Comparison between tests for tuberculosis diagnosis in slaughtered bovines

Detalhes bibliográficos
Autor(a) principal: Silva, David Attuy Vey Da
Data de Publicação: 2018
Outros Autores: Siconelli, Márcio Junio Lima, Bürger, Karina Paes [UNESP], Keid, Lara Borges
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/1808-1657000652016
http://hdl.handle.net/11449/212030
Resumo: Our goal for this article is to compare several different diagnosis tests for bovine tuberculosis identification. We have performed bacterial isolation, histopathological characterization, acid-fast bacilli (AFB) identification and M. bovis DNA detection. Lesions suggestive of Tuberculosis were sampled from bovine lymph nodes during slaughtering of bovines at an abattoir that operates under federal inspection. The bacterial isolation was performed in solid culture mediums, the histopathological characterization was made by Hematoxylin-eosinstaining, and AFB identification by Ziehl-Neelsen staining. Bacterial DNA detection was performed by Polymerase Chain Reaction (PCR) using DNA from two different sources, directly collected from the tuberculosis-like lesions (PCR followed by nested PCR) and from isolated bacteria. We have concluded that the multi-step approach, including histopathological characterization, bacterial isolation and AFB identification, is strongly recommended to diagnose tuberculosis in bovines. Furthermore, PCR assays using specimens of lesions suggestive of tuberculosis are a faster and more promising way to diagnose the disease. However, it should not be used alone due to the low sensitivity shown in this study.
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spelling Comparison between tests for tuberculosis diagnosis in slaughtered bovinesComparação entre testes de diagnóstico para tuberculose em bovinos abatidosinfectious diseasesMycobacterium bovistuberculosiszoonosisdoenças infecciosasMycobacterium bovistuberculosezoonoseOur goal for this article is to compare several different diagnosis tests for bovine tuberculosis identification. We have performed bacterial isolation, histopathological characterization, acid-fast bacilli (AFB) identification and M. bovis DNA detection. Lesions suggestive of Tuberculosis were sampled from bovine lymph nodes during slaughtering of bovines at an abattoir that operates under federal inspection. The bacterial isolation was performed in solid culture mediums, the histopathological characterization was made by Hematoxylin-eosinstaining, and AFB identification by Ziehl-Neelsen staining. Bacterial DNA detection was performed by Polymerase Chain Reaction (PCR) using DNA from two different sources, directly collected from the tuberculosis-like lesions (PCR followed by nested PCR) and from isolated bacteria. We have concluded that the multi-step approach, including histopathological characterization, bacterial isolation and AFB identification, is strongly recommended to diagnose tuberculosis in bovines. Furthermore, PCR assays using specimens of lesions suggestive of tuberculosis are a faster and more promising way to diagnose the disease. However, it should not be used alone due to the low sensitivity shown in this study.O objetivo deste estudo foi a comparação entre diferentes testes de diagnóstico para tuberculose bovina. Foram realizados o isolamento bacteriano, a caracterização histopatológica, a identificação de bacilos álcool-ácido resistentes e a detecção do DNA de M. bovis pela reação em cadeia da polimerase, em bovinos adultos abatidos em matadouros frigoríficos sob o Serviço de Inspeção Federal, valendo-se de amostras de linfonodos com lesões macroscópicas sugestivas de tuberculose, identificadas e coletadas durante o abate. O isolamento bacteriano foi realizado pelo cultivo em meios de cultura sólidos; a caracterização histopatológica, pela coloração com hematoxilina-eosina; e a identificação de bacilos álcool-ácido resistentes foi feita pela coloração de Ziehl-Neelsen. A detecção de DNA foi realizada em amostra extraída das lesões sugestivas de tuberculose pela reação em cadeia da polimerase, seguida da nested reação em cadeia da polimerase e por meio das colônias isoladas para identificação do M. bovis, utilizando-se também da reação em cadeia da polimerase . Os resultados obtidos permitiram concluir que os testes histopatológicos, o isolamento bacteriano e a identificação de bacilos álcool-ácido resistentes são aconselháveis para o diagnóstico da tuberculose bovina. Além disso, ensaios de reação em cadeia da polimerase utilizando amostras de lesões sugestivas de tuberculose são um modo mais rápido e promissor para diagnosticar a enfermidade, no entanto não deve ser utilizado sozinho, em virtude da baixa sensibilidade apresentada neste estudo.Universidade de Sao PauloUniversidade de Sao Paulo, Campus de Ribeirão PretoUniversidade Estadual Paulista “Julio de Mesquita Filho”Universidade Estadual Paulista “Julio de Mesquita Filho”Instituto BiológicoUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Silva, David Attuy Vey DaSiconelli, Márcio Junio LimaBürger, Karina Paes [UNESP]Keid, Lara Borges2021-07-14T10:33:24Z2021-07-14T10:33:24Z2018-08-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article-application/pdfhttp://dx.doi.org/10.1590/1808-1657000652016Arquivos do Instituto Biológico. Instituto Biológico, v. 85, p. -, 2018.0020-36531808-1657http://hdl.handle.net/11449/21203010.1590/1808-1657000652016S1808-16572018000100205S1808-16572018000100205.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArquivos do Instituto Biológicoinfo:eu-repo/semantics/openAccess2023-12-29T06:16:23Zoai:repositorio.unesp.br:11449/212030Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:34:12.446584Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Comparison between tests for tuberculosis diagnosis in slaughtered bovines
Comparação entre testes de diagnóstico para tuberculose em bovinos abatidos
title Comparison between tests for tuberculosis diagnosis in slaughtered bovines
spellingShingle Comparison between tests for tuberculosis diagnosis in slaughtered bovines
Silva, David Attuy Vey Da
infectious diseases
Mycobacterium bovis
tuberculosis
zoonosis
doenças infecciosas
Mycobacterium bovis
tuberculose
zoonose
title_short Comparison between tests for tuberculosis diagnosis in slaughtered bovines
title_full Comparison between tests for tuberculosis diagnosis in slaughtered bovines
title_fullStr Comparison between tests for tuberculosis diagnosis in slaughtered bovines
title_full_unstemmed Comparison between tests for tuberculosis diagnosis in slaughtered bovines
title_sort Comparison between tests for tuberculosis diagnosis in slaughtered bovines
author Silva, David Attuy Vey Da
author_facet Silva, David Attuy Vey Da
Siconelli, Márcio Junio Lima
Bürger, Karina Paes [UNESP]
Keid, Lara Borges
author_role author
author2 Siconelli, Márcio Junio Lima
Bürger, Karina Paes [UNESP]
Keid, Lara Borges
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Silva, David Attuy Vey Da
Siconelli, Márcio Junio Lima
Bürger, Karina Paes [UNESP]
Keid, Lara Borges
dc.subject.por.fl_str_mv infectious diseases
Mycobacterium bovis
tuberculosis
zoonosis
doenças infecciosas
Mycobacterium bovis
tuberculose
zoonose
topic infectious diseases
Mycobacterium bovis
tuberculosis
zoonosis
doenças infecciosas
Mycobacterium bovis
tuberculose
zoonose
description Our goal for this article is to compare several different diagnosis tests for bovine tuberculosis identification. We have performed bacterial isolation, histopathological characterization, acid-fast bacilli (AFB) identification and M. bovis DNA detection. Lesions suggestive of Tuberculosis were sampled from bovine lymph nodes during slaughtering of bovines at an abattoir that operates under federal inspection. The bacterial isolation was performed in solid culture mediums, the histopathological characterization was made by Hematoxylin-eosinstaining, and AFB identification by Ziehl-Neelsen staining. Bacterial DNA detection was performed by Polymerase Chain Reaction (PCR) using DNA from two different sources, directly collected from the tuberculosis-like lesions (PCR followed by nested PCR) and from isolated bacteria. We have concluded that the multi-step approach, including histopathological characterization, bacterial isolation and AFB identification, is strongly recommended to diagnose tuberculosis in bovines. Furthermore, PCR assays using specimens of lesions suggestive of tuberculosis are a faster and more promising way to diagnose the disease. However, it should not be used alone due to the low sensitivity shown in this study.
publishDate 2018
dc.date.none.fl_str_mv 2018-08-02
2021-07-14T10:33:24Z
2021-07-14T10:33:24Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/1808-1657000652016
Arquivos do Instituto Biológico. Instituto Biológico, v. 85, p. -, 2018.
0020-3653
1808-1657
http://hdl.handle.net/11449/212030
10.1590/1808-1657000652016
S1808-16572018000100205
S1808-16572018000100205.pdf
url http://dx.doi.org/10.1590/1808-1657000652016
http://hdl.handle.net/11449/212030
identifier_str_mv Arquivos do Instituto Biológico. Instituto Biológico, v. 85, p. -, 2018.
0020-3653
1808-1657
10.1590/1808-1657000652016
S1808-16572018000100205
S1808-16572018000100205.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Arquivos do Instituto Biológico
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv -
application/pdf
dc.publisher.none.fl_str_mv Instituto Biológico
publisher.none.fl_str_mv Instituto Biológico
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129336906088448

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