Light microscope observation of circulating human lymphocytes cultured in vitro

Detalhes bibliográficos
Autor(a) principal: Oliveira,Naila Francis Paulo de
Data de Publicação: 2010
Outros Autores: Dolder,Mary Anne Heidi, Genari,Selma Candelária
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500013
Resumo: The purpose of this work was to study the isolation and a light microscopy technique for cultured lymphocytes. Blood samples were obtained by venipuncture with an anticoagulant added and centrifuged in a Percoll density gradient to separate the leukocytes. Lymphocytes were placed in 25 cm ³ tissue culture flasks at 37ºC. After culturing, they were fixed and stained with the methods used for blood smears. Results showed that not all fixing solutions and stains were an equally good choice for cultured lymphocytes.
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spelling Light microscope observation of circulating human lymphocytes cultured in vitrobloodin vitrolymphocytesmicroscopymorphologyPercollThe purpose of this work was to study the isolation and a light microscopy technique for cultured lymphocytes. Blood samples were obtained by venipuncture with an anticoagulant added and centrifuged in a Percoll density gradient to separate the leukocytes. Lymphocytes were placed in 25 cm ³ tissue culture flasks at 37ºC. After culturing, they were fixed and stained with the methods used for blood smears. Results showed that not all fixing solutions and stains were an equally good choice for cultured lymphocytes.Instituto de Tecnologia do Paraná - Tecpar2010-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500013Brazilian Archives of Biology and Technology v.53 n.5 2010reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132010000500013info:eu-repo/semantics/openAccessOliveira,Naila Francis Paulo deDolder,Mary Anne HeidiGenari,Selma Candeláriaeng2010-11-03T00:00:00Zoai:scielo:S1516-89132010000500013Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2010-11-03T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Light microscope observation of circulating human lymphocytes cultured in vitro
title Light microscope observation of circulating human lymphocytes cultured in vitro
spellingShingle Light microscope observation of circulating human lymphocytes cultured in vitro
Oliveira,Naila Francis Paulo de
blood
in vitro
lymphocytes
microscopy
morphology
Percoll
title_short Light microscope observation of circulating human lymphocytes cultured in vitro
title_full Light microscope observation of circulating human lymphocytes cultured in vitro
title_fullStr Light microscope observation of circulating human lymphocytes cultured in vitro
title_full_unstemmed Light microscope observation of circulating human lymphocytes cultured in vitro
title_sort Light microscope observation of circulating human lymphocytes cultured in vitro
author Oliveira,Naila Francis Paulo de
author_facet Oliveira,Naila Francis Paulo de
Dolder,Mary Anne Heidi
Genari,Selma Candelária
author_role author
author2 Dolder,Mary Anne Heidi
Genari,Selma Candelária
author2_role author
author
dc.contributor.author.fl_str_mv Oliveira,Naila Francis Paulo de
Dolder,Mary Anne Heidi
Genari,Selma Candelária
dc.subject.por.fl_str_mv blood
in vitro
lymphocytes
microscopy
morphology
Percoll
topic blood
in vitro
lymphocytes
microscopy
morphology
Percoll
description The purpose of this work was to study the isolation and a light microscopy technique for cultured lymphocytes. Blood samples were obtained by venipuncture with an anticoagulant added and centrifuged in a Percoll density gradient to separate the leukocytes. Lymphocytes were placed in 25 cm ³ tissue culture flasks at 37ºC. After culturing, they were fixed and stained with the methods used for blood smears. Results showed that not all fixing solutions and stains were an equally good choice for cultured lymphocytes.
publishDate 2010
dc.date.none.fl_str_mv 2010-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500013
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500013
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132010000500013
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.53 n.5 2010
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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