Expression of a methionine-rich storage albumin from the Brazil nut (Bertholletia excelsa H.B.K., Lecythidaceae) in transgenic bean plants (Phaseolus vulgaris L., Fabaceae)

• Autor(a) principal: Aragão, Francisco José Lima
• Data de Publicação: 1999
• Outros Autores: Barros, Leila Maria Gomes, Grossi-de-Sá, Maria Fatima, Almeida, Elionor Rita Pereira de, Gander, Eugen Silvano, Rech Filho, Elibio Leopoldo
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UCB
• Texto Completo: http://twingo.ucb.br:8080/jspui/handle/10869/515; https://repositorio.ucb.br:9443/jspui/handle/123456789/7694

Bean (Phaseolus vulgaris), an important component in the diet of people in developing countries, has low levels of the essential amino acid, methionine. We have attempted to correct this deficiency by introducing a transgene coding for a methionine-rich storage albumin from the Brazil nut via biolistic methods. The transgene's coding sequence was driven by a doubled 35S CaMV promoter and AMV enhancer sequences. The transgene was stable and correctly expressed in homozygous R2 to R5 seeds. In two of the five transgenic lines the methionine content was significantly increased (14 and 23%) over the values found in untransformed plants.