A comparative study on fungal laccases immobilized on chitosan

Detalhes bibliográficos
Autor(a) principal: Araújo,José Hilton Bernardino de
Data de Publicação: 2005
Outros Autores: Uemura,Vinicius Oliveira, Moraes,Flavio Faria de, Barbosa,Aneli de Melo, Zanin,Gisella Maria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400001
Resumo: The phenoloxidase enzyme laccase from the cultures of the Pleurotus ostreatus and Botryosphaeria sp. and a commercial laccase from Aspergillus sp. were immobilized on chitosan of pharmaceutical degree by adsorption followed by crosslinking. Different immobilization conditions in relation to the granulometry of support and amount of enzymatic laccase extract used were tested, aiming at reaching high enzymatic activity with the immobilized enzyme. Two different substrates, ABTS and DMP, were used for the determination of enzymatic activity. The highest enzymatic activity was obtained when 1.0mg/mL of the enzymatic laccase extract from Botryosphaeria sp. was used with 1.0g of support (200 mesh). These immobilized enzymes are to be applied to the improvement of white wines by the degradation of undesirable phenolic compounds.
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spelling A comparative study on fungal laccases immobilized on chitosanLaccasesimmobilizationchitosanThe phenoloxidase enzyme laccase from the cultures of the Pleurotus ostreatus and Botryosphaeria sp. and a commercial laccase from Aspergillus sp. were immobilized on chitosan of pharmaceutical degree by adsorption followed by crosslinking. Different immobilization conditions in relation to the granulometry of support and amount of enzymatic laccase extract used were tested, aiming at reaching high enzymatic activity with the immobilized enzyme. Two different substrates, ABTS and DMP, were used for the determination of enzymatic activity. The highest enzymatic activity was obtained when 1.0mg/mL of the enzymatic laccase extract from Botryosphaeria sp. was used with 1.0g of support (200 mesh). These immobilized enzymes are to be applied to the improvement of white wines by the degradation of undesirable phenolic compounds.Instituto de Tecnologia do Paraná - Tecpar2005-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400001Brazilian Archives of Biology and Technology v.48 n.spe 2005reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132005000400001info:eu-repo/semantics/openAccessAraújo,José Hilton Bernardino deUemura,Vinicius OliveiraMoraes,Flavio Faria deBarbosa,Aneli de MeloZanin,Gisella Mariaeng2005-08-15T00:00:00Zoai:scielo:S1516-89132005000400001Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2005-08-15T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv A comparative study on fungal laccases immobilized on chitosan
title A comparative study on fungal laccases immobilized on chitosan
spellingShingle A comparative study on fungal laccases immobilized on chitosan
Araújo,José Hilton Bernardino de
Laccases
immobilization
chitosan
title_short A comparative study on fungal laccases immobilized on chitosan
title_full A comparative study on fungal laccases immobilized on chitosan
title_fullStr A comparative study on fungal laccases immobilized on chitosan
title_full_unstemmed A comparative study on fungal laccases immobilized on chitosan
title_sort A comparative study on fungal laccases immobilized on chitosan
author Araújo,José Hilton Bernardino de
author_facet Araújo,José Hilton Bernardino de
Uemura,Vinicius Oliveira
Moraes,Flavio Faria de
Barbosa,Aneli de Melo
Zanin,Gisella Maria
author_role author
author2 Uemura,Vinicius Oliveira
Moraes,Flavio Faria de
Barbosa,Aneli de Melo
Zanin,Gisella Maria
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Araújo,José Hilton Bernardino de
Uemura,Vinicius Oliveira
Moraes,Flavio Faria de
Barbosa,Aneli de Melo
Zanin,Gisella Maria
dc.subject.por.fl_str_mv Laccases
immobilization
chitosan
topic Laccases
immobilization
chitosan
description The phenoloxidase enzyme laccase from the cultures of the Pleurotus ostreatus and Botryosphaeria sp. and a commercial laccase from Aspergillus sp. were immobilized on chitosan of pharmaceutical degree by adsorption followed by crosslinking. Different immobilization conditions in relation to the granulometry of support and amount of enzymatic laccase extract used were tested, aiming at reaching high enzymatic activity with the immobilized enzyme. Two different substrates, ABTS and DMP, were used for the determination of enzymatic activity. The highest enzymatic activity was obtained when 1.0mg/mL of the enzymatic laccase extract from Botryosphaeria sp. was used with 1.0g of support (200 mesh). These immobilized enzymes are to be applied to the improvement of white wines by the degradation of undesirable phenolic compounds.
publishDate 2005
dc.date.none.fl_str_mv 2005-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400001
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400001
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132005000400001
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.48 n.spe 2005
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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