Tuberculosis in bovine slaughtered in the semi-arid region of Rio Grande do Norte, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/41613 |
Resumo: | Between November 2017 and August 2018, in order to determine the occurrence of tuberculosis in cattle slaughtered in the semi-arid region of Rio Grande do Norte, 11,610 bovine carcasses underwent a routine post-mortem inspection. One animal presented suggestive lesions and samples from lung, spleen and heart were submitted to microbiological culture in Stonebrink medium for 90 days and molecular analysis by nested-PCR. For histopathological examination and Ziehl Neelsen staining, the omentum was used. In the cultured samples, two of them (heart and spleen) showed growth, but were not confirmed as M. bovis by conventional PCR. At nested-PCR, the samples showed amplification for the TbD1 region. The presence of numerous granulomas was detected in the histopathological examination characterized by a necrotic center and areas of mineralization, as well as the presence of acid-fast bacilli (AFB) in the Ziehl-Neelsen stain. Microbiological culture can show false negative results, despite being considered a gold standard technique, although it takes time. Nested-PCR and histopathology show fast and effective results for the diagnosis of the disease. The presence of positive animals represents a public health risk in the studied region. Therefore, one of the essential systems applied to the control of bovine tuberculosis is the epidemiological surveillance of animals in slaughterhouses. |
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Tuberculosis in bovine slaughtered in the semi-arid region of Rio Grande do Norte, BrazilTuberculose em bovinos abatidos no semiárido do Rio Grande do Norte, BrasilMycobacterium bovisPublic healthSlaughterhousesTuberculosis.AbatedourosMycobacterium bovisSaúde públicaTuberculose.Between November 2017 and August 2018, in order to determine the occurrence of tuberculosis in cattle slaughtered in the semi-arid region of Rio Grande do Norte, 11,610 bovine carcasses underwent a routine post-mortem inspection. One animal presented suggestive lesions and samples from lung, spleen and heart were submitted to microbiological culture in Stonebrink medium for 90 days and molecular analysis by nested-PCR. For histopathological examination and Ziehl Neelsen staining, the omentum was used. In the cultured samples, two of them (heart and spleen) showed growth, but were not confirmed as M. bovis by conventional PCR. At nested-PCR, the samples showed amplification for the TbD1 region. The presence of numerous granulomas was detected in the histopathological examination characterized by a necrotic center and areas of mineralization, as well as the presence of acid-fast bacilli (AFB) in the Ziehl-Neelsen stain. Microbiological culture can show false negative results, despite being considered a gold standard technique, although it takes time. Nested-PCR and histopathology show fast and effective results for the diagnosis of the disease. The presence of positive animals represents a public health risk in the studied region. Therefore, one of the essential systems applied to the control of bovine tuberculosis is the epidemiological surveillance of animals in slaughterhouses.Entre novembro de 2017 e agosto de 2018, com objetivo de determinar a ocorrência de tuberculose em bovinos abatidos no semiárido do Rio Grande do Norte, 11.610 carcaças de bovinos passaram por inspeção post-mortem de rotina. Um animal apresentou lesões sugestivas e amostras de pulmão, baço e coração foram submetidas ao cultivo microbiológico em meio Stonebrink por 90 dias e análise molecular pela nested-PCR. O omento foi utilizado para exame histopatológico e coloração de Ziehl Neelsen. Nas amostras cultivadas, duas (coração e baço) evidenciaram crescimento, mas M. bovis não foi confirmado na PCR convencional. Já na nested-PCR, as amostras exibiram amplificação para a região TbD1. Foi detectada a presença de inúmeros granulomas no exame histopatológico caracterizados por possuírem um centro necrótico e áreas de mineralização, bem como a presença de bacilos Álcool-ácido resistentes (BAAR) na coloração de Ziehl-Neelsen. A cultura microbiológica pode apresentar resultados falso-negativos, apesar de ser considerada uma técnica padrão-ouro, embora demorada. A nested-PCR e a histopatologia apresentam resultados rápidos e eficazes para o diagnóstico da enfermidade. A presença de animais positivos representa um risco a saúde pública na região estudada. Portanto, um dos sistemas essenciais aplicados ao controle da tuberculose bovina é a vigilância epidemiológica dos animais em abatedouros.UEL2021-07-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisa de CampoAvaliado por paresPesquisa de Campoapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/4161310.5433/1679-0359.2021v42n5p3047Semina: Ciências Agrárias; Vol. 42 No. 5 (2021); 3047-3056Semina: Ciências Agrárias; v. 42 n. 5 (2021); 3047-30561679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/41613/29932Copyright (c) 2021 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessBezerra Neto, Pirajá SaraivaAraújo, Flábio Ribeiro dePasquatti, Taynara NunesGarino Junior, FelícioMedeiros, Giovanni BritoFirmino, Millena de OliveiraMedeiros, Rosália Severo deHigino, Severino Silvano dos Santos2022-09-30T13:38:39Zoai:ojs.pkp.sfu.ca:article/41613Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-09-30T13:38:39Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Tuberculosis in bovine slaughtered in the semi-arid region of Rio Grande do Norte, Brazil Tuberculose em bovinos abatidos no semiárido do Rio Grande do Norte, Brasil |
title |
Tuberculosis in bovine slaughtered in the semi-arid region of Rio Grande do Norte, Brazil |
spellingShingle |
Tuberculosis in bovine slaughtered in the semi-arid region of Rio Grande do Norte, Brazil Bezerra Neto, Pirajá Saraiva Mycobacterium bovis Public health Slaughterhouses Tuberculosis. Abatedouros Mycobacterium bovis Saúde pública Tuberculose. |
title_short |
Tuberculosis in bovine slaughtered in the semi-arid region of Rio Grande do Norte, Brazil |
title_full |
Tuberculosis in bovine slaughtered in the semi-arid region of Rio Grande do Norte, Brazil |
title_fullStr |
Tuberculosis in bovine slaughtered in the semi-arid region of Rio Grande do Norte, Brazil |
title_full_unstemmed |
Tuberculosis in bovine slaughtered in the semi-arid region of Rio Grande do Norte, Brazil |
title_sort |
Tuberculosis in bovine slaughtered in the semi-arid region of Rio Grande do Norte, Brazil |
author |
Bezerra Neto, Pirajá Saraiva |
author_facet |
Bezerra Neto, Pirajá Saraiva Araújo, Flábio Ribeiro de Pasquatti, Taynara Nunes Garino Junior, Felício Medeiros, Giovanni Brito Firmino, Millena de Oliveira Medeiros, Rosália Severo de Higino, Severino Silvano dos Santos |
author_role |
author |
author2 |
Araújo, Flábio Ribeiro de Pasquatti, Taynara Nunes Garino Junior, Felício Medeiros, Giovanni Brito Firmino, Millena de Oliveira Medeiros, Rosália Severo de Higino, Severino Silvano dos Santos |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Bezerra Neto, Pirajá Saraiva Araújo, Flábio Ribeiro de Pasquatti, Taynara Nunes Garino Junior, Felício Medeiros, Giovanni Brito Firmino, Millena de Oliveira Medeiros, Rosália Severo de Higino, Severino Silvano dos Santos |
dc.subject.por.fl_str_mv |
Mycobacterium bovis Public health Slaughterhouses Tuberculosis. Abatedouros Mycobacterium bovis Saúde pública Tuberculose. |
topic |
Mycobacterium bovis Public health Slaughterhouses Tuberculosis. Abatedouros Mycobacterium bovis Saúde pública Tuberculose. |
description |
Between November 2017 and August 2018, in order to determine the occurrence of tuberculosis in cattle slaughtered in the semi-arid region of Rio Grande do Norte, 11,610 bovine carcasses underwent a routine post-mortem inspection. One animal presented suggestive lesions and samples from lung, spleen and heart were submitted to microbiological culture in Stonebrink medium for 90 days and molecular analysis by nested-PCR. For histopathological examination and Ziehl Neelsen staining, the omentum was used. In the cultured samples, two of them (heart and spleen) showed growth, but were not confirmed as M. bovis by conventional PCR. At nested-PCR, the samples showed amplification for the TbD1 region. The presence of numerous granulomas was detected in the histopathological examination characterized by a necrotic center and areas of mineralization, as well as the presence of acid-fast bacilli (AFB) in the Ziehl-Neelsen stain. Microbiological culture can show false negative results, despite being considered a gold standard technique, although it takes time. Nested-PCR and histopathology show fast and effective results for the diagnosis of the disease. The presence of positive animals represents a public health risk in the studied region. Therefore, one of the essential systems applied to the control of bovine tuberculosis is the epidemiological surveillance of animals in slaughterhouses. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-07-02 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Pesquisa de Campo Avaliado por pares Pesquisa de Campo |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/41613 10.5433/1679-0359.2021v42n5p3047 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/41613 |
identifier_str_mv |
10.5433/1679-0359.2021v42n5p3047 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/41613/29932 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2021 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2021 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 42 No. 5 (2021); 3047-3056 Semina: Ciências Agrárias; v. 42 n. 5 (2021); 3047-3056 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
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1799306084672864256 |