Evaluation of gelling agents on anther culture: response of two soybean cultivars
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000700004 |
Resumo: | Anthers of two soybean cultivars were cultured in B5 long basal culture media gelled with agarose or PhytagelTM. Cytological examinations of the anthers were carried out during the first 45 days of culture to assay the viability and developmental stage of microspores. Frequency of callus formation was recorded at 45 days of culture. The analysis of variance of the microspore viability assay showed significant Cultivar X Gelling Agent X Day of Culture interactions. The frequencies of viable microspores decreased significantly with time of culture, within each cultivar and gelling agent tested. The interaction Day X Cultivar was significant for the frequencies of binucleate symmetrical grains and multinucleate/multicellular structures. The effect of gelling agents on the frequency of binucleate symmetrical pollens grains and multinucleate/multicellular structures was not significant. About the frequencies of calli and embryogenic calli formed, a significant difference was detected between the cultivars (IAS5= 14.8% and BRS 133=6.6%). Gelling agents showed no effect over these frequencies. |
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Evaluation of gelling agents on anther culture: response of two soybean cultivarsAnther cultureGlycine maxculture mediabinucleate pollen grainsmultinucleate/multicellular structurescallus formationAnthers of two soybean cultivars were cultured in B5 long basal culture media gelled with agarose or PhytagelTM. Cytological examinations of the anthers were carried out during the first 45 days of culture to assay the viability and developmental stage of microspores. Frequency of callus formation was recorded at 45 days of culture. The analysis of variance of the microspore viability assay showed significant Cultivar X Gelling Agent X Day of Culture interactions. The frequencies of viable microspores decreased significantly with time of culture, within each cultivar and gelling agent tested. The interaction Day X Cultivar was significant for the frequencies of binucleate symmetrical grains and multinucleate/multicellular structures. The effect of gelling agents on the frequency of binucleate symmetrical pollens grains and multinucleate/multicellular structures was not significant. About the frequencies of calli and embryogenic calli formed, a significant difference was detected between the cultivars (IAS5= 14.8% and BRS 133=6.6%). Gelling agents showed no effect over these frequencies.Instituto de Tecnologia do Paraná - Tecpar2007-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000700004Brazilian Archives of Biology and Technology v.50 n.6 2007reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132007000700004info:eu-repo/semantics/openAccessCardoso,Milena BarcelosBodanese-Zanettini,Maria HelenaMundstock,Elsa Cristina deKaltchuk-Santos,Elianeeng2008-03-03T00:00:00Zoai:scielo:S1516-89132007000700004Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2008-03-03T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Evaluation of gelling agents on anther culture: response of two soybean cultivars |
title |
Evaluation of gelling agents on anther culture: response of two soybean cultivars |
spellingShingle |
Evaluation of gelling agents on anther culture: response of two soybean cultivars Cardoso,Milena Barcelos Anther culture Glycine max culture media binucleate pollen grains multinucleate/multicellular structures callus formation |
title_short |
Evaluation of gelling agents on anther culture: response of two soybean cultivars |
title_full |
Evaluation of gelling agents on anther culture: response of two soybean cultivars |
title_fullStr |
Evaluation of gelling agents on anther culture: response of two soybean cultivars |
title_full_unstemmed |
Evaluation of gelling agents on anther culture: response of two soybean cultivars |
title_sort |
Evaluation of gelling agents on anther culture: response of two soybean cultivars |
author |
Cardoso,Milena Barcelos |
author_facet |
Cardoso,Milena Barcelos Bodanese-Zanettini,Maria Helena Mundstock,Elsa Cristina de Kaltchuk-Santos,Eliane |
author_role |
author |
author2 |
Bodanese-Zanettini,Maria Helena Mundstock,Elsa Cristina de Kaltchuk-Santos,Eliane |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Cardoso,Milena Barcelos Bodanese-Zanettini,Maria Helena Mundstock,Elsa Cristina de Kaltchuk-Santos,Eliane |
dc.subject.por.fl_str_mv |
Anther culture Glycine max culture media binucleate pollen grains multinucleate/multicellular structures callus formation |
topic |
Anther culture Glycine max culture media binucleate pollen grains multinucleate/multicellular structures callus formation |
description |
Anthers of two soybean cultivars were cultured in B5 long basal culture media gelled with agarose or PhytagelTM. Cytological examinations of the anthers were carried out during the first 45 days of culture to assay the viability and developmental stage of microspores. Frequency of callus formation was recorded at 45 days of culture. The analysis of variance of the microspore viability assay showed significant Cultivar X Gelling Agent X Day of Culture interactions. The frequencies of viable microspores decreased significantly with time of culture, within each cultivar and gelling agent tested. The interaction Day X Cultivar was significant for the frequencies of binucleate symmetrical grains and multinucleate/multicellular structures. The effect of gelling agents on the frequency of binucleate symmetrical pollens grains and multinucleate/multicellular structures was not significant. About the frequencies of calli and embryogenic calli formed, a significant difference was detected between the cultivars (IAS5= 14.8% and BRS 133=6.6%). Gelling agents showed no effect over these frequencies. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-11-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000700004 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000700004 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-89132007000700004 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.50 n.6 2007 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318271879446528 |