Atividade antimicrobiana e composição química de méis do Estado da Bahia

Detalhes bibliográficos
Autor(a) principal: Peralta, Edna Dória
Data de Publicação: 2010
Tipo de documento: Tese
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UEFS
Texto Completo: http://tede2.uefs.br:8080/handle/tede/1188
Resumo: Antimicrobial resistance has stimulated research with honey, effective in treating various human diseases. Nevertheless, studies on its biological properties in Brazil are scarce, and specially for honeys from Bahia until now no records were found. Then, in the present work the antimicrobial activity and chemical composition of honey from Bahia was evaluated. Chapter 1 of this study evaluates 37 samples of A. mellifera honey and 16 of stingless bees (T. angustula, M. scutellaris, M. quadrifasciata and Plebeia sp), collected in Bahia, from 2005 to 2008. Chapter 2 deals with the comparison between two periods of production of A. mellifera honey from the same hive. The antimicrobial activity of honey was determined by the agar diffusion and minimal inhibitory concentration. The samples were tested against E. coli, S. aureus, P. aeruginosa and C. albicans. Parameters such as pH, acidity, water activity (aw), color and phenolic compounds were analyzed. It was realized Spearman correlation analysis to verify the relation between the physicochemical parameters and antimicrobial activity. The phenolic profile was determined by high-performance liquid chromatography and the data was treated by multivariate analysis of principal coordinates. The results showed that honey bees from Bahia have antimicrobial activity and this activity could not be attributed to just one of the analyzed parameters.
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spelling Lucchese, Angélica Maria3968463609105628962520http://lattes.cnpq.br/0955977863429457Peralta, Edna Dória2020-06-23T17:29:52Z2010-07-09PERALTA, Edna Dória. Atividade antimicrobiana e composição química de méis do Estado da Bahia. 2010. 265 f. Tese (Doutorado Acadêmico em Biotecnologia)- Universidade Estadual de Feira de Santana, Feira de Santana, 2010.http://tede2.uefs.br:8080/handle/tede/1188Antimicrobial resistance has stimulated research with honey, effective in treating various human diseases. Nevertheless, studies on its biological properties in Brazil are scarce, and specially for honeys from Bahia until now no records were found. Then, in the present work the antimicrobial activity and chemical composition of honey from Bahia was evaluated. Chapter 1 of this study evaluates 37 samples of A. mellifera honey and 16 of stingless bees (T. angustula, M. scutellaris, M. quadrifasciata and Plebeia sp), collected in Bahia, from 2005 to 2008. Chapter 2 deals with the comparison between two periods of production of A. mellifera honey from the same hive. The antimicrobial activity of honey was determined by the agar diffusion and minimal inhibitory concentration. The samples were tested against E. coli, S. aureus, P. aeruginosa and C. albicans. Parameters such as pH, acidity, water activity (aw), color and phenolic compounds were analyzed. It was realized Spearman correlation analysis to verify the relation between the physicochemical parameters and antimicrobial activity. The phenolic profile was determined by high-performance liquid chromatography and the data was treated by multivariate analysis of principal coordinates. The results showed that honey bees from Bahia have antimicrobial activity and this activity could not be attributed to just one of the analyzed parameters.A resistência microbiana tem estimulado pesquisas com o mel, eficaz no tratamento de várias afecções humanas. Apesar disso, estudos sobre suas propriedades biológicas no Brasil são escassos, e principalmente para méis da Bahia não foram encontrados registros até o momento. Assim neste trabalho a atividade antimicrobiana e a composição química de amostras de mel coletadas no estado da Bahia foram avaliadas. O Capítulo 1 deste trabalho avalia 37 amostras de méis de Apis mellifera e 16 de abelhas sem ferrão (Friseomelita doederleinei, Tetragonisca angustula, Melipona asilvai, Melipona scutellaris, Melipona quadrifasciata e Plebeia sp.), coletadas na Bahia, de 2005 a 2008. O Capítulo 2 trata da comparação entre dois períodos de produção de mel de A. mellifera da mesma colméia do município de Canarana, Bahia. A atividade antimicrobiana dos méis foi determinada pelo método de difusão em ágar e Concentração Inibitória Mínima, e a cepa de S. aureus foi a mais sensível. As amostras foram testadas contra Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa e Candida albicans. Parâmetros como pH, acidez, atividade de água (aw), cor, presença de peróxidos e compostos fenólicos foram analisados. Foi realizada análise de correlação de Spearman para verificação de possíveis relações entre os parâmetros físico-químicos e a atividade antimicrobiana. O perfil fenólico das amostras foi determinado por cromatografia líquida de alta eficiência e os dados tratados por análise multivariada de coordenadas principais. Os resultados mostram que méis de abelhas do estado da Bahia possuem atividade antimicrobiana e que esta atividade não pode ser atribuída a um único parâmetro.Submitted by Bruno Matos Nascimento (brunomatos@uefs.br) on 2020-06-23T17:29:52Z No. of bitstreams: 1 TESE_Edna Dória Peralta_2010.pdf: 6666576 bytes, checksum: e2170ca4685286bf1e2a77634a5b027e (MD5)Made available in DSpace on 2020-06-23T17:29:52Z (GMT). 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dc.title.por.fl_str_mv Atividade antimicrobiana e composição química de méis do Estado da Bahia
title Atividade antimicrobiana e composição química de méis do Estado da Bahia
spellingShingle Atividade antimicrobiana e composição química de méis do Estado da Bahia
Peralta, Edna Dória
Atividade antimicrobiana
Bahia
Mel
Antimicrobial activity
Honey
CIENCIAS BIOLOGICAS
title_short Atividade antimicrobiana e composição química de méis do Estado da Bahia
title_full Atividade antimicrobiana e composição química de méis do Estado da Bahia
title_fullStr Atividade antimicrobiana e composição química de méis do Estado da Bahia
title_full_unstemmed Atividade antimicrobiana e composição química de méis do Estado da Bahia
title_sort Atividade antimicrobiana e composição química de méis do Estado da Bahia
author Peralta, Edna Dória
author_facet Peralta, Edna Dória
author_role author
dc.contributor.advisor1.fl_str_mv Lucchese, Angélica Maria
dc.contributor.advisor1ID.fl_str_mv 39684636091
dc.contributor.authorID.fl_str_mv 05628962520
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/0955977863429457
dc.contributor.author.fl_str_mv Peralta, Edna Dória
contributor_str_mv Lucchese, Angélica Maria
dc.subject.por.fl_str_mv Atividade antimicrobiana
Bahia
Mel
topic Atividade antimicrobiana
Bahia
Mel
Antimicrobial activity
Honey
CIENCIAS BIOLOGICAS
dc.subject.eng.fl_str_mv Antimicrobial activity
Honey
dc.subject.cnpq.fl_str_mv CIENCIAS BIOLOGICAS
description Antimicrobial resistance has stimulated research with honey, effective in treating various human diseases. Nevertheless, studies on its biological properties in Brazil are scarce, and specially for honeys from Bahia until now no records were found. Then, in the present work the antimicrobial activity and chemical composition of honey from Bahia was evaluated. Chapter 1 of this study evaluates 37 samples of A. mellifera honey and 16 of stingless bees (T. angustula, M. scutellaris, M. quadrifasciata and Plebeia sp), collected in Bahia, from 2005 to 2008. Chapter 2 deals with the comparison between two periods of production of A. mellifera honey from the same hive. The antimicrobial activity of honey was determined by the agar diffusion and minimal inhibitory concentration. The samples were tested against E. coli, S. aureus, P. aeruginosa and C. albicans. Parameters such as pH, acidity, water activity (aw), color and phenolic compounds were analyzed. It was realized Spearman correlation analysis to verify the relation between the physicochemical parameters and antimicrobial activity. The phenolic profile was determined by high-performance liquid chromatography and the data was treated by multivariate analysis of principal coordinates. The results showed that honey bees from Bahia have antimicrobial activity and this activity could not be attributed to just one of the analyzed parameters.
publishDate 2010
dc.date.issued.fl_str_mv 2010-07-09
dc.date.accessioned.fl_str_mv 2020-06-23T17:29:52Z
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dc.identifier.citation.fl_str_mv PERALTA, Edna Dória. Atividade antimicrobiana e composição química de méis do Estado da Bahia. 2010. 265 f. Tese (Doutorado Acadêmico em Biotecnologia)- Universidade Estadual de Feira de Santana, Feira de Santana, 2010.
dc.identifier.uri.fl_str_mv http://tede2.uefs.br:8080/handle/tede/1188
identifier_str_mv PERALTA, Edna Dória. Atividade antimicrobiana e composição química de méis do Estado da Bahia. 2010. 265 f. Tese (Doutorado Acadêmico em Biotecnologia)- Universidade Estadual de Feira de Santana, Feira de Santana, 2010.
url http://tede2.uefs.br:8080/handle/tede/1188
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dc.publisher.department.fl_str_mv DEPARTAMENTO DE CIÊNCIAS BIOLÓGICAS
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