Anticandidal Activity of Hydroalcoholic Extract of Phyllanthus niruri L. (Stone-Breaker)
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132022000100322 |
Resumo: | Abstract Candida is becoming more resistant to conventional treatments, and causes persistent and severe infections. This study evaluates the antifungal and virulence activities of the hydroalcoholic extract of Phyllanthus niruri (HE-Pn) on Candida. HE-Pn was prepared by maceration technique. Chemical composition of HE-Pn was determined using Gas Chromatography-Mass Spectrometry (GS-MS). Antifungal screening was done using agar well diffusion. CLSI M27-A3 was used to determine the Minimum Inhibitory (MIC) and Fungicidal Concentrations (MFC). Effects of HE-Pn on adhesion and germ tube of C. albicans (ATCC MYA-2876) were determined using XTT assay and germ tube formation assay, respectively. Transmission Electron Microscopy (TEM) was performed to visualize the post-exposure cellular changes. HE-Pn cytotoxicity was determined using human keratinocyte cell line (HaCaT). Chlorhexidine digluconate (2 mg/mL) was used as the positive control. Linolenic acid ethyl ester was the most abundant chemical component of HE-Pn. All strains tested were sensitive to HE-Pn. MIC were 0.03 - 8 mg/mL and MFC were 0.5 - 64 mg/mL for all test strains. C. albicans (ATCC MYA-2876) showed 50% of adhesion reduction with > 4 mg/mL of HE-Pn and germ-tube formation was inhibited with 0.25 and 2 mg/mL. TEM exhibited cytoplasmic granulation, intracellular vacuoles, detachment of cell wall and plasma membrane and chromatin condensation of Candida. No toxicity of HE-Pn was noted on HaCaT cells. HE-Pn shows an anti-Candida activity and can be used as an inhibitory agent against adhesion and germ tube formation of Candida albicans (ATCC MYA-2876) without causing any toxicity to human cells. |
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Anticandidal Activity of Hydroalcoholic Extract of Phyllanthus niruri L. (Stone-Breaker)Phyllanthus nirurihydroalcoholicplant extractCandida albicansHaCaT cellsantifungal agentAbstract Candida is becoming more resistant to conventional treatments, and causes persistent and severe infections. This study evaluates the antifungal and virulence activities of the hydroalcoholic extract of Phyllanthus niruri (HE-Pn) on Candida. HE-Pn was prepared by maceration technique. Chemical composition of HE-Pn was determined using Gas Chromatography-Mass Spectrometry (GS-MS). Antifungal screening was done using agar well diffusion. CLSI M27-A3 was used to determine the Minimum Inhibitory (MIC) and Fungicidal Concentrations (MFC). Effects of HE-Pn on adhesion and germ tube of C. albicans (ATCC MYA-2876) were determined using XTT assay and germ tube formation assay, respectively. Transmission Electron Microscopy (TEM) was performed to visualize the post-exposure cellular changes. HE-Pn cytotoxicity was determined using human keratinocyte cell line (HaCaT). Chlorhexidine digluconate (2 mg/mL) was used as the positive control. Linolenic acid ethyl ester was the most abundant chemical component of HE-Pn. All strains tested were sensitive to HE-Pn. MIC were 0.03 - 8 mg/mL and MFC were 0.5 - 64 mg/mL for all test strains. C. albicans (ATCC MYA-2876) showed 50% of adhesion reduction with > 4 mg/mL of HE-Pn and germ-tube formation was inhibited with 0.25 and 2 mg/mL. TEM exhibited cytoplasmic granulation, intracellular vacuoles, detachment of cell wall and plasma membrane and chromatin condensation of Candida. No toxicity of HE-Pn was noted on HaCaT cells. HE-Pn shows an anti-Candida activity and can be used as an inhibitory agent against adhesion and germ tube formation of Candida albicans (ATCC MYA-2876) without causing any toxicity to human cells.Instituto de Tecnologia do Paraná - Tecpar2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132022000100322Brazilian Archives of Biology and Technology v.65 2022reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/1678-4324-2022210539info:eu-repo/semantics/openAccessMaia,Flávia CamilaWijesinghe,Gayan KanchanaBarbosa,Janaína PriscilaFeiria,Simone Nataly Busato deOliveira,Thais RossiniBoni,Giovana ClaudiaJóia,FelipeCardoso,Vanessa da SilvaFranco,Valéria Alessandra Prado DefávariAnibal,Paula CristinaHöfling,José Franciscoeng2022-06-23T00:00:00Zoai:scielo:S1516-89132022000100322Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2022-06-23T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Anticandidal Activity of Hydroalcoholic Extract of Phyllanthus niruri L. (Stone-Breaker) |
title |
Anticandidal Activity of Hydroalcoholic Extract of Phyllanthus niruri L. (Stone-Breaker) |
spellingShingle |
Anticandidal Activity of Hydroalcoholic Extract of Phyllanthus niruri L. (Stone-Breaker) Maia,Flávia Camila Phyllanthus niruri hydroalcoholic plant extract Candida albicans HaCaT cells antifungal agent |
title_short |
Anticandidal Activity of Hydroalcoholic Extract of Phyllanthus niruri L. (Stone-Breaker) |
title_full |
Anticandidal Activity of Hydroalcoholic Extract of Phyllanthus niruri L. (Stone-Breaker) |
title_fullStr |
Anticandidal Activity of Hydroalcoholic Extract of Phyllanthus niruri L. (Stone-Breaker) |
title_full_unstemmed |
Anticandidal Activity of Hydroalcoholic Extract of Phyllanthus niruri L. (Stone-Breaker) |
title_sort |
Anticandidal Activity of Hydroalcoholic Extract of Phyllanthus niruri L. (Stone-Breaker) |
author |
Maia,Flávia Camila |
author_facet |
Maia,Flávia Camila Wijesinghe,Gayan Kanchana Barbosa,Janaína Priscila Feiria,Simone Nataly Busato de Oliveira,Thais Rossini Boni,Giovana Claudia Jóia,Felipe Cardoso,Vanessa da Silva Franco,Valéria Alessandra Prado Defávari Anibal,Paula Cristina Höfling,José Francisco |
author_role |
author |
author2 |
Wijesinghe,Gayan Kanchana Barbosa,Janaína Priscila Feiria,Simone Nataly Busato de Oliveira,Thais Rossini Boni,Giovana Claudia Jóia,Felipe Cardoso,Vanessa da Silva Franco,Valéria Alessandra Prado Defávari Anibal,Paula Cristina Höfling,José Francisco |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Maia,Flávia Camila Wijesinghe,Gayan Kanchana Barbosa,Janaína Priscila Feiria,Simone Nataly Busato de Oliveira,Thais Rossini Boni,Giovana Claudia Jóia,Felipe Cardoso,Vanessa da Silva Franco,Valéria Alessandra Prado Defávari Anibal,Paula Cristina Höfling,José Francisco |
dc.subject.por.fl_str_mv |
Phyllanthus niruri hydroalcoholic plant extract Candida albicans HaCaT cells antifungal agent |
topic |
Phyllanthus niruri hydroalcoholic plant extract Candida albicans HaCaT cells antifungal agent |
description |
Abstract Candida is becoming more resistant to conventional treatments, and causes persistent and severe infections. This study evaluates the antifungal and virulence activities of the hydroalcoholic extract of Phyllanthus niruri (HE-Pn) on Candida. HE-Pn was prepared by maceration technique. Chemical composition of HE-Pn was determined using Gas Chromatography-Mass Spectrometry (GS-MS). Antifungal screening was done using agar well diffusion. CLSI M27-A3 was used to determine the Minimum Inhibitory (MIC) and Fungicidal Concentrations (MFC). Effects of HE-Pn on adhesion and germ tube of C. albicans (ATCC MYA-2876) were determined using XTT assay and germ tube formation assay, respectively. Transmission Electron Microscopy (TEM) was performed to visualize the post-exposure cellular changes. HE-Pn cytotoxicity was determined using human keratinocyte cell line (HaCaT). Chlorhexidine digluconate (2 mg/mL) was used as the positive control. Linolenic acid ethyl ester was the most abundant chemical component of HE-Pn. All strains tested were sensitive to HE-Pn. MIC were 0.03 - 8 mg/mL and MFC were 0.5 - 64 mg/mL for all test strains. C. albicans (ATCC MYA-2876) showed 50% of adhesion reduction with > 4 mg/mL of HE-Pn and germ-tube formation was inhibited with 0.25 and 2 mg/mL. TEM exhibited cytoplasmic granulation, intracellular vacuoles, detachment of cell wall and plasma membrane and chromatin condensation of Candida. No toxicity of HE-Pn was noted on HaCaT cells. HE-Pn shows an anti-Candida activity and can be used as an inhibitory agent against adhesion and germ tube formation of Candida albicans (ATCC MYA-2876) without causing any toxicity to human cells. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132022000100322 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132022000100322 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-4324-2022210539 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.65 2022 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318281376399360 |