Caracterização em acessos de melão do Banco Ativo de Germoplasma de cucurbitáceas para o Nordeste brasileiro

Detalhes bibliográficos
Autor(a) principal: Amorim, Clisneide Coelho de
Data de Publicação: 2016
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UEFS
Texto Completo: http://localhost:8080/tede/handle/tede/438
Resumo: The melon (Cucumis melo L.) production is growing steadily in the last years and it is demanded for the internal as well as the external markets. The melon produced belongs to the botanical group inodorus, yellow type, although the cultivars used present susceptibility to biotic stresses and, therefore, there is a need to search new germplasm in order to give support to melon breeding programs. On the other hand, there are melons cultivated by small farmers in the traditional agriculture of Northeast Brazil and a sample of these melons is stored in the Active Germplasm Bank of Cucurbits for the Northeast Brazil, located in the Semiarid Embrapa, at Petrolina-PE. Thus, the present study aimed at to estimate the genetic variability within and among melon accessions using morphological and molecular markers in order to identify the melon subspecies and their respective botanical groups. In the morphological characterization the parental e S1 generations were used recording quantitative and qualitative descriptors and some accessions were partitioned in subacessios. In the molecular characterization 28 ISSR primers were used. Using the morphological data is was possible to identify the subspecies agrestis with the botanical groupsmakwua and momordica and the subespecies melo with the botanical group cantalupensis. The UPGMA clusters showed great genetic diversity within and among the accessions and subaccessions. There was a reasonable agreement between the clusters based on morphological and molecular data, although the molecular data were more precise in the separation of the clusters. However, there is indication of introgression of traits of different subspecies and their botanical groups in the melon cultivated in the traditional agriculture of Northeast Brazil.
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spelling Queiroz, Manoel Abilio de07415068408http://lattes.cnpq.br/7910159075791935Amorim, Clisneide Coelho de2017-02-13T21:42:15Z2016-07-19AMORIM, Clisneide Coelho de. Caracterização em acessos de melão do Banco Ativo de Germoplasma de cucurbitáceas para o Nordeste brasileiro. 2016. 63 f. Dissertação (Mestrado Acadêmico em Recursos Genéticos Vegetais)- Universidade Estadual de Feira de Santana, Feira de Santana, 2016.http://localhost:8080/tede/handle/tede/438The melon (Cucumis melo L.) production is growing steadily in the last years and it is demanded for the internal as well as the external markets. The melon produced belongs to the botanical group inodorus, yellow type, although the cultivars used present susceptibility to biotic stresses and, therefore, there is a need to search new germplasm in order to give support to melon breeding programs. On the other hand, there are melons cultivated by small farmers in the traditional agriculture of Northeast Brazil and a sample of these melons is stored in the Active Germplasm Bank of Cucurbits for the Northeast Brazil, located in the Semiarid Embrapa, at Petrolina-PE. Thus, the present study aimed at to estimate the genetic variability within and among melon accessions using morphological and molecular markers in order to identify the melon subspecies and their respective botanical groups. In the morphological characterization the parental e S1 generations were used recording quantitative and qualitative descriptors and some accessions were partitioned in subacessios. In the molecular characterization 28 ISSR primers were used. Using the morphological data is was possible to identify the subspecies agrestis with the botanical groupsmakwua and momordica and the subespecies melo with the botanical group cantalupensis. The UPGMA clusters showed great genetic diversity within and among the accessions and subaccessions. There was a reasonable agreement between the clusters based on morphological and molecular data, although the molecular data were more precise in the separation of the clusters. However, there is indication of introgression of traits of different subspecies and their botanical groups in the melon cultivated in the traditional agriculture of Northeast Brazil.A produção do melão (Cucumis melo L.) no Brasil vem aumentando significativamente nos últimos anos, sendo apreciado nos mercados internos e externos. O melão mais produzido é do grupo botânico inodorus, tipo “amarelo”, porém, as cultivares apresentam suscetibilidade a estresses bióticos e, portanto, há necessidade de busca por novo germoplasma para dar suporte aos programas de melhoramento de melão. Por outro lado, existem melões cultivados por pequenos agricultores e uma boa amostra desses melões foi coletada eencontra-se armazenada no BAG de Cucurbitáceas para o Nordeste brasileiro localizado na Embrapa Semiárido, Petrolina-PE. Assim, o presente estudo teve como objetivo estudar uma amostra de acessos de melão da agricultura tradicional e estimar a variabilidade genética entre e dentro de uma amostra de acessos desses melões utilizando descritores morfológicos e moleculares, a fim de identificar as subespécies e seus respectivos grupos botânicos. Na caracterização morfológica foram usadasas gerações parentais e S1 utilizando descritores quantitativos e qualitativos e alguns acessos foram desdobrados em subacessos. Para a caracterização molecular os subacessos foram analisados com 28 primers ISSR. Com os dados morfológicos foi possível identificar a subespécie agrestis com os grupos botânicos makuwa e momordica e a subespécie melo com o grupo botânico cantalupensis.Os agrupamentos pelo método UPGMA mostraram grande diversidade genética entre e dentro dos acessos e dos subacessos. Houve uma razoável concordância entre os dados morfológicos e moleculares, embora os dados moleculares foram mais precisos na separação dos grupos. Contudo, há indicação de que houve introgressão de caracteres das diferentes subespécies e diferentes grupos botânicos no material cultivado pelos pequenos agricultores.Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2017-02-13T21:42:15Z No. of bitstreams: 1 Dissertação Clisneide 2016.pdf: 1569914 bytes, checksum: 5fb4b229474a974cde160deeeb5bea49 (MD5)Made available in DSpace on 2017-02-13T21:42:15Z (GMT). No. of bitstreams: 1 Dissertação Clisneide 2016.pdf: 1569914 bytes, checksum: 5fb4b229474a974cde160deeeb5bea49 (MD5) Previous issue date: 2016-07-19application/pdfporUniversidade Estadual de Feira de SantanaMestrado Acadêmico em Recursos Genéticos VegetaisUEFSBrasilDEPARTAMENTO DE CIÊNCIAS BIOLÓGICASCucumis meloGermoplasmaISSRVariedade botânicaAgricultura tradicionalGermplasmBotanical varietyTraditional agricultureCIENCIAS BIOLOGICAS::BIOLOGIA GERALCaracterização em acessos de melão do Banco Ativo de Germoplasma de cucurbitáceas para o Nordeste brasileiroinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis81466464816222418746006006005026123383450589282-1634559385931244697info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UEFSinstname:Universidade Estadual de Feira de Santana (UEFS)instacron:UEFSORIGINALDissertação Clisneide 2016.pdfDissertação Clisneide 2016.pdfapplication/pdf1569914http://tede2.uefs.br:8080/bitstream/tede/438/2/Disserta%C3%A7%C3%A3o+Clisneide+2016.pdf5fb4b229474a974cde160deeeb5bea49MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82089http://tede2.uefs.br:8080/bitstream/tede/438/1/license.txt7b5ba3d2445355f386edab96125d42b7MD51tede/4382017-02-20 21:06:03.143oai:tede2.uefs.br:8080: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.uefs.br:8080/PUBhttp://tede2.uefs.br:8080/oai/requestbcuefs@uefs.br|| bcref@uefs.br||bcuefs@uefs.bropendoar:2017-02-21T00:06:03Biblioteca Digital de Teses e Dissertações da UEFS - Universidade Estadual de Feira de Santana (UEFS)false
dc.title.por.fl_str_mv Caracterização em acessos de melão do Banco Ativo de Germoplasma de cucurbitáceas para o Nordeste brasileiro
title Caracterização em acessos de melão do Banco Ativo de Germoplasma de cucurbitáceas para o Nordeste brasileiro
spellingShingle Caracterização em acessos de melão do Banco Ativo de Germoplasma de cucurbitáceas para o Nordeste brasileiro
Amorim, Clisneide Coelho de
Cucumis melo
Germoplasma
ISSR
Variedade botânica
Agricultura tradicional
Germplasm
Botanical variety
Traditional agriculture
CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
title_short Caracterização em acessos de melão do Banco Ativo de Germoplasma de cucurbitáceas para o Nordeste brasileiro
title_full Caracterização em acessos de melão do Banco Ativo de Germoplasma de cucurbitáceas para o Nordeste brasileiro
title_fullStr Caracterização em acessos de melão do Banco Ativo de Germoplasma de cucurbitáceas para o Nordeste brasileiro
title_full_unstemmed Caracterização em acessos de melão do Banco Ativo de Germoplasma de cucurbitáceas para o Nordeste brasileiro
title_sort Caracterização em acessos de melão do Banco Ativo de Germoplasma de cucurbitáceas para o Nordeste brasileiro
author Amorim, Clisneide Coelho de
author_facet Amorim, Clisneide Coelho de
author_role author
dc.contributor.advisor1.fl_str_mv Queiroz, Manoel Abilio de
dc.contributor.authorID.fl_str_mv 07415068408
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/7910159075791935
dc.contributor.author.fl_str_mv Amorim, Clisneide Coelho de
contributor_str_mv Queiroz, Manoel Abilio de
dc.subject.por.fl_str_mv Cucumis melo
Germoplasma
ISSR
Variedade botânica
Agricultura tradicional
topic Cucumis melo
Germoplasma
ISSR
Variedade botânica
Agricultura tradicional
Germplasm
Botanical variety
Traditional agriculture
CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
dc.subject.eng.fl_str_mv Germplasm
Botanical variety
Traditional agriculture
dc.subject.cnpq.fl_str_mv CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
description The melon (Cucumis melo L.) production is growing steadily in the last years and it is demanded for the internal as well as the external markets. The melon produced belongs to the botanical group inodorus, yellow type, although the cultivars used present susceptibility to biotic stresses and, therefore, there is a need to search new germplasm in order to give support to melon breeding programs. On the other hand, there are melons cultivated by small farmers in the traditional agriculture of Northeast Brazil and a sample of these melons is stored in the Active Germplasm Bank of Cucurbits for the Northeast Brazil, located in the Semiarid Embrapa, at Petrolina-PE. Thus, the present study aimed at to estimate the genetic variability within and among melon accessions using morphological and molecular markers in order to identify the melon subspecies and their respective botanical groups. In the morphological characterization the parental e S1 generations were used recording quantitative and qualitative descriptors and some accessions were partitioned in subacessios. In the molecular characterization 28 ISSR primers were used. Using the morphological data is was possible to identify the subspecies agrestis with the botanical groupsmakwua and momordica and the subespecies melo with the botanical group cantalupensis. The UPGMA clusters showed great genetic diversity within and among the accessions and subaccessions. There was a reasonable agreement between the clusters based on morphological and molecular data, although the molecular data were more precise in the separation of the clusters. However, there is indication of introgression of traits of different subspecies and their botanical groups in the melon cultivated in the traditional agriculture of Northeast Brazil.
publishDate 2016
dc.date.issued.fl_str_mv 2016-07-19
dc.date.accessioned.fl_str_mv 2017-02-13T21:42:15Z
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dc.identifier.citation.fl_str_mv AMORIM, Clisneide Coelho de. Caracterização em acessos de melão do Banco Ativo de Germoplasma de cucurbitáceas para o Nordeste brasileiro. 2016. 63 f. Dissertação (Mestrado Acadêmico em Recursos Genéticos Vegetais)- Universidade Estadual de Feira de Santana, Feira de Santana, 2016.
dc.identifier.uri.fl_str_mv http://localhost:8080/tede/handle/tede/438
identifier_str_mv AMORIM, Clisneide Coelho de. Caracterização em acessos de melão do Banco Ativo de Germoplasma de cucurbitáceas para o Nordeste brasileiro. 2016. 63 f. Dissertação (Mestrado Acadêmico em Recursos Genéticos Vegetais)- Universidade Estadual de Feira de Santana, Feira de Santana, 2016.
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