Protective effect of sodium selenite on genotoxicity to human whole blood cultures induced by aflatoxin B1
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000800006 |
Resumo: | The aim of this study was to investigate the effects of selenium and aflatoxin on human whole blood cultures (WBC) in relation to induction of sister-chromatid exchange (SCE). Results showed that the frequency of SCEs in peripheral lymphocytes was significantly increased by the direct-acting mutagen AFB1 (at doses 5 and 10 µM except for 1µM) compared with controls. When sodium selenite (Na2SeO3) was added at a molar ratio of 5x10-7 and 1x10-6, cells did not show significant increase in SCE frequency. Whereas, SCE rates induced by the various AFB1 concentrations could be significantly reduced by the presence of Na2SeO3 in a clear dose-related manner. These results indicated that selenite and AFB1 mutually antagonized their ability to cause DNA damage leading to the formation of SCEs. However, selenium didn't completely inhibit induction of SCEs by AFB1 compared with controls. AFB1 induced oxidative damage contributed to its genotoxicity in human WBC. |
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Brazilian Archives of Biology and Technology |
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Protective effect of sodium selenite on genotoxicity to human whole blood cultures induced by aflatoxin B1Aflatoxin B1SeleniumSister-chromatid exchangesGenotoxicityBloodThe aim of this study was to investigate the effects of selenium and aflatoxin on human whole blood cultures (WBC) in relation to induction of sister-chromatid exchange (SCE). Results showed that the frequency of SCEs in peripheral lymphocytes was significantly increased by the direct-acting mutagen AFB1 (at doses 5 and 10 µM except for 1µM) compared with controls. When sodium selenite (Na2SeO3) was added at a molar ratio of 5x10-7 and 1x10-6, cells did not show significant increase in SCE frequency. Whereas, SCE rates induced by the various AFB1 concentrations could be significantly reduced by the presence of Na2SeO3 in a clear dose-related manner. These results indicated that selenite and AFB1 mutually antagonized their ability to cause DNA damage leading to the formation of SCEs. However, selenium didn't completely inhibit induction of SCEs by AFB1 compared with controls. AFB1 induced oxidative damage contributed to its genotoxicity in human WBC.Instituto de Tecnologia do Paraná - Tecpar2005-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000800006Brazilian Archives of Biology and Technology v.48 n.6 2005reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132005000800006info:eu-repo/semantics/openAccessGeyikoglu,FatimeTürkez,Hasaneng2006-01-20T00:00:00Zoai:scielo:S1516-89132005000800006Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2006-01-20T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Protective effect of sodium selenite on genotoxicity to human whole blood cultures induced by aflatoxin B1 |
title |
Protective effect of sodium selenite on genotoxicity to human whole blood cultures induced by aflatoxin B1 |
spellingShingle |
Protective effect of sodium selenite on genotoxicity to human whole blood cultures induced by aflatoxin B1 Geyikoglu,Fatime Aflatoxin B1 Selenium Sister-chromatid exchanges Genotoxicity Blood |
title_short |
Protective effect of sodium selenite on genotoxicity to human whole blood cultures induced by aflatoxin B1 |
title_full |
Protective effect of sodium selenite on genotoxicity to human whole blood cultures induced by aflatoxin B1 |
title_fullStr |
Protective effect of sodium selenite on genotoxicity to human whole blood cultures induced by aflatoxin B1 |
title_full_unstemmed |
Protective effect of sodium selenite on genotoxicity to human whole blood cultures induced by aflatoxin B1 |
title_sort |
Protective effect of sodium selenite on genotoxicity to human whole blood cultures induced by aflatoxin B1 |
author |
Geyikoglu,Fatime |
author_facet |
Geyikoglu,Fatime Türkez,Hasan |
author_role |
author |
author2 |
Türkez,Hasan |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Geyikoglu,Fatime Türkez,Hasan |
dc.subject.por.fl_str_mv |
Aflatoxin B1 Selenium Sister-chromatid exchanges Genotoxicity Blood |
topic |
Aflatoxin B1 Selenium Sister-chromatid exchanges Genotoxicity Blood |
description |
The aim of this study was to investigate the effects of selenium and aflatoxin on human whole blood cultures (WBC) in relation to induction of sister-chromatid exchange (SCE). Results showed that the frequency of SCEs in peripheral lymphocytes was significantly increased by the direct-acting mutagen AFB1 (at doses 5 and 10 µM except for 1µM) compared with controls. When sodium selenite (Na2SeO3) was added at a molar ratio of 5x10-7 and 1x10-6, cells did not show significant increase in SCE frequency. Whereas, SCE rates induced by the various AFB1 concentrations could be significantly reduced by the presence of Na2SeO3 in a clear dose-related manner. These results indicated that selenite and AFB1 mutually antagonized their ability to cause DNA damage leading to the formation of SCEs. However, selenium didn't completely inhibit induction of SCEs by AFB1 compared with controls. AFB1 induced oxidative damage contributed to its genotoxicity in human WBC. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-11-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000800006 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000800006 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-89132005000800006 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.48 n.6 2005 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318270711332864 |