Initial segmentation patterns of microspores and pollen viability in soybean cultured anthers: indication of chromosome doubling

• Autor(a) principal: Cardoso,Milena Barcelos
• Data de Publicação: 2004
• Outros Autores: Kaltchuk-Santos,Eliane, Mundstock,Elsa Cristina de, Bodanese-Zanettini,Maria Helena
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Brazilian Archives of Biology and Technology
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500005
• topic: Glycine max; FDA; binucleate pollen grains; multinucleate pollen grains; spontaneous diploidization

Anthers obtained from flowers buds of soybean cultivar IAS-5 were cultured in two basal culture media (B5 and B5 long). Cytological examinations of the in vitro anthers were performed during the first 20 days of culture to assay the viability (by propionic-carmine and fluorescein diacetate tests) and the stage of development of pollen grains. The frequencies of viable pollen grains varied significantly between bud sizes on the propionic-carmine analysis. The basal culture media and bud size had no clear effect on the frequencies of binucleate symmetrical and multinucleate pollen grains. Chromosome counts of metaphasic microspores throughout the culture period showed microspores with higher ploidy level in addition to normal chromosome number (n=20).