The Mutagenesis of Lactobacillus Thermophilus for Enhanced L-(+)-Lactic Acid Accumulation Induced by Heavy Ion Irradiation
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100504 |
Resumo: | ABSTRACT Screening promising L. thermophiles with high productivity, high efficiency and strong adaptability are very important in lactic acid industry. For this purpose, 80MeV/u carbon ions were applied to irradiate L. thermophiles. After high-throughput screening, a mutant, named SRZ50, was obtained. Different carbon sources or nitrogen sources were provided to investigate carbon or nitrogen source utilization between mutant SRZ50 and wild type, and different fermentation periods were also chose to study fermentation characteristic between mutant SRZ50 and wild type. The results showed that mutant SRZ50 exhibited the enhanced L-(+)-lactic acid production from wild type. When glucose or fructose was the sole carbon source, the L(+)-lactic acid production by mutant SRZ50 was both the highest, respectively, 23.16 ± 0.72 g/L or 23.24 ± 0.66 g/L, which had a significant increase from that of wild type (P<0.01), following obvious increase in biomass (P<0.05). When yeast powder was the sole nitrogen source, it can promote mutant SRZ50 to accumulate the highest L-(+)-lactic acid accumulation, which also had a significant increase from that of wild type (P<0.01). Under different fermentation periods, it was obtained that mutant SRZ50 all exhibited significant increase in L-(+)-lactic acid accumulation from wild type. In conclusion, a mutant strain with improved production profiles for L-(+)-lactic acid, was obtained, indicating that heavy ions can be an efficient tool to improve metabolic product accumulations in microbes. |
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Brazilian Archives of Biology and Technology |
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The Mutagenesis of Lactobacillus Thermophilus for Enhanced L-(+)-Lactic Acid Accumulation Induced by Heavy Ion IrradiationL. thermophilusmutantheavy ion irradiationL-(+)-lactic acid productioncarbon sourcefermentation characteristicABSTRACT Screening promising L. thermophiles with high productivity, high efficiency and strong adaptability are very important in lactic acid industry. For this purpose, 80MeV/u carbon ions were applied to irradiate L. thermophiles. After high-throughput screening, a mutant, named SRZ50, was obtained. Different carbon sources or nitrogen sources were provided to investigate carbon or nitrogen source utilization between mutant SRZ50 and wild type, and different fermentation periods were also chose to study fermentation characteristic between mutant SRZ50 and wild type. The results showed that mutant SRZ50 exhibited the enhanced L-(+)-lactic acid production from wild type. When glucose or fructose was the sole carbon source, the L(+)-lactic acid production by mutant SRZ50 was both the highest, respectively, 23.16 ± 0.72 g/L or 23.24 ± 0.66 g/L, which had a significant increase from that of wild type (P<0.01), following obvious increase in biomass (P<0.05). When yeast powder was the sole nitrogen source, it can promote mutant SRZ50 to accumulate the highest L-(+)-lactic acid accumulation, which also had a significant increase from that of wild type (P<0.01). Under different fermentation periods, it was obtained that mutant SRZ50 all exhibited significant increase in L-(+)-lactic acid accumulation from wild type. In conclusion, a mutant strain with improved production profiles for L-(+)-lactic acid, was obtained, indicating that heavy ions can be an efficient tool to improve metabolic product accumulations in microbes.Instituto de Tecnologia do Paraná - Tecpar2017-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100504Brazilian Archives of Biology and Technology v.60 2017reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/1678-4324-2016160337info:eu-repo/semantics/openAccessHu,WeiChen,JihongWu,QinghuaLi,WenjianLiu,JingLu,DongWang,Shuyangeng2018-12-03T00:00:00Zoai:scielo:S1516-89132017000100504Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2018-12-03T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
The Mutagenesis of Lactobacillus Thermophilus for Enhanced L-(+)-Lactic Acid Accumulation Induced by Heavy Ion Irradiation |
title |
The Mutagenesis of Lactobacillus Thermophilus for Enhanced L-(+)-Lactic Acid Accumulation Induced by Heavy Ion Irradiation |
spellingShingle |
The Mutagenesis of Lactobacillus Thermophilus for Enhanced L-(+)-Lactic Acid Accumulation Induced by Heavy Ion Irradiation Hu,Wei L. thermophilus mutant heavy ion irradiation L-(+)-lactic acid production carbon source fermentation characteristic |
title_short |
The Mutagenesis of Lactobacillus Thermophilus for Enhanced L-(+)-Lactic Acid Accumulation Induced by Heavy Ion Irradiation |
title_full |
The Mutagenesis of Lactobacillus Thermophilus for Enhanced L-(+)-Lactic Acid Accumulation Induced by Heavy Ion Irradiation |
title_fullStr |
The Mutagenesis of Lactobacillus Thermophilus for Enhanced L-(+)-Lactic Acid Accumulation Induced by Heavy Ion Irradiation |
title_full_unstemmed |
The Mutagenesis of Lactobacillus Thermophilus for Enhanced L-(+)-Lactic Acid Accumulation Induced by Heavy Ion Irradiation |
title_sort |
The Mutagenesis of Lactobacillus Thermophilus for Enhanced L-(+)-Lactic Acid Accumulation Induced by Heavy Ion Irradiation |
author |
Hu,Wei |
author_facet |
Hu,Wei Chen,Jihong Wu,Qinghua Li,Wenjian Liu,Jing Lu,Dong Wang,Shuyang |
author_role |
author |
author2 |
Chen,Jihong Wu,Qinghua Li,Wenjian Liu,Jing Lu,Dong Wang,Shuyang |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Hu,Wei Chen,Jihong Wu,Qinghua Li,Wenjian Liu,Jing Lu,Dong Wang,Shuyang |
dc.subject.por.fl_str_mv |
L. thermophilus mutant heavy ion irradiation L-(+)-lactic acid production carbon source fermentation characteristic |
topic |
L. thermophilus mutant heavy ion irradiation L-(+)-lactic acid production carbon source fermentation characteristic |
description |
ABSTRACT Screening promising L. thermophiles with high productivity, high efficiency and strong adaptability are very important in lactic acid industry. For this purpose, 80MeV/u carbon ions were applied to irradiate L. thermophiles. After high-throughput screening, a mutant, named SRZ50, was obtained. Different carbon sources or nitrogen sources were provided to investigate carbon or nitrogen source utilization between mutant SRZ50 and wild type, and different fermentation periods were also chose to study fermentation characteristic between mutant SRZ50 and wild type. The results showed that mutant SRZ50 exhibited the enhanced L-(+)-lactic acid production from wild type. When glucose or fructose was the sole carbon source, the L(+)-lactic acid production by mutant SRZ50 was both the highest, respectively, 23.16 ± 0.72 g/L or 23.24 ± 0.66 g/L, which had a significant increase from that of wild type (P<0.01), following obvious increase in biomass (P<0.05). When yeast powder was the sole nitrogen source, it can promote mutant SRZ50 to accumulate the highest L-(+)-lactic acid accumulation, which also had a significant increase from that of wild type (P<0.01). Under different fermentation periods, it was obtained that mutant SRZ50 all exhibited significant increase in L-(+)-lactic acid accumulation from wild type. In conclusion, a mutant strain with improved production profiles for L-(+)-lactic acid, was obtained, indicating that heavy ions can be an efficient tool to improve metabolic product accumulations in microbes. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100504 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100504 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-4324-2016160337 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.60 2017 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
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1750318278226477056 |