Cloning and Characterization of 5-enopy ruvylshikimate-3-phosphate Synthase from Fragaria vesca

Detalhes bibliográficos
Autor(a) principal: Liu,Feng
Data de Publicação: 2021
Outros Autores: Ruan,Yingying
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132021000100209
Resumo: Abstract To discover and isolate a glyphosate-resistant gene from Fragaria vesca through gene mining. An open reading frame (ORF) of 1563 bp encoding EPSPSwas amplified from Fragaria vesca (FvEPSPS). FvEPSPS (Genebank: XP004306932.1) encodes a polypeptide of 520 amino acids and it has hightly homologous with EPSPS from other plants. qRT-PCR analysis showed that the FvEPSPS was expressed extensively in all tissues including leaves, roots and stems, with higher expression in leaves. Furthermore, transgenic Arabidopsis Thaliana exhibited 10 mM glyphosate to resistance. Therefore, this research offers a new glyphosate-resistant gene for development of transgenic crops.
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spelling Cloning and Characterization of 5-enopy ruvylshikimate-3-phosphate Synthase from Fragaria vescaFragaria vescaFvEPSPSglyphosatetransformationArabidopsis ThalianaAbstract To discover and isolate a glyphosate-resistant gene from Fragaria vesca through gene mining. An open reading frame (ORF) of 1563 bp encoding EPSPSwas amplified from Fragaria vesca (FvEPSPS). FvEPSPS (Genebank: XP004306932.1) encodes a polypeptide of 520 amino acids and it has hightly homologous with EPSPS from other plants. qRT-PCR analysis showed that the FvEPSPS was expressed extensively in all tissues including leaves, roots and stems, with higher expression in leaves. Furthermore, transgenic Arabidopsis Thaliana exhibited 10 mM glyphosate to resistance. Therefore, this research offers a new glyphosate-resistant gene for development of transgenic crops.Instituto de Tecnologia do Paraná - Tecpar2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132021000100209Brazilian Archives of Biology and Technology v.64 2021reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/1678-4324-2021200316info:eu-repo/semantics/openAccessLiu,FengRuan,Yingyingeng2021-06-07T00:00:00Zoai:scielo:S1516-89132021000100209Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2021-06-07T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Cloning and Characterization of 5-enopy ruvylshikimate-3-phosphate Synthase from Fragaria vesca
title Cloning and Characterization of 5-enopy ruvylshikimate-3-phosphate Synthase from Fragaria vesca
spellingShingle Cloning and Characterization of 5-enopy ruvylshikimate-3-phosphate Synthase from Fragaria vesca
Liu,Feng
Fragaria vesca
FvEPSPS
glyphosate
transformation
Arabidopsis Thaliana
title_short Cloning and Characterization of 5-enopy ruvylshikimate-3-phosphate Synthase from Fragaria vesca
title_full Cloning and Characterization of 5-enopy ruvylshikimate-3-phosphate Synthase from Fragaria vesca
title_fullStr Cloning and Characterization of 5-enopy ruvylshikimate-3-phosphate Synthase from Fragaria vesca
title_full_unstemmed Cloning and Characterization of 5-enopy ruvylshikimate-3-phosphate Synthase from Fragaria vesca
title_sort Cloning and Characterization of 5-enopy ruvylshikimate-3-phosphate Synthase from Fragaria vesca
author Liu,Feng
author_facet Liu,Feng
Ruan,Yingying
author_role author
author2 Ruan,Yingying
author2_role author
dc.contributor.author.fl_str_mv Liu,Feng
Ruan,Yingying
dc.subject.por.fl_str_mv Fragaria vesca
FvEPSPS
glyphosate
transformation
Arabidopsis Thaliana
topic Fragaria vesca
FvEPSPS
glyphosate
transformation
Arabidopsis Thaliana
description Abstract To discover and isolate a glyphosate-resistant gene from Fragaria vesca through gene mining. An open reading frame (ORF) of 1563 bp encoding EPSPSwas amplified from Fragaria vesca (FvEPSPS). FvEPSPS (Genebank: XP004306932.1) encodes a polypeptide of 520 amino acids and it has hightly homologous with EPSPS from other plants. qRT-PCR analysis showed that the FvEPSPS was expressed extensively in all tissues including leaves, roots and stems, with higher expression in leaves. Furthermore, transgenic Arabidopsis Thaliana exhibited 10 mM glyphosate to resistance. Therefore, this research offers a new glyphosate-resistant gene for development of transgenic crops.
publishDate 2021
dc.date.none.fl_str_mv 2021-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132021000100209
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132021000100209
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1678-4324-2021200316
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.64 2021
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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