Methodological variations in the isolation of genomic DNA from Streptococcus bacteria

Detalhes bibliográficos
Autor(a) principal: Moreira,Mônica
Data de Publicação: 2010
Outros Autores: Noschang,Juliana, Neiva,Ivana Froede, Carvalho,Yanê, Higuti,llma Hiroko, Vicente,Vânia Aparecida
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400013
Resumo: In this work, genomic DNA of Streptococcus pyogenes, S. mutans and S. sobrinus was isolated using two methods: either using the detergent cetyltrimethylammonium bromide (CTAB) at 65ºC; or by applying ultrasound to a mixture of silica and celite in CTAB. The composite method that used ultrasound was the more efficient, allowing the straightforward extraction of genomic DNA from Gram-positive bacteria with good quality and reproducibility.
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spelling Methodological variations in the isolation of genomic DNA from Streptococcus bacteriaStreptococcusDNA isolationCTABultrasoundIn this work, genomic DNA of Streptococcus pyogenes, S. mutans and S. sobrinus was isolated using two methods: either using the detergent cetyltrimethylammonium bromide (CTAB) at 65ºC; or by applying ultrasound to a mixture of silica and celite in CTAB. The composite method that used ultrasound was the more efficient, allowing the straightforward extraction of genomic DNA from Gram-positive bacteria with good quality and reproducibility.Instituto de Tecnologia do Paraná - Tecpar2010-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400013Brazilian Archives of Biology and Technology v.53 n.4 2010reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132010000400013info:eu-repo/semantics/openAccessMoreira,MônicaNoschang,JulianaNeiva,Ivana FroedeCarvalho,YanêHiguti,llma HirokoVicente,Vânia Aparecidaeng2010-08-06T00:00:00Zoai:scielo:S1516-89132010000400013Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2010-08-06T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Methodological variations in the isolation of genomic DNA from Streptococcus bacteria
title Methodological variations in the isolation of genomic DNA from Streptococcus bacteria
spellingShingle Methodological variations in the isolation of genomic DNA from Streptococcus bacteria
Moreira,Mônica
Streptococcus
DNA isolation
CTAB
ultrasound
title_short Methodological variations in the isolation of genomic DNA from Streptococcus bacteria
title_full Methodological variations in the isolation of genomic DNA from Streptococcus bacteria
title_fullStr Methodological variations in the isolation of genomic DNA from Streptococcus bacteria
title_full_unstemmed Methodological variations in the isolation of genomic DNA from Streptococcus bacteria
title_sort Methodological variations in the isolation of genomic DNA from Streptococcus bacteria
author Moreira,Mônica
author_facet Moreira,Mônica
Noschang,Juliana
Neiva,Ivana Froede
Carvalho,Yanê
Higuti,llma Hiroko
Vicente,Vânia Aparecida
author_role author
author2 Noschang,Juliana
Neiva,Ivana Froede
Carvalho,Yanê
Higuti,llma Hiroko
Vicente,Vânia Aparecida
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Moreira,Mônica
Noschang,Juliana
Neiva,Ivana Froede
Carvalho,Yanê
Higuti,llma Hiroko
Vicente,Vânia Aparecida
dc.subject.por.fl_str_mv Streptococcus
DNA isolation
CTAB
ultrasound
topic Streptococcus
DNA isolation
CTAB
ultrasound
description In this work, genomic DNA of Streptococcus pyogenes, S. mutans and S. sobrinus was isolated using two methods: either using the detergent cetyltrimethylammonium bromide (CTAB) at 65ºC; or by applying ultrasound to a mixture of silica and celite in CTAB. The composite method that used ultrasound was the more efficient, allowing the straightforward extraction of genomic DNA from Gram-positive bacteria with good quality and reproducibility.
publishDate 2010
dc.date.none.fl_str_mv 2010-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400013
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400013
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132010000400013
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.53 n.4 2010
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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