Estudo dos efeitos fisiopatológicos locais e renais induzidos pela peçonha de Crotalus durissus ssp. do estado da Bahia
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UEFS |
Texto Completo: | http://tede2.uefs.br:8080/handle/tede/798 |
Resumo: | The ophidian accidents involving species of the genus Crotalus are considered serious and present a high number of deaths. The effects triggered after accidents with this genus can vary intra and interspecifically due to the wide variability of the composition of the venom. Thus, this study evaluated the possible local and renal damages triggered by the venom of Crotalus durissus ssp. of the State of Bahia. For this, male Swiss mice weighing between 20 and 22 g separated in control (inoculated with PBS) and venom (inoculated with 1.5 μg of the Cd-ssp venom) were used. Local damage was assessed by quantification of the local edematogenic reaction, of the cytokines (IL-6 and IL-1β) and by leukocyte migration. Myotoxic damage was assessed by the analysis of creatine kinase (CK) levels. Renal toxicity was assessed, following venom exposure times of 6, 12, 24 and 72 hours, from the analysis of serum creatinine and urea levels, of the sodium excretion fractions (% FENa) and potassium (% FEK), of the levels of malondialdehyde (MDA) and myeloperoxidase (MPO) and of the inflammatory markers (IL-1β, CXCL-1 and IL-18). In addition, the histological analysis of the interplantar region of the plantar cushion, the gastrocnemius musculature and the renal tissue was made. It was observed that the Cd-ssp venom triggered: a) local inflammatory process, assessed by the production of marked edema, Il-1β, Il-6, and the recruitment of leukocytes to the site of inoculation; b) myotoxic process, indicated by increased CK levels at 6, 12 and 24h after inoculation; c) nephrotoxic process confirmed by increased serum creatinine levels (24 and 72 hours), by the increase of % FENa (24 e 72h), of % FEK (72h), by the increase of MPO and of the relative expression of the KIM-1 (12h), Il-1β (12h and 24h) and CxCL-1 (12h); d) increased renal tissue oxidative process by increasing MDA levels. The histological technique demonstrated the presence of inflammatory infiltrate and edema in the interplantar region, necrosis and inflammatory infiltrate in the plantar cushion musculature and gastrocnemius and interstitial vascular ectasia, disorganization of the tubular architecture with marked vacuolization and degeneration of the renal tubular epithelium. It was concluded, therefore, that Cd-ssp venom promoted local alterations, unlike other Brazilian Crotalus, and caused renal alterations confirmed by biochemical, histological and molecular parameters. |
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Biondi, Ilka04089705533http://lattes.cnpq.br/7008141550961609Cabral, Ívila Santos2019-07-17T20:42:58Z2018-09-21CABRAL, Ívila Santos. Estudo dos efeitos fisiopatológicos locais e renais induzidos pela peçonha de Crotalus durissus ssp. do estado da Bahia. 2018. 98 f. Dissertação (Mestrado Acadêmico em Ciências Farmacêuticas)- Universidade Estadual de Feira de Santana, Feira de Santana, 2018.http://tede2.uefs.br:8080/handle/tede/798The ophidian accidents involving species of the genus Crotalus are considered serious and present a high number of deaths. The effects triggered after accidents with this genus can vary intra and interspecifically due to the wide variability of the composition of the venom. Thus, this study evaluated the possible local and renal damages triggered by the venom of Crotalus durissus ssp. of the State of Bahia. For this, male Swiss mice weighing between 20 and 22 g separated in control (inoculated with PBS) and venom (inoculated with 1.5 μg of the Cd-ssp venom) were used. Local damage was assessed by quantification of the local edematogenic reaction, of the cytokines (IL-6 and IL-1β) and by leukocyte migration. Myotoxic damage was assessed by the analysis of creatine kinase (CK) levels. Renal toxicity was assessed, following venom exposure times of 6, 12, 24 and 72 hours, from the analysis of serum creatinine and urea levels, of the sodium excretion fractions (% FENa) and potassium (% FEK), of the levels of malondialdehyde (MDA) and myeloperoxidase (MPO) and of the inflammatory markers (IL-1β, CXCL-1 and IL-18). In addition, the histological analysis of the interplantar region of the plantar cushion, the gastrocnemius musculature and the renal tissue was made. It was observed that the Cd-ssp venom triggered: a) local inflammatory process, assessed by the production of marked edema, Il-1β, Il-6, and the recruitment of leukocytes to the site of inoculation; b) myotoxic process, indicated by increased CK levels at 6, 12 and 24h after inoculation; c) nephrotoxic process confirmed by increased serum creatinine levels (24 and 72 hours), by the increase of % FENa (24 e 72h), of % FEK (72h), by the increase of MPO and of the relative expression of the KIM-1 (12h), Il-1β (12h and 24h) and CxCL-1 (12h); d) increased renal tissue oxidative process by increasing MDA levels. The histological technique demonstrated the presence of inflammatory infiltrate and edema in the interplantar region, necrosis and inflammatory infiltrate in the plantar cushion musculature and gastrocnemius and interstitial vascular ectasia, disorganization of the tubular architecture with marked vacuolization and degeneration of the renal tubular epithelium. It was concluded, therefore, that Cd-ssp venom promoted local alterations, unlike other Brazilian Crotalus, and caused renal alterations confirmed by biochemical, histological and molecular parameters.Os acidentes ofídicos envolvendo as espécies do gênero Crotalus são considerados graves e apresentam número elevado de óbitos. Os efeitos desencadeados após acidentes com este gênero podem variar de forma intra e interespecífica em decorrência da ampla variabilidade da composição da peçonha. Dessa forma, este trabalho avaliou os possíveis danos locais e renais desencadeados pela peçonha de Crotalus durissus ssp. do Estado da Bahia. Para tanto, foram utilizados camundongos Swiss machos, pesando entre 20 e 22g separados em grupos controle (inoculados com PBS) e peçonha (inoculados com 1,5ug da peçonha de Cd-ssp). Os danos locais foram avaliados pela quantificação da reação edematogênica local, de citocinas (IL-6 e IL-1β) e pela migração leucocitária. Danos miotóxicos foram avaliados pela análise dos níveis de Creatina quinase (CK). A toxicidade renal foi avaliada, seguindo tempos de exposição à peçonha de 6, 12, 24 e 72horas, a partir da análise dos níveis séricos de creatinina e ureia, das frações de excreção de sódio (%FENa) e de potássio (%FEK), dos níveis de malondialdeído (MDA) e de mieloperoxidade (MPO) e, da expressão gênica do biomarcador precoce de lesão renal (KIM-1) e dos marcadores inflamatórios (IL-1β, CXCL-1 e IL-18). Além disso, foi feita a análise histológica da região interplantar do coxim plantar, da musculatura do gastrocnêmio e do tecido renal. Foi observado que a peçonha de Cd-ssp desencadeou: a) processo inflamatório local, avaliado pela produção de edema acentuado, de Il-1β, de Il-6 e pelo recrutamento de leucócitos para o local da inoculação; b) processo miotóxico, indicado pelo aumento dos níveis de CK em 6, 12 e 24h após a inoculação; c) processo nefrotóxico confirmado pelo aumento dos níveis séricos de creatinina (24 e 72h), pelo aumento da %FENa (24 e 72h), da %FEK (72h), pelo aumento de MPO e da expressão relativa do KIM-1 (12h), de Il-1β (12h e 24h) e de CxCL-1(12h); d) aumento do processo oxidativo do tecido renal pelo aumento dos níveis de MDA. A técnica histológica demonstrou a presença de infiltrado inflamatório e edema na região interplantar, necrose e infiltrado inflamatório na musculatura do coxim plantar e do gastrocnêmio e ectasia vascular intersticial, desorganização da arquitetura tubular com marcante vacuolização e degeneração do epitélio tubular renal. Conclui-se, assim, que a peçonha de Cd-ssp promoveu alterações locais, diferentemente de outras Crotalus brasileiras e causou alterações renais comprovadas por parâmetros bioquímicos, histológicos e moleculares.Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2019-07-17T20:42:58Z No. of bitstreams: 1 Dissertação final Ivila Santos Cabral.pdf: 2808985 bytes, checksum: 99b9097c52c73d23a77af50edd83e8f5 (MD5)Made available in DSpace on 2019-07-17T20:42:58Z (GMT). No. of bitstreams: 1 Dissertação final Ivila Santos Cabral.pdf: 2808985 bytes, checksum: 99b9097c52c73d23a77af50edd83e8f5 (MD5) Previous issue date: 2018-09-21Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfporUniversidade Estadual de Feira de SantanaMestrado Acadêmico em Ciências FarmacêuticasUEFSBrasilDEPARTAMENTO DE SAÚDECrotalus durissus ssp.Edema localLesão renal agudaBiomarcadores renaisLocal edemaAcute kidney injuryRenal biomarkersCIENCIAS BIOLOGICAS::IMUNOLOGIAEstudo dos efeitos fisiopatológicos locais e renais induzidos pela peçonha de Crotalus durissus ssp. do estado da Bahiainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-5875843737201070632600600600600100639556947147789059899191883767476143590462550136975366info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UEFSinstname:Universidade Estadual de Feira de Santana (UEFS)instacron:UEFSORIGINALDissertação final Ivila Santos Cabral.pdfDissertação final Ivila Santos Cabral.pdfapplication/pdf2808985http://tede2.uefs.br:8080/bitstream/tede/798/2/Disserta%C3%A7%C3%A3o+final+Ivila+Santos+Cabral.pdf99b9097c52c73d23a77af50edd83e8f5MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82089http://tede2.uefs.br:8080/bitstream/tede/798/1/license.txt7b5ba3d2445355f386edab96125d42b7MD51tede/7982019-07-17 17:42:58.575oai:tede2.uefs.br:8080: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.uefs.br:8080/PUBhttp://tede2.uefs.br:8080/oai/requestbcuefs@uefs.br|| bcref@uefs.br||bcuefs@uefs.bropendoar:2019-07-17T20:42:58Biblioteca Digital de Teses e Dissertações da UEFS - Universidade Estadual de Feira de Santana (UEFS)false |
dc.title.por.fl_str_mv |
Estudo dos efeitos fisiopatológicos locais e renais induzidos pela peçonha de Crotalus durissus ssp. do estado da Bahia |
title |
Estudo dos efeitos fisiopatológicos locais e renais induzidos pela peçonha de Crotalus durissus ssp. do estado da Bahia |
spellingShingle |
Estudo dos efeitos fisiopatológicos locais e renais induzidos pela peçonha de Crotalus durissus ssp. do estado da Bahia Cabral, Ívila Santos Crotalus durissus ssp. Edema local Lesão renal aguda Biomarcadores renais Local edema Acute kidney injury Renal biomarkers CIENCIAS BIOLOGICAS::IMUNOLOGIA |
title_short |
Estudo dos efeitos fisiopatológicos locais e renais induzidos pela peçonha de Crotalus durissus ssp. do estado da Bahia |
title_full |
Estudo dos efeitos fisiopatológicos locais e renais induzidos pela peçonha de Crotalus durissus ssp. do estado da Bahia |
title_fullStr |
Estudo dos efeitos fisiopatológicos locais e renais induzidos pela peçonha de Crotalus durissus ssp. do estado da Bahia |
title_full_unstemmed |
Estudo dos efeitos fisiopatológicos locais e renais induzidos pela peçonha de Crotalus durissus ssp. do estado da Bahia |
title_sort |
Estudo dos efeitos fisiopatológicos locais e renais induzidos pela peçonha de Crotalus durissus ssp. do estado da Bahia |
author |
Cabral, Ívila Santos |
author_facet |
Cabral, Ívila Santos |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Biondi, Ilka |
dc.contributor.authorID.fl_str_mv |
04089705533 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/7008141550961609 |
dc.contributor.author.fl_str_mv |
Cabral, Ívila Santos |
contributor_str_mv |
Biondi, Ilka |
dc.subject.por.fl_str_mv |
Crotalus durissus ssp. Edema local Lesão renal aguda Biomarcadores renais |
topic |
Crotalus durissus ssp. Edema local Lesão renal aguda Biomarcadores renais Local edema Acute kidney injury Renal biomarkers CIENCIAS BIOLOGICAS::IMUNOLOGIA |
dc.subject.eng.fl_str_mv |
Local edema Acute kidney injury Renal biomarkers |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::IMUNOLOGIA |
description |
The ophidian accidents involving species of the genus Crotalus are considered serious and present a high number of deaths. The effects triggered after accidents with this genus can vary intra and interspecifically due to the wide variability of the composition of the venom. Thus, this study evaluated the possible local and renal damages triggered by the venom of Crotalus durissus ssp. of the State of Bahia. For this, male Swiss mice weighing between 20 and 22 g separated in control (inoculated with PBS) and venom (inoculated with 1.5 μg of the Cd-ssp venom) were used. Local damage was assessed by quantification of the local edematogenic reaction, of the cytokines (IL-6 and IL-1β) and by leukocyte migration. Myotoxic damage was assessed by the analysis of creatine kinase (CK) levels. Renal toxicity was assessed, following venom exposure times of 6, 12, 24 and 72 hours, from the analysis of serum creatinine and urea levels, of the sodium excretion fractions (% FENa) and potassium (% FEK), of the levels of malondialdehyde (MDA) and myeloperoxidase (MPO) and of the inflammatory markers (IL-1β, CXCL-1 and IL-18). In addition, the histological analysis of the interplantar region of the plantar cushion, the gastrocnemius musculature and the renal tissue was made. It was observed that the Cd-ssp venom triggered: a) local inflammatory process, assessed by the production of marked edema, Il-1β, Il-6, and the recruitment of leukocytes to the site of inoculation; b) myotoxic process, indicated by increased CK levels at 6, 12 and 24h after inoculation; c) nephrotoxic process confirmed by increased serum creatinine levels (24 and 72 hours), by the increase of % FENa (24 e 72h), of % FEK (72h), by the increase of MPO and of the relative expression of the KIM-1 (12h), Il-1β (12h and 24h) and CxCL-1 (12h); d) increased renal tissue oxidative process by increasing MDA levels. The histological technique demonstrated the presence of inflammatory infiltrate and edema in the interplantar region, necrosis and inflammatory infiltrate in the plantar cushion musculature and gastrocnemius and interstitial vascular ectasia, disorganization of the tubular architecture with marked vacuolization and degeneration of the renal tubular epithelium. It was concluded, therefore, that Cd-ssp venom promoted local alterations, unlike other Brazilian Crotalus, and caused renal alterations confirmed by biochemical, histological and molecular parameters. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018-09-21 |
dc.date.accessioned.fl_str_mv |
2019-07-17T20:42:58Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
CABRAL, Ívila Santos. Estudo dos efeitos fisiopatológicos locais e renais induzidos pela peçonha de Crotalus durissus ssp. do estado da Bahia. 2018. 98 f. Dissertação (Mestrado Acadêmico em Ciências Farmacêuticas)- Universidade Estadual de Feira de Santana, Feira de Santana, 2018. |
dc.identifier.uri.fl_str_mv |
http://tede2.uefs.br:8080/handle/tede/798 |
identifier_str_mv |
CABRAL, Ívila Santos. Estudo dos efeitos fisiopatológicos locais e renais induzidos pela peçonha de Crotalus durissus ssp. do estado da Bahia. 2018. 98 f. Dissertação (Mestrado Acadêmico em Ciências Farmacêuticas)- Universidade Estadual de Feira de Santana, Feira de Santana, 2018. |
url |
http://tede2.uefs.br:8080/handle/tede/798 |
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por |
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por |
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openAccess |
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Universidade Estadual de Feira de Santana |
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Mestrado Acadêmico em Ciências Farmacêuticas |
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UEFS |
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Brasil |
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DEPARTAMENTO DE SAÚDE |
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Universidade Estadual de Feira de Santana |
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