Caracterização em acessos de melão do Banco Ativo de Germoplasma de cucurbitáceas para o Nordeste brasileiro
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UEFS |
Texto Completo: | http://localhost:8080/tede/handle/tede/438 |
Resumo: | The melon (Cucumis melo L.) production is growing steadily in the last years and it is demanded for the internal as well as the external markets. The melon produced belongs to the botanical group inodorus, yellow type, although the cultivars used present susceptibility to biotic stresses and, therefore, there is a need to search new germplasm in order to give support to melon breeding programs. On the other hand, there are melons cultivated by small farmers in the traditional agriculture of Northeast Brazil and a sample of these melons is stored in the Active Germplasm Bank of Cucurbits for the Northeast Brazil, located in the Semiarid Embrapa, at Petrolina-PE. Thus, the present study aimed at to estimate the genetic variability within and among melon accessions using morphological and molecular markers in order to identify the melon subspecies and their respective botanical groups. In the morphological characterization the parental e S1 generations were used recording quantitative and qualitative descriptors and some accessions were partitioned in subacessios. In the molecular characterization 28 ISSR primers were used. Using the morphological data is was possible to identify the subspecies agrestis with the botanical groupsmakwua and momordica and the subespecies melo with the botanical group cantalupensis. The UPGMA clusters showed great genetic diversity within and among the accessions and subaccessions. There was a reasonable agreement between the clusters based on morphological and molecular data, although the molecular data were more precise in the separation of the clusters. However, there is indication of introgression of traits of different subspecies and their botanical groups in the melon cultivated in the traditional agriculture of Northeast Brazil. |
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Queiroz, Manoel Abilio de07415068408http://lattes.cnpq.br/7910159075791935Amorim, Clisneide Coelho de2017-02-13T21:42:15Z2016-07-19AMORIM, Clisneide Coelho de. Caracterização em acessos de melão do Banco Ativo de Germoplasma de cucurbitáceas para o Nordeste brasileiro. 2016. 63 f. Dissertação (Mestrado Acadêmico em Recursos Genéticos Vegetais)- Universidade Estadual de Feira de Santana, Feira de Santana, 2016.http://localhost:8080/tede/handle/tede/438The melon (Cucumis melo L.) production is growing steadily in the last years and it is demanded for the internal as well as the external markets. The melon produced belongs to the botanical group inodorus, yellow type, although the cultivars used present susceptibility to biotic stresses and, therefore, there is a need to search new germplasm in order to give support to melon breeding programs. On the other hand, there are melons cultivated by small farmers in the traditional agriculture of Northeast Brazil and a sample of these melons is stored in the Active Germplasm Bank of Cucurbits for the Northeast Brazil, located in the Semiarid Embrapa, at Petrolina-PE. Thus, the present study aimed at to estimate the genetic variability within and among melon accessions using morphological and molecular markers in order to identify the melon subspecies and their respective botanical groups. In the morphological characterization the parental e S1 generations were used recording quantitative and qualitative descriptors and some accessions were partitioned in subacessios. In the molecular characterization 28 ISSR primers were used. Using the morphological data is was possible to identify the subspecies agrestis with the botanical groupsmakwua and momordica and the subespecies melo with the botanical group cantalupensis. The UPGMA clusters showed great genetic diversity within and among the accessions and subaccessions. There was a reasonable agreement between the clusters based on morphological and molecular data, although the molecular data were more precise in the separation of the clusters. However, there is indication of introgression of traits of different subspecies and their botanical groups in the melon cultivated in the traditional agriculture of Northeast Brazil.A produção do melão (Cucumis melo L.) no Brasil vem aumentando significativamente nos últimos anos, sendo apreciado nos mercados internos e externos. O melão mais produzido é do grupo botânico inodorus, tipo “amarelo”, porém, as cultivares apresentam suscetibilidade a estresses bióticos e, portanto, há necessidade de busca por novo germoplasma para dar suporte aos programas de melhoramento de melão. Por outro lado, existem melões cultivados por pequenos agricultores e uma boa amostra desses melões foi coletada eencontra-se armazenada no BAG de Cucurbitáceas para o Nordeste brasileiro localizado na Embrapa Semiárido, Petrolina-PE. Assim, o presente estudo teve como objetivo estudar uma amostra de acessos de melão da agricultura tradicional e estimar a variabilidade genética entre e dentro de uma amostra de acessos desses melões utilizando descritores morfológicos e moleculares, a fim de identificar as subespécies e seus respectivos grupos botânicos. Na caracterização morfológica foram usadasas gerações parentais e S1 utilizando descritores quantitativos e qualitativos e alguns acessos foram desdobrados em subacessos. Para a caracterização molecular os subacessos foram analisados com 28 primers ISSR. Com os dados morfológicos foi possível identificar a subespécie agrestis com os grupos botânicos makuwa e momordica e a subespécie melo com o grupo botânico cantalupensis.Os agrupamentos pelo método UPGMA mostraram grande diversidade genética entre e dentro dos acessos e dos subacessos. Houve uma razoável concordância entre os dados morfológicos e moleculares, embora os dados moleculares foram mais precisos na separação dos grupos. Contudo, há indicação de que houve introgressão de caracteres das diferentes subespécies e diferentes grupos botânicos no material cultivado pelos pequenos agricultores.Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2017-02-13T21:42:15Z No. of bitstreams: 1 Dissertação Clisneide 2016.pdf: 1569914 bytes, checksum: 5fb4b229474a974cde160deeeb5bea49 (MD5)Made available in DSpace on 2017-02-13T21:42:15Z (GMT). No. of bitstreams: 1 Dissertação Clisneide 2016.pdf: 1569914 bytes, checksum: 5fb4b229474a974cde160deeeb5bea49 (MD5) Previous issue date: 2016-07-19application/pdfporUniversidade Estadual de Feira de SantanaMestrado Acadêmico em Recursos Genéticos VegetaisUEFSBrasilDEPARTAMENTO DE CIÊNCIAS BIOLÓGICASCucumis meloGermoplasmaISSRVariedade botânicaAgricultura tradicionalGermplasmBotanical varietyTraditional agricultureCIENCIAS BIOLOGICAS::BIOLOGIA GERALCaracterização em acessos de melão do Banco Ativo de Germoplasma de cucurbitáceas para o Nordeste brasileiroinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis81466464816222418746006006005026123383450589282-1634559385931244697info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UEFSinstname:Universidade Estadual de Feira de Santana (UEFS)instacron:UEFSORIGINALDissertação Clisneide 2016.pdfDissertação Clisneide 2016.pdfapplication/pdf1569914http://tede2.uefs.br:8080/bitstream/tede/438/2/Disserta%C3%A7%C3%A3o+Clisneide+2016.pdf5fb4b229474a974cde160deeeb5bea49MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82089http://tede2.uefs.br:8080/bitstream/tede/438/1/license.txt7b5ba3d2445355f386edab96125d42b7MD51tede/4382017-02-20 21:06:03.143oai:tede2.uefs.br:8080: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.uefs.br:8080/PUBhttp://tede2.uefs.br:8080/oai/requestbcuefs@uefs.br|| bcref@uefs.br||bcuefs@uefs.bropendoar:2017-02-21T00:06:03Biblioteca Digital de Teses e Dissertações da UEFS - Universidade Estadual de Feira de Santana (UEFS)false |
dc.title.por.fl_str_mv |
Caracterização em acessos de melão do Banco Ativo de Germoplasma de cucurbitáceas para o Nordeste brasileiro |
title |
Caracterização em acessos de melão do Banco Ativo de Germoplasma de cucurbitáceas para o Nordeste brasileiro |
spellingShingle |
Caracterização em acessos de melão do Banco Ativo de Germoplasma de cucurbitáceas para o Nordeste brasileiro Amorim, Clisneide Coelho de Cucumis melo Germoplasma ISSR Variedade botânica Agricultura tradicional Germplasm Botanical variety Traditional agriculture CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
title_short |
Caracterização em acessos de melão do Banco Ativo de Germoplasma de cucurbitáceas para o Nordeste brasileiro |
title_full |
Caracterização em acessos de melão do Banco Ativo de Germoplasma de cucurbitáceas para o Nordeste brasileiro |
title_fullStr |
Caracterização em acessos de melão do Banco Ativo de Germoplasma de cucurbitáceas para o Nordeste brasileiro |
title_full_unstemmed |
Caracterização em acessos de melão do Banco Ativo de Germoplasma de cucurbitáceas para o Nordeste brasileiro |
title_sort |
Caracterização em acessos de melão do Banco Ativo de Germoplasma de cucurbitáceas para o Nordeste brasileiro |
author |
Amorim, Clisneide Coelho de |
author_facet |
Amorim, Clisneide Coelho de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Queiroz, Manoel Abilio de |
dc.contributor.authorID.fl_str_mv |
07415068408 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/7910159075791935 |
dc.contributor.author.fl_str_mv |
Amorim, Clisneide Coelho de |
contributor_str_mv |
Queiroz, Manoel Abilio de |
dc.subject.por.fl_str_mv |
Cucumis melo Germoplasma ISSR Variedade botânica Agricultura tradicional |
topic |
Cucumis melo Germoplasma ISSR Variedade botânica Agricultura tradicional Germplasm Botanical variety Traditional agriculture CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
dc.subject.eng.fl_str_mv |
Germplasm Botanical variety Traditional agriculture |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
description |
The melon (Cucumis melo L.) production is growing steadily in the last years and it is demanded for the internal as well as the external markets. The melon produced belongs to the botanical group inodorus, yellow type, although the cultivars used present susceptibility to biotic stresses and, therefore, there is a need to search new germplasm in order to give support to melon breeding programs. On the other hand, there are melons cultivated by small farmers in the traditional agriculture of Northeast Brazil and a sample of these melons is stored in the Active Germplasm Bank of Cucurbits for the Northeast Brazil, located in the Semiarid Embrapa, at Petrolina-PE. Thus, the present study aimed at to estimate the genetic variability within and among melon accessions using morphological and molecular markers in order to identify the melon subspecies and their respective botanical groups. In the morphological characterization the parental e S1 generations were used recording quantitative and qualitative descriptors and some accessions were partitioned in subacessios. In the molecular characterization 28 ISSR primers were used. Using the morphological data is was possible to identify the subspecies agrestis with the botanical groupsmakwua and momordica and the subespecies melo with the botanical group cantalupensis. The UPGMA clusters showed great genetic diversity within and among the accessions and subaccessions. There was a reasonable agreement between the clusters based on morphological and molecular data, although the molecular data were more precise in the separation of the clusters. However, there is indication of introgression of traits of different subspecies and their botanical groups in the melon cultivated in the traditional agriculture of Northeast Brazil. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016-07-19 |
dc.date.accessioned.fl_str_mv |
2017-02-13T21:42:15Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
AMORIM, Clisneide Coelho de. Caracterização em acessos de melão do Banco Ativo de Germoplasma de cucurbitáceas para o Nordeste brasileiro. 2016. 63 f. Dissertação (Mestrado Acadêmico em Recursos Genéticos Vegetais)- Universidade Estadual de Feira de Santana, Feira de Santana, 2016. |
dc.identifier.uri.fl_str_mv |
http://localhost:8080/tede/handle/tede/438 |
identifier_str_mv |
AMORIM, Clisneide Coelho de. Caracterização em acessos de melão do Banco Ativo de Germoplasma de cucurbitáceas para o Nordeste brasileiro. 2016. 63 f. Dissertação (Mestrado Acadêmico em Recursos Genéticos Vegetais)- Universidade Estadual de Feira de Santana, Feira de Santana, 2016. |
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http://localhost:8080/tede/handle/tede/438 |
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por |
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por |
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8146646481622241874 |
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600 600 600 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Universidade Estadual de Feira de Santana |
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Mestrado Acadêmico em Recursos Genéticos Vegetais |
dc.publisher.initials.fl_str_mv |
UEFS |
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Brasil |
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DEPARTAMENTO DE CIÊNCIAS BIOLÓGICAS |
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Universidade Estadual de Feira de Santana |
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