Uncaria tomentosa extract: evaluation of effects on the in vitro and in vivo labeling of blood constituents with technetium-99m
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700024 |
Resumo: | The influence (in vivo and in vitro) of an Uncaria tomentosa extract (Cats claw) on the labeling of red blood cells (RBCs) and plasma and cellular proteins with technetium-99m (Tc-99m) was evaluated. For the in vivo treatment, animals were treated with Cats claw. For the in vitro treatment, heparinized blood was incubated with Cats claw before the addition of stannous chloride (SnCl2) and Tc-99m. Samples of plasma (P) and RBCs were separated and also precipitated with trichloroacetic acid. The soluble and insoluble fractions of P and RBCs were isolated. The analysis of the results of the in vivo study, indicates that there is no significant alteration on the uptake of Tc-99m by the blood constituents, but it significantly decrease (p<0.05) the labeling of blood constituents by in vitro methods. These effects could be due to chelation of stannous and /or pertechnetate ions and blockage of the Tc-99m bindings sites. |
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Uncaria tomentosa extract: evaluation of effects on the in vitro and in vivo labeling of blood constituents with technetium-99mUncaria tomentosatechnetium-99mradiolabelingblood constituentsThe influence (in vivo and in vitro) of an Uncaria tomentosa extract (Cats claw) on the labeling of red blood cells (RBCs) and plasma and cellular proteins with technetium-99m (Tc-99m) was evaluated. For the in vivo treatment, animals were treated with Cats claw. For the in vitro treatment, heparinized blood was incubated with Cats claw before the addition of stannous chloride (SnCl2) and Tc-99m. Samples of plasma (P) and RBCs were separated and also precipitated with trichloroacetic acid. The soluble and insoluble fractions of P and RBCs were isolated. The analysis of the results of the in vivo study, indicates that there is no significant alteration on the uptake of Tc-99m by the blood constituents, but it significantly decrease (p<0.05) the labeling of blood constituents by in vitro methods. These effects could be due to chelation of stannous and /or pertechnetate ions and blockage of the Tc-99m bindings sites.Instituto de Tecnologia do Paraná - Tecpar2008-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700024Brazilian Archives of Biology and Technology v.51 n.spe 2008reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132008000700024info:eu-repo/semantics/openAccessMoreno,Silvana Ramos FariasCarvalho,Jorge José deNascimento,Ana LúciaOlej,BeniRocha,Emely KazanArnobio,AdrianoBernardo-Filho,MárioCaldas,Luiz Querino de AraújoHoneycut,Haydeneng2009-02-20T00:00:00Zoai:scielo:S1516-89132008000700024Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2009-02-20T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Uncaria tomentosa extract: evaluation of effects on the in vitro and in vivo labeling of blood constituents with technetium-99m |
title |
Uncaria tomentosa extract: evaluation of effects on the in vitro and in vivo labeling of blood constituents with technetium-99m |
spellingShingle |
Uncaria tomentosa extract: evaluation of effects on the in vitro and in vivo labeling of blood constituents with technetium-99m Moreno,Silvana Ramos Farias Uncaria tomentosa technetium-99m radiolabeling blood constituents |
title_short |
Uncaria tomentosa extract: evaluation of effects on the in vitro and in vivo labeling of blood constituents with technetium-99m |
title_full |
Uncaria tomentosa extract: evaluation of effects on the in vitro and in vivo labeling of blood constituents with technetium-99m |
title_fullStr |
Uncaria tomentosa extract: evaluation of effects on the in vitro and in vivo labeling of blood constituents with technetium-99m |
title_full_unstemmed |
Uncaria tomentosa extract: evaluation of effects on the in vitro and in vivo labeling of blood constituents with technetium-99m |
title_sort |
Uncaria tomentosa extract: evaluation of effects on the in vitro and in vivo labeling of blood constituents with technetium-99m |
author |
Moreno,Silvana Ramos Farias |
author_facet |
Moreno,Silvana Ramos Farias Carvalho,Jorge José de Nascimento,Ana Lúcia Olej,Beni Rocha,Emely Kazan Arnobio,Adriano Bernardo-Filho,Mário Caldas,Luiz Querino de Araújo Honeycut,Hayden |
author_role |
author |
author2 |
Carvalho,Jorge José de Nascimento,Ana Lúcia Olej,Beni Rocha,Emely Kazan Arnobio,Adriano Bernardo-Filho,Mário Caldas,Luiz Querino de Araújo Honeycut,Hayden |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Moreno,Silvana Ramos Farias Carvalho,Jorge José de Nascimento,Ana Lúcia Olej,Beni Rocha,Emely Kazan Arnobio,Adriano Bernardo-Filho,Mário Caldas,Luiz Querino de Araújo Honeycut,Hayden |
dc.subject.por.fl_str_mv |
Uncaria tomentosa technetium-99m radiolabeling blood constituents |
topic |
Uncaria tomentosa technetium-99m radiolabeling blood constituents |
description |
The influence (in vivo and in vitro) of an Uncaria tomentosa extract (Cats claw) on the labeling of red blood cells (RBCs) and plasma and cellular proteins with technetium-99m (Tc-99m) was evaluated. For the in vivo treatment, animals were treated with Cats claw. For the in vitro treatment, heparinized blood was incubated with Cats claw before the addition of stannous chloride (SnCl2) and Tc-99m. Samples of plasma (P) and RBCs were separated and also precipitated with trichloroacetic acid. The soluble and insoluble fractions of P and RBCs were isolated. The analysis of the results of the in vivo study, indicates that there is no significant alteration on the uptake of Tc-99m by the blood constituents, but it significantly decrease (p<0.05) the labeling of blood constituents by in vitro methods. These effects could be due to chelation of stannous and /or pertechnetate ions and blockage of the Tc-99m bindings sites. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700024 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700024 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-89132008000700024 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.51 n.spe 2008 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318272688947200 |