Optimization of the production of extracellular α-amylase by Kluyveromyces marxianus IF0 0288 by response surface methodology

Detalhes bibliográficos
Autor(a) principal: Stergiou,Panagiota-Yiolanda
Data de Publicação: 2014
Outros Autores: Foukis,Athanasios, Theodorou,Leonidas, Papagianni,Maria, Papamichael,Emmanuel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300016
Resumo: The aim of this work was to study the production of extracellular α-amylase by Kluyveromyces marxianus IF0 0288 using optimized nutritional and cultural conditions in a complex yeast medium under aerobic batch fermentation. By applying the conventional "one-variable-at-a-time" approach and the response surface methodology, the effect of four fermentation parameters (type of carbon source, initial culture pH, temperature, and incubation time) on the growth and α-amylase production was evaluated. The production of α-amylase during 60 h of fermentation increased 13-fold under optimized conditions (1% starch, pH 6.0, 30ºC) in comparison to the conventional optimization method. The initial pH value of 6.13 and temperature of 30.3ºC were optimal conditions by the response surface methodology, leading to further improvement (up to 13-fold) in the production of extracellular α-amylase. These results constituted first evidence that K. marxianus could be potentially used as an effective source of extracellular α-amylase.
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spelling Optimization of the production of extracellular α-amylase by Kluyveromyces marxianus IF0 0288 by response surface methodologyExtracellular enzymesα-amylaseKluyveromyces marxianusfermentationRSMThe aim of this work was to study the production of extracellular α-amylase by Kluyveromyces marxianus IF0 0288 using optimized nutritional and cultural conditions in a complex yeast medium under aerobic batch fermentation. By applying the conventional "one-variable-at-a-time" approach and the response surface methodology, the effect of four fermentation parameters (type of carbon source, initial culture pH, temperature, and incubation time) on the growth and α-amylase production was evaluated. The production of α-amylase during 60 h of fermentation increased 13-fold under optimized conditions (1% starch, pH 6.0, 30ºC) in comparison to the conventional optimization method. The initial pH value of 6.13 and temperature of 30.3ºC were optimal conditions by the response surface methodology, leading to further improvement (up to 13-fold) in the production of extracellular α-amylase. These results constituted first evidence that K. marxianus could be potentially used as an effective source of extracellular α-amylase.Instituto de Tecnologia do Paraná - Tecpar2014-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300016Brazilian Archives of Biology and Technology v.57 n.3 2014reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-8913201401485info:eu-repo/semantics/openAccessStergiou,Panagiota-YiolandaFoukis,AthanasiosTheodorou,LeonidasPapagianni,MariaPapamichael,Emmanueleng2014-05-30T00:00:00Zoai:scielo:S1516-89132014000300016Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2014-05-30T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Optimization of the production of extracellular α-amylase by Kluyveromyces marxianus IF0 0288 by response surface methodology
title Optimization of the production of extracellular α-amylase by Kluyveromyces marxianus IF0 0288 by response surface methodology
spellingShingle Optimization of the production of extracellular α-amylase by Kluyveromyces marxianus IF0 0288 by response surface methodology
Stergiou,Panagiota-Yiolanda
Extracellular enzymes
α-amylase
Kluyveromyces marxianus
fermentation
RSM
title_short Optimization of the production of extracellular α-amylase by Kluyveromyces marxianus IF0 0288 by response surface methodology
title_full Optimization of the production of extracellular α-amylase by Kluyveromyces marxianus IF0 0288 by response surface methodology
title_fullStr Optimization of the production of extracellular α-amylase by Kluyveromyces marxianus IF0 0288 by response surface methodology
title_full_unstemmed Optimization of the production of extracellular α-amylase by Kluyveromyces marxianus IF0 0288 by response surface methodology
title_sort Optimization of the production of extracellular α-amylase by Kluyveromyces marxianus IF0 0288 by response surface methodology
author Stergiou,Panagiota-Yiolanda
author_facet Stergiou,Panagiota-Yiolanda
Foukis,Athanasios
Theodorou,Leonidas
Papagianni,Maria
Papamichael,Emmanuel
author_role author
author2 Foukis,Athanasios
Theodorou,Leonidas
Papagianni,Maria
Papamichael,Emmanuel
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Stergiou,Panagiota-Yiolanda
Foukis,Athanasios
Theodorou,Leonidas
Papagianni,Maria
Papamichael,Emmanuel
dc.subject.por.fl_str_mv Extracellular enzymes
α-amylase
Kluyveromyces marxianus
fermentation
RSM
topic Extracellular enzymes
α-amylase
Kluyveromyces marxianus
fermentation
RSM
description The aim of this work was to study the production of extracellular α-amylase by Kluyveromyces marxianus IF0 0288 using optimized nutritional and cultural conditions in a complex yeast medium under aerobic batch fermentation. By applying the conventional "one-variable-at-a-time" approach and the response surface methodology, the effect of four fermentation parameters (type of carbon source, initial culture pH, temperature, and incubation time) on the growth and α-amylase production was evaluated. The production of α-amylase during 60 h of fermentation increased 13-fold under optimized conditions (1% starch, pH 6.0, 30ºC) in comparison to the conventional optimization method. The initial pH value of 6.13 and temperature of 30.3ºC were optimal conditions by the response surface methodology, leading to further improvement (up to 13-fold) in the production of extracellular α-amylase. These results constituted first evidence that K. marxianus could be potentially used as an effective source of extracellular α-amylase.
publishDate 2014
dc.date.none.fl_str_mv 2014-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300016
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300016
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-8913201401485
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.57 n.3 2014
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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