Modeling gallic acid production rate by empirical and statistical analysis

Detalhes bibliográficos
Autor(a) principal: Kar,Bratati
Data de Publicação: 2000
Outros Autores: Banerjee,Rintu, Bhattacharyya,Bimal Chandra
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000500010
Resumo: For predicting the rate of enzymatic reaction empirical correlation based on the experimental results obtained under various operating conditions have been developed. Models represent both the activation as well as deactivation conditions of enzymatic hydrolysis and the results have been analyzed by analysis of variance (ANOVA). The tannase activity was found maximum at incubation time 5 min, reaction temperature 40ºC, pH 4.0, initial enzyme concentration 0.12 v/v, initial substrate concentration 0.42 mg/ml, ionic strength 0.2 M and under these optimal conditions, the maximum rate of gallic acid production was 33.49 mumoles/ml/min.
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spelling Modeling gallic acid production rate by empirical and statistical analysisGallic acidmodelingtannaseCaesalpinia digynaFor predicting the rate of enzymatic reaction empirical correlation based on the experimental results obtained under various operating conditions have been developed. Models represent both the activation as well as deactivation conditions of enzymatic hydrolysis and the results have been analyzed by analysis of variance (ANOVA). The tannase activity was found maximum at incubation time 5 min, reaction temperature 40ºC, pH 4.0, initial enzyme concentration 0.12 v/v, initial substrate concentration 0.42 mg/ml, ionic strength 0.2 M and under these optimal conditions, the maximum rate of gallic acid production was 33.49 mumoles/ml/min.Instituto de Tecnologia do Paraná - Tecpar2000-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000500010Brazilian Archives of Biology and Technology v.43 n.5 2000reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132000000500010info:eu-repo/semantics/openAccessKar,BratatiBanerjee,RintuBhattacharyya,Bimal Chandraeng2001-10-25T00:00:00Zoai:scielo:S1516-89132000000500010Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2001-10-25T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Modeling gallic acid production rate by empirical and statistical analysis
title Modeling gallic acid production rate by empirical and statistical analysis
spellingShingle Modeling gallic acid production rate by empirical and statistical analysis
Kar,Bratati
Gallic acid
modeling
tannase
Caesalpinia digyna
title_short Modeling gallic acid production rate by empirical and statistical analysis
title_full Modeling gallic acid production rate by empirical and statistical analysis
title_fullStr Modeling gallic acid production rate by empirical and statistical analysis
title_full_unstemmed Modeling gallic acid production rate by empirical and statistical analysis
title_sort Modeling gallic acid production rate by empirical and statistical analysis
author Kar,Bratati
author_facet Kar,Bratati
Banerjee,Rintu
Bhattacharyya,Bimal Chandra
author_role author
author2 Banerjee,Rintu
Bhattacharyya,Bimal Chandra
author2_role author
author
dc.contributor.author.fl_str_mv Kar,Bratati
Banerjee,Rintu
Bhattacharyya,Bimal Chandra
dc.subject.por.fl_str_mv Gallic acid
modeling
tannase
Caesalpinia digyna
topic Gallic acid
modeling
tannase
Caesalpinia digyna
description For predicting the rate of enzymatic reaction empirical correlation based on the experimental results obtained under various operating conditions have been developed. Models represent both the activation as well as deactivation conditions of enzymatic hydrolysis and the results have been analyzed by analysis of variance (ANOVA). The tannase activity was found maximum at incubation time 5 min, reaction temperature 40ºC, pH 4.0, initial enzyme concentration 0.12 v/v, initial substrate concentration 0.42 mg/ml, ionic strength 0.2 M and under these optimal conditions, the maximum rate of gallic acid production was 33.49 mumoles/ml/min.
publishDate 2000
dc.date.none.fl_str_mv 2000-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000500010
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000500010
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132000000500010
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.43 n.5 2000
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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