg g-1 of support. Treatments of whey were performed (microfiltration, thermal treatment and ultrafiltration), seeking the elimination of sludge, and the effects on operating the fixed bed reactor were evaluated. Ultrafiltration was the best treatment towards a proper substrate solution for feeding the reactor.
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spelling Hydrolysis of whey lactose by immobilized β-Galactosidaseβ-galactosidaseimmobilizationlactasewheylactosehydrolysisHydrolysis of whey lactose to glucose and galactose by immobilized galactosidase comes as an alternative to enlarge the possibilities of commercial use of this feedstock. To be applied at industrial scale, the process should be performed continuously .This work aimed to study the hydrolysis of whey lactose by an immobilized enzyme reactor. b-Galactosidase from Aspergillus oryzae was immobilized on silica and activity and stability were evaluated. The best immobilization results were attained by using glutaraldehyde as support's activator and enzyme stabilizer. The optimized enzyme proportion for immobilization was 15-20 mg g-1 of support. Treatments of whey were performed (microfiltration, thermal treatment and ultrafiltration), seeking the elimination of sludge, and the effects on operating the fixed bed reactor were evaluated. Ultrafiltration was the best treatment towards a proper substrate solution for feeding the reactor.Instituto de Tecnologia do Paraná - Tecpar2008-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600019Brazilian Archives of Biology and Technology v.51 n.6 2008reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132008000600019info:eu-repo/semantics/openAccessMariotti,Marcela PanaroYamanaka,HidekoAraujo,Angela ReginaTrevisan,Henrique Celsoeng2009-01-27T00:00:00Zoai:scielo:S1516-89132008000600019Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2009-01-27T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Hydrolysis of whey lactose by immobilized β-Galactosidase
title Hydrolysis of whey lactose by immobilized β-Galactosidase
spellingShingle Hydrolysis of whey lactose by immobilized β-Galactosidase
Mariotti,Marcela Panaro
β-galactosidase
immobilization
lactase
whey
lactose
hydrolysis
title_short Hydrolysis of whey lactose by immobilized β-Galactosidase
title_full Hydrolysis of whey lactose by immobilized β-Galactosidase
title_fullStr Hydrolysis of whey lactose by immobilized β-Galactosidase
title_full_unstemmed Hydrolysis of whey lactose by immobilized β-Galactosidase
title_sort Hydrolysis of whey lactose by immobilized β-Galactosidase
author Mariotti,Marcela Panaro
author_facet Mariotti,Marcela Panaro
Yamanaka,Hideko
Araujo,Angela Regina
Trevisan,Henrique Celso
author_role author
author2 Yamanaka,Hideko
Araujo,Angela Regina
Trevisan,Henrique Celso
author2_role author
author
author
dc.contributor.author.fl_str_mv Mariotti,Marcela Panaro
Yamanaka,Hideko
Araujo,Angela Regina
Trevisan,Henrique Celso
dc.subject.por.fl_str_mv β-galactosidase
immobilization
lactase
whey
lactose
hydrolysis
topic β-galactosidase
immobilization
lactase
whey
lactose
hydrolysis
description Hydrolysis of whey lactose to glucose and galactose by immobilized galactosidase comes as an alternative to enlarge the possibilities of commercial use of this feedstock. To be applied at industrial scale, the process should be performed continuously .This work aimed to study the hydrolysis of whey lactose by an immobilized enzyme reactor. b-Galactosidase from Aspergillus oryzae was immobilized on silica and activity and stability were evaluated. The best immobilization results were attained by using glutaraldehyde as support's activator and enzyme stabilizer. The optimized enzyme proportion for immobilization was 15-20 mg g-1 of support. Treatments of whey were performed (microfiltration, thermal treatment and ultrafiltration), seeking the elimination of sludge, and the effects on operating the fixed bed reactor were evaluated. Ultrafiltration was the best treatment towards a proper substrate solution for feeding the reactor.
publishDate 2008
dc.date.none.fl_str_mv 2008-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600019
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600019
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132008000600019
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.51 n.6 2008
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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