Avalia????o genot??pica e fenot??pica de cepas de Staphylococcus ssp. isoladas em hospitais do Distrito Federal no per??odo de 2009 a 2016
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UCB |
Texto Completo: | https://bdtd.ucb.br:8443/jspui/handle/tede/2562 |
Resumo: | The genus Staphylococcus has 36 species and several subspecies, being S. aureus, species of greater clinical interest. For even if it belongs to the human's microbiota, this microorganism, when it can penetrate the tissues due to trauma, injury or low immunity, can develop asymptomatic infections or even serious infections. The vast majority of strains of this genus have resistance against the most different types of antibiotics, which has become a concern from the clinical and control point of these bacteria. Methicillin-resistant strains are called MRSA and can become multi-resistant to other antibiotics, making treatment difficult and costly. The phenotypic and genotypic characterization of the strains is of paramount importance for the knowledge of resistance genes and pathogenicity molecules in order to aid the control as well as the cost of the treatment. In this work, 31 strains of Staphylococcus ssp. belonging to the bacterioteca of the Central Laboratory of Public Health of the Federal District. The phenotypic characterization was performed in a manual and automated way, being carried out tests for identification of the species through analysis of biochemical tests and characterization of resistance profile by antibiogram. The genotypic characterization was performed using molecular tests by multiplex PCR, where the presence of mecA and lukF/S genes was analyzed. For the strains whose result was positive for the mecA gene, the staphylococcal chromosome cassettes (SCCmec) were typed. The results of this study confirm the conclusions made by other authors, where they observed that MRSA strains in addition to being resistant to beta-lactams, showed resistance to other antibiotics (eg clindamycin, gentamicin, ciprofloxacin and sulfamethoxazole / trimethoprim). In this study, 72% of the mecA-positive strains (MRSA and SCN) were identified as SCCmec I or II, suggesting that these infections were contracted in the hospital environment, and 28% of MRSA strains were identified as SCCmec type IV, suggesting that such infections originate in the Community environment. The lukF / S gene was present in 3 samples, all containing the SCCmec IV, confirming the studies of several authors, reporting the production of PVL in strains of community origin. |
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Dias, Simoni Camposhttp://lattes.cnpq.br/1564224041415405http://lattes.cnpq.br/4189185040776284Ara??jo, R??mulo Leles da Silva2019-05-20T19:10:10Z2019-02-28ARA??JO, R??mulo Leles da Silva. Avalia????o genot??pica e fenot??pica de cepas de Staphylococcus ssp. isoladas em hospitais do Distrito Federal no per??odo de 2009 a 2016. 2019. 69 f. Disserta????o (Programa Stricto Sensu em Ci??ncias Gen??micas e Biotecnologia) - Universidade Cat??lica de Bras??lia, Bras??lia, 2019.https://bdtd.ucb.br:8443/jspui/handle/tede/2562The genus Staphylococcus has 36 species and several subspecies, being S. aureus, species of greater clinical interest. For even if it belongs to the human's microbiota, this microorganism, when it can penetrate the tissues due to trauma, injury or low immunity, can develop asymptomatic infections or even serious infections. The vast majority of strains of this genus have resistance against the most different types of antibiotics, which has become a concern from the clinical and control point of these bacteria. Methicillin-resistant strains are called MRSA and can become multi-resistant to other antibiotics, making treatment difficult and costly. The phenotypic and genotypic characterization of the strains is of paramount importance for the knowledge of resistance genes and pathogenicity molecules in order to aid the control as well as the cost of the treatment. In this work, 31 strains of Staphylococcus ssp. belonging to the bacterioteca of the Central Laboratory of Public Health of the Federal District. The phenotypic characterization was performed in a manual and automated way, being carried out tests for identification of the species through analysis of biochemical tests and characterization of resistance profile by antibiogram. The genotypic characterization was performed using molecular tests by multiplex PCR, where the presence of mecA and lukF/S genes was analyzed. For the strains whose result was positive for the mecA gene, the staphylococcal chromosome cassettes (SCCmec) were typed. The results of this study confirm the conclusions made by other authors, where they observed that MRSA strains in addition to being resistant to beta-lactams, showed resistance to other antibiotics (eg clindamycin, gentamicin, ciprofloxacin and sulfamethoxazole / trimethoprim). In this study, 72% of the mecA-positive strains (MRSA and SCN) were identified as SCCmec I or II, suggesting that these infections were contracted in the hospital environment, and 28% of MRSA strains were identified as SCCmec type IV, suggesting that such infections originate in the Community environment. The lukF / S gene was present in 3 samples, all containing the SCCmec IV, confirming the studies of several authors, reporting the production of PVL in strains of community origin.O g??nero Staphylococcus possui 36 esp??cies e v??rias subesp??cies, sendo o S. aureus, esp??cie de maior interesse cl??nico. Mesmo sendo pertencente a microbiota do ser humano, este microrganismo quando consegue penetrar nos tecidos devido a traumas, les??es ou baixa imunidade pode desenvolver infec????es assintom??ticas ou at?? mesmo infec????es grav??ssimas. A maioria das cepas deste g??nero possui resist??ncia contra os mais diferentes tipos de antibi??ticos, o que tem se tornado uma preocupa????o do ponto de vista cl??nico e de controle destas bact??rias. As cepas resistentes ?? meticilina, s??o denominadas como MRSA, podendo se tornar multirresistente a outros antibi??ticos, tornando o tratamento dif??cil e oneroso. A caracteriza????o fenot??pica e genot??pica das cepas ?? de suma import??ncia para o conhecimento dos genes de resist??ncia e virul??ncia com o intuito de auxiliar o controle assim como o custo do tratamento. Neste trabalho foram caracterizadas 31 cepas de Staphylococcus ssp. pertencentes ?? bacterioteca do Laborat??rio Central de Sa??de P??blica do Distrito Federal. A caracteriza????o fenot??pica foi realizada de forma manual e automatizada, sendo realizados ensaios para identifica????o da esp??cie por meio da an??lise de testes bioqu??micos e a caracteriza????o do perfil de resist??ncia por antibiograma. A caracteriza????o genot??pica foi realizada utilizando testes moleculares por PCR multiplex, que analisou a presen??a dos genes mecA e lukF/S. Para as cepas cujo resultado foi positivo para o gene mecA, foi realizada a tipifica????o dos cassetes cromoss??micos estafiloc??cico (SCCmec). Nesta pesquisa, 72% das cepas mecA-positivo (MRSA e SCN), foram identificadas como SCCmec I ou II, sugerindo-se que estas infec????es foram contra??das no ambiente hospitalar, e 28% das cepas MRSA foram identificadas como SCCmec tipo IV, propondo que tais infec????es t??m origem no ambiente comunit??rio. O gene lukF/S esteve presente em 3 amostras pesquisadas, todas contendo o SCCmec IV.Submitted by Sara Ribeiro (sara.ribeiro@ucb.br) on 2019-05-20T19:09:59Z No. of bitstreams: 1 RomuloLelesdaSilvaAraujoDissertacao2019.pdf: 1442848 bytes, checksum: 07322e78c12a6b86f0287b7090733417 (MD5)Approved for entry into archive by Sara Ribeiro (sara.ribeiro@ucb.br) on 2019-05-20T19:10:10Z (GMT) No. of bitstreams: 1 RomuloLelesdaSilvaAraujoDissertacao2019.pdf: 1442848 bytes, checksum: 07322e78c12a6b86f0287b7090733417 (MD5)Made available in DSpace on 2019-05-20T19:10:10Z (GMT). No. of bitstreams: 1 RomuloLelesdaSilvaAraujoDissertacao2019.pdf: 1442848 bytes, checksum: 07322e78c12a6b86f0287b7090733417 (MD5) Previous issue date: 2019-02-28application/pdfhttps://bdtd.ucb.br:8443/jspui/retrieve/6356/RomuloLelesdaSilvaAraujoDissertacao2019.pdf.jpgporUniversidade Cat??lica de Bras??liaPrograma Stricto Sensu em Ci??ncias Gen??micas e BiotecnologiaUCBBrasilEscola de Sa??de e MedicinaMRSAStaphylococcusCaracteriza????o genot??picaGenotypic characterizationCNPQ::CIENCIAS BIOLOGICASAvalia????o genot??pica e fenot??pica de cepas de Staphylococcus ssp. isoladas em hospitais do Distrito Federal no per??odo de 2009 a 2016info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UCBinstname:Universidade Católica de Brasília (UCB)instacron:UCBLICENSElicense.txtlicense.txttext/plain; charset=utf-81905https://200.214.135.178:8443/jspui/bitstream/tede/2562/1/license.txt75558dcf859532757239878b42f1c2c7MD51ORIGINALRomuloLelesdaSilvaAraujoDissertacao2019.pdfRomuloLelesdaSilvaAraujoDissertacao2019.pdfapplication/pdf1442848https://200.214.135.178:8443/jspui/bitstream/tede/2562/2/RomuloLelesdaSilvaAraujoDissertacao2019.pdf07322e78c12a6b86f0287b7090733417MD52TEXTRomuloLelesdaSilvaAraujoDissertacao2019.pdf.txtRomuloLelesdaSilvaAraujoDissertacao2019.pdf.txttext/plain133515https://200.214.135.178:8443/jspui/bitstream/tede/2562/3/RomuloLelesdaSilvaAraujoDissertacao2019.pdf.txt0b17cc892347d54ef0f29e6e58e688a4MD53THUMBNAILRomuloLelesdaSilvaAraujoDissertacao2019.pdf.jpgRomuloLelesdaSilvaAraujoDissertacao2019.pdf.jpgimage/jpeg5115https://200.214.135.178:8443/jspui/bitstream/tede/2562/4/RomuloLelesdaSilvaAraujoDissertacao2019.pdf.jpgbbfc37f5561f583dae62ef99a316676fMD54tede/25622019-05-21 01:06:18.125TElDRU4/P0EgREUgRElTVFJJQlVJPz8/P08gTj8/Ty1FWENMVVNJVkEKCkNvbSBhIGFwcmVzZW50YT8/Pz9vIGRlc3RhIGxpY2VuPz9hLCB2b2M/PyAoYXV0b3Igb3UgbyB0aXR1bGFyIGRvcyBkaXJlaXRvcyBkZSBhdXRvcikgY29uY2VkZSA/PyBVbml2ZXJzaWRhZGUgQ2F0Pz9saWNhIGRlIEJyYXM/P2xpYSAoVUNCKSBvIGRpcmVpdG8gbj8/by1leGNsdXNpdm8gZGUgcmVwcm9kdXppciwgdHJhZHV6aXIgKGNvbmZvcm1lIGRlZmluaWRvIGFiYWl4byksIGUvb3UgZGlzdHJpYnVpciBhIHN1YSB0ZXNlIG91IGRpc3NlcnRhPz8/P28gKGluY2x1aW5kbyBvIHJlc3VtbykgcG9yIHRvZG8gbyBtdW5kbyBubyBmb3JtYXRvIGltcHJlc3NvIGUgZWxldHI/P25pY28gZSBlbSBxdWFscXVlciBtZWlvLCBpbmNsdWluZG8gb3MgZm9ybWF0b3MgPz91ZGlvIG91IHY/P2Rlby4KClZvYz8/IGNvbmNvcmRhIHF1ZSBhIFVDQiBwb2RlLCBzZW0gYWx0ZXJhciBvIGNvbnRlPz9kbywgdHJhbnNwb3IgYSBzdWEgdGVzZSBvdSBkaXNzZXJ0YT8/Pz9vIHBhcmEgcXVhbHF1ZXIgbWVpbyBvdSBmb3JtYXRvIHBhcmEgZmlucyBkZSBwcmVzZXJ2YT8/Pz9vLgoKVm9jPz8gdGFtYj8/bSBjb25jb3JkYSBxdWUgYSBVQ0IgcG9kZSBtYW50ZXIgbWFpcyBkZSB1bWEgYz8/cGlhIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGE/Pz8/byBwYXJhIGZpbnMgZGUgc2VndXJhbj8/YSwgYmFjay11cCBlIHByZXNlcnZhPz8/P28uCgpWb2M/PyBkZWNsYXJhIHF1ZSBhIHN1YSB0ZXNlIG91IGRpc3NlcnRhPz8/P28gPz8gb3JpZ2luYWwgZSBxdWUgdm9jPz8gdGVtIG8gcG9kZXIgZGUgY29uY2VkZXIgb3MgZGlyZWl0b3MgY29udGlkb3MgbmVzdGEgbGljZW4/P2EuIFZvYz8/IHRhbWI/P20gZGVjbGFyYSBxdWUgbyBkZXA/P3NpdG8gZGEgc3VhIHRlc2Ugb3UgZGlzc2VydGE/Pz8/byBuPz9vIGluZnJpbmdlIGRpcmVpdG9zIGF1dG9yYWlzIGRlIG5pbmd1Pz9tLgoKQ2FzbyBhIHN1YSB0ZXNlIG91IGRpc3NlcnRhPz8/P28gY29udGVuaGEgbWF0ZXJpYWwgcXVlIHZvYz8/IG4/P28gcG9zc3VpIGEgdGl0dWxhcmlkYWRlIGRvcyBkaXJlaXRvcyBhdXRvcmFpcywgdm9jPz8gZGVjbGFyYSBxdWUgb2J0ZXZlIGEgcGVybWlzcz8/byBpcnJlc3RyaXRhIGRvIGRldGVudG9yIGRvcyBkaXJlaXRvcyBhdXRvcmFpcyBwYXJhIGNvbmNlZGVyID8/IFVDQiBvcyBkaXJlaXRvcyBhcHJlc2VudGFkb3MgbmVzdGEgbGljZW4/P2EsIGUgcXVlIGVzc2UgbWF0ZXJpYWwgZGUgcHJvcHJpZWRhZGUgZGUgdGVyY2Vpcm9zIGVzdD8/IGNsYXJhbWVudGUgaWRlbnRpZmljYWRvIGUgcmVjb25oZWNpZG8gbm8gdGV4dG8gb3Ugbm8gY29udGU/P2RvIGRhIHRlc2Ugb3UgZGlzc2VydGE/Pz8/byBvcmEgZGVwb3NpdGFkYS4KCkNhc28gYSB0ZXNlIG91IGRpc3NlcnRhPz8/P28gZGVwb3NpdGFkYSB0ZW5oYSBzaWRvIHJlc3VsdGFkbyBkZSB1bSBwYXRyb2M/P25pbyBvdSBhcG9pbyBkZSB1bWEgYWc/P25jaWEgZGUgZm9tZW50byBvdSBvdXRybyBvcmdhbmlzbW8gcXVlIG4/P28gc2VqYSBhIFVDQiwgdm9jPz8gZGVjbGFyYSBxdWUgcmVzcGVpdG91IHRvZG9zIGUgcXVhaXNxdWVyIGRpcmVpdG9zIGRlIHJldmlzPz9vIGNvbW8gdGFtYj8/bSBhcyBkZW1haXMgb2JyaWdhPz8/P2VzIGV4aWdpZGFzIHBvciBjb250cmF0byBvdSBhY29yZG8uCgpBIFVDQiBzZSBjb21wcm9tZXRlIGEgaWRlbnRpZmljYXIgY2xhcmFtZW50ZSBvIHNldSBub21lIChzKSBvdSBvKHMpIG5vbWUocykgZG8ocykgZGV0ZW50b3IoZXMpIGRvcyBkaXJlaXRvcyBhdXRvcmFpcyBkYSB0ZXNlIG91IGRpc3NlcnRhPz8/P28sIGUgbj8/byBmYXI/PyBxdWFscXVlciBhbHRlcmE/Pz8/bywgYWw/P20gZGFxdWVsYXMgY29uY2VkaWRhcyBwb3IgZXN0YSBsaWNlbj8/YS4KBiblioteca Digital de Teses e Dissertaçõeshttps://bdtd.ucb.br:8443/jspui/ |
dc.title.por.fl_str_mv |
Avalia????o genot??pica e fenot??pica de cepas de Staphylococcus ssp. isoladas em hospitais do Distrito Federal no per??odo de 2009 a 2016 |
title |
Avalia????o genot??pica e fenot??pica de cepas de Staphylococcus ssp. isoladas em hospitais do Distrito Federal no per??odo de 2009 a 2016 |
spellingShingle |
Avalia????o genot??pica e fenot??pica de cepas de Staphylococcus ssp. isoladas em hospitais do Distrito Federal no per??odo de 2009 a 2016 Ara??jo, R??mulo Leles da Silva MRSA Staphylococcus Caracteriza????o genot??pica Genotypic characterization CNPQ::CIENCIAS BIOLOGICAS |
title_short |
Avalia????o genot??pica e fenot??pica de cepas de Staphylococcus ssp. isoladas em hospitais do Distrito Federal no per??odo de 2009 a 2016 |
title_full |
Avalia????o genot??pica e fenot??pica de cepas de Staphylococcus ssp. isoladas em hospitais do Distrito Federal no per??odo de 2009 a 2016 |
title_fullStr |
Avalia????o genot??pica e fenot??pica de cepas de Staphylococcus ssp. isoladas em hospitais do Distrito Federal no per??odo de 2009 a 2016 |
title_full_unstemmed |
Avalia????o genot??pica e fenot??pica de cepas de Staphylococcus ssp. isoladas em hospitais do Distrito Federal no per??odo de 2009 a 2016 |
title_sort |
Avalia????o genot??pica e fenot??pica de cepas de Staphylococcus ssp. isoladas em hospitais do Distrito Federal no per??odo de 2009 a 2016 |
author |
Ara??jo, R??mulo Leles da Silva |
author_facet |
Ara??jo, R??mulo Leles da Silva |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Dias, Simoni Campos |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/1564224041415405 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/4189185040776284 |
dc.contributor.author.fl_str_mv |
Ara??jo, R??mulo Leles da Silva |
contributor_str_mv |
Dias, Simoni Campos |
dc.subject.por.fl_str_mv |
MRSA Staphylococcus Caracteriza????o genot??pica |
topic |
MRSA Staphylococcus Caracteriza????o genot??pica Genotypic characterization CNPQ::CIENCIAS BIOLOGICAS |
dc.subject.eng.fl_str_mv |
Genotypic characterization |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS |
dc.description.abstract.eng.fl_txt_mv |
The genus Staphylococcus has 36 species and several subspecies, being S. aureus, species of greater clinical interest. For even if it belongs to the human's microbiota, this microorganism, when it can penetrate the tissues due to trauma, injury or low immunity, can develop asymptomatic infections or even serious infections. The vast majority of strains of this genus have resistance against the most different types of antibiotics, which has become a concern from the clinical and control point of these bacteria. Methicillin-resistant strains are called MRSA and can become multi-resistant to other antibiotics, making treatment difficult and costly. The phenotypic and genotypic characterization of the strains is of paramount importance for the knowledge of resistance genes and pathogenicity molecules in order to aid the control as well as the cost of the treatment. In this work, 31 strains of Staphylococcus ssp. belonging to the bacterioteca of the Central Laboratory of Public Health of the Federal District. The phenotypic characterization was performed in a manual and automated way, being carried out tests for identification of the species through analysis of biochemical tests and characterization of resistance profile by antibiogram. The genotypic characterization was performed using molecular tests by multiplex PCR, where the presence of mecA and lukF/S genes was analyzed. For the strains whose result was positive for the mecA gene, the staphylococcal chromosome cassettes (SCCmec) were typed. The results of this study confirm the conclusions made by other authors, where they observed that MRSA strains in addition to being resistant to beta-lactams, showed resistance to other antibiotics (eg clindamycin, gentamicin, ciprofloxacin and sulfamethoxazole / trimethoprim). In this study, 72% of the mecA-positive strains (MRSA and SCN) were identified as SCCmec I or II, suggesting that these infections were contracted in the hospital environment, and 28% of MRSA strains were identified as SCCmec type IV, suggesting that such infections originate in the Community environment. The lukF / S gene was present in 3 samples, all containing the SCCmec IV, confirming the studies of several authors, reporting the production of PVL in strains of community origin. |
dc.description.abstract.por.fl_txt_mv |
O g??nero Staphylococcus possui 36 esp??cies e v??rias subesp??cies, sendo o S. aureus, esp??cie de maior interesse cl??nico. Mesmo sendo pertencente a microbiota do ser humano, este microrganismo quando consegue penetrar nos tecidos devido a traumas, les??es ou baixa imunidade pode desenvolver infec????es assintom??ticas ou at?? mesmo infec????es grav??ssimas. A maioria das cepas deste g??nero possui resist??ncia contra os mais diferentes tipos de antibi??ticos, o que tem se tornado uma preocupa????o do ponto de vista cl??nico e de controle destas bact??rias. As cepas resistentes ?? meticilina, s??o denominadas como MRSA, podendo se tornar multirresistente a outros antibi??ticos, tornando o tratamento dif??cil e oneroso. A caracteriza????o fenot??pica e genot??pica das cepas ?? de suma import??ncia para o conhecimento dos genes de resist??ncia e virul??ncia com o intuito de auxiliar o controle assim como o custo do tratamento. Neste trabalho foram caracterizadas 31 cepas de Staphylococcus ssp. pertencentes ?? bacterioteca do Laborat??rio Central de Sa??de P??blica do Distrito Federal. A caracteriza????o fenot??pica foi realizada de forma manual e automatizada, sendo realizados ensaios para identifica????o da esp??cie por meio da an??lise de testes bioqu??micos e a caracteriza????o do perfil de resist??ncia por antibiograma. A caracteriza????o genot??pica foi realizada utilizando testes moleculares por PCR multiplex, que analisou a presen??a dos genes mecA e lukF/S. Para as cepas cujo resultado foi positivo para o gene mecA, foi realizada a tipifica????o dos cassetes cromoss??micos estafiloc??cico (SCCmec). Nesta pesquisa, 72% das cepas mecA-positivo (MRSA e SCN), foram identificadas como SCCmec I ou II, sugerindo-se que estas infec????es foram contra??das no ambiente hospitalar, e 28% das cepas MRSA foram identificadas como SCCmec tipo IV, propondo que tais infec????es t??m origem no ambiente comunit??rio. O gene lukF/S esteve presente em 3 amostras pesquisadas, todas contendo o SCCmec IV. |
description |
The genus Staphylococcus has 36 species and several subspecies, being S. aureus, species of greater clinical interest. For even if it belongs to the human's microbiota, this microorganism, when it can penetrate the tissues due to trauma, injury or low immunity, can develop asymptomatic infections or even serious infections. The vast majority of strains of this genus have resistance against the most different types of antibiotics, which has become a concern from the clinical and control point of these bacteria. Methicillin-resistant strains are called MRSA and can become multi-resistant to other antibiotics, making treatment difficult and costly. The phenotypic and genotypic characterization of the strains is of paramount importance for the knowledge of resistance genes and pathogenicity molecules in order to aid the control as well as the cost of the treatment. In this work, 31 strains of Staphylococcus ssp. belonging to the bacterioteca of the Central Laboratory of Public Health of the Federal District. The phenotypic characterization was performed in a manual and automated way, being carried out tests for identification of the species through analysis of biochemical tests and characterization of resistance profile by antibiogram. The genotypic characterization was performed using molecular tests by multiplex PCR, where the presence of mecA and lukF/S genes was analyzed. For the strains whose result was positive for the mecA gene, the staphylococcal chromosome cassettes (SCCmec) were typed. The results of this study confirm the conclusions made by other authors, where they observed that MRSA strains in addition to being resistant to beta-lactams, showed resistance to other antibiotics (eg clindamycin, gentamicin, ciprofloxacin and sulfamethoxazole / trimethoprim). In this study, 72% of the mecA-positive strains (MRSA and SCN) were identified as SCCmec I or II, suggesting that these infections were contracted in the hospital environment, and 28% of MRSA strains were identified as SCCmec type IV, suggesting that such infections originate in the Community environment. The lukF / S gene was present in 3 samples, all containing the SCCmec IV, confirming the studies of several authors, reporting the production of PVL in strains of community origin. |
publishDate |
2019 |
dc.date.accessioned.fl_str_mv |
2019-05-20T19:10:10Z |
dc.date.issued.fl_str_mv |
2019-02-28 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
status_str |
publishedVersion |
format |
masterThesis |
dc.identifier.citation.fl_str_mv |
ARA??JO, R??mulo Leles da Silva. Avalia????o genot??pica e fenot??pica de cepas de Staphylococcus ssp. isoladas em hospitais do Distrito Federal no per??odo de 2009 a 2016. 2019. 69 f. Disserta????o (Programa Stricto Sensu em Ci??ncias Gen??micas e Biotecnologia) - Universidade Cat??lica de Bras??lia, Bras??lia, 2019. |
dc.identifier.uri.fl_str_mv |
https://bdtd.ucb.br:8443/jspui/handle/tede/2562 |
identifier_str_mv |
ARA??JO, R??mulo Leles da Silva. Avalia????o genot??pica e fenot??pica de cepas de Staphylococcus ssp. isoladas em hospitais do Distrito Federal no per??odo de 2009 a 2016. 2019. 69 f. Disserta????o (Programa Stricto Sensu em Ci??ncias Gen??micas e Biotecnologia) - Universidade Cat??lica de Bras??lia, Bras??lia, 2019. |
url |
https://bdtd.ucb.br:8443/jspui/handle/tede/2562 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Cat??lica de Bras??lia |
dc.publisher.program.fl_str_mv |
Programa Stricto Sensu em Ci??ncias Gen??micas e Biotecnologia |
dc.publisher.initials.fl_str_mv |
UCB |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Escola de Sa??de e Medicina |
publisher.none.fl_str_mv |
Universidade Cat??lica de Bras??lia |
dc.source.none.fl_str_mv |
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UCB |
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UCB |
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Biblioteca Digital de Teses e Dissertações da UCB |
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