Variabilidade molecular sazonal de drosophila mediopunctata (diptera: drosophilidae)
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações do UNICENTRO |
Texto Completo: | http://localhost:8080/tede/handle/tede/382 |
Resumo: | Environment fragmentation can result in reduction of biological diversity due to the genetic variability loss through the decline of specimens number in the populations. The decrease in population size can contribute to the population isolation and, therefore, to the restriction of gene flow, which can lead to increase the genetic differentiation among populations and to decrease of genetic variability within population. Accentuated fluctuations in population size can occur in seasonal cycles in response to reproduction ecological specificities linked to resource availability. Drosophila mediopunctata is a species that has a wide geographic distribution in Neotropical Forests and is not associated to humans. The analyses of several genetic markers in natural populations of this species revealed that they are associated with seasonal, altitudinal and latitudinal variation. The aim of this work was to perform a seasonal microsatellite genetic variability analysis in natural populations of D. mediopunctata in order to contribute to the understanding of this species populational dynamics in Araucaria Forest fragments. Eight microsatellite loci were analyzed in 92 individuals collected in two Araucaria Forest areas located in Guarapuava/PR: 60 specimens collected in the Parque Municipal das Araucárias (PMA) (30 in the winter of 2008 and 30 in the spring of 2010), and 32 collected in Guairacá district (GUA) (13 in the spring and 19 in the winter of 2008). It was detected a high genetic diversity and a low populational (Fst= 0.0243) and seasonal (Fst = 0.0249) genetic differentiation. However, the number of exclusive alleles and Nei (1978) genetic distance and identity indicated a certain reproductive isolation among D. mediopunctata populations/subpopulations. The seasonal differentiation in GUA was higher than in PMA (revealed through the Neighbor-Joining analysis) and the within populational variability was higher in PMA than in GUA (Ho = 0.5385 and Ho = 0.5062, respectively). These data allow to suggest that PMA, a conservation park, in spite of having smaller size than GUA, a farm that presents pinion and mate extraction activities, have a more stable population in the genetic point of view due to its higher habitat preservation quality and diversity. |
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Machado, Luciana Paes de BarrosCPF:00000000000http://lattes.cnpq.br/5621542642051269Mateus, Rogério PincelaCPF:00000000000http://lattes.cnpq.br/3619196057484399CPF:06965327944http://lattes.cnpq.br/0701063160346311Heinz, Norbert Padilha2016-09-20T12:29:23Z2014-07-102012-02-01HEINZ, Norbert Padilha. não consta. 2012. 49 f. Dissertação (Mestrado em Biologia Evolutiva) - UNICENTRO - Universidade Estadual do Centro Oeste, Guarapuava, 2012.http://localhost:8080/tede/handle/tede/382Environment fragmentation can result in reduction of biological diversity due to the genetic variability loss through the decline of specimens number in the populations. The decrease in population size can contribute to the population isolation and, therefore, to the restriction of gene flow, which can lead to increase the genetic differentiation among populations and to decrease of genetic variability within population. Accentuated fluctuations in population size can occur in seasonal cycles in response to reproduction ecological specificities linked to resource availability. Drosophila mediopunctata is a species that has a wide geographic distribution in Neotropical Forests and is not associated to humans. The analyses of several genetic markers in natural populations of this species revealed that they are associated with seasonal, altitudinal and latitudinal variation. The aim of this work was to perform a seasonal microsatellite genetic variability analysis in natural populations of D. mediopunctata in order to contribute to the understanding of this species populational dynamics in Araucaria Forest fragments. Eight microsatellite loci were analyzed in 92 individuals collected in two Araucaria Forest areas located in Guarapuava/PR: 60 specimens collected in the Parque Municipal das Araucárias (PMA) (30 in the winter of 2008 and 30 in the spring of 2010), and 32 collected in Guairacá district (GUA) (13 in the spring and 19 in the winter of 2008). It was detected a high genetic diversity and a low populational (Fst= 0.0243) and seasonal (Fst = 0.0249) genetic differentiation. However, the number of exclusive alleles and Nei (1978) genetic distance and identity indicated a certain reproductive isolation among D. mediopunctata populations/subpopulations. The seasonal differentiation in GUA was higher than in PMA (revealed through the Neighbor-Joining analysis) and the within populational variability was higher in PMA than in GUA (Ho = 0.5385 and Ho = 0.5062, respectively). These data allow to suggest that PMA, a conservation park, in spite of having smaller size than GUA, a farm that presents pinion and mate extraction activities, have a more stable population in the genetic point of view due to its higher habitat preservation quality and diversity.A fragmentação de áreas pode resultar na redução da diversidade biológica devido à perda de variabilidade genética por meio da queda no número de indivíduos. A diminuição do tamanho populacional, por sua vez, contribui para o processo de isolamento das populações e consequente restrição do fluxo gênico, acarretando no aumento das diferenças genéticas entre elas e a diminuição da variabilidade intrapopulacional. Flutuações acentuadas no tamanho das populações também podem ocorrer em ciclos sazonais, geralmente por causa de especificidades ecológicas por locais de reprodução, e em resposta a disponibilidade de recursos. Drosophila mediopunctata, objeto deste estudo, é uma espécie com ampla distribuição geográfica nas Florestas Neotropicais, não estando associada com habitats humanos. A análise de diferentes marcadores genéticos em populações naturais de D. mediopunctata revelou a associação destes com variações sazonais, altitudinais e latitudinais. O objetivo deste trabalho foi realizar uma análise sazonal da variabilidade genética de populações de D. mediopunctata, através de loci de microssatélites, para contribuir com o entendimento da dinâmica populacional desta espécie em áreas de Mata de Araucária. Foram analisados oito loci de microssatélites em 92 indivíduos provenientes de áreas de Mata de Araucária da região de Guarapuava/PR: 60 coletados no Parque Municipal das Araucárias (PMA) (30 no inverno de 2008 e 30 na primavera de 2010), e 32 no distrito de Guairacá (GUA) (13 na primavera e 19 no inverno de 2008). Foi observada alta diversidade genética para os loci analisados, baixa diferenciação genética populacional (Fst = 0,0243) e sazonal (Fst = 0,0249), contudo o número de alelos exclusivos, a distância e identidade genéticas de Nei (1978) indicam certo isolamento reprodutivo entre as populações/subpopulações de D. mediopunctata. A diferenciação sazonal em GUA foi maior que em PMA (revelada pela análise de Neighbor-Joining) e a variabilidade intrapopulacional foi maior em PMA que GUA (Ho = 0,5385 e Ho = 0,5062, respectivamente). Estes dados permitem sugerir que PMA, um parque de conservação ambiental, apesar de ser uma área de menor tamanho que GUA, uma fazenda extrativista de pinhão e erva-mate, teria uma população mais estável do ponto de vista genético devido a sua superior qualidade de preservação e diversidade de habitats.Made available in DSpace on 2016-09-20T12:29:23Z (GMT). No. of bitstreams: 1 PR HeinzNP_2012.pdf: 766690 bytes, checksum: c573d05ae3722c91e4c747baf09f5b42 (MD5) Previous issue date: 2012-02-01Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfhttp://localhost:8080/tede/retrieve/1237/PR%20HeinzNP_2012.pdf.jpgporUNICENTRO - Universidade Estadual do Centro OestePrograma de Pós-Graduação em Biologia Evolutiva (Mestrado)UNICENTROBRUnicentro::Departamento de Biologiamicrossatéliteheterozigosidadediversidade genéticamicrosatelliteheterozigositygenetic diversityCIENCIAS BIOLOGICAS::BIOLOGIA GERALVariabilidade molecular sazonal de drosophila mediopunctata (diptera: drosophilidae)não constainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis18315802413433703826006006006006887173787787986718-16345593859312446972075167498588264571info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações do UNICENTROinstname:Universidade Estadual do Centro-Oeste (UNICENTRO)instacron:UNICENTROORIGINALPR HeinzNP_2012.pdfapplication/pdf766690http://localhost:8080/tede/bitstream/tede/382/1/PR+HeinzNP_2012.pdfc573d05ae3722c91e4c747baf09f5b42MD51TEXTPR HeinzNP_2012.pdf.txtPR HeinzNP_2012.pdf.txttext/plain75046http://localhost:8080/tede/bitstream/tede/382/2/PR+HeinzNP_2012.pdf.txt05a4f1a93b21d2133f844bf0a06a8dd8MD52THUMBNAILPR HeinzNP_2012.pdf.jpgPR HeinzNP_2012.pdf.jpgimage/jpeg3139http://localhost:8080/tede/bitstream/tede/382/3/PR+HeinzNP_2012.pdf.jpg598c6794560e139e667f4a1402071654MD53tede/3822017-09-12 08:39:30.705oai:localhost:tede/382Biblioteca Digital de Teses e Dissertaçõeshttp://tede.unicentro.br:8080/jspui/PUBhttp://tede.unicentro.br/tde_oai/oai3.phprepositorio@unicentro.br||fabianoqueiroz@yahoo.com.bropendoar:2017-09-12T11:39:30Biblioteca Digital de Teses e Dissertações do UNICENTRO - Universidade Estadual do Centro-Oeste (UNICENTRO)false |
dc.title.por.fl_str_mv |
Variabilidade molecular sazonal de drosophila mediopunctata (diptera: drosophilidae) |
dc.title.alternative.eng.fl_str_mv |
não consta |
title |
Variabilidade molecular sazonal de drosophila mediopunctata (diptera: drosophilidae) |
spellingShingle |
Variabilidade molecular sazonal de drosophila mediopunctata (diptera: drosophilidae) Heinz, Norbert Padilha microssatélite heterozigosidade diversidade genética microsatellite heterozigosity genetic diversity CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
title_short |
Variabilidade molecular sazonal de drosophila mediopunctata (diptera: drosophilidae) |
title_full |
Variabilidade molecular sazonal de drosophila mediopunctata (diptera: drosophilidae) |
title_fullStr |
Variabilidade molecular sazonal de drosophila mediopunctata (diptera: drosophilidae) |
title_full_unstemmed |
Variabilidade molecular sazonal de drosophila mediopunctata (diptera: drosophilidae) |
title_sort |
Variabilidade molecular sazonal de drosophila mediopunctata (diptera: drosophilidae) |
author |
Heinz, Norbert Padilha |
author_facet |
Heinz, Norbert Padilha |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Machado, Luciana Paes de Barros |
dc.contributor.advisor1ID.fl_str_mv |
CPF:00000000000 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/5621542642051269 |
dc.contributor.advisor-co1.fl_str_mv |
Mateus, Rogério Pincela |
dc.contributor.advisor-co1ID.fl_str_mv |
CPF:00000000000 |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/3619196057484399 |
dc.contributor.authorID.fl_str_mv |
CPF:06965327944 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/0701063160346311 |
dc.contributor.author.fl_str_mv |
Heinz, Norbert Padilha |
contributor_str_mv |
Machado, Luciana Paes de Barros Mateus, Rogério Pincela |
dc.subject.por.fl_str_mv |
microssatélite heterozigosidade diversidade genética |
topic |
microssatélite heterozigosidade diversidade genética microsatellite heterozigosity genetic diversity CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
dc.subject.eng.fl_str_mv |
microsatellite heterozigosity genetic diversity |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
description |
Environment fragmentation can result in reduction of biological diversity due to the genetic variability loss through the decline of specimens number in the populations. The decrease in population size can contribute to the population isolation and, therefore, to the restriction of gene flow, which can lead to increase the genetic differentiation among populations and to decrease of genetic variability within population. Accentuated fluctuations in population size can occur in seasonal cycles in response to reproduction ecological specificities linked to resource availability. Drosophila mediopunctata is a species that has a wide geographic distribution in Neotropical Forests and is not associated to humans. The analyses of several genetic markers in natural populations of this species revealed that they are associated with seasonal, altitudinal and latitudinal variation. The aim of this work was to perform a seasonal microsatellite genetic variability analysis in natural populations of D. mediopunctata in order to contribute to the understanding of this species populational dynamics in Araucaria Forest fragments. Eight microsatellite loci were analyzed in 92 individuals collected in two Araucaria Forest areas located in Guarapuava/PR: 60 specimens collected in the Parque Municipal das Araucárias (PMA) (30 in the winter of 2008 and 30 in the spring of 2010), and 32 collected in Guairacá district (GUA) (13 in the spring and 19 in the winter of 2008). It was detected a high genetic diversity and a low populational (Fst= 0.0243) and seasonal (Fst = 0.0249) genetic differentiation. However, the number of exclusive alleles and Nei (1978) genetic distance and identity indicated a certain reproductive isolation among D. mediopunctata populations/subpopulations. The seasonal differentiation in GUA was higher than in PMA (revealed through the Neighbor-Joining analysis) and the within populational variability was higher in PMA than in GUA (Ho = 0.5385 and Ho = 0.5062, respectively). These data allow to suggest that PMA, a conservation park, in spite of having smaller size than GUA, a farm that presents pinion and mate extraction activities, have a more stable population in the genetic point of view due to its higher habitat preservation quality and diversity. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-02-01 |
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2014-07-10 |
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2016-09-20T12:29:23Z |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
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HEINZ, Norbert Padilha. não consta. 2012. 49 f. Dissertação (Mestrado em Biologia Evolutiva) - UNICENTRO - Universidade Estadual do Centro Oeste, Guarapuava, 2012. |
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http://localhost:8080/tede/handle/tede/382 |
identifier_str_mv |
HEINZ, Norbert Padilha. não consta. 2012. 49 f. Dissertação (Mestrado em Biologia Evolutiva) - UNICENTRO - Universidade Estadual do Centro Oeste, Guarapuava, 2012. |
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