The construction of a short gene by a very fast, modified, and simplified gene synthesis and the analysis of various effects on this synthesis

Detalhes bibliográficos
Autor(a) principal: Hajjari,Mohammadreza
Data de Publicação: 2011
Outros Autores: Saffar,Behnaz
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100007
Resumo: We have constructed a short gene with a single step assembly PCR without any additional amplification primers with Taq and Pfu polymerases. Since the Taq polymerase is a common and conventional enzyme for PCR reactions, we have analyzed various effects on its efficiency. Eventually, we have been able to synthesize the gene in less than 40 minutes by Taq polymerase.
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spelling The construction of a short gene by a very fast, modified, and simplified gene synthesis and the analysis of various effects on this synthesissingle step assembly PCRefficiencyamplification primerpolymeraseWe have constructed a short gene with a single step assembly PCR without any additional amplification primers with Taq and Pfu polymerases. Since the Taq polymerase is a common and conventional enzyme for PCR reactions, we have analyzed various effects on its efficiency. Eventually, we have been able to synthesize the gene in less than 40 minutes by Taq polymerase.Instituto de Tecnologia do Paraná - Tecpar2011-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100007Brazilian Archives of Biology and Technology v.54 n.1 2011reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132011000100007info:eu-repo/semantics/openAccessHajjari,MohammadrezaSaffar,Behnazeng2011-03-03T00:00:00Zoai:scielo:S1516-89132011000100007Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2011-03-03T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv The construction of a short gene by a very fast, modified, and simplified gene synthesis and the analysis of various effects on this synthesis
title The construction of a short gene by a very fast, modified, and simplified gene synthesis and the analysis of various effects on this synthesis
spellingShingle The construction of a short gene by a very fast, modified, and simplified gene synthesis and the analysis of various effects on this synthesis
Hajjari,Mohammadreza
single step assembly PCR
efficiency
amplification primer
polymerase
title_short The construction of a short gene by a very fast, modified, and simplified gene synthesis and the analysis of various effects on this synthesis
title_full The construction of a short gene by a very fast, modified, and simplified gene synthesis and the analysis of various effects on this synthesis
title_fullStr The construction of a short gene by a very fast, modified, and simplified gene synthesis and the analysis of various effects on this synthesis
title_full_unstemmed The construction of a short gene by a very fast, modified, and simplified gene synthesis and the analysis of various effects on this synthesis
title_sort The construction of a short gene by a very fast, modified, and simplified gene synthesis and the analysis of various effects on this synthesis
author Hajjari,Mohammadreza
author_facet Hajjari,Mohammadreza
Saffar,Behnaz
author_role author
author2 Saffar,Behnaz
author2_role author
dc.contributor.author.fl_str_mv Hajjari,Mohammadreza
Saffar,Behnaz
dc.subject.por.fl_str_mv single step assembly PCR
efficiency
amplification primer
polymerase
topic single step assembly PCR
efficiency
amplification primer
polymerase
description We have constructed a short gene with a single step assembly PCR without any additional amplification primers with Taq and Pfu polymerases. Since the Taq polymerase is a common and conventional enzyme for PCR reactions, we have analyzed various effects on its efficiency. Eventually, we have been able to synthesize the gene in less than 40 minutes by Taq polymerase.
publishDate 2011
dc.date.none.fl_str_mv 2011-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100007
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132011000100007
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.54 n.1 2011
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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