A comparative study on fungal laccases immobilized on chitosan
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400001 |
Resumo: | The phenoloxidase enzyme laccase from the cultures of the Pleurotus ostreatus and Botryosphaeria sp. and a commercial laccase from Aspergillus sp. were immobilized on chitosan of pharmaceutical degree by adsorption followed by crosslinking. Different immobilization conditions in relation to the granulometry of support and amount of enzymatic laccase extract used were tested, aiming at reaching high enzymatic activity with the immobilized enzyme. Two different substrates, ABTS and DMP, were used for the determination of enzymatic activity. The highest enzymatic activity was obtained when 1.0mg/mL of the enzymatic laccase extract from Botryosphaeria sp. was used with 1.0g of support (200 mesh). These immobilized enzymes are to be applied to the improvement of white wines by the degradation of undesirable phenolic compounds. |
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Brazilian Archives of Biology and Technology |
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A comparative study on fungal laccases immobilized on chitosanLaccasesimmobilizationchitosanThe phenoloxidase enzyme laccase from the cultures of the Pleurotus ostreatus and Botryosphaeria sp. and a commercial laccase from Aspergillus sp. were immobilized on chitosan of pharmaceutical degree by adsorption followed by crosslinking. Different immobilization conditions in relation to the granulometry of support and amount of enzymatic laccase extract used were tested, aiming at reaching high enzymatic activity with the immobilized enzyme. Two different substrates, ABTS and DMP, were used for the determination of enzymatic activity. The highest enzymatic activity was obtained when 1.0mg/mL of the enzymatic laccase extract from Botryosphaeria sp. was used with 1.0g of support (200 mesh). These immobilized enzymes are to be applied to the improvement of white wines by the degradation of undesirable phenolic compounds.Instituto de Tecnologia do Paraná - Tecpar2005-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400001Brazilian Archives of Biology and Technology v.48 n.spe 2005reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132005000400001info:eu-repo/semantics/openAccessAraújo,José Hilton Bernardino deUemura,Vinicius OliveiraMoraes,Flavio Faria deBarbosa,Aneli de MeloZanin,Gisella Mariaeng2005-08-15T00:00:00Zoai:scielo:S1516-89132005000400001Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2005-08-15T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
A comparative study on fungal laccases immobilized on chitosan |
title |
A comparative study on fungal laccases immobilized on chitosan |
spellingShingle |
A comparative study on fungal laccases immobilized on chitosan Araújo,José Hilton Bernardino de Laccases immobilization chitosan |
title_short |
A comparative study on fungal laccases immobilized on chitosan |
title_full |
A comparative study on fungal laccases immobilized on chitosan |
title_fullStr |
A comparative study on fungal laccases immobilized on chitosan |
title_full_unstemmed |
A comparative study on fungal laccases immobilized on chitosan |
title_sort |
A comparative study on fungal laccases immobilized on chitosan |
author |
Araújo,José Hilton Bernardino de |
author_facet |
Araújo,José Hilton Bernardino de Uemura,Vinicius Oliveira Moraes,Flavio Faria de Barbosa,Aneli de Melo Zanin,Gisella Maria |
author_role |
author |
author2 |
Uemura,Vinicius Oliveira Moraes,Flavio Faria de Barbosa,Aneli de Melo Zanin,Gisella Maria |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Araújo,José Hilton Bernardino de Uemura,Vinicius Oliveira Moraes,Flavio Faria de Barbosa,Aneli de Melo Zanin,Gisella Maria |
dc.subject.por.fl_str_mv |
Laccases immobilization chitosan |
topic |
Laccases immobilization chitosan |
description |
The phenoloxidase enzyme laccase from the cultures of the Pleurotus ostreatus and Botryosphaeria sp. and a commercial laccase from Aspergillus sp. were immobilized on chitosan of pharmaceutical degree by adsorption followed by crosslinking. Different immobilization conditions in relation to the granulometry of support and amount of enzymatic laccase extract used were tested, aiming at reaching high enzymatic activity with the immobilized enzyme. Two different substrates, ABTS and DMP, were used for the determination of enzymatic activity. The highest enzymatic activity was obtained when 1.0mg/mL of the enzymatic laccase extract from Botryosphaeria sp. was used with 1.0g of support (200 mesh). These immobilized enzymes are to be applied to the improvement of white wines by the degradation of undesirable phenolic compounds. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400001 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-89132005000400001 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.48 n.spe 2005 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318270287708160 |