Micropropagação e conservação de Comanthera mucugensis Giul. subsp. mucugensis
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UEFS |
Texto Completo: | http://localhost:8080/tede/handle/tede/589 |
Resumo: | Comanthera mucugensis Giul. subsp. mucugensis, an endemic species of the municipality of Mucugê-BA is threatened with extinction. To reduce extractivism in natural populations, it is necessary to develop efficient multiplication protocols; In this sense somatic embryogenesis and organogenesis may be viable and preservation cryopreservation may be a strategy for its long-term. The objectives of this study were: (1) to analyze the embryogenic competence and the indirect organogenesis of C. mucugensis; (2) to evaluate the cryopreservation of seeds and different methods of cryopreservation of C. mucugensis plants and (3) to identify the best way to store seeds in the long term. Experiments were performed to induce somatic embryogenesis with different concentrations of 2,4-D X BAP and Picloran X BAP and for indirect organogenesis BAP and ANA were used. Seeds were cryopreserved for 0 (control), 1, 7, 30, 360 and 540 days and other seeds were stored at different temperatures to verify the best form of storage. Plants were cryopreserved with the technique of vitrification and encapsulation-desiccation. Plants from cryopreserved seeds were acclimatized in sand and vegetal soil (1: 1) for 60 days. The work showed that the plant regulators Picloran and 2,4-D are promising in the induction of callus with embryogenic potential and that the plant regulator ANA at 4.9 μM is efficient in indirect organogenesis. The seeds of C. mucugensis can be cryopreserved without compromising their physiological quality, but the techniques for cryopreservation of plants have not been efficient. |
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Santana, José Raniere Ferreira dehttp://lattes.cnpq.br/837165033057126180557635500http://lattes.cnpq.br/4049049994581697Gurgel, Zafira Evelma da Rocha2018-01-30T22:51:06Z2017-07-24GURGEL, Zafira Evelma da Rocha. Micropropagação e conservação de Comanthera mucugensis Giul. subsp. Mucugensis. 2017. 93 f. Tese (Doutorado Acadêmico em Biotecnologia) - Universidade Estadual de Feira de Santana – Feira de Santana, 2017.http://localhost:8080/tede/handle/tede/589Comanthera mucugensis Giul. subsp. mucugensis, an endemic species of the municipality of Mucugê-BA is threatened with extinction. To reduce extractivism in natural populations, it is necessary to develop efficient multiplication protocols; In this sense somatic embryogenesis and organogenesis may be viable and preservation cryopreservation may be a strategy for its long-term. The objectives of this study were: (1) to analyze the embryogenic competence and the indirect organogenesis of C. mucugensis; (2) to evaluate the cryopreservation of seeds and different methods of cryopreservation of C. mucugensis plants and (3) to identify the best way to store seeds in the long term. Experiments were performed to induce somatic embryogenesis with different concentrations of 2,4-D X BAP and Picloran X BAP and for indirect organogenesis BAP and ANA were used. Seeds were cryopreserved for 0 (control), 1, 7, 30, 360 and 540 days and other seeds were stored at different temperatures to verify the best form of storage. Plants were cryopreserved with the technique of vitrification and encapsulation-desiccation. Plants from cryopreserved seeds were acclimatized in sand and vegetal soil (1: 1) for 60 days. The work showed that the plant regulators Picloran and 2,4-D are promising in the induction of callus with embryogenic potential and that the plant regulator ANA at 4.9 μM is efficient in indirect organogenesis. The seeds of C. mucugensis can be cryopreserved without compromising their physiological quality, but the techniques for cryopreservation of plants have not been efficient.Comanthera mucugensis Giul. subsp. mucugensis, possui flor de interesse comercial que devido ao extrativismo excessivo encontra-se ameaçada de extinção. Para suprir a demanda do mercado e evitar o declínio populacional, faz-se necessário desenvolver protocolos para sua multiplicação, podendo a embriogênese somática e a organogênese serem alternativas viáveis. Além disso, é importante investir em estudos de conservação a longo prazo como, por exemplo, a criopreservação. O trabalho teve como objetivos: realizar estudos para tornar a micropropagação mais eficiente e avaliar a conservação de C. mucugensis em diferentes temperaturas como estratégia para a conservação do seu germoplasma. Foram testados para a indução da embriogênese somática 2,4-D x BAP e Picloram x BAP e para organogênese indireta BAP e ANA. Na criopreservação foram avaliadas sementes mantidas em nitrogênio líquido (-196ºC) por 0, 1, 7, 30, 360 e 540 dias e as plantas inteiras foram submetidas a duas técnicas: vitrificação e encapsulamento-desidratação. Para avaliar o armazenamento, as sementes foram mantidas em temperatura ambiente, 4ºC, -20ºC, -80ºC e -196°C por 30, 90 e 180 dias. Visando observar se a criopreservação das sementes interfere no desenvolvimento, plantas oriundas da germinação in vitro de sementes criopreservadas foram aclimatizadas em areia e terra vegetal (1:1) por 60 dias. O trabalho demonstrou que os reguladores vegetais Picloram e 2,4-D são promissores na indução de calos com potencial embriogênico e que o regulador vegetal ANA (4,9 µM) é eficiente na organogênese indireta. As sementes de C. mucugensis podem ser criopreservadas sem comprometer sua qualidade fisiológica, entretanto, não foram eficientes as técnicas para criopreservação de plantas inteiras.Submitted by Jadson Francisco de Jesus SILVA (jadson@uefs.br) on 2018-01-30T22:51:06Z No. of bitstreams: 1 Tese Definitivo ZERGurgel.pdf: 2392487 bytes, checksum: 97870ae444449d037535f874c18b1d37 (MD5)Made available in DSpace on 2018-01-30T22:51:06Z (GMT). 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dc.title.por.fl_str_mv |
Micropropagação e conservação de Comanthera mucugensis Giul. subsp. mucugensis |
title |
Micropropagação e conservação de Comanthera mucugensis Giul. subsp. mucugensis |
spellingShingle |
Micropropagação e conservação de Comanthera mucugensis Giul. subsp. mucugensis Gurgel, Zafira Evelma da Rocha Sempre-viva Embriogênese somática Organogênese indireta Criopreservação Somatic Embryogenesis Indirect Organogenesis In Vitro Conservation CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
title_short |
Micropropagação e conservação de Comanthera mucugensis Giul. subsp. mucugensis |
title_full |
Micropropagação e conservação de Comanthera mucugensis Giul. subsp. mucugensis |
title_fullStr |
Micropropagação e conservação de Comanthera mucugensis Giul. subsp. mucugensis |
title_full_unstemmed |
Micropropagação e conservação de Comanthera mucugensis Giul. subsp. mucugensis |
title_sort |
Micropropagação e conservação de Comanthera mucugensis Giul. subsp. mucugensis |
author |
Gurgel, Zafira Evelma da Rocha |
author_facet |
Gurgel, Zafira Evelma da Rocha |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Santana, José Raniere Ferreira de |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/8371650330571261 |
dc.contributor.authorID.fl_str_mv |
80557635500 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/4049049994581697 |
dc.contributor.author.fl_str_mv |
Gurgel, Zafira Evelma da Rocha |
contributor_str_mv |
Santana, José Raniere Ferreira de |
dc.subject.por.fl_str_mv |
Sempre-viva Embriogênese somática Organogênese indireta Criopreservação |
topic |
Sempre-viva Embriogênese somática Organogênese indireta Criopreservação Somatic Embryogenesis Indirect Organogenesis In Vitro Conservation CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
dc.subject.eng.fl_str_mv |
Somatic Embryogenesis Indirect Organogenesis In Vitro Conservation |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
description |
Comanthera mucugensis Giul. subsp. mucugensis, an endemic species of the municipality of Mucugê-BA is threatened with extinction. To reduce extractivism in natural populations, it is necessary to develop efficient multiplication protocols; In this sense somatic embryogenesis and organogenesis may be viable and preservation cryopreservation may be a strategy for its long-term. The objectives of this study were: (1) to analyze the embryogenic competence and the indirect organogenesis of C. mucugensis; (2) to evaluate the cryopreservation of seeds and different methods of cryopreservation of C. mucugensis plants and (3) to identify the best way to store seeds in the long term. Experiments were performed to induce somatic embryogenesis with different concentrations of 2,4-D X BAP and Picloran X BAP and for indirect organogenesis BAP and ANA were used. Seeds were cryopreserved for 0 (control), 1, 7, 30, 360 and 540 days and other seeds were stored at different temperatures to verify the best form of storage. Plants were cryopreserved with the technique of vitrification and encapsulation-desiccation. Plants from cryopreserved seeds were acclimatized in sand and vegetal soil (1: 1) for 60 days. The work showed that the plant regulators Picloran and 2,4-D are promising in the induction of callus with embryogenic potential and that the plant regulator ANA at 4.9 μM is efficient in indirect organogenesis. The seeds of C. mucugensis can be cryopreserved without compromising their physiological quality, but the techniques for cryopreservation of plants have not been efficient. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017-07-24 |
dc.date.accessioned.fl_str_mv |
2018-01-30T22:51:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
GURGEL, Zafira Evelma da Rocha. Micropropagação e conservação de Comanthera mucugensis Giul. subsp. Mucugensis. 2017. 93 f. Tese (Doutorado Acadêmico em Biotecnologia) - Universidade Estadual de Feira de Santana – Feira de Santana, 2017. |
dc.identifier.uri.fl_str_mv |
http://localhost:8080/tede/handle/tede/589 |
identifier_str_mv |
GURGEL, Zafira Evelma da Rocha. Micropropagação e conservação de Comanthera mucugensis Giul. subsp. Mucugensis. 2017. 93 f. Tese (Doutorado Acadêmico em Biotecnologia) - Universidade Estadual de Feira de Santana – Feira de Santana, 2017. |
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http://localhost:8080/tede/handle/tede/589 |
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por |
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por |
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600 600 600 600 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Universidade Estadual de Feira de Santana |
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Doutorado Acadêmico em Biotecnologia |
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UEFS |
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Brasil |
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DEPARTAMENTO DE CIÊNCIAS BIOLÓGICAS |
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Universidade Estadual de Feira de Santana |
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