Fatores biótico e abióticos que influenciam na concentração de podofilotoxina
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UEFS |
Texto Completo: | http://localhost:8080/tede/handle/tede/234 |
Resumo: | This paper describes the influence of abiotic and biotic factors in the concentration of secondary metabolites of pharmaceutical interest in medicinal plants, while it aims to answer how these factors influence the concentration of podophyllotoxin and yateína Leptohyptis macrostachys in vitro, this goal. The specimen Leptohyptis macrostachys was established in vitro from seeds collected in the Chapada Diamantina-Ba; studying the rate of germination rate, the germination and mean germination of seeds grown on MS medium with different concentrations of salts constituents of this medium as well as different concentrations of sucrose and gibberellic acid. Was also studied, such as abiotic factors (culture medium, carbon source temperature, photoperiod and gas exchange) and biological (plant growth regulators) influenced the development (number of shoots and leaves and roots presence of friable callus, compact or oxidized) and production of podophyllotoxin and yateína. To study the influence of these factors in getting these metabolites, plants were steeped at 40 ° C in ethanol to produce the extract. This was partitioned with water and after a specific treatment, injected into the HPLC. We established a protocol for Leptohyptis macrostachys growing from seeds in ½ MS medium supplemented with 1.5% sucrose and 11.55 mM of GA3, there was no significant formation of callus in treatments applied and the concentration of podophyllotoxin was favored when L. macrostachys was grown in ½ MS medium supplemented with 1.5% sucrose, closed with PVC film and kept in a growth room at 30 ° C. |
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David, Juceni Phttp://lattes.cnpq.br/1499420381852420Meira, Paloma Ribeiro2015-10-14T21:06:54Z2013-09-19MEIRA, Paloma Ribeiro. Fatores biótico e abióticos que influenciam na concentração de podofilotoxina. 2013. 98f. Dissertação (Mestrado Acadêmico em Biotecnologia)- Universidade Estadual de Feira de Santana, Feira de Santana, 2013.http://localhost:8080/tede/handle/tede/234This paper describes the influence of abiotic and biotic factors in the concentration of secondary metabolites of pharmaceutical interest in medicinal plants, while it aims to answer how these factors influence the concentration of podophyllotoxin and yateína Leptohyptis macrostachys in vitro, this goal. The specimen Leptohyptis macrostachys was established in vitro from seeds collected in the Chapada Diamantina-Ba; studying the rate of germination rate, the germination and mean germination of seeds grown on MS medium with different concentrations of salts constituents of this medium as well as different concentrations of sucrose and gibberellic acid. Was also studied, such as abiotic factors (culture medium, carbon source temperature, photoperiod and gas exchange) and biological (plant growth regulators) influenced the development (number of shoots and leaves and roots presence of friable callus, compact or oxidized) and production of podophyllotoxin and yateína. To study the influence of these factors in getting these metabolites, plants were steeped at 40 ° C in ethanol to produce the extract. This was partitioned with water and after a specific treatment, injected into the HPLC. We established a protocol for Leptohyptis macrostachys growing from seeds in ½ MS medium supplemented with 1.5% sucrose and 11.55 mM of GA3, there was no significant formation of callus in treatments applied and the concentration of podophyllotoxin was favored when L. macrostachys was grown in ½ MS medium supplemented with 1.5% sucrose, closed with PVC film and kept in a growth room at 30 ° C.Este trabalho descreve a influência de fatores abióticos e biótico na concentração de metabólitos secundários de interesse farmacológico em plantas medicinais, ao mesmo tempo que se propõe a responder como esses fatores influenciam na concentração de podofilotoxina e yateína em Leptohyptis macrostachys in vitro, objetivo deste. A espécime Leptohyptis macrostachys foi estabelecida in vitro a partir de sementes coletadas na região da Chapada Diamantina-Ba; estudando o índice de velocidade de germinação, a germinabilidade e o tempo médio de germinação de sementes cultivadas em meio MS com diferentes concentrações de sais constituintes deste meio, bem como diferentes concentrações de sacarose e ácido giberélico. Foi estudado também, como fatores abióticos (meio de cultivo, fontes de carbono temperatura, fotoperíodo e trocas gasosas)e biótico (reguladores vegetais) influenciaram no desenvolvimento (quantidade de brotos folhas e raízes e presença de calos friáveis, compactos ou oxidados) e na produção de podofilotoxina e yateína. Para estudar a influência estes fatores na obtenção destes metabólitos, as plantas foram maceradas à 40ºC em etanol para a produção do extrato. Este foi particionado com água e, após tratamento específico, injetado no CLAE. Foi estabelecido um protocolo de cultivo para Leptohyptis macrostachys a partir de sementes em meio MS ½ suplementado com 1,5% de sacarose e 11,55µM de GA3; não houve formação significativa de calos nos tratamentos aplicados e a concentração da podofilotoxina foi favorecida quando L. macrostachys foi cultivada em meio MS ½ suplementado com 1,5% de sacarose, fechada com filme de PVC e mantida em sala de crescimento a 30ºC.Submitted by Verena Bastos (verena@uefs.br) on 2015-10-14T21:06:54Z No. of bitstreams: 1 Paloma_R._Meira-dissertação_2013.pdf: 1508066 bytes, checksum: cc4d4d39667159152b8407fa887ad2fa (MD5)Made available in DSpace on 2015-10-14T21:06:54Z (GMT). No. of bitstreams: 1 Paloma_R._Meira-dissertação_2013.pdf: 1508066 bytes, checksum: cc4d4d39667159152b8407fa887ad2fa (MD5) Previous issue date: 2013-09-19Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfporUniversidade Estadual de Feira de SantanaMestrado Acadêmico em BiotecnologiaUEFSBrasilDEPARTAMENTO DE CIÊNCIAS BIOLÓGICASpodofilotoxinaestabelecimento in vitromultiplicação in vitroLeptohyptisLamiaceaepodophylotoxinin vitro establishmentin vitro multiplicationLeptohyptis, LamiaceaeCIENCIAS BIOLOGICASFatores biótico e abióticos que influenciam na concentração de podofilotoxinainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis7701973706309601282600600600600-6971480722008537872-34391788430682021612075167498588264571info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UEFSinstname:Universidade Estadual de Feira de Santana (UEFS)instacron:UEFSORIGINALPaloma_R._Meira-dissertação_2013.pdfPaloma_R._Meira-dissertação_2013.pdfapplication/pdf1508066http://tede2.uefs.br:8080/bitstream/tede/234/2/Paloma_R._Meira-disserta%C3%A7%C3%A3o_2013.pdfcc4d4d39667159152b8407fa887ad2faMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://tede2.uefs.br:8080/bitstream/tede/234/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/2342015-10-14 18:06:54.669oai:tede2.uefs.br:8080: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.uefs.br:8080/PUBhttp://tede2.uefs.br:8080/oai/requestbcuefs@uefs.br|| bcref@uefs.br||bcuefs@uefs.bropendoar:2015-10-14T21:06:54Biblioteca Digital de Teses e Dissertações da UEFS - Universidade Estadual de Feira de Santana (UEFS)false |
dc.title.por.fl_str_mv |
Fatores biótico e abióticos que influenciam na concentração de podofilotoxina |
title |
Fatores biótico e abióticos que influenciam na concentração de podofilotoxina |
spellingShingle |
Fatores biótico e abióticos que influenciam na concentração de podofilotoxina Meira, Paloma Ribeiro podofilotoxina estabelecimento in vitro multiplicação in vitro Leptohyptis Lamiaceae podophylotoxin in vitro establishment in vitro multiplication Leptohyptis , Lamiaceae CIENCIAS BIOLOGICAS |
title_short |
Fatores biótico e abióticos que influenciam na concentração de podofilotoxina |
title_full |
Fatores biótico e abióticos que influenciam na concentração de podofilotoxina |
title_fullStr |
Fatores biótico e abióticos que influenciam na concentração de podofilotoxina |
title_full_unstemmed |
Fatores biótico e abióticos que influenciam na concentração de podofilotoxina |
title_sort |
Fatores biótico e abióticos que influenciam na concentração de podofilotoxina |
author |
Meira, Paloma Ribeiro |
author_facet |
Meira, Paloma Ribeiro |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
David, Juceni P |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/1499420381852420 |
dc.contributor.author.fl_str_mv |
Meira, Paloma Ribeiro |
contributor_str_mv |
David, Juceni P |
dc.subject.por.fl_str_mv |
podofilotoxina estabelecimento in vitro multiplicação in vitro Leptohyptis Lamiaceae |
topic |
podofilotoxina estabelecimento in vitro multiplicação in vitro Leptohyptis Lamiaceae podophylotoxin in vitro establishment in vitro multiplication Leptohyptis , Lamiaceae CIENCIAS BIOLOGICAS |
dc.subject.eng.fl_str_mv |
podophylotoxin in vitro establishment in vitro multiplication Leptohyptis , Lamiaceae |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS |
description |
This paper describes the influence of abiotic and biotic factors in the concentration of secondary metabolites of pharmaceutical interest in medicinal plants, while it aims to answer how these factors influence the concentration of podophyllotoxin and yateína Leptohyptis macrostachys in vitro, this goal. The specimen Leptohyptis macrostachys was established in vitro from seeds collected in the Chapada Diamantina-Ba; studying the rate of germination rate, the germination and mean germination of seeds grown on MS medium with different concentrations of salts constituents of this medium as well as different concentrations of sucrose and gibberellic acid. Was also studied, such as abiotic factors (culture medium, carbon source temperature, photoperiod and gas exchange) and biological (plant growth regulators) influenced the development (number of shoots and leaves and roots presence of friable callus, compact or oxidized) and production of podophyllotoxin and yateína. To study the influence of these factors in getting these metabolites, plants were steeped at 40 ° C in ethanol to produce the extract. This was partitioned with water and after a specific treatment, injected into the HPLC. We established a protocol for Leptohyptis macrostachys growing from seeds in ½ MS medium supplemented with 1.5% sucrose and 11.55 mM of GA3, there was no significant formation of callus in treatments applied and the concentration of podophyllotoxin was favored when L. macrostachys was grown in ½ MS medium supplemented with 1.5% sucrose, closed with PVC film and kept in a growth room at 30 ° C. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013-09-19 |
dc.date.accessioned.fl_str_mv |
2015-10-14T21:06:54Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MEIRA, Paloma Ribeiro. Fatores biótico e abióticos que influenciam na concentração de podofilotoxina. 2013. 98f. Dissertação (Mestrado Acadêmico em Biotecnologia)- Universidade Estadual de Feira de Santana, Feira de Santana, 2013. |
dc.identifier.uri.fl_str_mv |
http://localhost:8080/tede/handle/tede/234 |
identifier_str_mv |
MEIRA, Paloma Ribeiro. Fatores biótico e abióticos que influenciam na concentração de podofilotoxina. 2013. 98f. Dissertação (Mestrado Acadêmico em Biotecnologia)- Universidade Estadual de Feira de Santana, Feira de Santana, 2013. |
url |
http://localhost:8080/tede/handle/tede/234 |
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por |
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por |
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7701973706309601282 |
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600 600 600 600 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Universidade Estadual de Feira de Santana |
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Mestrado Acadêmico em Biotecnologia |
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UEFS |
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Brasil |
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DEPARTAMENTO DE CIÊNCIAS BIOLÓGICAS |
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Universidade Estadual de Feira de Santana |
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