Detecção de espécies do vírus das estrias da bananeira (BSV) em Musa spp
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UEFS |
Texto Completo: | http://tede2.uefs.br:8080/handle/tede/896 |
Resumo: | In Brazil, the optimal climate conditionsenable banana production in the entire country. However, some phytopatogens such as fungi, nematodes, insects and vírus compromise yield and fruit quality. Among the vírus diseases, banana streak vírus (BSV) is the most important, whereas more than fifteen different species have been identified worldwide. The objectives of the present study were to identify BSV species in germplasm bank of Embrapa and in different banana production regions in Brazil by molecular characterization and indexation using IC-PCR(Immunocapture-PCR) + DNase, as well as map the presence of infective fragments in commercial germplasm and those of genome BB used in the crosses. Leaves of 300 banana accessions from the Embrapa ex situ germplasm collection and 39in vitro samples, were collected. Two-hundred and twenty-seven samples from eight different banana production regions in Brazil, were also collected. Twenty-three species-specific primers for detection of endogenous BSV (eBSV) for species Goldfinger(eBSGFV), Imovè (eBSIMV) and Obinol'Ewai (eBSOLV) were also used for the analysis of incidence and prevalence of viral species. The analysis of the 300 accessions of BAG showed that most samples were negative for eBSV and of the positive banana samples for eBSV, 20 were infected by episomal BSV of the BSOLV and BSGFV species. None of the analyzed samples contained BSV episomal of the BSIMV species. Most of the samples analyzed from the eight different banana producing regions in Brazil was positive for the presence of at least one of the eBSV species and only samples from the State of São Paulo and one from Santa Catarina presented episomal BSV. The study with hybrids developed by Embrapa identified presence of the infective fragment GF7 associated to the BSGFV species. Regarding the presence of the infective fragment associated to the BSOLV species, only the Thap Maeo cultivar was positive. The other genotypes amplified only the non-infective fragment, OL2. All eight accessions with BB genome presented either the infective fragment for the BSOLV species as well as for the BSGFV species. The IC-PCR + DNAse indexation confirmed the low presence of episomal BSV in the analyzed samples. |
id |
UEFS_d1713439d047b646d855c33552bf3876 |
---|---|
oai_identifier_str |
oai:tede2.uefs.br:8080:tede/896 |
network_acronym_str |
UEFS |
network_name_str |
Biblioteca Digital de Teses e Dissertações da UEFS |
repository_id_str |
|
spelling |
Amorim, Edson Perito90982452004Ferreira, Cláudia FortesAmorim, Vanusia B. de Oliveira03318333522http://lattes.cnpq.br/6666505911630893Caldas, Camila Nogueira Pestana2019-10-04T18:29:52Z2019-06-27CALDAS, Camila Nogueira Pestana. Detecção de espécies do vírus das estrias da bananeira (BSV) em Musa spp. 2019. 99 f. Tese (Doutorado Acadêmico em Biotecnologia)- Universidade Estadual de Feira de Santana, Feira de Santana, 2019.http://tede2.uefs.br:8080/handle/tede/896In Brazil, the optimal climate conditionsenable banana production in the entire country. However, some phytopatogens such as fungi, nematodes, insects and vírus compromise yield and fruit quality. Among the vírus diseases, banana streak vírus (BSV) is the most important, whereas more than fifteen different species have been identified worldwide. The objectives of the present study were to identify BSV species in germplasm bank of Embrapa and in different banana production regions in Brazil by molecular characterization and indexation using IC-PCR(Immunocapture-PCR) + DNase, as well as map the presence of infective fragments in commercial germplasm and those of genome BB used in the crosses. Leaves of 300 banana accessions from the Embrapa ex situ germplasm collection and 39in vitro samples, were collected. Two-hundred and twenty-seven samples from eight different banana production regions in Brazil, were also collected. Twenty-three species-specific primers for detection of endogenous BSV (eBSV) for species Goldfinger(eBSGFV), Imovè (eBSIMV) and Obinol'Ewai (eBSOLV) were also used for the analysis of incidence and prevalence of viral species. The analysis of the 300 accessions of BAG showed that most samples were negative for eBSV and of the positive banana samples for eBSV, 20 were infected by episomal BSV of the BSOLV and BSGFV species. None of the analyzed samples contained BSV episomal of the BSIMV species. Most of the samples analyzed from the eight different banana producing regions in Brazil was positive for the presence of at least one of the eBSV species and only samples from the State of São Paulo and one from Santa Catarina presented episomal BSV. The study with hybrids developed by Embrapa identified presence of the infective fragment GF7 associated to the BSGFV species. Regarding the presence of the infective fragment associated to the BSOLV species, only the Thap Maeo cultivar was positive. The other genotypes amplified only the non-infective fragment, OL2. All eight accessions with BB genome presented either the infective fragment for the BSOLV species as well as for the BSGFV species. The IC-PCR + DNAse indexation confirmed the low presence of episomal BSV in the analyzed samples.No Brasil, o clima favorável possibilita a produção de banana em todo território. No entanto, alguns fitopatógenos prejudicam a produção da banana, tais como fungos, nematóides, insetos e vírus, comprometendo a produtividade e a qualidade final dos frutos. Entre as viroses mais comuns, destaca-se o vírus das estrias da bananeira (BSV), que possui mais de quinze diferentes espécies identificadas em todo o mundo. O presente estudo teve como objetivos realizar um levantamento das espécies de BSV presentes no Banco Ativo de Germoplasma de Banana da Embrapa e em diferentes regiões produtoras de banana do Brasil, por meio da caracterização molecular e indexação a partir do uso da técnica IC-PCR (Immunocapture-PCR) +DNase, bem como mapear a presença de fragmentos infectivos no germoplasma comercial e diploides BB com potencial de uso em cruzamentos. Para tanto, foram coletadas amostras de folhas de 300 acessos de bananeira da coleção de germoplasma da Embrapa provenientes do cultivo ex situ e 39 amostras do cultivo in vitro. Também foram coletadas 237 amostras provenientes de oito diferentes regiões produtoras de banana do Brasil. Para a análise de incidência e prevalência de espécies do vírus, foram utilizados 23 iniciadores espécie-específicos para detectar a presença do vírus das estrias da bananeira endógeno (eBSV), a saber Goldfinger (eBSGFV), Imovè (eBSIMV) e Obinol'Ewai (eBSOLV). A análise dos 300 acessos do BAG permitiu observar que a maioria apresentou resultados negativos quanto à presença do eBSV. Das amostras de bananeira positivas, 20 apresentaram infecção por BSV epissomal das espécies BSOLV e BSGFV. Nenhuma amostra apresentou o BSV epissomal da espécie BSIMV.Dentre os acessos do cultivo in vitro indexados não foi constatado a presença do BSV epissomal das espécies BSGFV e BSIMV. Também se observou que a maioria das amostras analisadas das oito diferentes regiões produtoras do Brasil foi positiva para a presença de ao menos uma espécie de eBSV e apenas as amostras dos Estados de São Paulo e outra de Santa Catarina apresentaram a presença do BSV epissomal. O estudo com os híbridos desenvolvidos pela Embrapa identificou a presença do fragmento infectivo GF7 associado à espécie BSGFV. Em se tratando da presença do fragmento infectivo associado à espécie BSOLV, apenas a cultivar Thap Maeo mostrou-se positiva. Os outros genótipos amplificaram apenas o fragmento não infectivo OL2. Observou-se que todos os oito acessos de genoma BB apresentam tanto o fragmento viral infeccioso para a espécie BSOLV quanto o fragmento infeccioso para a espécie viral BSGFV. A indexação por IC-PCR + DNAse confirmou à baixa presença do BSV epissomal observado nas amostras analisadas.Submitted by Carolina Neves (carolinapon@uefs.br) on 2019-10-04T18:29:52Z No. of bitstreams: 1 Tese camila.pdf: 1895389 bytes, checksum: 794cd07dd904635c5cf8b1f31c174bb0 (MD5)Made available in DSpace on 2019-10-04T18:29:52Z (GMT). No. of bitstreams: 1 Tese camila.pdf: 1895389 bytes, checksum: 794cd07dd904635c5cf8b1f31c174bb0 (MD5) Previous issue date: 2019-06-27Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfporUniversidade Estadual de Feira de SantanaDoutorado Acadêmico em BiotecnologiaUEFSBrasilDEPARTAMENTO DE CIÊNCIAS BIOLÓGICASBananeiraIndexaçãoBanana Streak Goldfinger VírusBanana Streak Obinol’Ewai VírusBanana Streak Imovè VírusBanana plantIndexationCIENCIAS BIOLOGICAS::BOTANICABOTANICA::BOTANICA APLICADADetecção de espécies do vírus das estrias da bananeira (BSV) em Musa sppinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis-68152692297897915436006006006006005026123383450589282-340614789241430750159250118293197842713590462550136975366info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UEFSinstname:Universidade Estadual de Feira de Santana (UEFS)instacron:UEFSORIGINALTese camila.pdfTese camila.pdfapplication/pdf1895389http://tede2.uefs.br:8080/bitstream/tede/896/2/Tese+camila.pdf794cd07dd904635c5cf8b1f31c174bb0MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82089http://tede2.uefs.br:8080/bitstream/tede/896/1/license.txt7b5ba3d2445355f386edab96125d42b7MD51tede/8962019-10-04 15:29:52.479oai:tede2.uefs.br:8080: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.uefs.br:8080/PUBhttp://tede2.uefs.br:8080/oai/requestbcuefs@uefs.br|| bcref@uefs.br||bcuefs@uefs.bropendoar:2019-10-04T18:29:52Biblioteca Digital de Teses e Dissertações da UEFS - Universidade Estadual de Feira de Santana (UEFS)false |
dc.title.por.fl_str_mv |
Detecção de espécies do vírus das estrias da bananeira (BSV) em Musa spp |
title |
Detecção de espécies do vírus das estrias da bananeira (BSV) em Musa spp |
spellingShingle |
Detecção de espécies do vírus das estrias da bananeira (BSV) em Musa spp Caldas, Camila Nogueira Pestana Bananeira Indexação Banana Streak Goldfinger Vírus Banana Streak Obinol’Ewai Vírus Banana Streak Imovè Vírus Banana plant Indexation CIENCIAS BIOLOGICAS::BOTANICA BOTANICA::BOTANICA APLICADA |
title_short |
Detecção de espécies do vírus das estrias da bananeira (BSV) em Musa spp |
title_full |
Detecção de espécies do vírus das estrias da bananeira (BSV) em Musa spp |
title_fullStr |
Detecção de espécies do vírus das estrias da bananeira (BSV) em Musa spp |
title_full_unstemmed |
Detecção de espécies do vírus das estrias da bananeira (BSV) em Musa spp |
title_sort |
Detecção de espécies do vírus das estrias da bananeira (BSV) em Musa spp |
author |
Caldas, Camila Nogueira Pestana |
author_facet |
Caldas, Camila Nogueira Pestana |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Amorim, Edson Perito |
dc.contributor.advisor1ID.fl_str_mv |
90982452004 |
dc.contributor.advisor-co1.fl_str_mv |
Ferreira, Cláudia Fortes |
dc.contributor.advisor-co2.fl_str_mv |
Amorim, Vanusia B. de Oliveira |
dc.contributor.authorID.fl_str_mv |
03318333522 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/6666505911630893 |
dc.contributor.author.fl_str_mv |
Caldas, Camila Nogueira Pestana |
contributor_str_mv |
Amorim, Edson Perito Ferreira, Cláudia Fortes Amorim, Vanusia B. de Oliveira |
dc.subject.por.fl_str_mv |
Bananeira Indexação Banana Streak Goldfinger Vírus Banana Streak Obinol’Ewai Vírus Banana Streak Imovè Vírus |
topic |
Bananeira Indexação Banana Streak Goldfinger Vírus Banana Streak Obinol’Ewai Vírus Banana Streak Imovè Vírus Banana plant Indexation CIENCIAS BIOLOGICAS::BOTANICA BOTANICA::BOTANICA APLICADA |
dc.subject.eng.fl_str_mv |
Banana plant Indexation |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::BOTANICA BOTANICA::BOTANICA APLICADA |
description |
In Brazil, the optimal climate conditionsenable banana production in the entire country. However, some phytopatogens such as fungi, nematodes, insects and vírus compromise yield and fruit quality. Among the vírus diseases, banana streak vírus (BSV) is the most important, whereas more than fifteen different species have been identified worldwide. The objectives of the present study were to identify BSV species in germplasm bank of Embrapa and in different banana production regions in Brazil by molecular characterization and indexation using IC-PCR(Immunocapture-PCR) + DNase, as well as map the presence of infective fragments in commercial germplasm and those of genome BB used in the crosses. Leaves of 300 banana accessions from the Embrapa ex situ germplasm collection and 39in vitro samples, were collected. Two-hundred and twenty-seven samples from eight different banana production regions in Brazil, were also collected. Twenty-three species-specific primers for detection of endogenous BSV (eBSV) for species Goldfinger(eBSGFV), Imovè (eBSIMV) and Obinol'Ewai (eBSOLV) were also used for the analysis of incidence and prevalence of viral species. The analysis of the 300 accessions of BAG showed that most samples were negative for eBSV and of the positive banana samples for eBSV, 20 were infected by episomal BSV of the BSOLV and BSGFV species. None of the analyzed samples contained BSV episomal of the BSIMV species. Most of the samples analyzed from the eight different banana producing regions in Brazil was positive for the presence of at least one of the eBSV species and only samples from the State of São Paulo and one from Santa Catarina presented episomal BSV. The study with hybrids developed by Embrapa identified presence of the infective fragment GF7 associated to the BSGFV species. Regarding the presence of the infective fragment associated to the BSOLV species, only the Thap Maeo cultivar was positive. The other genotypes amplified only the non-infective fragment, OL2. All eight accessions with BB genome presented either the infective fragment for the BSOLV species as well as for the BSGFV species. The IC-PCR + DNAse indexation confirmed the low presence of episomal BSV in the analyzed samples. |
publishDate |
2019 |
dc.date.accessioned.fl_str_mv |
2019-10-04T18:29:52Z |
dc.date.issued.fl_str_mv |
2019-06-27 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
CALDAS, Camila Nogueira Pestana. Detecção de espécies do vírus das estrias da bananeira (BSV) em Musa spp. 2019. 99 f. Tese (Doutorado Acadêmico em Biotecnologia)- Universidade Estadual de Feira de Santana, Feira de Santana, 2019. |
dc.identifier.uri.fl_str_mv |
http://tede2.uefs.br:8080/handle/tede/896 |
identifier_str_mv |
CALDAS, Camila Nogueira Pestana. Detecção de espécies do vírus das estrias da bananeira (BSV) em Musa spp. 2019. 99 f. Tese (Doutorado Acadêmico em Biotecnologia)- Universidade Estadual de Feira de Santana, Feira de Santana, 2019. |
url |
http://tede2.uefs.br:8080/handle/tede/896 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
-6815269229789791543 |
dc.relation.confidence.fl_str_mv |
600 600 600 600 600 |
dc.relation.department.fl_str_mv |
5026123383450589282 |
dc.relation.cnpq.fl_str_mv |
-3406147892414307501 5925011829319784271 |
dc.relation.sponsorship.fl_str_mv |
3590462550136975366 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Feira de Santana |
dc.publisher.program.fl_str_mv |
Doutorado Acadêmico em Biotecnologia |
dc.publisher.initials.fl_str_mv |
UEFS |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
DEPARTAMENTO DE CIÊNCIAS BIOLÓGICAS |
publisher.none.fl_str_mv |
Universidade Estadual de Feira de Santana |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da UEFS instname:Universidade Estadual de Feira de Santana (UEFS) instacron:UEFS |
instname_str |
Universidade Estadual de Feira de Santana (UEFS) |
instacron_str |
UEFS |
institution |
UEFS |
reponame_str |
Biblioteca Digital de Teses e Dissertações da UEFS |
collection |
Biblioteca Digital de Teses e Dissertações da UEFS |
bitstream.url.fl_str_mv |
http://tede2.uefs.br:8080/bitstream/tede/896/2/Tese+camila.pdf http://tede2.uefs.br:8080/bitstream/tede/896/1/license.txt |
bitstream.checksum.fl_str_mv |
794cd07dd904635c5cf8b1f31c174bb0 7b5ba3d2445355f386edab96125d42b7 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da UEFS - Universidade Estadual de Feira de Santana (UEFS) |
repository.mail.fl_str_mv |
bcuefs@uefs.br|| bcref@uefs.br||bcuefs@uefs.br |
_version_ |
1809288778284531712 |