Production of Polypeptin A analogues by Paenibacillus elgii strain AC13 and quorum sensing dependent bacterial processes

Detalhes bibliográficos
Autor(a) principal: Ortega, Daniel Barros
Data de Publicação: 2019
Tipo de documento: Tese
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UCB
Texto Completo: https://bdtd.ucb.br:8443/jspui/handle/tede/2603
Resumo: Quorum sensing (QS) are sophisticated communication networks based on metabolic exchanges that influence bacteria processes such as reproduction, sporulation, biofilm formation, virulence and antimicrobials expression. Non-ribosomal peptides (NRP) use non-proteinogenic amino acids as building blocks, which increase the number of possible templates when compared to standard ribosomal synthesis process. Naturally produced by Paenibacillus elgii, the NRP family of Pelgipeptins (PGP) consists of five cyclic and cationic lipopeptides comprising nine amino acids and a fatty acid chain. Linearization of NRP similar to PGPs have improved antifungal activity against pathogenic Candida albicans. Paenibacillus elgii strain AC13 was isolated from soil samples of the Brazilian Cerrado. PGPs produced by AC13 were active against Escherichia coli, Staphylococcus aureus and multidrug resistant Klebsiella pneumoniae. Strain AC13 also synthesizes linear counterparts or PGPs in nutrient broth. Growth parameters, PGPs quantification, and partial characterization of produced lipopeptides suggest that strain AC13 is able to modulate its lipopeptides production based on variations of its environment or initial population. Molecular networking analysis indicates that the precursor mass of 1,101.7052 Da (PGP-B) is connected to the gene leuB pathway, responsible to code 3-isopropylmalate dehydrogenase described for P. dendritiformis C454, P. selenitireducens, P. yonginensis and P. amylolyticus. The enzyme is part of leucine biosynthetic pathway and is responsible to catalyze the oxidation of 3-isopropylmalate. Molecular networking analysis also indicates that the enzyme responsible to synthesize O-acetyl-L-homoserine (gene metAA) expressed by Lysinibacillus sp. strain FJAT-14222 is connected to precursor mass 416.7368 Da. Among QS signaling pathways, N-acyl-homoserine lactones occur as signaling molecules for more than 70 different species of diderm bacteria. QS communication can be interrupted by reducing the activity of signaling receptor proteins, their synthesis or by synthetic molecules analogous to QS signaling compounds, increasing the strategies on fighting against multidrug resistant microorganisms. Notwithstanding, a better understanding of the overlap between system biology and advanced molecular network techniques is needed.
id UCB_37525f748fd432e5d4405eca81c7b027
oai_identifier_str oai:bdtd.ucb.br:tede/2603
network_acronym_str UCB
network_name_str Biblioteca Digital de Teses e Dissertações da UCB
spelling Barreto, Cristine Chaveshttp://lattes.cnpq.br/9973148799581550http://lattes.cnpq.br/2389648101878531Ortega, Daniel Barros2019-06-17T19:47:29Z2019-03-25ORTEGA, Daniel Barros. Production of Polypeptin A analogues by Paenibacillus elgii strain AC13 and quorum sensing dependent bacterial processes. 2019. 126 f. Tese (Programa Stricto Sensu em Ci??ncias Gen??micas e Biotecnologia) - Universidade Cat??lica de Bras??lia, Bras??lia, 2019.https://bdtd.ucb.br:8443/jspui/handle/tede/2603Quorum sensing (QS) are sophisticated communication networks based on metabolic exchanges that influence bacteria processes such as reproduction, sporulation, biofilm formation, virulence and antimicrobials expression. Non-ribosomal peptides (NRP) use non-proteinogenic amino acids as building blocks, which increase the number of possible templates when compared to standard ribosomal synthesis process. Naturally produced by Paenibacillus elgii, the NRP family of Pelgipeptins (PGP) consists of five cyclic and cationic lipopeptides comprising nine amino acids and a fatty acid chain. Linearization of NRP similar to PGPs have improved antifungal activity against pathogenic Candida albicans. Paenibacillus elgii strain AC13 was isolated from soil samples of the Brazilian Cerrado. PGPs produced by AC13 were active against Escherichia coli, Staphylococcus aureus and multidrug resistant Klebsiella pneumoniae. Strain AC13 also synthesizes linear counterparts or PGPs in nutrient broth. Growth parameters, PGPs quantification, and partial characterization of produced lipopeptides suggest that strain AC13 is able to modulate its lipopeptides production based on variations of its environment or initial population. Molecular networking analysis indicates that the precursor mass of 1,101.7052 Da (PGP-B) is connected to the gene leuB pathway, responsible to code 3-isopropylmalate dehydrogenase described for P. dendritiformis C454, P. selenitireducens, P. yonginensis and P. amylolyticus. The enzyme is part of leucine biosynthetic pathway and is responsible to catalyze the oxidation of 3-isopropylmalate. Molecular networking analysis also indicates that the enzyme responsible to synthesize O-acetyl-L-homoserine (gene metAA) expressed by Lysinibacillus sp. strain FJAT-14222 is connected to precursor mass 416.7368 Da. Among QS signaling pathways, N-acyl-homoserine lactones occur as signaling molecules for more than 70 different species of diderm bacteria. QS communication can be interrupted by reducing the activity of signaling receptor proteins, their synthesis or by synthetic molecules analogous to QS signaling compounds, increasing the strategies on fighting against multidrug resistant microorganisms. Notwithstanding, a better understanding of the overlap between system biology and advanced molecular network techniques is needed.Quorum sensing (QS) s??o sofisticadas redes de comunica????o baseadas em trocas metab??licas que influenciam processos bacterianos como a bioluminesc??ncia, reprodu????o, esporula????o, forma????o de biofilme, express??o de virul??ncia e produ????o de antimicrobianos. Pept??deos n??o ribossomais (NRP) utilizam amino??cidos n??o proteinog??nicos como blocos de constru????o, aumentando o n??mero de poss??veis arcabou??os quando comparados aos obtidos pelo processo de s??ntese riboss??mica. Naturalmente produzida por Paenibacillus elgii, a fam??lia das Pelgipeptinas (PGP) ?? constitu??da por cinco NRP c??clicos e cati??nicos compostos por nove amino??cidos e uma cadeia de ??cido graxo. Ap??s a lineariza????o de NRP semelhantes ??s PGP, concentra????es mais baixas foram suficientes para inibi????o do crescimento de Candida albicans patog??nica, ampliando a polival??ncia deste grupo de mol??culas. A cepa de P. elgii AC13 foi isolada de amostras de solo do Cerrado brasileiro. PGP produzidas por AC13 tiveram atividade contra Escherichia coli, Staphylococcus aureus e Klebsiella pneumoniae multirresistente. Isoformas lineares de PGP tamb??m foram encontradas em culturas de AC13 em caldo nutriente. Par??metros de crescimento, quantifica????o de PGP e caracteriza????o parcial de lipopept??deos produzidos sugerem que a cepa AC13 ?? capaz de modular a produ????o PGP em fun????o de varia????es de seu ambiente ou de sua popula????o inicial. Associa????es por Redes Moleculares indicam que o ??on de massa 1.101,7052 Da (PGP-B) relaciona-se ao gene leu B, respons??vel por codificar 3-isopropilmalato desidrogenase em P. dendritiformis C454, P. selenitireducens, P. yonginensis e P. amylolyticus. A enzima ?? parte da via biossint??tica da leucina e ?? respons??vel por catalisar a oxida????o do 3-isopropilmalato. Analises semelhantes tamb??m sugerem que a enzima respons??vel por sintetizar O-acetil-L-homoserina em Lysinibacillus sp. cepa FJAT-14222 est?? relacionada ao ??on 416.7368 Da, ambos encontrados em cultura de AC13 por LC-MS. Entre as vias de sinaliza????o de QS, N-acil-homoserina lactonas s??o mol??culas de sinaliza????o para mais de 70 esp??cies diferentes de bact??rias Gram-negativas. Como forma de se aumentar as estrat??gias no combate aos microrganismos multirresistentes, sugere-se a interfer??ncia nos sistemas de comunica????o QS, que podem ser interrompidos pela redu????o da atividade ou bloqueio da s??ntese de prote??nas receptoras de sinaliza????o ou ainda por meio de mol??culas sint??ticas an??logas aos compostos sinalizadores de QS. Contudo, uma melhor compreens??o da interface entre a biologia de sistemas e t??cnicas avan??adas de redes moleculares se faz necess??ria.Submitted by Sara Ribeiro (sara.ribeiro@ucb.br) on 2019-06-17T19:47:19Z No. of bitstreams: 1 DanielBarrosOrtegaTese2019.pdf: 3443608 bytes, checksum: c59ff4cc6eb4e452396e9ee3d1371beb (MD5)Approved for entry into archive by Sara Ribeiro (sara.ribeiro@ucb.br) on 2019-06-17T19:47:29Z (GMT) No. of bitstreams: 1 DanielBarrosOrtegaTese2019.pdf: 3443608 bytes, checksum: c59ff4cc6eb4e452396e9ee3d1371beb (MD5)Made available in DSpace on 2019-06-17T19:47:29Z (GMT). No. of bitstreams: 1 DanielBarrosOrtegaTese2019.pdf: 3443608 bytes, checksum: c59ff4cc6eb4e452396e9ee3d1371beb (MD5) Previous issue date: 2019-03-25application/pdfhttps://bdtd.ucb.br:8443/jspui/retrieve/6514/DanielBarrosOrtegaTese2019.pdf.jpgporUniversidade Cat??lica de Bras??liaPrograma Stricto Sensu em Ci??ncias Gen??micas e BiotecnologiaUCBBrasilEscola de Sa??de e MedicinaPaenibacillusPelgipeptinasQuorum-sensingMicrobiologia de solosSoil microbiologyCNPQ::CIENCIAS BIOLOGICASProduction of Polypeptin A analogues by Paenibacillus elgii strain AC13 and quorum sensing dependent bacterial processesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UCBinstname:Universidade Católica de Brasília (UCB)instacron:UCBLICENSElicense.txtlicense.txttext/plain; charset=utf-81831https://200.214.135.178:8443/jspui/bitstream/tede/2603/1/license.txtd7d5e5ec75089f122abe937645a56120MD51ORIGINALDanielBarrosOrtegaTese2019.pdfDanielBarrosOrtegaTese2019.pdfapplication/pdf3443608https://200.214.135.178:8443/jspui/bitstream/tede/2603/2/DanielBarrosOrtegaTese2019.pdfc59ff4cc6eb4e452396e9ee3d1371bebMD52TEXTDanielBarrosOrtegaTese2019.pdf.txtDanielBarrosOrtegaTese2019.pdf.txttext/plain210469https://200.214.135.178:8443/jspui/bitstream/tede/2603/3/DanielBarrosOrtegaTese2019.pdf.txt70e9c6b79911d93ac941e79939d48b68MD53THUMBNAILDanielBarrosOrtegaTese2019.pdf.jpgDanielBarrosOrtegaTese2019.pdf.jpgimage/jpeg5410https://200.214.135.178:8443/jspui/bitstream/tede/2603/4/DanielBarrosOrtegaTese2019.pdf.jpgd9a2e6d73d09d21626810226de388f37MD54tede/26032019-06-18 01:06:09.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Biblioteca Digital de Teses e Dissertaçõeshttps://bdtd.ucb.br:8443/jspui/
dc.title.por.fl_str_mv Production of Polypeptin A analogues by Paenibacillus elgii strain AC13 and quorum sensing dependent bacterial processes
title Production of Polypeptin A analogues by Paenibacillus elgii strain AC13 and quorum sensing dependent bacterial processes
spellingShingle Production of Polypeptin A analogues by Paenibacillus elgii strain AC13 and quorum sensing dependent bacterial processes
Ortega, Daniel Barros
Paenibacillus
Pelgipeptinas
Quorum-sensing
Microbiologia de solos
Soil microbiology
CNPQ::CIENCIAS BIOLOGICAS
title_short Production of Polypeptin A analogues by Paenibacillus elgii strain AC13 and quorum sensing dependent bacterial processes
title_full Production of Polypeptin A analogues by Paenibacillus elgii strain AC13 and quorum sensing dependent bacterial processes
title_fullStr Production of Polypeptin A analogues by Paenibacillus elgii strain AC13 and quorum sensing dependent bacterial processes
title_full_unstemmed Production of Polypeptin A analogues by Paenibacillus elgii strain AC13 and quorum sensing dependent bacterial processes
title_sort Production of Polypeptin A analogues by Paenibacillus elgii strain AC13 and quorum sensing dependent bacterial processes
author Ortega, Daniel Barros
author_facet Ortega, Daniel Barros
author_role author
dc.contributor.advisor1.fl_str_mv Barreto, Cristine Chaves
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/9973148799581550
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/2389648101878531
dc.contributor.author.fl_str_mv Ortega, Daniel Barros
contributor_str_mv Barreto, Cristine Chaves
dc.subject.por.fl_str_mv Paenibacillus
Pelgipeptinas
Quorum-sensing
Microbiologia de solos
topic Paenibacillus
Pelgipeptinas
Quorum-sensing
Microbiologia de solos
Soil microbiology
CNPQ::CIENCIAS BIOLOGICAS
dc.subject.eng.fl_str_mv Soil microbiology
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS BIOLOGICAS
dc.description.abstract.eng.fl_txt_mv Quorum sensing (QS) are sophisticated communication networks based on metabolic exchanges that influence bacteria processes such as reproduction, sporulation, biofilm formation, virulence and antimicrobials expression. Non-ribosomal peptides (NRP) use non-proteinogenic amino acids as building blocks, which increase the number of possible templates when compared to standard ribosomal synthesis process. Naturally produced by Paenibacillus elgii, the NRP family of Pelgipeptins (PGP) consists of five cyclic and cationic lipopeptides comprising nine amino acids and a fatty acid chain. Linearization of NRP similar to PGPs have improved antifungal activity against pathogenic Candida albicans. Paenibacillus elgii strain AC13 was isolated from soil samples of the Brazilian Cerrado. PGPs produced by AC13 were active against Escherichia coli, Staphylococcus aureus and multidrug resistant Klebsiella pneumoniae. Strain AC13 also synthesizes linear counterparts or PGPs in nutrient broth. Growth parameters, PGPs quantification, and partial characterization of produced lipopeptides suggest that strain AC13 is able to modulate its lipopeptides production based on variations of its environment or initial population. Molecular networking analysis indicates that the precursor mass of 1,101.7052 Da (PGP-B) is connected to the gene leuB pathway, responsible to code 3-isopropylmalate dehydrogenase described for P. dendritiformis C454, P. selenitireducens, P. yonginensis and P. amylolyticus. The enzyme is part of leucine biosynthetic pathway and is responsible to catalyze the oxidation of 3-isopropylmalate. Molecular networking analysis also indicates that the enzyme responsible to synthesize O-acetyl-L-homoserine (gene metAA) expressed by Lysinibacillus sp. strain FJAT-14222 is connected to precursor mass 416.7368 Da. Among QS signaling pathways, N-acyl-homoserine lactones occur as signaling molecules for more than 70 different species of diderm bacteria. QS communication can be interrupted by reducing the activity of signaling receptor proteins, their synthesis or by synthetic molecules analogous to QS signaling compounds, increasing the strategies on fighting against multidrug resistant microorganisms. Notwithstanding, a better understanding of the overlap between system biology and advanced molecular network techniques is needed.
dc.description.abstract.por.fl_txt_mv Quorum sensing (QS) s??o sofisticadas redes de comunica????o baseadas em trocas metab??licas que influenciam processos bacterianos como a bioluminesc??ncia, reprodu????o, esporula????o, forma????o de biofilme, express??o de virul??ncia e produ????o de antimicrobianos. Pept??deos n??o ribossomais (NRP) utilizam amino??cidos n??o proteinog??nicos como blocos de constru????o, aumentando o n??mero de poss??veis arcabou??os quando comparados aos obtidos pelo processo de s??ntese riboss??mica. Naturalmente produzida por Paenibacillus elgii, a fam??lia das Pelgipeptinas (PGP) ?? constitu??da por cinco NRP c??clicos e cati??nicos compostos por nove amino??cidos e uma cadeia de ??cido graxo. Ap??s a lineariza????o de NRP semelhantes ??s PGP, concentra????es mais baixas foram suficientes para inibi????o do crescimento de Candida albicans patog??nica, ampliando a polival??ncia deste grupo de mol??culas. A cepa de P. elgii AC13 foi isolada de amostras de solo do Cerrado brasileiro. PGP produzidas por AC13 tiveram atividade contra Escherichia coli, Staphylococcus aureus e Klebsiella pneumoniae multirresistente. Isoformas lineares de PGP tamb??m foram encontradas em culturas de AC13 em caldo nutriente. Par??metros de crescimento, quantifica????o de PGP e caracteriza????o parcial de lipopept??deos produzidos sugerem que a cepa AC13 ?? capaz de modular a produ????o PGP em fun????o de varia????es de seu ambiente ou de sua popula????o inicial. Associa????es por Redes Moleculares indicam que o ??on de massa 1.101,7052 Da (PGP-B) relaciona-se ao gene leu B, respons??vel por codificar 3-isopropilmalato desidrogenase em P. dendritiformis C454, P. selenitireducens, P. yonginensis e P. amylolyticus. A enzima ?? parte da via biossint??tica da leucina e ?? respons??vel por catalisar a oxida????o do 3-isopropilmalato. Analises semelhantes tamb??m sugerem que a enzima respons??vel por sintetizar O-acetil-L-homoserina em Lysinibacillus sp. cepa FJAT-14222 est?? relacionada ao ??on 416.7368 Da, ambos encontrados em cultura de AC13 por LC-MS. Entre as vias de sinaliza????o de QS, N-acil-homoserina lactonas s??o mol??culas de sinaliza????o para mais de 70 esp??cies diferentes de bact??rias Gram-negativas. Como forma de se aumentar as estrat??gias no combate aos microrganismos multirresistentes, sugere-se a interfer??ncia nos sistemas de comunica????o QS, que podem ser interrompidos pela redu????o da atividade ou bloqueio da s??ntese de prote??nas receptoras de sinaliza????o ou ainda por meio de mol??culas sint??ticas an??logas aos compostos sinalizadores de QS. Contudo, uma melhor compreens??o da interface entre a biologia de sistemas e t??cnicas avan??adas de redes moleculares se faz necess??ria.
description Quorum sensing (QS) are sophisticated communication networks based on metabolic exchanges that influence bacteria processes such as reproduction, sporulation, biofilm formation, virulence and antimicrobials expression. Non-ribosomal peptides (NRP) use non-proteinogenic amino acids as building blocks, which increase the number of possible templates when compared to standard ribosomal synthesis process. Naturally produced by Paenibacillus elgii, the NRP family of Pelgipeptins (PGP) consists of five cyclic and cationic lipopeptides comprising nine amino acids and a fatty acid chain. Linearization of NRP similar to PGPs have improved antifungal activity against pathogenic Candida albicans. Paenibacillus elgii strain AC13 was isolated from soil samples of the Brazilian Cerrado. PGPs produced by AC13 were active against Escherichia coli, Staphylococcus aureus and multidrug resistant Klebsiella pneumoniae. Strain AC13 also synthesizes linear counterparts or PGPs in nutrient broth. Growth parameters, PGPs quantification, and partial characterization of produced lipopeptides suggest that strain AC13 is able to modulate its lipopeptides production based on variations of its environment or initial population. Molecular networking analysis indicates that the precursor mass of 1,101.7052 Da (PGP-B) is connected to the gene leuB pathway, responsible to code 3-isopropylmalate dehydrogenase described for P. dendritiformis C454, P. selenitireducens, P. yonginensis and P. amylolyticus. The enzyme is part of leucine biosynthetic pathway and is responsible to catalyze the oxidation of 3-isopropylmalate. Molecular networking analysis also indicates that the enzyme responsible to synthesize O-acetyl-L-homoserine (gene metAA) expressed by Lysinibacillus sp. strain FJAT-14222 is connected to precursor mass 416.7368 Da. Among QS signaling pathways, N-acyl-homoserine lactones occur as signaling molecules for more than 70 different species of diderm bacteria. QS communication can be interrupted by reducing the activity of signaling receptor proteins, their synthesis or by synthetic molecules analogous to QS signaling compounds, increasing the strategies on fighting against multidrug resistant microorganisms. Notwithstanding, a better understanding of the overlap between system biology and advanced molecular network techniques is needed.
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-06-17T19:47:29Z
dc.date.issued.fl_str_mv 2019-03-25
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
status_str publishedVersion
format doctoralThesis
dc.identifier.citation.fl_str_mv ORTEGA, Daniel Barros. Production of Polypeptin A analogues by Paenibacillus elgii strain AC13 and quorum sensing dependent bacterial processes. 2019. 126 f. Tese (Programa Stricto Sensu em Ci??ncias Gen??micas e Biotecnologia) - Universidade Cat??lica de Bras??lia, Bras??lia, 2019.
dc.identifier.uri.fl_str_mv https://bdtd.ucb.br:8443/jspui/handle/tede/2603
identifier_str_mv ORTEGA, Daniel Barros. Production of Polypeptin A analogues by Paenibacillus elgii strain AC13 and quorum sensing dependent bacterial processes. 2019. 126 f. Tese (Programa Stricto Sensu em Ci??ncias Gen??micas e Biotecnologia) - Universidade Cat??lica de Bras??lia, Bras??lia, 2019.
url https://bdtd.ucb.br:8443/jspui/handle/tede/2603
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Cat??lica de Bras??lia
dc.publisher.program.fl_str_mv Programa Stricto Sensu em Ci??ncias Gen??micas e Biotecnologia
dc.publisher.initials.fl_str_mv UCB
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Escola de Sa??de e Medicina
publisher.none.fl_str_mv Universidade Cat??lica de Bras??lia
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações da UCB
instname:Universidade Católica de Brasília (UCB)
instacron:UCB
instname_str Universidade Católica de Brasília (UCB)
instacron_str UCB
institution UCB
reponame_str Biblioteca Digital de Teses e Dissertações da UCB
collection Biblioteca Digital de Teses e Dissertações da UCB
bitstream.url.fl_str_mv https://200.214.135.178:8443/jspui/bitstream/tede/2603/1/license.txt
https://200.214.135.178:8443/jspui/bitstream/tede/2603/2/DanielBarrosOrtegaTese2019.pdf
https://200.214.135.178:8443/jspui/bitstream/tede/2603/3/DanielBarrosOrtegaTese2019.pdf.txt
https://200.214.135.178:8443/jspui/bitstream/tede/2603/4/DanielBarrosOrtegaTese2019.pdf.jpg
bitstream.checksum.fl_str_mv d7d5e5ec75089f122abe937645a56120
c59ff4cc6eb4e452396e9ee3d1371beb
70e9c6b79911d93ac941e79939d48b68
d9a2e6d73d09d21626810226de388f37
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1724829779831554048

Registros relacionados