GEL CONTENDO EXTRATO DE Ilex paraguariensis A. St. Hil. PARA USO CONCOMITANTE AO LED NA RECUPERAÇÃO DE LESÕES DE SEGUNDA INTENÇÃO

Detalhes bibliográficos
Autor(a) principal: Bini, Ana Carolina Dorigoni
Data de Publicação: 2021
Tipo de documento: Tese
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações do UNICENTRO
Texto Completo: http://tede.unicentro.br:8080/jspui/handle/jspui/1758
Resumo: Wound Healing is a complex and dynamic process that can be divided into phases with the goal of restoring damaged tissue and return it to homeostasis. The use of photobiomodulation can be effective in the healing of tissues, highlighting the use of LED in tissue repair. Ilex paraguariensis A. St. Hil. (Aquifoliaceae), popularly known as mate (erva-mate), is an herb specie native to southern Latin America, where it is commonly used as a tea and has great economic and social importance. In its chemical composition, there are bioactive compounds, with emphasis on compounds with antioxidant activity. The present study aimed to develop a gel for incorporating the Ilex paraguariensis extract and to verify the effects of applying the Ilex gel associated with the 627 nm LED, comparing it with the application of the gel and LED alone in the healing process in an experimental model. Thus, a 3% sodium carboxymethyl cellulose gel was developed associated with the extract of Ilex paraguariensis at 5% concentration; the chosen concentration of the polymer was ideal to achieve the desired spreadability of the product as it had the adequate viscosity. Regarding the rheological characteristics, the gel showed a non-Newtonian pseudoplastic profile. The in vivo evaluation was performed using a dorsal punch model. The sample consisted of 96 Rattus Norvegicus rats, Wistar lineage, divided into 4 groups: control group (CG) with tissue damage and application of the base gel, but without irradiation; LED group (GL) with tissue damage, application of the base gel and irradiation by LED 627 nm; LED + ILEX (GLIL) group with tissue damage, application of Ilex gel and irradiation by LED 627 nm; and ILEX group (GI) with tissue damage and application of the Ilex gel. All groups were subdivided into 3, 7, 14 and 21 days. The application method was punctual and perpendicular to the skin surface, with irradiation times of 60s, which corresponds to a dose of 7 J / cm2. Analysis of the contraction of the lesion, histological analysis, analysis of total collagen using polarized light microscopy and immunohistochemical analysis were performed. The data obtained were analyzed statistically. In the macroscopic analysis of wound contraction, it was found that LED and Ilex provided complete epidermal regeneration after 21 days of treatment, but the treatment with Ilex showed scarring remnants. The histological analysis revealed that the Ilex 9 associated with the LED and LED groups showed better healing in relation to the other groups in all the analyzed periods. An organization of the epidermis, no crust, and absence of inflammatory infiltrate were observed. In the analysis by polarized light, the groups treated with Ilex and Ilex associated with LED obtained an acceleration of the tissue regeneration process and proliferation of type I collagen in all the analyzed periods, and the group that was only treated with LED had a better proliferation of collagen at 14 and 21 days. In the immunohistochemical analysis, it was observed in all treated groups that the expression of VEGF-α and TGF-β1 were higher in the first days of treatment compared to the control groups, indicating that the healing process in these groups was accelerated. It is concluded that the CMC polymer at 3% showed superior viscosity characteristics and was more appropriate for the development of the gel containing Ilex paraguariensis for use with wound healing purposes. The 627 nm LED and the Ilex paraguariensis gel were effective in healing epidermal wounds in an animal model where both therapies have shown promise for the use intended for the healing of skin lesions, but some limitations regarding the associated use of therapies are still seen, that did not generate the optimized healing effect expected. It is suggested to adjust the concentration of the Ilex paraguariensis extract in future studies to see if its effects can become superior.
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spelling Oliveira, Paulo Renato dehttp://lattes.cnpq.br/3167101952781896036.162.959-12http://lattes.cnpq.br/0402666778625964Bini, Ana Carolina Dorigoni2021-11-22T16:10:40Z2021-05-14Bini, Ana Carolina Dorigoni. GEL CONTENDO EXTRATO DE Ilex paraguariensis A. St. Hil. PARA USO CONCOMITANTE AO LED NA RECUPERAÇÃO DE LESÕES DE SEGUNDA INTENÇÃO. 2021. 83 f. Tese (Programa de Pós-Graduação em Ciências Farmacêuticas - Doutorado) - Universidade Estadual do Centro-Oeste, Guarapuava-PR.http://tede.unicentro.br:8080/jspui/handle/jspui/1758Wound Healing is a complex and dynamic process that can be divided into phases with the goal of restoring damaged tissue and return it to homeostasis. The use of photobiomodulation can be effective in the healing of tissues, highlighting the use of LED in tissue repair. Ilex paraguariensis A. St. Hil. (Aquifoliaceae), popularly known as mate (erva-mate), is an herb specie native to southern Latin America, where it is commonly used as a tea and has great economic and social importance. In its chemical composition, there are bioactive compounds, with emphasis on compounds with antioxidant activity. The present study aimed to develop a gel for incorporating the Ilex paraguariensis extract and to verify the effects of applying the Ilex gel associated with the 627 nm LED, comparing it with the application of the gel and LED alone in the healing process in an experimental model. Thus, a 3% sodium carboxymethyl cellulose gel was developed associated with the extract of Ilex paraguariensis at 5% concentration; the chosen concentration of the polymer was ideal to achieve the desired spreadability of the product as it had the adequate viscosity. Regarding the rheological characteristics, the gel showed a non-Newtonian pseudoplastic profile. The in vivo evaluation was performed using a dorsal punch model. The sample consisted of 96 Rattus Norvegicus rats, Wistar lineage, divided into 4 groups: control group (CG) with tissue damage and application of the base gel, but without irradiation; LED group (GL) with tissue damage, application of the base gel and irradiation by LED 627 nm; LED + ILEX (GLIL) group with tissue damage, application of Ilex gel and irradiation by LED 627 nm; and ILEX group (GI) with tissue damage and application of the Ilex gel. All groups were subdivided into 3, 7, 14 and 21 days. The application method was punctual and perpendicular to the skin surface, with irradiation times of 60s, which corresponds to a dose of 7 J / cm2. Analysis of the contraction of the lesion, histological analysis, analysis of total collagen using polarized light microscopy and immunohistochemical analysis were performed. The data obtained were analyzed statistically. In the macroscopic analysis of wound contraction, it was found that LED and Ilex provided complete epidermal regeneration after 21 days of treatment, but the treatment with Ilex showed scarring remnants. The histological analysis revealed that the Ilex 9 associated with the LED and LED groups showed better healing in relation to the other groups in all the analyzed periods. An organization of the epidermis, no crust, and absence of inflammatory infiltrate were observed. In the analysis by polarized light, the groups treated with Ilex and Ilex associated with LED obtained an acceleration of the tissue regeneration process and proliferation of type I collagen in all the analyzed periods, and the group that was only treated with LED had a better proliferation of collagen at 14 and 21 days. In the immunohistochemical analysis, it was observed in all treated groups that the expression of VEGF-α and TGF-β1 were higher in the first days of treatment compared to the control groups, indicating that the healing process in these groups was accelerated. It is concluded that the CMC polymer at 3% showed superior viscosity characteristics and was more appropriate for the development of the gel containing Ilex paraguariensis for use with wound healing purposes. The 627 nm LED and the Ilex paraguariensis gel were effective in healing epidermal wounds in an animal model where both therapies have shown promise for the use intended for the healing of skin lesions, but some limitations regarding the associated use of therapies are still seen, that did not generate the optimized healing effect expected. It is suggested to adjust the concentration of the Ilex paraguariensis extract in future studies to see if its effects can become superior.A cicatrização é um processo complexo e dinâmico que pode ser dividido em fases com o objetivo de restaurar o tecido lesado e retornar a homeostase. O uso da fotobiomodulação pode ser eficaz na cicatrização de tecidos, destacando o uso do LED no reparo tecidual. Ilex paraguariensis A. St. Hil. (Aquifoliaceae), popularmente conhecida como erva-mate, é uma espécie nativa do sul da América Latina, onde é usada como bebida e tem grande importância econômica e social. Ela dispõe em sua composição química compostos bioativos, com destaque para compostos com atividade antioxidante. O presente estudo teve como objetivo desenvolver gel para incorporação do extrato de Ilex paraguariensis e verificar os efeitos da aplicação do gel Ilex associado ao LED 627 nm, comparando com a aplicação do gel e LED isoladamente no processo de cicatrização em modelo experimental. Para isso foi desenvolvido um gel de carboximetilcelulose de sódio a 3% contendo extrato de Ilex paraguariensis em concentração 5%; a concentração escolhida do polímero foi a ideal para alcançar a espalhabilidade desejada do produto pois apresentou a melhor viscosidade. Com relação as características reológicas o gel apresentou perfil pseudoplástico não-Newtoniano. A avaliação in vivo foi realizada utilizando modelo de punch dorsal. A amostra foi composta por 96 ratos da raça Rattus Norvegicus, linhagem Wistar, divididos em 4 grupos: grupo controle (GC) com lesão tecidual e aplicação do gel base, mas sem irradiação; grupo LED (GL) com lesão tecidual, aplicação do gel base e irradiação pelo LED 627 nm; grupo LED+ILEX (GLIL) com lesão tecidual, aplicação do gel Ilex e irradiação pelo LED 627 nm; e grupo ILEX (GI) com lesão tecidual e aplicação do gel Ilex. Todos os grupos foram subdivididos e avaliados com 3, 7, 14 e 21 dias. O método de aplicação foi pontual e perpendicular à superfície da pele, com tempos de irradiação de 60s, que corresponde a uma dose de 7 J/cm2. Foram realizadas análise da contração da lesão, análise histológica, análise de colágeno total utilizando microscopia de luz polarizada e análise imuno-histoquímica. Os dados obtidos foram analisados estatisticamente. Na análise macroscópica da contração da ferida constatou-se que o LED e o Ilex proporcionaram uma regeneração epidérmica completa aos 21 dias de tratamento, porém o tratamento com Ilex apresentou resquícios cicatriciais. A análise histológica revelou que os grupos Ilex associado ao LED e LED obtiveram melhor cicatrização em relação aos demais grupos em todos os períodos analisados, verificou-se uma organização da epiderme, inexistência de crosta e ausência de infiltrado inflamatório. Já na análise por luz polarizada observou-se que os grupos tratados com Ilex e Ilex associado ao LED obtiveram uma aceleração do processo de regeneração tecidual e proliferação de colágeno tipo I em todos os períodos analisados, e o tratado somente com LED teve uma melhor proliferação de colágeno aos 14 e 21 dias. Na análise imuno-histoquímica foi observado em todos os grupos tratados que a expressão de VEGF-α e TGF-β1 foram maiores nos primeiros dias de tratamento em comparação aos grupos controle, indicando que o processo de cicatrização nesses grupos foi acelerado. Conclui-se que o polímero CMC a 3% apresentou características de viscosidade superiores e mostrou-se mais apropriado para desenvolvimento do gel contendo Ilex paraguariensis para utilização com finalidade de cicatrização de feridas. O LED 627 nm e o gel de Ilex paraguariensis foram eficazes na cicatrização de feridas epidérmicas em modelo animal onde ambas as terapêuticas se mostraram promissoras para o uso destinado à cicatrização de lesões cutâneas, porém ainda são vistas algumas limitações quanto ao uso associado das terapêuticas, que não geraram o efeito otimizado de cicatrização esperado. Sendo sugerido ajustes em futuros estudos para verificar se seus efeitos podem se tornar superiores.Submitted by Fabiano Jucá (fjuca@unicentro.br) on 2021-11-22T16:10:40Z No. of bitstreams: 1 Tese ANA CAROLINA D. BINI pdf.pdf: 4035010 bytes, checksum: 449d818f9f0307be0f8b1a498fc71a3d (MD5)Made available in DSpace on 2021-11-22T16:10:40Z (GMT). No. of bitstreams: 1 Tese ANA CAROLINA D. BINI pdf.pdf: 4035010 bytes, checksum: 449d818f9f0307be0f8b1a498fc71a3d (MD5) Previous issue date: 2021-05-14Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfhttp://tede.unicentro.br:8080/jspui/retrieve/7504/Tese%20ANA%20CAROLINA%20D.%20BINI%20pdf.pdf.jpgporUniversidade Estadual do Centro-OestePrograma de Pós-Graduação em Ciências Farmacêuticas (Doutorado)UNICENTROBrasilUnicentro::Departamento de FarmáciaGelCicatrizaçãoIlex paraguariensisCarboximetilcelulose sódicaLEDGelWound HealingIlex paraguariensisSodium Carboxymethyl CelluloseLEDCIENCIAS DA SAUDE::FARMACIAGEL CONTENDO EXTRATO DE Ilex paraguariensis A. St. Hil. PARA USO CONCOMITANTE AO LED NA RECUPERAÇÃO DE LESÕES DE SEGUNDA INTENÇÃOinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis685133707775887480600600600600777590143274119112569976364134497549962075167498588264571info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações do UNICENTROinstname:Universidade Estadual do Centro-Oeste (UNICENTRO)instacron:UNICENTROTHUMBNAILTese ANA CAROLINA D. BINI pdf.pdf.jpgTese ANA CAROLINA D. BINI pdf.pdf.jpgimage/jpeg2480http://localhost:8080/tede/bitstream/jspui/1758/4/Tese+ANA+CAROLINA+D.+BINI+pdf.pdf.jpgcba9bdd31af17a993dfedfa64b57e118MD54TEXTTese ANA CAROLINA D. BINI pdf.pdf.txtTese ANA CAROLINA D. BINI pdf.pdf.txttext/plain112994http://localhost:8080/tede/bitstream/jspui/1758/3/Tese+ANA+CAROLINA+D.+BINI+pdf.pdf.txtb2c2b7e8504ab07d99793ef92120d9b8MD53ORIGINALTese ANA CAROLINA D. BINI pdf.pdfTese ANA CAROLINA D. 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dc.title.por.fl_str_mv GEL CONTENDO EXTRATO DE Ilex paraguariensis A. St. Hil. PARA USO CONCOMITANTE AO LED NA RECUPERAÇÃO DE LESÕES DE SEGUNDA INTENÇÃO
title GEL CONTENDO EXTRATO DE Ilex paraguariensis A. St. Hil. PARA USO CONCOMITANTE AO LED NA RECUPERAÇÃO DE LESÕES DE SEGUNDA INTENÇÃO
spellingShingle GEL CONTENDO EXTRATO DE Ilex paraguariensis A. St. Hil. PARA USO CONCOMITANTE AO LED NA RECUPERAÇÃO DE LESÕES DE SEGUNDA INTENÇÃO
Bini, Ana Carolina Dorigoni
Gel
Cicatrização
Ilex paraguariensis
Carboximetilcelulose sódica
LED
Gel
Wound Healing
Ilex paraguariensis
Sodium Carboxymethyl Cellulose
LED
CIENCIAS DA SAUDE::FARMACIA
title_short GEL CONTENDO EXTRATO DE Ilex paraguariensis A. St. Hil. PARA USO CONCOMITANTE AO LED NA RECUPERAÇÃO DE LESÕES DE SEGUNDA INTENÇÃO
title_full GEL CONTENDO EXTRATO DE Ilex paraguariensis A. St. Hil. PARA USO CONCOMITANTE AO LED NA RECUPERAÇÃO DE LESÕES DE SEGUNDA INTENÇÃO
title_fullStr GEL CONTENDO EXTRATO DE Ilex paraguariensis A. St. Hil. PARA USO CONCOMITANTE AO LED NA RECUPERAÇÃO DE LESÕES DE SEGUNDA INTENÇÃO
title_full_unstemmed GEL CONTENDO EXTRATO DE Ilex paraguariensis A. St. Hil. PARA USO CONCOMITANTE AO LED NA RECUPERAÇÃO DE LESÕES DE SEGUNDA INTENÇÃO
title_sort GEL CONTENDO EXTRATO DE Ilex paraguariensis A. St. Hil. PARA USO CONCOMITANTE AO LED NA RECUPERAÇÃO DE LESÕES DE SEGUNDA INTENÇÃO
author Bini, Ana Carolina Dorigoni
author_facet Bini, Ana Carolina Dorigoni
author_role author
dc.contributor.advisor1.fl_str_mv Oliveira, Paulo Renato de
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/3167101952781896
dc.contributor.authorID.fl_str_mv 036.162.959-12
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/0402666778625964
dc.contributor.author.fl_str_mv Bini, Ana Carolina Dorigoni
contributor_str_mv Oliveira, Paulo Renato de
dc.subject.por.fl_str_mv Gel
Cicatrização
Ilex paraguariensis
Carboximetilcelulose sódica
LED
topic Gel
Cicatrização
Ilex paraguariensis
Carboximetilcelulose sódica
LED
Gel
Wound Healing
Ilex paraguariensis
Sodium Carboxymethyl Cellulose
LED
CIENCIAS DA SAUDE::FARMACIA
dc.subject.eng.fl_str_mv Gel
Wound Healing
Ilex paraguariensis
Sodium Carboxymethyl Cellulose
LED
dc.subject.cnpq.fl_str_mv CIENCIAS DA SAUDE::FARMACIA
description Wound Healing is a complex and dynamic process that can be divided into phases with the goal of restoring damaged tissue and return it to homeostasis. The use of photobiomodulation can be effective in the healing of tissues, highlighting the use of LED in tissue repair. Ilex paraguariensis A. St. Hil. (Aquifoliaceae), popularly known as mate (erva-mate), is an herb specie native to southern Latin America, where it is commonly used as a tea and has great economic and social importance. In its chemical composition, there are bioactive compounds, with emphasis on compounds with antioxidant activity. The present study aimed to develop a gel for incorporating the Ilex paraguariensis extract and to verify the effects of applying the Ilex gel associated with the 627 nm LED, comparing it with the application of the gel and LED alone in the healing process in an experimental model. Thus, a 3% sodium carboxymethyl cellulose gel was developed associated with the extract of Ilex paraguariensis at 5% concentration; the chosen concentration of the polymer was ideal to achieve the desired spreadability of the product as it had the adequate viscosity. Regarding the rheological characteristics, the gel showed a non-Newtonian pseudoplastic profile. The in vivo evaluation was performed using a dorsal punch model. The sample consisted of 96 Rattus Norvegicus rats, Wistar lineage, divided into 4 groups: control group (CG) with tissue damage and application of the base gel, but without irradiation; LED group (GL) with tissue damage, application of the base gel and irradiation by LED 627 nm; LED + ILEX (GLIL) group with tissue damage, application of Ilex gel and irradiation by LED 627 nm; and ILEX group (GI) with tissue damage and application of the Ilex gel. All groups were subdivided into 3, 7, 14 and 21 days. The application method was punctual and perpendicular to the skin surface, with irradiation times of 60s, which corresponds to a dose of 7 J / cm2. Analysis of the contraction of the lesion, histological analysis, analysis of total collagen using polarized light microscopy and immunohistochemical analysis were performed. The data obtained were analyzed statistically. In the macroscopic analysis of wound contraction, it was found that LED and Ilex provided complete epidermal regeneration after 21 days of treatment, but the treatment with Ilex showed scarring remnants. The histological analysis revealed that the Ilex 9 associated with the LED and LED groups showed better healing in relation to the other groups in all the analyzed periods. An organization of the epidermis, no crust, and absence of inflammatory infiltrate were observed. In the analysis by polarized light, the groups treated with Ilex and Ilex associated with LED obtained an acceleration of the tissue regeneration process and proliferation of type I collagen in all the analyzed periods, and the group that was only treated with LED had a better proliferation of collagen at 14 and 21 days. In the immunohistochemical analysis, it was observed in all treated groups that the expression of VEGF-α and TGF-β1 were higher in the first days of treatment compared to the control groups, indicating that the healing process in these groups was accelerated. It is concluded that the CMC polymer at 3% showed superior viscosity characteristics and was more appropriate for the development of the gel containing Ilex paraguariensis for use with wound healing purposes. The 627 nm LED and the Ilex paraguariensis gel were effective in healing epidermal wounds in an animal model where both therapies have shown promise for the use intended for the healing of skin lesions, but some limitations regarding the associated use of therapies are still seen, that did not generate the optimized healing effect expected. It is suggested to adjust the concentration of the Ilex paraguariensis extract in future studies to see if its effects can become superior.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-11-22T16:10:40Z
dc.date.issued.fl_str_mv 2021-05-14
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
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dc.identifier.citation.fl_str_mv Bini, Ana Carolina Dorigoni. GEL CONTENDO EXTRATO DE Ilex paraguariensis A. St. Hil. PARA USO CONCOMITANTE AO LED NA RECUPERAÇÃO DE LESÕES DE SEGUNDA INTENÇÃO. 2021. 83 f. Tese (Programa de Pós-Graduação em Ciências Farmacêuticas - Doutorado) - Universidade Estadual do Centro-Oeste, Guarapuava-PR.
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identifier_str_mv Bini, Ana Carolina Dorigoni. GEL CONTENDO EXTRATO DE Ilex paraguariensis A. St. Hil. PARA USO CONCOMITANTE AO LED NA RECUPERAÇÃO DE LESÕES DE SEGUNDA INTENÇÃO. 2021. 83 f. Tese (Programa de Pós-Graduação em Ciências Farmacêuticas - Doutorado) - Universidade Estadual do Centro-Oeste, Guarapuava-PR.
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