Expression of a methionine-rich storage albumin from the Brazil nut (Bertholletia excelsa H.B.K., Lecythidaceae) in transgenic bean plants (Phaseolus vulgaris L., Fabaceae)

Detalhes bibliográficos
Autor(a) principal: Aragão, F.J.L.
Data de Publicação: 1999
Outros Autores: Barros, L.M.G., Sousa, M.V. de, Grossi de Sá, M.F., Almeida, E.R.P., Gander, E.S., Rech, E.L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UnB
Texto Completo: http://repositorio.unb.br/handle/10482/25558
https://dx.doi.org/10.1590/S1415-47571999000300026
Resumo: Bean (Phaseolus vulgaris), an important component in the diet of people in developing countries, has low levels of the essential amino acid, methionine. We have attempted to correct this deficiency by introducing a transgene coding for a methionine-rich storage albumin from the Brazil nut via biolistic methods. The transgene's coding sequence was driven by a doubled 35S CaMV promoter and AMV enhancer sequences. The transgene was stable and correctly expressed in homozygous R2 to R5 seeds. In two of the five transgenic lines the methionine content was significantly increased (14 and 23%) over the values found in untransformed plants.
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spelling Expression of a methionine-rich storage albumin from the Brazil nut (Bertholletia excelsa H.B.K., Lecythidaceae) in transgenic bean plants (Phaseolus vulgaris L., Fabaceae)Bean (Phaseolus vulgaris), an important component in the diet of people in developing countries, has low levels of the essential amino acid, methionine. We have attempted to correct this deficiency by introducing a transgene coding for a methionine-rich storage albumin from the Brazil nut via biolistic methods. The transgene's coding sequence was driven by a doubled 35S CaMV promoter and AMV enhancer sequences. The transgene was stable and correctly expressed in homozygous R2 to R5 seeds. In two of the five transgenic lines the methionine content was significantly increased (14 and 23%) over the values found in untransformed plants.O feijão (Phaseolus vulgaris L.) é um componente importante na dieta da população de países em desenvolvimento. Entretanto, possui um baixo nível de aminoácidos essenciais, como a metionina. Numa tentativa de corrigir esta deficiência, plantas transgênicas de feijão foram produzidas contendo o gene de uma proteína rica em metionina, a albumina 2S da castanha do Brasil. O gene desta albumina (be2s2), clonado sob o controle do promotor 35S dobrado do vírus do mosaico da couve-flor e uma seqüência "enhancer" do vírus do mosaico da alfafa, foi introduzido em feijão através do processo biobalístico. O gene foi expressado corretamente em sementes homozigotas desde a segunda até a quinta geração. Em duas linhagens transgênicas o nível de metionina foi incrementado em 14 e 23% nas sementes.Em processamentoSociedade Brasileira de Genética2017-12-07T04:31:52Z2017-12-07T04:31:52Z1999info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfGenet. Mol. Biol.,v.22,n.3,p.445-449,1999http://repositorio.unb.br/handle/10482/25558https://dx.doi.org/10.1590/S1415-47571999000300026Aragão, F.J.L.Barros, L.M.G.Sousa, M.V. deGrossi de Sá, M.F.Almeida, E.R.P.Gander, E.S.Rech, E.L.info:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNB2023-10-06T19:12:22Zoai:repositorio.unb.br:10482/25558Repositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2023-10-06T19:12:22Repositório Institucional da UnB - Universidade de Brasília (UnB)false
dc.title.none.fl_str_mv Expression of a methionine-rich storage albumin from the Brazil nut (Bertholletia excelsa H.B.K., Lecythidaceae) in transgenic bean plants (Phaseolus vulgaris L., Fabaceae)
title Expression of a methionine-rich storage albumin from the Brazil nut (Bertholletia excelsa H.B.K., Lecythidaceae) in transgenic bean plants (Phaseolus vulgaris L., Fabaceae)
spellingShingle Expression of a methionine-rich storage albumin from the Brazil nut (Bertholletia excelsa H.B.K., Lecythidaceae) in transgenic bean plants (Phaseolus vulgaris L., Fabaceae)
Aragão, F.J.L.
title_short Expression of a methionine-rich storage albumin from the Brazil nut (Bertholletia excelsa H.B.K., Lecythidaceae) in transgenic bean plants (Phaseolus vulgaris L., Fabaceae)
title_full Expression of a methionine-rich storage albumin from the Brazil nut (Bertholletia excelsa H.B.K., Lecythidaceae) in transgenic bean plants (Phaseolus vulgaris L., Fabaceae)
title_fullStr Expression of a methionine-rich storage albumin from the Brazil nut (Bertholletia excelsa H.B.K., Lecythidaceae) in transgenic bean plants (Phaseolus vulgaris L., Fabaceae)
title_full_unstemmed Expression of a methionine-rich storage albumin from the Brazil nut (Bertholletia excelsa H.B.K., Lecythidaceae) in transgenic bean plants (Phaseolus vulgaris L., Fabaceae)
title_sort Expression of a methionine-rich storage albumin from the Brazil nut (Bertholletia excelsa H.B.K., Lecythidaceae) in transgenic bean plants (Phaseolus vulgaris L., Fabaceae)
author Aragão, F.J.L.
author_facet Aragão, F.J.L.
Barros, L.M.G.
Sousa, M.V. de
Grossi de Sá, M.F.
Almeida, E.R.P.
Gander, E.S.
Rech, E.L.
author_role author
author2 Barros, L.M.G.
Sousa, M.V. de
Grossi de Sá, M.F.
Almeida, E.R.P.
Gander, E.S.
Rech, E.L.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Aragão, F.J.L.
Barros, L.M.G.
Sousa, M.V. de
Grossi de Sá, M.F.
Almeida, E.R.P.
Gander, E.S.
Rech, E.L.
description Bean (Phaseolus vulgaris), an important component in the diet of people in developing countries, has low levels of the essential amino acid, methionine. We have attempted to correct this deficiency by introducing a transgene coding for a methionine-rich storage albumin from the Brazil nut via biolistic methods. The transgene's coding sequence was driven by a doubled 35S CaMV promoter and AMV enhancer sequences. The transgene was stable and correctly expressed in homozygous R2 to R5 seeds. In two of the five transgenic lines the methionine content was significantly increased (14 and 23%) over the values found in untransformed plants.
publishDate 1999
dc.date.none.fl_str_mv 1999
2017-12-07T04:31:52Z
2017-12-07T04:31:52Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Genet. Mol. Biol.,v.22,n.3,p.445-449,1999
http://repositorio.unb.br/handle/10482/25558
https://dx.doi.org/10.1590/S1415-47571999000300026
identifier_str_mv Genet. Mol. Biol.,v.22,n.3,p.445-449,1999
url http://repositorio.unb.br/handle/10482/25558
https://dx.doi.org/10.1590/S1415-47571999000300026
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dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv reponame:Repositório Institucional da UnB
instname:Universidade de Brasília (UnB)
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reponame_str Repositório Institucional da UnB
collection Repositório Institucional da UnB
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