Micropropagação de Amburana cearensis (Allemão) A. C. Smith
Autor(a) principal: | |
---|---|
Data de Publicação: | 2009 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UEFS |
Texto Completo: | http://tede2.uefs.br:8080/handle/tede/1255 |
Resumo: | Amburana cearensis (imburana-de-cheiro, camuru), belonging to the family Fabaceae-Papiolionoideae is a naturally occurring tree species in Caatinga. It is under threat due to over exploitation of its valuable timber and the medicinal use of its stem and seeds. The exploitation of extractive A. cearensis led this species to the list of endangered plants. In this context, the plant tissue culture is an important tool for conservation and propagation of native species, it enables the production of a large number of plants from a single individual. The objective the present work was to establish for in vitro protocol to multiplication of A. cearensis. It were evaluated the influence of different plant growth regulators (NAA, BAP and TDZ) on in vitro morphogenesis and different types of explants (leaflet, nodal segment, internodal segment, apical bud and cotyledonary segment). In the rooting stage were tested different concentrations of growth regulator IBA in the presence and absence of 1 g / L activated charcoal. The culture medium used in all experiments was the WPM supplied with 30g / L sucrose and 7g / L agar and pH adjusted to 5.7 ± 0.1 before autoclaving. The segment cotyledonary explant was considered the best for the stage of multiplication. Of the growth regulators tested, in the BAP concentration was 4.44 μM which gave the highest number of shoots (2.36). The explants leaflet and internodal segment showed no organogenic capacity. The rooted shoots better in the absence of activated charcoal. The highest number of roots was observed in medium supplemented with 10 μM of IBA. The survival rate of seedlings in acclimatization was 40%. |
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Santana, José Raniere Ferreira de5515544854999037033504http://lattes.cnpq.br/7229358410413975Campos, Vania Celene Alecrim2021-06-29T23:52:02Z2009-08-13CAMPOS, Vania Celene Alecrim. Micropropagação de Amburana cearensis (Allemão) A. C. Smith. 2009. 102 f. Dissertação (Mestrado Acadêmico em Recursos Genéticos Vegetais)- Universidade Estadual de Feira de Santana, Feira de Santana, 2009.http://tede2.uefs.br:8080/handle/tede/1255Amburana cearensis (imburana-de-cheiro, camuru), belonging to the family Fabaceae-Papiolionoideae is a naturally occurring tree species in Caatinga. It is under threat due to over exploitation of its valuable timber and the medicinal use of its stem and seeds. The exploitation of extractive A. cearensis led this species to the list of endangered plants. In this context, the plant tissue culture is an important tool for conservation and propagation of native species, it enables the production of a large number of plants from a single individual. The objective the present work was to establish for in vitro protocol to multiplication of A. cearensis. It were evaluated the influence of different plant growth regulators (NAA, BAP and TDZ) on in vitro morphogenesis and different types of explants (leaflet, nodal segment, internodal segment, apical bud and cotyledonary segment). In the rooting stage were tested different concentrations of growth regulator IBA in the presence and absence of 1 g / L activated charcoal. The culture medium used in all experiments was the WPM supplied with 30g / L sucrose and 7g / L agar and pH adjusted to 5.7 ± 0.1 before autoclaving. The segment cotyledonary explant was considered the best for the stage of multiplication. Of the growth regulators tested, in the BAP concentration was 4.44 μM which gave the highest number of shoots (2.36). The explants leaflet and internodal segment showed no organogenic capacity. The rooted shoots better in the absence of activated charcoal. The highest number of roots was observed in medium supplemented with 10 μM of IBA. The survival rate of seedlings in acclimatization was 40%.Amburana cearensis (imburana-de-cheiro, camuru), família Fabaceae–Papiolionoideae, é uma espécie arbórea que ocorre naturalmente na Caatinga. Esta espécie é bastante explorada devido às propriedades medicinais do seu caule e sementes, como também por sua utilização madeireira. A exploração extrativista de A. cearensis levou esta espécie para a lista de plantas em extinção. Neste contexto, a cultura de tecidos é uma ferramenta importante para a preservação e multiplicação de espécies nativas, pois possibilita a produção de um grande número de plantas a partir de um único indivíduo. O objetivo deste trabalho foi estabelecer metodologias para multiplicação in vitro de A. cearensis. Foram avaliados a influência de diferentes reguladores de crescimento (ANA, BAP e TDZ) sobre a morfogênese in vitro em diferentes tipos de explante (folíolo, segmento nodal, segmento internodal, gema apical e segmento cotiledonar). Na etapa do enraizamento foram testadas diferentes concentrações do regulador de crescimento AIB, na presença e ausência de 1g/L de carvão ativado. O meio de cultura utilizado em todos os experimentos foi o WPM acrescido de 30g/L sacarose e 7g/L de Ágar e o pH ajustado para 5,7±0,1 antes da autoclavagem. O segmento cotiledonar foi considerado o melhor explante para a etapa da multiplicação. Dos reguladores de crescimento testados, o BAP na concentração 4,44μM foi o que promoveu o maior número de brotos (2,36). Os explantes folíolo e segmento internodal não apresentaram capacidade organogênica. Os brotos enraizaram melhor na ausência de carvão ativado. O maior número de raiz foi observado em meio suplementado com 10 μM de AIB. A taxa de sobrevivência das mudas na aclimatização foi de 40%.Submitted by Bruno Matos Nascimento (brunomatos@uefs.br) on 2021-06-29T23:52:02Z No. of bitstreams: 1 MICROPROPAGAÇÃO de Amburana cearensis (ALLEMÃO) A. C. SMITH..pdf: 1531997 bytes, checksum: 890a47c49abb0c67479fe9a671bd922c (MD5)Made available in DSpace on 2021-06-29T23:52:02Z (GMT). No. of bitstreams: 1 MICROPROPAGAÇÃO de Amburana cearensis (ALLEMÃO) A. C. SMITH..pdf: 1531997 bytes, checksum: 890a47c49abb0c67479fe9a671bd922c (MD5) Previous issue date: 2009-08-13application/pdfporUniversidade Estadual de Feira de SantanaMestrado Acadêmico em Recursos Genéticos VegetaisUEFSBrasilDEPARTAMENTO DE CIÊNCIAS BIOLÓGICASImburana-de-cheiroCultura de tecidosOrganogêneseCitocininasEnraizamentoPlant tissue cultureOrganogenesisCytokininsRootingCIENCIAS BIOLOGICASCIENCIAS BIOLOGICAS::BOTANICAMicropropagação de Amburana cearensis (Allemão) A. C. 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dc.title.por.fl_str_mv |
Micropropagação de Amburana cearensis (Allemão) A. C. Smith |
title |
Micropropagação de Amburana cearensis (Allemão) A. C. Smith |
spellingShingle |
Micropropagação de Amburana cearensis (Allemão) A. C. Smith Campos, Vania Celene Alecrim Imburana-de-cheiro Cultura de tecidos Organogênese Citocininas Enraizamento Plant tissue culture Organogenesis Cytokinins Rooting CIENCIAS BIOLOGICAS CIENCIAS BIOLOGICAS::BOTANICA |
title_short |
Micropropagação de Amburana cearensis (Allemão) A. C. Smith |
title_full |
Micropropagação de Amburana cearensis (Allemão) A. C. Smith |
title_fullStr |
Micropropagação de Amburana cearensis (Allemão) A. C. Smith |
title_full_unstemmed |
Micropropagação de Amburana cearensis (Allemão) A. C. Smith |
title_sort |
Micropropagação de Amburana cearensis (Allemão) A. C. Smith |
author |
Campos, Vania Celene Alecrim |
author_facet |
Campos, Vania Celene Alecrim |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Santana, José Raniere Ferreira de |
dc.contributor.advisor1ID.fl_str_mv |
55155448549 |
dc.contributor.authorID.fl_str_mv |
99037033504 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/7229358410413975 |
dc.contributor.author.fl_str_mv |
Campos, Vania Celene Alecrim |
contributor_str_mv |
Santana, José Raniere Ferreira de |
dc.subject.por.fl_str_mv |
Imburana-de-cheiro Cultura de tecidos Organogênese Citocininas Enraizamento |
topic |
Imburana-de-cheiro Cultura de tecidos Organogênese Citocininas Enraizamento Plant tissue culture Organogenesis Cytokinins Rooting CIENCIAS BIOLOGICAS CIENCIAS BIOLOGICAS::BOTANICA |
dc.subject.eng.fl_str_mv |
Plant tissue culture Organogenesis Cytokinins Rooting |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS CIENCIAS BIOLOGICAS::BOTANICA |
description |
Amburana cearensis (imburana-de-cheiro, camuru), belonging to the family Fabaceae-Papiolionoideae is a naturally occurring tree species in Caatinga. It is under threat due to over exploitation of its valuable timber and the medicinal use of its stem and seeds. The exploitation of extractive A. cearensis led this species to the list of endangered plants. In this context, the plant tissue culture is an important tool for conservation and propagation of native species, it enables the production of a large number of plants from a single individual. The objective the present work was to establish for in vitro protocol to multiplication of A. cearensis. It were evaluated the influence of different plant growth regulators (NAA, BAP and TDZ) on in vitro morphogenesis and different types of explants (leaflet, nodal segment, internodal segment, apical bud and cotyledonary segment). In the rooting stage were tested different concentrations of growth regulator IBA in the presence and absence of 1 g / L activated charcoal. The culture medium used in all experiments was the WPM supplied with 30g / L sucrose and 7g / L agar and pH adjusted to 5.7 ± 0.1 before autoclaving. The segment cotyledonary explant was considered the best for the stage of multiplication. Of the growth regulators tested, in the BAP concentration was 4.44 μM which gave the highest number of shoots (2.36). The explants leaflet and internodal segment showed no organogenic capacity. The rooted shoots better in the absence of activated charcoal. The highest number of roots was observed in medium supplemented with 10 μM of IBA. The survival rate of seedlings in acclimatization was 40%. |
publishDate |
2009 |
dc.date.issued.fl_str_mv |
2009-08-13 |
dc.date.accessioned.fl_str_mv |
2021-06-29T23:52:02Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
CAMPOS, Vania Celene Alecrim. Micropropagação de Amburana cearensis (Allemão) A. C. Smith. 2009. 102 f. Dissertação (Mestrado Acadêmico em Recursos Genéticos Vegetais)- Universidade Estadual de Feira de Santana, Feira de Santana, 2009. |
dc.identifier.uri.fl_str_mv |
http://tede2.uefs.br:8080/handle/tede/1255 |
identifier_str_mv |
CAMPOS, Vania Celene Alecrim. Micropropagação de Amburana cearensis (Allemão) A. C. Smith. 2009. 102 f. Dissertação (Mestrado Acadêmico em Recursos Genéticos Vegetais)- Universidade Estadual de Feira de Santana, Feira de Santana, 2009. |
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http://tede2.uefs.br:8080/handle/tede/1255 |
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por |
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por |
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8146646481622241874 |
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openAccess |
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application/pdf |
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Universidade Estadual de Feira de Santana |
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Mestrado Acadêmico em Recursos Genéticos Vegetais |
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UEFS |
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Brasil |
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DEPARTAMENTO DE CIÊNCIAS BIOLÓGICAS |
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Universidade Estadual de Feira de Santana |
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