Optimization of the production of extracellular α-amylase by Kluyveromyces marxianus IF0 0288 by response surface methodology
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300016 |
Resumo: | The aim of this work was to study the production of extracellular α-amylase by Kluyveromyces marxianus IF0 0288 using optimized nutritional and cultural conditions in a complex yeast medium under aerobic batch fermentation. By applying the conventional "one-variable-at-a-time" approach and the response surface methodology, the effect of four fermentation parameters (type of carbon source, initial culture pH, temperature, and incubation time) on the growth and α-amylase production was evaluated. The production of α-amylase during 60 h of fermentation increased 13-fold under optimized conditions (1% starch, pH 6.0, 30ºC) in comparison to the conventional optimization method. The initial pH value of 6.13 and temperature of 30.3ºC were optimal conditions by the response surface methodology, leading to further improvement (up to 13-fold) in the production of extracellular α-amylase. These results constituted first evidence that K. marxianus could be potentially used as an effective source of extracellular α-amylase. |
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Brazilian Archives of Biology and Technology |
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Optimization of the production of extracellular α-amylase by Kluyveromyces marxianus IF0 0288 by response surface methodologyExtracellular enzymesα-amylaseKluyveromyces marxianusfermentationRSMThe aim of this work was to study the production of extracellular α-amylase by Kluyveromyces marxianus IF0 0288 using optimized nutritional and cultural conditions in a complex yeast medium under aerobic batch fermentation. By applying the conventional "one-variable-at-a-time" approach and the response surface methodology, the effect of four fermentation parameters (type of carbon source, initial culture pH, temperature, and incubation time) on the growth and α-amylase production was evaluated. The production of α-amylase during 60 h of fermentation increased 13-fold under optimized conditions (1% starch, pH 6.0, 30ºC) in comparison to the conventional optimization method. The initial pH value of 6.13 and temperature of 30.3ºC were optimal conditions by the response surface methodology, leading to further improvement (up to 13-fold) in the production of extracellular α-amylase. These results constituted first evidence that K. marxianus could be potentially used as an effective source of extracellular α-amylase.Instituto de Tecnologia do Paraná - Tecpar2014-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300016Brazilian Archives of Biology and Technology v.57 n.3 2014reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-8913201401485info:eu-repo/semantics/openAccessStergiou,Panagiota-YiolandaFoukis,AthanasiosTheodorou,LeonidasPapagianni,MariaPapamichael,Emmanueleng2014-05-30T00:00:00Zoai:scielo:S1516-89132014000300016Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2014-05-30T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Optimization of the production of extracellular α-amylase by Kluyveromyces marxianus IF0 0288 by response surface methodology |
title |
Optimization of the production of extracellular α-amylase by Kluyveromyces marxianus IF0 0288 by response surface methodology |
spellingShingle |
Optimization of the production of extracellular α-amylase by Kluyveromyces marxianus IF0 0288 by response surface methodology Stergiou,Panagiota-Yiolanda Extracellular enzymes α-amylase Kluyveromyces marxianus fermentation RSM |
title_short |
Optimization of the production of extracellular α-amylase by Kluyveromyces marxianus IF0 0288 by response surface methodology |
title_full |
Optimization of the production of extracellular α-amylase by Kluyveromyces marxianus IF0 0288 by response surface methodology |
title_fullStr |
Optimization of the production of extracellular α-amylase by Kluyveromyces marxianus IF0 0288 by response surface methodology |
title_full_unstemmed |
Optimization of the production of extracellular α-amylase by Kluyveromyces marxianus IF0 0288 by response surface methodology |
title_sort |
Optimization of the production of extracellular α-amylase by Kluyveromyces marxianus IF0 0288 by response surface methodology |
author |
Stergiou,Panagiota-Yiolanda |
author_facet |
Stergiou,Panagiota-Yiolanda Foukis,Athanasios Theodorou,Leonidas Papagianni,Maria Papamichael,Emmanuel |
author_role |
author |
author2 |
Foukis,Athanasios Theodorou,Leonidas Papagianni,Maria Papamichael,Emmanuel |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Stergiou,Panagiota-Yiolanda Foukis,Athanasios Theodorou,Leonidas Papagianni,Maria Papamichael,Emmanuel |
dc.subject.por.fl_str_mv |
Extracellular enzymes α-amylase Kluyveromyces marxianus fermentation RSM |
topic |
Extracellular enzymes α-amylase Kluyveromyces marxianus fermentation RSM |
description |
The aim of this work was to study the production of extracellular α-amylase by Kluyveromyces marxianus IF0 0288 using optimized nutritional and cultural conditions in a complex yeast medium under aerobic batch fermentation. By applying the conventional "one-variable-at-a-time" approach and the response surface methodology, the effect of four fermentation parameters (type of carbon source, initial culture pH, temperature, and incubation time) on the growth and α-amylase production was evaluated. The production of α-amylase during 60 h of fermentation increased 13-fold under optimized conditions (1% starch, pH 6.0, 30ºC) in comparison to the conventional optimization method. The initial pH value of 6.13 and temperature of 30.3ºC were optimal conditions by the response surface methodology, leading to further improvement (up to 13-fold) in the production of extracellular α-amylase. These results constituted first evidence that K. marxianus could be potentially used as an effective source of extracellular α-amylase. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300016 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300016 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-8913201401485 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.57 n.3 2014 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318276133519360 |