PROTOCOL SUGGESTED FOR DNA EXTRACTION OF JABUTICABA TREES

Detalhes bibliográficos
Autor(a) principal: Danner, Moeses Andrigo
Data de Publicação: 2011
Outros Autores: Sasso, Simone Aparecida Zolet, Bittencourt, Juliana Vitória Messias, Citadin, Idemir, Sachet, Marcos Robson
Tipo de documento: Artigo
Idioma: por
Título da fonte: Ciência Florestal (Online)
Texto Completo: https://periodicos.ufsm.br/cienciaflorestal/article/view/3241
Resumo: This study aimed to adapt the protocol described by Soto (2001), to test different sources (plant tissue) and to establish conditions for collection and pre-treatment of samples for extracting DNA from jabuticaba trees (Plinia cauliflora (DC.) Berg, Myrtaceae). For this purpose, developing and expanded leaves, cambium of the stem and leaf stalk were used. The protocol tested can be used for DNA extraction from jabuticaba trees, because it provided DNA isolation of good quality and allowed the amplification of DNA through of PCR (Polymerase Chain Reaction). The use of liquid nitrogen for the maceration of leaves is necessary to prevent oxidation.
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spelling PROTOCOL SUGGESTED FOR DNA EXTRACTION OF JABUTICABA TREESProposta de protocolo para extração de DNA de JabuticabeiraPlinia caulifloraDNA isolationCTAB methodPlinia caulifloraisolamento de DNAmétodo CTABThis study aimed to adapt the protocol described by Soto (2001), to test different sources (plant tissue) and to establish conditions for collection and pre-treatment of samples for extracting DNA from jabuticaba trees (Plinia cauliflora (DC.) Berg, Myrtaceae). For this purpose, developing and expanded leaves, cambium of the stem and leaf stalk were used. The protocol tested can be used for DNA extraction from jabuticaba trees, because it provided DNA isolation of good quality and allowed the amplification of DNA through of PCR (Polymerase Chain Reaction). The use of liquid nitrogen for the maceration of leaves is necessary to prevent oxidation.O presente trabalho teve por objetivo adaptar o protocolo descrito por Soto (2001), testar diferentes fontes (tecido vegetal) e estabelecer condições de coleta e pré-tratamento de amostras para extração de DNA de jabuticabeira (Plinia cauliflora (DC.) Berg, Myrtaceae). Utilizaram-se folhas em desenvolvimento, folhas completamente expandidas, câmbio do caule e pecíolo foliar. O protocolo testado pode ser utilizado para extração de DNA de jabuticabeira, pois proporcionou isolamento de DNA de boa qualidade e possibilitou amplificação por meio de PCR ("Reação de Polimerase em Cadeia"). Para evitar a oxidação de folhas é necessário realizar a maceração com nitrogênio líquido.Universidade Federal de Santa Maria2011-06-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufsm.br/cienciaflorestal/article/view/324110.5902/198050983241Ciência Florestal; Vol. 21 No. 2 (2011); 363-367Ciência Florestal; v. 21 n. 2 (2011); 363-3671980-50980103-9954reponame:Ciência Florestal (Online)instname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMporhttps://periodicos.ufsm.br/cienciaflorestal/article/view/3241/1882Danner, Moeses AndrigoSasso, Simone Aparecida ZoletBittencourt, Juliana Vitória MessiasCitadin, IdemirSachet, Marcos Robsoninfo:eu-repo/semantics/openAccess2017-05-04T11:03:29Zoai:ojs.pkp.sfu.ca:article/3241Revistahttp://www.ufsm.br/cienciaflorestal/ONGhttps://old.scielo.br/oai/scielo-oai.php||cienciaflorestal@ufsm.br|| cienciaflorestal@gmail.com|| cf@smail.ufsm.br1980-50980103-9954opendoar:2017-05-04T11:03:29Ciência Florestal (Online) - Universidade Federal de Santa Maria (UFSM)false
dc.title.none.fl_str_mv PROTOCOL SUGGESTED FOR DNA EXTRACTION OF JABUTICABA TREES
Proposta de protocolo para extração de DNA de Jabuticabeira
title PROTOCOL SUGGESTED FOR DNA EXTRACTION OF JABUTICABA TREES
spellingShingle PROTOCOL SUGGESTED FOR DNA EXTRACTION OF JABUTICABA TREES
Danner, Moeses Andrigo
Plinia cauliflora
DNA isolation
CTAB method
Plinia cauliflora
isolamento de DNA
método CTAB
title_short PROTOCOL SUGGESTED FOR DNA EXTRACTION OF JABUTICABA TREES
title_full PROTOCOL SUGGESTED FOR DNA EXTRACTION OF JABUTICABA TREES
title_fullStr PROTOCOL SUGGESTED FOR DNA EXTRACTION OF JABUTICABA TREES
title_full_unstemmed PROTOCOL SUGGESTED FOR DNA EXTRACTION OF JABUTICABA TREES
title_sort PROTOCOL SUGGESTED FOR DNA EXTRACTION OF JABUTICABA TREES
author Danner, Moeses Andrigo
author_facet Danner, Moeses Andrigo
Sasso, Simone Aparecida Zolet
Bittencourt, Juliana Vitória Messias
Citadin, Idemir
Sachet, Marcos Robson
author_role author
author2 Sasso, Simone Aparecida Zolet
Bittencourt, Juliana Vitória Messias
Citadin, Idemir
Sachet, Marcos Robson
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Danner, Moeses Andrigo
Sasso, Simone Aparecida Zolet
Bittencourt, Juliana Vitória Messias
Citadin, Idemir
Sachet, Marcos Robson
dc.subject.por.fl_str_mv Plinia cauliflora
DNA isolation
CTAB method
Plinia cauliflora
isolamento de DNA
método CTAB
topic Plinia cauliflora
DNA isolation
CTAB method
Plinia cauliflora
isolamento de DNA
método CTAB
description This study aimed to adapt the protocol described by Soto (2001), to test different sources (plant tissue) and to establish conditions for collection and pre-treatment of samples for extracting DNA from jabuticaba trees (Plinia cauliflora (DC.) Berg, Myrtaceae). For this purpose, developing and expanded leaves, cambium of the stem and leaf stalk were used. The protocol tested can be used for DNA extraction from jabuticaba trees, because it provided DNA isolation of good quality and allowed the amplification of DNA through of PCR (Polymerase Chain Reaction). The use of liquid nitrogen for the maceration of leaves is necessary to prevent oxidation.
publishDate 2011
dc.date.none.fl_str_mv 2011-06-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.ufsm.br/cienciaflorestal/article/view/3241
10.5902/198050983241
url https://periodicos.ufsm.br/cienciaflorestal/article/view/3241
identifier_str_mv 10.5902/198050983241
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://periodicos.ufsm.br/cienciaflorestal/article/view/3241/1882
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv Ciência Florestal; Vol. 21 No. 2 (2011); 363-367
Ciência Florestal; v. 21 n. 2 (2011); 363-367
1980-5098
0103-9954
reponame:Ciência Florestal (Online)
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Ciência Florestal (Online)
collection Ciência Florestal (Online)
repository.name.fl_str_mv Ciência Florestal (Online) - Universidade Federal de Santa Maria (UFSM)
repository.mail.fl_str_mv ||cienciaflorestal@ufsm.br|| cienciaflorestal@gmail.com|| cf@smail.ufsm.br
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