Desenvolvimento e Caracterização de Nanopartículas Políméricas Contendo Ácido Ferúlico
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações do UNICENTRO |
Texto Completo: | http://tede.unicentro.br:8080/jspui/handle/jspui/1592 |
Resumo: | Ferulic acid (FA) is a phenolic compound belonging to the class of hydroxycinnamic acids. For oral administration has limitations in its therapeutic efficacy. It has low water solubility, reduced ability to penetrate biological membranes and low cell absorption. The use of nanocarrier systems can be an alternative to improve the physicochemical and bioavailability aspects. Two carrier systems with PA were developed, one with casein and one with zein-casein-lysine. Casein nanoparticles were obtained by the desolvation method, while those of zein-casein-lysine were obtained by the liquid-liquid dispersion method. The formulations developed had their mean diameter (DM), polydispersion index (IPD) and Zeta potential determined by Dynamic Light Scattering measurements. For casein nanoparticles, 170 nm DM and 0.26 IPD and Zeta-23 mV potential were obtained, while for zein-casein-lysine system, 200 nm DM and 0.17 IPD and Zeta potential were obtained. -26 mV. The quantification of the FA was performed by high performance liquid chromatography (HPLC) with diode array detector (DAD) obtaining an encapsulation efficiency (EE) of 26% for casein particles and 23% for zein-system. casein-lysine. The obtained particles were characterized by Fourrier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and field emission scanning electron microscopy (SEM-FEG). Photomicrographs confirmed the size and spherical morphology of the particles. The FT-IR did not indicate the formation of new functional groups, while in the diffractograms there were no characteristic diffraction peaks of FA crystal structure, indicating that it was incorporated into the nanostructured system, suggesting the amorphization of the compound. Zein-casein-lysine nanoparticles were also evaluated for cytotoxicity against Caco-2 and HT29-MTX cells, two intestinal strains, to verify their biocompatibility and safety. In addition, Caco-2 monoculture intestinal permeability and Caco-2 / HT29-MTX co-culture assays proved satisfactory for this FA carrier system. |
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Khalil, Najeh Maissarhttp://lattes.cnpq.br/8578241611510102Mainardes, Rubiana Marahttp://lattes.cnpq.br/7632867790178003033.801.199-46http://lattes.cnpq.br/1041944790258972Heep, Graciela Leila2021-04-30T14:28:28Z2019-08-28Heep, Graciela Leila. Desenvolvimento e Caracterização de Nanopartículas Políméricas Contendo Ácido Ferúlico. 2019. 98 f. Tese (Programa de Pós-Graduação em Química - Doutorado) - Universidade Estadual do Centro-Oeste, Guarapuava-PR.http://tede.unicentro.br:8080/jspui/handle/jspui/1592Ferulic acid (FA) is a phenolic compound belonging to the class of hydroxycinnamic acids. For oral administration has limitations in its therapeutic efficacy. It has low water solubility, reduced ability to penetrate biological membranes and low cell absorption. The use of nanocarrier systems can be an alternative to improve the physicochemical and bioavailability aspects. Two carrier systems with PA were developed, one with casein and one with zein-casein-lysine. Casein nanoparticles were obtained by the desolvation method, while those of zein-casein-lysine were obtained by the liquid-liquid dispersion method. The formulations developed had their mean diameter (DM), polydispersion index (IPD) and Zeta potential determined by Dynamic Light Scattering measurements. For casein nanoparticles, 170 nm DM and 0.26 IPD and Zeta-23 mV potential were obtained, while for zein-casein-lysine system, 200 nm DM and 0.17 IPD and Zeta potential were obtained. -26 mV. The quantification of the FA was performed by high performance liquid chromatography (HPLC) with diode array detector (DAD) obtaining an encapsulation efficiency (EE) of 26% for casein particles and 23% for zein-system. casein-lysine. The obtained particles were characterized by Fourrier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and field emission scanning electron microscopy (SEM-FEG). Photomicrographs confirmed the size and spherical morphology of the particles. The FT-IR did not indicate the formation of new functional groups, while in the diffractograms there were no characteristic diffraction peaks of FA crystal structure, indicating that it was incorporated into the nanostructured system, suggesting the amorphization of the compound. Zein-casein-lysine nanoparticles were also evaluated for cytotoxicity against Caco-2 and HT29-MTX cells, two intestinal strains, to verify their biocompatibility and safety. In addition, Caco-2 monoculture intestinal permeability and Caco-2 / HT29-MTX co-culture assays proved satisfactory for this FA carrier system.O ácido ferúlico (AF) é um composto fenólico pertencente à classe dos ácidos hidroxicinâmicos. Para administração via oral possui limitações na sua eficácia terapêutica. Apresenta baixa solubilidade em água, capacidade reduzida de penetrar membranas biológicas e baixa absorção celular. O uso de sistemas nanocarreadores pode ser uma alternativa para melhorar os aspectos físico-químicos e de biodisponibilidade. Foram desenvolvidos dois sistemas carreadores com AF, um com caseína e outro com zeína-caseína-lisina. As nanopartículas de caseína foram obtidas por meio do método de dessolvatação, enquanto as de zeína-caseína-lisina foram obtidas por meio do método de dispersão líquido-líquido. As formulações desenvolvidas tiveram seu diâmetro médio (DM), índice de polidispersão (IPD) e potencial Zeta determinados por meio de medidas realizadas de Espalhamento Dinâmico de Luz. Para as nanopartículas de caseína obtiveram-se DM de 170 nm e IPD de 0,26 e potencial Zeta -23 mV, enquanto para o sitema zeína-caseína-lisina obteve-se DM de 200 nm e IPD de 0,17 e potencial Zeta -26 mV. A quantificação do AF foi realizada por cromatografia líquida de alta eficiência (CLAE) com detector de arranjo de diodo (DAD) obtendo-se uma eficiência de encapsulação (EE) de 26% para as partículas de caseína e 23% para o sistema zeína-caseína-lisina. As partículas obtidas foram caracterizadas por meio de espectroscopia de infravermelho com transformada de Fourrier (FT-IR), difratometria de raios X (DRX) e microscopia eletrônica de varredura com emissão de campo (MEV-FEG). As fotomicrografias confirmaram o tamanho e a morfologia esférica das partículas. O FT-IR não indicou a formação de novos grupos funcionais, enquanto que nos difratogramas não houve picos de difração característicos de estrutura cristalina do AF, indicando que houve uma incorporação deste no sistema nanoestruturado, sugerindo a amorfização do composto. As nanopartículas de zeína-caseína-lisina foram avaliadas, também, quanto a sua citotoxicidade contra células Caco-2 e HT29-MTX, duas linhagens intestinais, para verificar sua biocompatibilidade e segurança. Além disso, ensaios de permeabilidade intestinal monocultura Caco-2 e co-cultura Caco-2/HT29-MTX demonstraram-se satisfatórios para esse sistema carreador de AF.Submitted by Fabiano Jucá (fjuca@unicentro.br) on 2021-04-30T14:28:28Z No. of bitstreams: 1 Tese GRACIELA LEILA HEEP.pdf: 2518428 bytes, checksum: 11def5c48ede061f916e1e089c14df2a (MD5)Made available in DSpace on 2021-04-30T14:28:28Z (GMT). No. of bitstreams: 1 Tese GRACIELA LEILA HEEP.pdf: 2518428 bytes, checksum: 11def5c48ede061f916e1e089c14df2a (MD5) Previous issue date: 2019-08-28Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfhttp://tede.unicentro.br:8080/jspui/retrieve/6469/Tese%20GRACIELA%20LEILA%20HEEP.pdf.jpghttp://tede.unicentro.br:8080/jspui/retrieve/6471/Tese%20GRACIELA%20LEILA%20HEEP.pdf.jpgporUniversidade Estadual do Centro-OestePrograma de Pós-Graduação em Química (Doutorado)UNICENTROBrasilUnicentro::Departamento de Ciências Exatas e de TecnologiaNanopartículasproteínasmodelos celularescitotoxicidadeácido ferúlicoNanoparticlesproteinscellular modelscytotoxicityferulic acidCIENCIAS EXATAS E DA TERRA::QUIMICADesenvolvimento e Caracterização de Nanopartículas Políméricas Contendo Ácido FerúlicoDevelopment and Characterization of Polymeric Nanoparticles Containing Ferulic Acidinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis3800526532796635565600600600600-433806549027752903315717003253031171952075167498588264571info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações do UNICENTROinstname:Universidade Estadual do Centro-Oeste (UNICENTRO)instacron:UNICENTROTHUMBNAILTese GRACIELA LEILA HEEP.pdf.jpgTese GRACIELA LEILA HEEP.pdf.jpgimage/jpeg1943http://localhost:8080/tede/bitstream/jspui/1592/4/Tese+GRACIELA+LEILA+HEEP.pdf.jpgcc73c4c239a4c332d642ba1e7c7a9fb2MD54TEXTTese GRACIELA LEILA HEEP.pdf.txtTese GRACIELA LEILA HEEP.pdf.txttext/plain179056http://localhost:8080/tede/bitstream/jspui/1592/3/Tese+GRACIELA+LEILA+HEEP.pdf.txtcbae18cf2f92cfdf4948067001454376MD53THUMBNAILTese GRACIELA LEILA HEEP.pdf.jpgTese GRACIELA LEILA HEEP.pdf.jpgimage/jpeg1943http://localhost:8080/tede/bitstream/jspui/1592/4/Tese+GRACIELA+LEILA+HEEP.pdf.jpgcc73c4c239a4c332d642ba1e7c7a9fb2MD54TEXTTese GRACIELA LEILA HEEP.pdf.txtTese GRACIELA LEILA HEEP.pdf.txttext/plain179056http://localhost:8080/tede/bitstream/jspui/1592/3/Tese+GRACIELA+LEILA+HEEP.pdf.txtcbae18cf2f92cfdf4948067001454376MD53ORIGINALTese GRACIELA LEILA HEEP.pdfTese GRACIELA LEILA HEEP.pdfapplication/pdf2518428http://localhost:8080/tede/bitstream/jspui/1592/2/Tese+GRACIELA+LEILA+HEEP.pdf11def5c48ede061f916e1e089c14df2aMD52LICENSElicense.txtlicense.txttext/plain; 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dc.title.por.fl_str_mv |
Desenvolvimento e Caracterização de Nanopartículas Políméricas Contendo Ácido Ferúlico |
dc.title.alternative.eng.fl_str_mv |
Development and Characterization of Polymeric Nanoparticles Containing Ferulic Acid |
title |
Desenvolvimento e Caracterização de Nanopartículas Políméricas Contendo Ácido Ferúlico |
spellingShingle |
Desenvolvimento e Caracterização de Nanopartículas Políméricas Contendo Ácido Ferúlico Heep, Graciela Leila Nanopartículas proteínas modelos celulares citotoxicidade ácido ferúlico Nanoparticles proteins cellular models cytotoxicity ferulic acid CIENCIAS EXATAS E DA TERRA::QUIMICA |
title_short |
Desenvolvimento e Caracterização de Nanopartículas Políméricas Contendo Ácido Ferúlico |
title_full |
Desenvolvimento e Caracterização de Nanopartículas Políméricas Contendo Ácido Ferúlico |
title_fullStr |
Desenvolvimento e Caracterização de Nanopartículas Políméricas Contendo Ácido Ferúlico |
title_full_unstemmed |
Desenvolvimento e Caracterização de Nanopartículas Políméricas Contendo Ácido Ferúlico |
title_sort |
Desenvolvimento e Caracterização de Nanopartículas Políméricas Contendo Ácido Ferúlico |
author |
Heep, Graciela Leila |
author_facet |
Heep, Graciela Leila |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Khalil, Najeh Maissar |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/8578241611510102 |
dc.contributor.advisor-co1.fl_str_mv |
Mainardes, Rubiana Mara |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/7632867790178003 |
dc.contributor.authorID.fl_str_mv |
033.801.199-46 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/1041944790258972 |
dc.contributor.author.fl_str_mv |
Heep, Graciela Leila |
contributor_str_mv |
Khalil, Najeh Maissar Mainardes, Rubiana Mara |
dc.subject.por.fl_str_mv |
Nanopartículas proteínas modelos celulares citotoxicidade ácido ferúlico |
topic |
Nanopartículas proteínas modelos celulares citotoxicidade ácido ferúlico Nanoparticles proteins cellular models cytotoxicity ferulic acid CIENCIAS EXATAS E DA TERRA::QUIMICA |
dc.subject.eng.fl_str_mv |
Nanoparticles proteins cellular models cytotoxicity ferulic acid |
dc.subject.cnpq.fl_str_mv |
CIENCIAS EXATAS E DA TERRA::QUIMICA |
description |
Ferulic acid (FA) is a phenolic compound belonging to the class of hydroxycinnamic acids. For oral administration has limitations in its therapeutic efficacy. It has low water solubility, reduced ability to penetrate biological membranes and low cell absorption. The use of nanocarrier systems can be an alternative to improve the physicochemical and bioavailability aspects. Two carrier systems with PA were developed, one with casein and one with zein-casein-lysine. Casein nanoparticles were obtained by the desolvation method, while those of zein-casein-lysine were obtained by the liquid-liquid dispersion method. The formulations developed had their mean diameter (DM), polydispersion index (IPD) and Zeta potential determined by Dynamic Light Scattering measurements. For casein nanoparticles, 170 nm DM and 0.26 IPD and Zeta-23 mV potential were obtained, while for zein-casein-lysine system, 200 nm DM and 0.17 IPD and Zeta potential were obtained. -26 mV. The quantification of the FA was performed by high performance liquid chromatography (HPLC) with diode array detector (DAD) obtaining an encapsulation efficiency (EE) of 26% for casein particles and 23% for zein-system. casein-lysine. The obtained particles were characterized by Fourrier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and field emission scanning electron microscopy (SEM-FEG). Photomicrographs confirmed the size and spherical morphology of the particles. The FT-IR did not indicate the formation of new functional groups, while in the diffractograms there were no characteristic diffraction peaks of FA crystal structure, indicating that it was incorporated into the nanostructured system, suggesting the amorphization of the compound. Zein-casein-lysine nanoparticles were also evaluated for cytotoxicity against Caco-2 and HT29-MTX cells, two intestinal strains, to verify their biocompatibility and safety. In addition, Caco-2 monoculture intestinal permeability and Caco-2 / HT29-MTX co-culture assays proved satisfactory for this FA carrier system. |
publishDate |
2019 |
dc.date.issued.fl_str_mv |
2019-08-28 |
dc.date.accessioned.fl_str_mv |
2021-04-30T14:28:28Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Heep, Graciela Leila. Desenvolvimento e Caracterização de Nanopartículas Políméricas Contendo Ácido Ferúlico. 2019. 98 f. Tese (Programa de Pós-Graduação em Química - Doutorado) - Universidade Estadual do Centro-Oeste, Guarapuava-PR. |
dc.identifier.uri.fl_str_mv |
http://tede.unicentro.br:8080/jspui/handle/jspui/1592 |
identifier_str_mv |
Heep, Graciela Leila. Desenvolvimento e Caracterização de Nanopartículas Políméricas Contendo Ácido Ferúlico. 2019. 98 f. Tese (Programa de Pós-Graduação em Química - Doutorado) - Universidade Estadual do Centro-Oeste, Guarapuava-PR. |
url |
http://tede.unicentro.br:8080/jspui/handle/jspui/1592 |
dc.language.iso.fl_str_mv |
por |
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por |
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3800526532796635565 |
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600 600 600 600 |
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1571700325303117195 |
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2075167498588264571 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual do Centro-Oeste |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Química (Doutorado) |
dc.publisher.initials.fl_str_mv |
UNICENTRO |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Unicentro::Departamento de Ciências Exatas e de Tecnologia |
publisher.none.fl_str_mv |
Universidade Estadual do Centro-Oeste |
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