Application of Next-generation Sequencing in Clinical Molecular Diagnostics

Detalhes bibliográficos
Autor(a) principal: Seifi,Morteza
Data de Publicação: 2017
Outros Autores: Ghasemi,Asghar, Raeisi,Sina, Heidarzadeh,Siamak
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100303
Resumo: ABSTRACT Next-generation sequencing (NGS) is the catch all terms that used to explain several different modern sequencing technologies which let us to sequence nucleic acids much more rapidly and cheaply than the formerly used Sanger sequencing, and as such have revolutionized the study of molecular biology and genomics with excellent resolution and accuracy. Over the past years, many academic companies and institutions have continued technological advances to expand NGS applications from research to the clinic. In this review, the performance and technical features of current NGS platforms were described. Furthermore, advances in the applying of NGS technologies towards the progress of clinical molecular diagnostics were emphasized. General advantages and disadvantages of each sequencing system are summarized and compared to guide the selection of NGS platforms for specific research aims.
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spelling Application of Next-generation Sequencing in Clinical Molecular DiagnosticsBioinformaticsclinical molecular applicationsethical aspectsnext generation sequencingABSTRACT Next-generation sequencing (NGS) is the catch all terms that used to explain several different modern sequencing technologies which let us to sequence nucleic acids much more rapidly and cheaply than the formerly used Sanger sequencing, and as such have revolutionized the study of molecular biology and genomics with excellent resolution and accuracy. Over the past years, many academic companies and institutions have continued technological advances to expand NGS applications from research to the clinic. In this review, the performance and technical features of current NGS platforms were described. Furthermore, advances in the applying of NGS technologies towards the progress of clinical molecular diagnostics were emphasized. General advantages and disadvantages of each sequencing system are summarized and compared to guide the selection of NGS platforms for specific research aims.Instituto de Tecnologia do Paraná - Tecpar2017-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100303Brazilian Archives of Biology and Technology v.60 2017reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/1678-4324-2017160414info:eu-repo/semantics/openAccessSeifi,MortezaGhasemi,AsgharRaeisi,SinaHeidarzadeh,Siamakeng2017-05-15T00:00:00Zoai:scielo:S1516-89132017000100303Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2017-05-15T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Application of Next-generation Sequencing in Clinical Molecular Diagnostics
title Application of Next-generation Sequencing in Clinical Molecular Diagnostics
spellingShingle Application of Next-generation Sequencing in Clinical Molecular Diagnostics
Seifi,Morteza
Bioinformatics
clinical molecular applications
ethical aspects
next generation sequencing
title_short Application of Next-generation Sequencing in Clinical Molecular Diagnostics
title_full Application of Next-generation Sequencing in Clinical Molecular Diagnostics
title_fullStr Application of Next-generation Sequencing in Clinical Molecular Diagnostics
title_full_unstemmed Application of Next-generation Sequencing in Clinical Molecular Diagnostics
title_sort Application of Next-generation Sequencing in Clinical Molecular Diagnostics
author Seifi,Morteza
author_facet Seifi,Morteza
Ghasemi,Asghar
Raeisi,Sina
Heidarzadeh,Siamak
author_role author
author2 Ghasemi,Asghar
Raeisi,Sina
Heidarzadeh,Siamak
author2_role author
author
author
dc.contributor.author.fl_str_mv Seifi,Morteza
Ghasemi,Asghar
Raeisi,Sina
Heidarzadeh,Siamak
dc.subject.por.fl_str_mv Bioinformatics
clinical molecular applications
ethical aspects
next generation sequencing
topic Bioinformatics
clinical molecular applications
ethical aspects
next generation sequencing
description ABSTRACT Next-generation sequencing (NGS) is the catch all terms that used to explain several different modern sequencing technologies which let us to sequence nucleic acids much more rapidly and cheaply than the formerly used Sanger sequencing, and as such have revolutionized the study of molecular biology and genomics with excellent resolution and accuracy. Over the past years, many academic companies and institutions have continued technological advances to expand NGS applications from research to the clinic. In this review, the performance and technical features of current NGS platforms were described. Furthermore, advances in the applying of NGS technologies towards the progress of clinical molecular diagnostics were emphasized. General advantages and disadvantages of each sequencing system are summarized and compared to guide the selection of NGS platforms for specific research aims.
publishDate 2017
dc.date.none.fl_str_mv 2017-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100303
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100303
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1678-4324-2017160414
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.60 2017
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
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reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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