Abiotic factors influencing podophyllotoxin and yatein overproduction in Leptohyptis macrostachys cultivated in vitro
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFBA |
Texto Completo: | http://repositorio.ufba.br/ri/handle/ri/26003 |
Resumo: | This work describes an in vitro propagation protocol for the large-scale cultivation of Leptohyptis macrostachys (Benth.) Harley & JFB Pastore and the influence of abiotic factors on podophyllotoxin and yatein production. The plant was established from seeds collected in Chapada Diamantina, BA, Brazil and submitted to different growth mediums and physical conditions. The podophyllotoxin and yatein contents were quantified by HPLC/DAD and with pure standards and these two lignans were present in all experiments. The lignan quantities were evaluated using the Sisvar Program, compared by Tukey’s test and hierarchical cluster analysis and principal component analysis. In all experiments, podophyllotoxin and yatein were detected at different concentrations. The best protocol cultivar of L. macrostachys was established from seeds in MS ½ medium supplemented with 1.5% sucrose and 11.55 μM of gibberellic acid A3 (GA3) at 30 °C, which yielded the highest concentration of podophyllotoxin (5.831 mg g−1). These results are important findings for the production of podophyllotoxin from the tissue culture. |
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Meira, Paloma R.David, Juceni P.Ribeiro, Erika M. de O.Santana, José R.F.Brandão, Hugo N.Oliveira, Lenaldo M. deDavid, Jorge M.Medrado, Héctor H.Pastore, José F.B.Meira, Paloma R.David, Juceni P.Ribeiro, Erika M. de O.Santana, José R.F.Brandão, Hugo N.Oliveira, Lenaldo M. deDavid, Jorge M.Medrado, Héctor H.Pastore, José F.B.2018-05-15T19:28:40Z2018-05-15T19:28:40Z2017http://repositorio.ufba.br/ri/handle/ri/26003v.22This work describes an in vitro propagation protocol for the large-scale cultivation of Leptohyptis macrostachys (Benth.) Harley & JFB Pastore and the influence of abiotic factors on podophyllotoxin and yatein production. The plant was established from seeds collected in Chapada Diamantina, BA, Brazil and submitted to different growth mediums and physical conditions. The podophyllotoxin and yatein contents were quantified by HPLC/DAD and with pure standards and these two lignans were present in all experiments. The lignan quantities were evaluated using the Sisvar Program, compared by Tukey’s test and hierarchical cluster analysis and principal component analysis. In all experiments, podophyllotoxin and yatein were detected at different concentrations. The best protocol cultivar of L. macrostachys was established from seeds in MS ½ medium supplemented with 1.5% sucrose and 11.55 μM of gibberellic acid A3 (GA3) at 30 °C, which yielded the highest concentration of podophyllotoxin (5.831 mg g−1). These results are important findings for the production of podophyllotoxin from the tissue culture.Submitted by Renorbio (renorbioba@ufba.br) on 2018-04-26T18:05:48Z No. of bitstreams: 1 Abiotic factors influencing podophyllotoxin and yatein overproduction in.pdf: 597838 bytes, checksum: fb8a604acb73230b893d24a0d35dd3bc (MD5)Approved for entry into archive by Edvaldo Souza (edvaldosouza@ufba.br) on 2018-05-15T19:28:40Z (GMT) No. of bitstreams: 1 Abiotic factors influencing podophyllotoxin and yatein overproduction in.pdf: 597838 bytes, checksum: fb8a604acb73230b893d24a0d35dd3bc (MD5)Made available in DSpace on 2018-05-15T19:28:40Z (GMT). No. of bitstreams: 1 Abiotic factors influencing podophyllotoxin and yatein overproduction in.pdf: 597838 bytes, checksum: fb8a604acb73230b893d24a0d35dd3bc (MD5) Previous issue date: 2017-11-05http://dx.doi.org/10.1016/j.phytol.2017.10.016reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAPodophyllotoxinIn vitro establishmentIn vitro multiplicationLeptohyptisAbiotic factors influencing podophyllotoxin and yatein overproduction in Leptohyptis macrostachys cultivated in vitroinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBrasilinfo:eu-repo/semantics/openAccessporORIGINALAbiotic factors influencing podophyllotoxin and yatein overproduction in.pdfAbiotic factors influencing podophyllotoxin and yatein overproduction in.pdfapplication/pdf597838https://repositorio.ufba.br/bitstream/ri/26003/1/Abiotic%20factors%20influencing%20podophyllotoxin%20and%20yatein%20overproduction%20in.pdffb8a604acb73230b893d24a0d35dd3bcMD51LICENSElicense.txtlicense.txttext/plain1383https://repositorio.ufba.br/bitstream/ri/26003/2/license.txt05eca2f01d0b3307819d0369dab18a34MD52TEXTAbiotic factors influencing podophyllotoxin and yatein overproduction in.pdf.txtAbiotic factors influencing podophyllotoxin and yatein overproduction in.pdf.txtExtracted texttext/plain28747https://repositorio.ufba.br/bitstream/ri/26003/3/Abiotic%20factors%20influencing%20podophyllotoxin%20and%20yatein%20overproduction%20in.pdf.txta98d00df0e9874806b03a47745bbae50MD53ri/260032022-10-21 19:37:47.15oai:repositorio.ufba.br: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ório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-10-21T22:37:47Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false |
dc.title.pt_BR.fl_str_mv |
Abiotic factors influencing podophyllotoxin and yatein overproduction in Leptohyptis macrostachys cultivated in vitro |
title |
Abiotic factors influencing podophyllotoxin and yatein overproduction in Leptohyptis macrostachys cultivated in vitro |
spellingShingle |
Abiotic factors influencing podophyllotoxin and yatein overproduction in Leptohyptis macrostachys cultivated in vitro Meira, Paloma R. Podophyllotoxin In vitro establishment In vitro multiplication Leptohyptis |
title_short |
Abiotic factors influencing podophyllotoxin and yatein overproduction in Leptohyptis macrostachys cultivated in vitro |
title_full |
Abiotic factors influencing podophyllotoxin and yatein overproduction in Leptohyptis macrostachys cultivated in vitro |
title_fullStr |
Abiotic factors influencing podophyllotoxin and yatein overproduction in Leptohyptis macrostachys cultivated in vitro |
title_full_unstemmed |
Abiotic factors influencing podophyllotoxin and yatein overproduction in Leptohyptis macrostachys cultivated in vitro |
title_sort |
Abiotic factors influencing podophyllotoxin and yatein overproduction in Leptohyptis macrostachys cultivated in vitro |
author |
Meira, Paloma R. |
author_facet |
Meira, Paloma R. David, Juceni P. Ribeiro, Erika M. de O. Santana, José R.F. Brandão, Hugo N. Oliveira, Lenaldo M. de David, Jorge M. Medrado, Héctor H. Pastore, José F.B. |
author_role |
author |
author2 |
David, Juceni P. Ribeiro, Erika M. de O. Santana, José R.F. Brandão, Hugo N. Oliveira, Lenaldo M. de David, Jorge M. Medrado, Héctor H. Pastore, José F.B. |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Meira, Paloma R. David, Juceni P. Ribeiro, Erika M. de O. Santana, José R.F. Brandão, Hugo N. Oliveira, Lenaldo M. de David, Jorge M. Medrado, Héctor H. Pastore, José F.B. Meira, Paloma R. David, Juceni P. Ribeiro, Erika M. de O. Santana, José R.F. Brandão, Hugo N. Oliveira, Lenaldo M. de David, Jorge M. Medrado, Héctor H. Pastore, José F.B. |
dc.subject.por.fl_str_mv |
Podophyllotoxin In vitro establishment In vitro multiplication Leptohyptis |
topic |
Podophyllotoxin In vitro establishment In vitro multiplication Leptohyptis |
description |
This work describes an in vitro propagation protocol for the large-scale cultivation of Leptohyptis macrostachys (Benth.) Harley & JFB Pastore and the influence of abiotic factors on podophyllotoxin and yatein production. The plant was established from seeds collected in Chapada Diamantina, BA, Brazil and submitted to different growth mediums and physical conditions. The podophyllotoxin and yatein contents were quantified by HPLC/DAD and with pure standards and these two lignans were present in all experiments. The lignan quantities were evaluated using the Sisvar Program, compared by Tukey’s test and hierarchical cluster analysis and principal component analysis. In all experiments, podophyllotoxin and yatein were detected at different concentrations. The best protocol cultivar of L. macrostachys was established from seeds in MS ½ medium supplemented with 1.5% sucrose and 11.55 μM of gibberellic acid A3 (GA3) at 30 °C, which yielded the highest concentration of podophyllotoxin (5.831 mg g−1). These results are important findings for the production of podophyllotoxin from the tissue culture. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
dc.date.accessioned.fl_str_mv |
2018-05-15T19:28:40Z |
dc.date.available.fl_str_mv |
2018-05-15T19:28:40Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufba.br/ri/handle/ri/26003 |
dc.identifier.number.pt_BR.fl_str_mv |
v.22 |
url |
http://repositorio.ufba.br/ri/handle/ri/26003 |
identifier_str_mv |
v.22 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.country.fl_str_mv |
Brasil |
dc.source.pt_BR.fl_str_mv |
http://dx.doi.org/10.1016/j.phytol.2017.10.016 |
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reponame:Repositório Institucional da UFBA instname:Universidade Federal da Bahia (UFBA) instacron:UFBA |
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Universidade Federal da Bahia (UFBA) |
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UFBA |
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