Suplementação com levedura saccharomyces cerevisae reduz contaminação microbiológica da carcaça em novilhos terminados em confinamento

Detalhes bibliográficos
Autor(a) principal: Delazeri, Dailis
Data de Publicação: 2020
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações do UNICENTRO
Texto Completo: http://tede.unicentro.br:8080/jspui/handle/jspui/1790
Resumo: The use of yeast cell wall feed additives (PCL) has already been studied with the aim of favouring the growth of beneficial intestinal bacteria to the detriment of pathogens in ruminants, thus decreasing diarrhoea and increasing animal performance. It is also believed that this change in the intestinal microbiota could contribute to less contamination of the bovine carcass at the time of slaughter, a fact that has not yet been studied in beef cattle. Thus, the present work aims at verifying if the prebiotic cell wall base of Saccharomyces cerevisiae reduces total coliforms, Escherichia coli and Salmonella spp from the feces and the bovine carcass at the time of slaughter after evisceration. For this purpose, 18 steers, ½ Angus blood, confined, were submitted to three daily treatments for 105 days: control (yeast-free diet), 4g yeast (4g/animal/day diet of 2 x 1010cel/g of Saccharomyces cerevisiae commercial RumenYeast®, ICC, São Paulo, Brazil), 7g yeast (7g/animal/day diet of 2 x 1010cel/g of Saccharomyces cerevisiae commercial RumenYeast®, ICC, São Paulo, Brazil). On the 29th and 90th days after entering the confinement, analyzes were made from samples of feces stool, leather, water and food samples were collected for Escherichia coli and total coliforms counting and isolation of Salmonella spp. based on the methodology described by the manufacturer Petrifilm 3M®. On the day of slaughter, the carcasses were collected for identification and quantification of Escherichia coli, total coliforms, mesophils by the same methodology and qualitative identification of Salmonella spp. by ISO 6579/2017 methodology. Reduction of Escherichia coli and total coliforms in stool samples (P=0.0001 and 0.001 respectively) and Escherichia coli, total coliforms and mesophilic aerobes in the carcass (P=0.06; 0.10 and 0.05 respectively) were noted in the groups treated with the two doses of the prebiotic, and that there was absence of Salmonella spp. in all collections performed. It was concluded that supplementation with 4 or 7 g/day of PCL reduced the fecal excretion and consequently the contamination of the carcass by Escherichia coli, mesophils and total coliforms of the confined animals, being a benign and residue-free sanitary measure to improve the microbiological quality of the meat.
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spelling Bertagnon, Heloisa Godoihttp://lattes.cnpq.br/5773536284154541008.238.199-22http://lattes.cnpq.br/0959021659238344Delazeri, Dailis2021-11-26T14:55:02Z2020-03-24Delazeri, Dailis. Suplementação com levedura saccharomyces cerevisae reduz contaminação microbiológica da carcaça em novilhos terminados em confinamento. 2020. 38 f. Dissertação (Programa de Pós-Graduação em Medicina Veterinária - Mestrado) - Universidade Estadual do Centro-Oeste, Guarapuava-PR.http://tede.unicentro.br:8080/jspui/handle/jspui/1790The use of yeast cell wall feed additives (PCL) has already been studied with the aim of favouring the growth of beneficial intestinal bacteria to the detriment of pathogens in ruminants, thus decreasing diarrhoea and increasing animal performance. It is also believed that this change in the intestinal microbiota could contribute to less contamination of the bovine carcass at the time of slaughter, a fact that has not yet been studied in beef cattle. Thus, the present work aims at verifying if the prebiotic cell wall base of Saccharomyces cerevisiae reduces total coliforms, Escherichia coli and Salmonella spp from the feces and the bovine carcass at the time of slaughter after evisceration. For this purpose, 18 steers, ½ Angus blood, confined, were submitted to three daily treatments for 105 days: control (yeast-free diet), 4g yeast (4g/animal/day diet of 2 x 1010cel/g of Saccharomyces cerevisiae commercial RumenYeast®, ICC, São Paulo, Brazil), 7g yeast (7g/animal/day diet of 2 x 1010cel/g of Saccharomyces cerevisiae commercial RumenYeast®, ICC, São Paulo, Brazil). On the 29th and 90th days after entering the confinement, analyzes were made from samples of feces stool, leather, water and food samples were collected for Escherichia coli and total coliforms counting and isolation of Salmonella spp. based on the methodology described by the manufacturer Petrifilm 3M®. On the day of slaughter, the carcasses were collected for identification and quantification of Escherichia coli, total coliforms, mesophils by the same methodology and qualitative identification of Salmonella spp. by ISO 6579/2017 methodology. Reduction of Escherichia coli and total coliforms in stool samples (P=0.0001 and 0.001 respectively) and Escherichia coli, total coliforms and mesophilic aerobes in the carcass (P=0.06; 0.10 and 0.05 respectively) were noted in the groups treated with the two doses of the prebiotic, and that there was absence of Salmonella spp. in all collections performed. It was concluded that supplementation with 4 or 7 g/day of PCL reduced the fecal excretion and consequently the contamination of the carcass by Escherichia coli, mesophils and total coliforms of the confined animals, being a benign and residue-free sanitary measure to improve the microbiological quality of the meat.O uso de aditivos alimentares a base de parede celular de leveduras (PCL) já vem sendo estudo com o intuito de favorecer o crescimento de bactérias benéficas intestinais em detrimento das patogênicas em ruminantes e com isso diminuir quadros de diarreias e aumentar a performance animal. Acredita-se também, que esta alteração na microbiota intestinal, poderia contribuir com uma menor contaminação da carcaça bovina no momento do abate, fato que ainda não foi estudado em bovinos de corte. Deste modo o presente trabalho objetiva verificar se o prebiótico a base de parede celular de Saccharomyces cerevisiae reduz coliformes totais, Escherichia coli e Salmonella spp das fezes e da carcaça bovina no momento do abate após a evisceração. Para tanto, 18 novilhos, ½ sangue Angus, confinados, foram submetidos a três tratamentos diários durante 105 dias: controle (dieta sem leveduras), levedura 4g (dieta com 4g/animal/dia de 2 x 1010cel/g de Saccharomyces cerevisiae comercial RumenYeast®, ICC, São Paulo, Brasil), levedura 7g (dieta com 7g/animal/dia, de 2 x 1010cel/g de Saccharomyces cerevisiae comercial RumenYeast®, ICC, São Paulo, Brasil ). Nos dias 29 e 90 após a entrada no confinamento, fez-se as análises a partir de amostras de fezes, couro, água e alimento para contagem de Escherichia coli e coliformes totais e isolamento de Salmonella spp. baseado na metodologia descrita pelo fabricante Petrifilm 3M®. No dia do abate, realizou-se coleta nas carcaças para identificação e quantificação de Escherichia coli, coliformes totais, mesófilos pela mesma metodologia e identificação qualitativa de Salmonella spp. pela metodologia ISO 6579/2017. Notou-se redução de Escherichia coli e coliformes totais nas amostras de fezes (P=0,0001 e 0,001 respectivamente) e de Escherichia coli, coliformes totais e aeróbios mesófilos na carcaça (P=0,06; 0,10 e 0,05 respectivamente) nos grupos tratados com as duas doses do prebiótico, e que houve ausência de Salmonella spp. em todas as coletas realizadas. Concluiu-se que a suplementação com 4 ou 7 g/dia de PCL reduziu a excreção fecal e consequentemente, a contaminação da carcaça pela Escherichia coli, mesófilos e coliformes totais dos animais confinados, sendo uma medida sanitária benéfíca e livre de resíduos para melhorar a qualidade microbiológica da carne.Submitted by Fabiano Jucá (fjuca@unicentro.br) on 2021-11-26T14:55:02Z No. of bitstreams: 1 Dissertação Dailis Delazeri.pdf: 451316 bytes, checksum: 99f97e88e61d5b02ece6a5224969b1f0 (MD5)Made available in DSpace on 2021-11-26T14:55:02Z (GMT). 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dc.title.por.fl_str_mv Suplementação com levedura saccharomyces cerevisae reduz contaminação microbiológica da carcaça em novilhos terminados em confinamento
dc.title.alternative.eng.fl_str_mv Evaluation of Saccharomyces cerevisiae yeast in the reduction of contamination microbiological of bovine carcasses
title Suplementação com levedura saccharomyces cerevisae reduz contaminação microbiológica da carcaça em novilhos terminados em confinamento
spellingShingle Suplementação com levedura saccharomyces cerevisae reduz contaminação microbiológica da carcaça em novilhos terminados em confinamento
Delazeri, Dailis
levedura
microrganismos
frigorífico
yeast
microorganisms
fridge
CIENCIAS AGRARIAS::MEDICINA VETERINARIA
title_short Suplementação com levedura saccharomyces cerevisae reduz contaminação microbiológica da carcaça em novilhos terminados em confinamento
title_full Suplementação com levedura saccharomyces cerevisae reduz contaminação microbiológica da carcaça em novilhos terminados em confinamento
title_fullStr Suplementação com levedura saccharomyces cerevisae reduz contaminação microbiológica da carcaça em novilhos terminados em confinamento
title_full_unstemmed Suplementação com levedura saccharomyces cerevisae reduz contaminação microbiológica da carcaça em novilhos terminados em confinamento
title_sort Suplementação com levedura saccharomyces cerevisae reduz contaminação microbiológica da carcaça em novilhos terminados em confinamento
author Delazeri, Dailis
author_facet Delazeri, Dailis
author_role author
dc.contributor.advisor1.fl_str_mv Bertagnon, Heloisa Godoi
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/5773536284154541
dc.contributor.authorID.fl_str_mv 008.238.199-22
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/0959021659238344
dc.contributor.author.fl_str_mv Delazeri, Dailis
contributor_str_mv Bertagnon, Heloisa Godoi
dc.subject.por.fl_str_mv levedura
microrganismos
frigorífico
topic levedura
microrganismos
frigorífico
yeast
microorganisms
fridge
CIENCIAS AGRARIAS::MEDICINA VETERINARIA
dc.subject.eng.fl_str_mv yeast
microorganisms
fridge
dc.subject.cnpq.fl_str_mv CIENCIAS AGRARIAS::MEDICINA VETERINARIA
description The use of yeast cell wall feed additives (PCL) has already been studied with the aim of favouring the growth of beneficial intestinal bacteria to the detriment of pathogens in ruminants, thus decreasing diarrhoea and increasing animal performance. It is also believed that this change in the intestinal microbiota could contribute to less contamination of the bovine carcass at the time of slaughter, a fact that has not yet been studied in beef cattle. Thus, the present work aims at verifying if the prebiotic cell wall base of Saccharomyces cerevisiae reduces total coliforms, Escherichia coli and Salmonella spp from the feces and the bovine carcass at the time of slaughter after evisceration. For this purpose, 18 steers, ½ Angus blood, confined, were submitted to three daily treatments for 105 days: control (yeast-free diet), 4g yeast (4g/animal/day diet of 2 x 1010cel/g of Saccharomyces cerevisiae commercial RumenYeast®, ICC, São Paulo, Brazil), 7g yeast (7g/animal/day diet of 2 x 1010cel/g of Saccharomyces cerevisiae commercial RumenYeast®, ICC, São Paulo, Brazil). On the 29th and 90th days after entering the confinement, analyzes were made from samples of feces stool, leather, water and food samples were collected for Escherichia coli and total coliforms counting and isolation of Salmonella spp. based on the methodology described by the manufacturer Petrifilm 3M®. On the day of slaughter, the carcasses were collected for identification and quantification of Escherichia coli, total coliforms, mesophils by the same methodology and qualitative identification of Salmonella spp. by ISO 6579/2017 methodology. Reduction of Escherichia coli and total coliforms in stool samples (P=0.0001 and 0.001 respectively) and Escherichia coli, total coliforms and mesophilic aerobes in the carcass (P=0.06; 0.10 and 0.05 respectively) were noted in the groups treated with the two doses of the prebiotic, and that there was absence of Salmonella spp. in all collections performed. It was concluded that supplementation with 4 or 7 g/day of PCL reduced the fecal excretion and consequently the contamination of the carcass by Escherichia coli, mesophils and total coliforms of the confined animals, being a benign and residue-free sanitary measure to improve the microbiological quality of the meat.
publishDate 2020
dc.date.issued.fl_str_mv 2020-03-24
dc.date.accessioned.fl_str_mv 2021-11-26T14:55:02Z
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dc.identifier.citation.fl_str_mv Delazeri, Dailis. Suplementação com levedura saccharomyces cerevisae reduz contaminação microbiológica da carcaça em novilhos terminados em confinamento. 2020. 38 f. Dissertação (Programa de Pós-Graduação em Medicina Veterinária - Mestrado) - Universidade Estadual do Centro-Oeste, Guarapuava-PR.
dc.identifier.uri.fl_str_mv http://tede.unicentro.br:8080/jspui/handle/jspui/1790
identifier_str_mv Delazeri, Dailis. Suplementação com levedura saccharomyces cerevisae reduz contaminação microbiológica da carcaça em novilhos terminados em confinamento. 2020. 38 f. Dissertação (Programa de Pós-Graduação em Medicina Veterinária - Mestrado) - Universidade Estadual do Centro-Oeste, Guarapuava-PR.
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dc.publisher.department.fl_str_mv Unicentro::Departamento de Medicina Veterinária
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