Xylanase production by Streptomyces viridosporus T7A in submerged and solid-state fermentation using agro-industrial residues

• Autor(a) principal: Alberton,Luiz Romulo
• Data de Publicação: 2009
• Outros Autores: Vandenberghe,Luciana Porto de Souza, Assmann,Ricardo, Fendrich,Ricardo Cancio, Rodriguéz-León,José, Soccol,Carlos Ricardo
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Brazilian Archives of Biology and Technology
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700022
• topic: Streptomyces; xylanase; submerged fermentation; solid-state agro-industrial fermentation; residues

The study of xylanase production was conducted by Streptomyces viridosporus T7A in submerged (SmF) and solid-state fermentation (SSF), using agro-industrial residues and sub-products. Napier grass, sugarcane bagasse and soybean bran were used as carbon source, substrate/support, and nitrogen source, respectively. In SmF, Napier grass (1% v/w) supplemented with soybean bran, hydroxyethylcellulose and B complex vitamins were used. Soybean bran (1.5 % w/v), B complex vitamins (0.1%), and hydroxyethilcellulose (0.15%) led to an increase in xylanase production (23.41 U/mL). In SSF, the effects of the following parameters were studied: substrate composition (sugarcane bagasse, Napier grass and soybean bran), initial moisture, and inoculum rate. In SSF, the highest xylanase activity (423.9 U/g) was reached with: 70 % sugarcane bagasse, 20% Napier grass and 10% soybean meal, 90% of moisture, and 10(7)/g substrate.