Mapping of the conserved antigenic domains shared between potato apyrase and parasite ATP diphosphohydrolases: potential application in human parasitic diseases

• Autor(a) principal: Faria-Pinto, Priscila de
• Data de Publicação: 2008
• Outros Autores: Soares, Fabrícia Aparecida Rezende, Molica, Andréia Maria, Montesano, Maria Angela, Marques, Marcos José, Rocha, Manoel Otávio da Costa, Coelho, Paulo Marcos Zech, Gomes, Juliana de Assis Silva, Enk, Martin Johannes, Oliveira, Rodrigo Corrêa, Maria Neto, Simone, Franco, Octávio Luiz, Vasconcelos, Eveline Gomes
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UCB
• Texto Completo: http://twingo.ucb.br:8080/jspui/handle/10869/682; https://repositorio.ucb.br:9443/jspui/handle/123456789/7841
• topic: ATP diphosphohydrolase; Apyrase; NTPDase; Leishmania braziliensis; Schistosoma mansoni; Trypanosoma cruzi; Solanum tuberosum; Antibody; Molecular modelling; Phylogenetic

Evolutionary and closer structural relationships are demonstrated by phylogenetic analysis, peptide prediction and molecular modelling between Solanum tuberosum apyrase, Schistosoma mansoni SmATPase 2 and Leishmania braziliensis NDPase. Specific protein domains are suggested to be potentially involved in the immune response, and also seem to be conserved during host and parasite co-evolution. Significant IgG antibody reactivity was observed in sera from patients with American cutaneous leishmaniasis (ACL) and schistosomiasis using potato apyrase as antigen in ELISA. S. mansoni adult worm or egg, L. braziliensis promastigote (Lb) and Trypanosoma cruzi epimastigote (EPI) have ATP diphosphohydrolases, and antigenic preparations of them were evaluated. In ACL patients, IgG seropositivity was about 43% and 90% for Lb and potato apyrase, respectively, while IgM was lower (<19%) for both. In schistosomiasis patients IgM (>40%) or IgG (100%) seropositivity for both soluble egg (SEA) and adult worm (SWAP) antigens was higher than that found for potato apyrase (IgM=10%; IgG=39%). In Chagas disease, IgG seropositivity for EPI and potato apyrase was 97% and 17%, respectively, while the IgM was low (3%) for both antigens. The study of the conserved domains from both parasite proteins and potato apyrase could lead to the development of new drug targets or molecular markers.