Ex Vivo Effect of Ibogaine on the Transcriptional Level of Antioxidant Defense Related Genes in Honey Bee (Apis mellifera, L.) Midgut
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132021000100417 |
Resumo: | Abstract The aim of the present study was to analyze the mechanisms of ibogaine action by measuring its ex vivo effects on antioxidant defense in the honey bee (Apis mellifera, L.) midgut. The transcriptional levels of selected genes: Cu/Zn dependent and Mn dependent superoxide dismutases (Sod1 and Sod2, respectively), catalase (Cat) and transcription factor Nrf2 (Nrf2) were determined. The applicability of midgut tissue, which expected to have well developed antioxidant protection system, for this type of analysis was confirmed by testing cell viability and response to paraquat, an effective inducer of oxidative stress, ex vivo. Incubation for 2 h with paraquat (10 µg/mL) induced a significant increase in expression of Sod1 and Cat genes. The results of ibogaine treatment showed that exposure to 5 µg/mL and 10 µg/mL of ibogaine for 2 h induced significant increase in expression of Sod1 gene. On the other hand, ibogaine did not lead to a significant increase of Sod2, Cat and transcription factor Nrf2 genes expression in honey bee midgut ex vivo. Our results confirmed positive effect of ibogaine on the antioxidant protective system and its pro-antioxidant action. |
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Ex Vivo Effect of Ibogaine on the Transcriptional Level of Antioxidant Defense Related Genes in Honey Bee (Apis mellifera, L.) MidgutIbogaineParaquatInsect midgutSuperoxide dismutaseCatalaseAbstract The aim of the present study was to analyze the mechanisms of ibogaine action by measuring its ex vivo effects on antioxidant defense in the honey bee (Apis mellifera, L.) midgut. The transcriptional levels of selected genes: Cu/Zn dependent and Mn dependent superoxide dismutases (Sod1 and Sod2, respectively), catalase (Cat) and transcription factor Nrf2 (Nrf2) were determined. The applicability of midgut tissue, which expected to have well developed antioxidant protection system, for this type of analysis was confirmed by testing cell viability and response to paraquat, an effective inducer of oxidative stress, ex vivo. Incubation for 2 h with paraquat (10 µg/mL) induced a significant increase in expression of Sod1 and Cat genes. The results of ibogaine treatment showed that exposure to 5 µg/mL and 10 µg/mL of ibogaine for 2 h induced significant increase in expression of Sod1 gene. On the other hand, ibogaine did not lead to a significant increase of Sod2, Cat and transcription factor Nrf2 genes expression in honey bee midgut ex vivo. Our results confirmed positive effect of ibogaine on the antioxidant protective system and its pro-antioxidant action.Instituto de Tecnologia do Paraná - Tecpar2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132021000100417Brazilian Archives of Biology and Technology v.64 2021reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/1678-4324-2021200773info:eu-repo/semantics/openAccessVukašinović,ElviraPurać,JelenaKojić,DanijelaČelić,TatjanaPihler,IvanBlagojević,Duškoeng2021-11-17T00:00:00Zoai:scielo:S1516-89132021000100417Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2021-11-17T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Ex Vivo Effect of Ibogaine on the Transcriptional Level of Antioxidant Defense Related Genes in Honey Bee (Apis mellifera, L.) Midgut |
title |
Ex Vivo Effect of Ibogaine on the Transcriptional Level of Antioxidant Defense Related Genes in Honey Bee (Apis mellifera, L.) Midgut |
spellingShingle |
Ex Vivo Effect of Ibogaine on the Transcriptional Level of Antioxidant Defense Related Genes in Honey Bee (Apis mellifera, L.) Midgut Vukašinović,Elvira Ibogaine Paraquat Insect midgut Superoxide dismutase Catalase |
title_short |
Ex Vivo Effect of Ibogaine on the Transcriptional Level of Antioxidant Defense Related Genes in Honey Bee (Apis mellifera, L.) Midgut |
title_full |
Ex Vivo Effect of Ibogaine on the Transcriptional Level of Antioxidant Defense Related Genes in Honey Bee (Apis mellifera, L.) Midgut |
title_fullStr |
Ex Vivo Effect of Ibogaine on the Transcriptional Level of Antioxidant Defense Related Genes in Honey Bee (Apis mellifera, L.) Midgut |
title_full_unstemmed |
Ex Vivo Effect of Ibogaine on the Transcriptional Level of Antioxidant Defense Related Genes in Honey Bee (Apis mellifera, L.) Midgut |
title_sort |
Ex Vivo Effect of Ibogaine on the Transcriptional Level of Antioxidant Defense Related Genes in Honey Bee (Apis mellifera, L.) Midgut |
author |
Vukašinović,Elvira |
author_facet |
Vukašinović,Elvira Purać,Jelena Kojić,Danijela Čelić,Tatjana Pihler,Ivan Blagojević,Duško |
author_role |
author |
author2 |
Purać,Jelena Kojić,Danijela Čelić,Tatjana Pihler,Ivan Blagojević,Duško |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Vukašinović,Elvira Purać,Jelena Kojić,Danijela Čelić,Tatjana Pihler,Ivan Blagojević,Duško |
dc.subject.por.fl_str_mv |
Ibogaine Paraquat Insect midgut Superoxide dismutase Catalase |
topic |
Ibogaine Paraquat Insect midgut Superoxide dismutase Catalase |
description |
Abstract The aim of the present study was to analyze the mechanisms of ibogaine action by measuring its ex vivo effects on antioxidant defense in the honey bee (Apis mellifera, L.) midgut. The transcriptional levels of selected genes: Cu/Zn dependent and Mn dependent superoxide dismutases (Sod1 and Sod2, respectively), catalase (Cat) and transcription factor Nrf2 (Nrf2) were determined. The applicability of midgut tissue, which expected to have well developed antioxidant protection system, for this type of analysis was confirmed by testing cell viability and response to paraquat, an effective inducer of oxidative stress, ex vivo. Incubation for 2 h with paraquat (10 µg/mL) induced a significant increase in expression of Sod1 and Cat genes. The results of ibogaine treatment showed that exposure to 5 µg/mL and 10 µg/mL of ibogaine for 2 h induced significant increase in expression of Sod1 gene. On the other hand, ibogaine did not lead to a significant increase of Sod2, Cat and transcription factor Nrf2 genes expression in honey bee midgut ex vivo. Our results confirmed positive effect of ibogaine on the antioxidant protective system and its pro-antioxidant action. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132021000100417 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132021000100417 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-4324-2021200773 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.64 2021 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318280558510080 |