Molecular characterization of Cryptosporidium in calves from rural settlements in the Northwest region of the state of São Paulo, Brazil
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2019 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Semina. Ciências Agrárias (Online) |
| DOI: | 10.5433/1679-0359.2019v40n1p491 |
| Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/33245 |
Resumo: | The study was conducted on 25 properties of the settlements São José I and Salvador, located in the municipalities of Brejo Alegre and Birigui, in the State of São Paulo, Brazil. A record of variables was elaborated and included data such as gender, breed and age of the animals. A total of 231 stool samples were collected from bovines aged one to six months, 128 being females and 103 males, 131 crossbred and 100 Holstein. Among the 231 samples, 17 (7.36%) were positive for Cryptosporidium spp. both by malachite green negative staining and by nested-PCR. Of the 17 positive samples, 14 were sequenced in agarose gel. These sequences were detected between 99% and 100% of genetic similarity for the following species. One sequence was similar to C. parvum (AB513880.1), one to C. bovis (MF074602.1), two to C. ryanae (KT922233.1), one to C. felis (KM977642.1) and nine were similar for C. andersoni reference MF350628. C. andersoni was found in animals aged 2–6 months, an age group which is different from those described by several authors. The presence of C. parvum indicates that the calves in the studied region should be considered a potential source for zoonotic transmission. For the first time to our knowledge, C. felis was identified in cattle in America. |
| id |
UEL-11_0c661d74bf2ff1a73a2dc15b9fac94ad |
|---|---|
| oai_identifier_str |
oai:ojs.pkp.sfu.ca:article/33245 |
| network_acronym_str |
UEL-11 |
| network_name_str |
Semina. Ciências Agrárias (Online) |
| spelling |
Molecular characterization of Cryptosporidium in calves from rural settlements in the Northwest region of the state of São Paulo, BrazilCaracterização molecular de Cryptosporidium em bezerros de assentamentos rurais da região Noroeste do estado de São Paulo, BrasilCalvesCryptosporidiosisMolecular characterizationNested-PCR.BezerrosCaracterização molecularCriptosporidioseNested-PCR.The study was conducted on 25 properties of the settlements São José I and Salvador, located in the municipalities of Brejo Alegre and Birigui, in the State of São Paulo, Brazil. A record of variables was elaborated and included data such as gender, breed and age of the animals. A total of 231 stool samples were collected from bovines aged one to six months, 128 being females and 103 males, 131 crossbred and 100 Holstein. Among the 231 samples, 17 (7.36%) were positive for Cryptosporidium spp. both by malachite green negative staining and by nested-PCR. Of the 17 positive samples, 14 were sequenced in agarose gel. These sequences were detected between 99% and 100% of genetic similarity for the following species. One sequence was similar to C. parvum (AB513880.1), one to C. bovis (MF074602.1), two to C. ryanae (KT922233.1), one to C. felis (KM977642.1) and nine were similar for C. andersoni reference MF350628. C. andersoni was found in animals aged 2–6 months, an age group which is different from those described by several authors. The presence of C. parvum indicates that the calves in the studied region should be considered a potential source for zoonotic transmission. For the first time to our knowledge, C. felis was identified in cattle in America.O estudo foi realizado num total de 25 propriedades localizadas nos assentamentos São José I e Salvador, situados nos municípios de Brejo Alegre e Birigui, no estado de São Paulo. Um registro de variáveis foi elaborado, incluindo dados como sexo, raça e idade dos animais. Foram colhidas 231 amostras de fezes de bovinos de um a seis meses de idade, sendo 128 fêmeas e 103 machos, 131 mestiços e 100 da raça Holandesa. Entre os 231 bovinos examinados, 17 (7,36%) foram positivos para Cryptosporidium spp. tanto pela coloração negativa de verde malaquita como pela nested-PCR. Das 17 amostras positivas, 14 apresentaram amplificação pela eletroforese em gel de agarose suficiente para fazer o sequenciamento de DNA. Essas sequências foram detectadas similaridade genética entre 99% e 100% com as seguintes espécies. Uma sequência foi semelhante com C. parvum (referência: AB513880.1), uma com C. bovis (MF074602.1), duas com C. ryanae (KT922233.1), uma com C. felis (KM977642.1) e nove foram semelhantes com C. andersoni (MF350628). O estudo caracteriza a presença do Cryptosporidium spp. em bovinos oriundos de propriedades produtoras de leite na região Noroeste do estado de São Paulo, sendo o C. andersoni a espécie mais prevalente nesses animais, principalmente em uma faixa etária diferente das descritas por diversos autores. A presença de C. parvum indica que os bezerros da região estudada devem ser considerados como uma fonte potencial de oocistos de espécies zoonóticas. Identificamos com ineditismo o C. felis em bovinos na América, o que corrobora outros estudos realizados na Polônia e Espanha e evidencia a presença de espécies de Cryptosporidium em fezes em hospedeiros não naturais.UEL2019-02-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisa de laboratório e de campoAvaliado por paresapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3324510.5433/1679-0359.2019v40n1p491Semina: Ciências Agrárias; Vol. 40 No. 1 (2019); 491-496Semina: Ciências Agrárias; v. 40 n. 1 (2019); 491-4961679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/33245/25081Copyright (c) 2019 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessMatos, Lucas Vinicius Shigaki deSilveira Neto, Luiz daOliveira, Bruno César MirandaMakatu, Miriam YumiPierucci, Julia CestariViol, Milena AraúzGomes, Jancarlo FerreiraOliveira, Gilson Pereira deWidmer, GiovanniBresciani, Katia Denise Saraiva2022-10-19T15:37:54Zoai:ojs.pkp.sfu.ca:article/33245Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-19T15:37:54Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
| dc.title.none.fl_str_mv |
Molecular characterization of Cryptosporidium in calves from rural settlements in the Northwest region of the state of São Paulo, Brazil Caracterização molecular de Cryptosporidium em bezerros de assentamentos rurais da região Noroeste do estado de São Paulo, Brasil |
| title |
Molecular characterization of Cryptosporidium in calves from rural settlements in the Northwest region of the state of São Paulo, Brazil |
| spellingShingle |
Molecular characterization of Cryptosporidium in calves from rural settlements in the Northwest region of the state of São Paulo, Brazil Molecular characterization of Cryptosporidium in calves from rural settlements in the Northwest region of the state of São Paulo, Brazil Matos, Lucas Vinicius Shigaki de Calves Cryptosporidiosis Molecular characterization Nested-PCR. Bezerros Caracterização molecular Criptosporidiose Nested-PCR. Matos, Lucas Vinicius Shigaki de Calves Cryptosporidiosis Molecular characterization Nested-PCR. Bezerros Caracterização molecular Criptosporidiose Nested-PCR. |
| title_short |
Molecular characterization of Cryptosporidium in calves from rural settlements in the Northwest region of the state of São Paulo, Brazil |
| title_full |
Molecular characterization of Cryptosporidium in calves from rural settlements in the Northwest region of the state of São Paulo, Brazil |
| title_fullStr |
Molecular characterization of Cryptosporidium in calves from rural settlements in the Northwest region of the state of São Paulo, Brazil Molecular characterization of Cryptosporidium in calves from rural settlements in the Northwest region of the state of São Paulo, Brazil |
| title_full_unstemmed |
Molecular characterization of Cryptosporidium in calves from rural settlements in the Northwest region of the state of São Paulo, Brazil Molecular characterization of Cryptosporidium in calves from rural settlements in the Northwest region of the state of São Paulo, Brazil |
| title_sort |
Molecular characterization of Cryptosporidium in calves from rural settlements in the Northwest region of the state of São Paulo, Brazil |
| author |
Matos, Lucas Vinicius Shigaki de |
| author_facet |
Matos, Lucas Vinicius Shigaki de Matos, Lucas Vinicius Shigaki de Silveira Neto, Luiz da Oliveira, Bruno César Miranda Makatu, Miriam Yumi Pierucci, Julia Cestari Viol, Milena Araúz Gomes, Jancarlo Ferreira Oliveira, Gilson Pereira de Widmer, Giovanni Bresciani, Katia Denise Saraiva Silveira Neto, Luiz da Oliveira, Bruno César Miranda Makatu, Miriam Yumi Pierucci, Julia Cestari Viol, Milena Araúz Gomes, Jancarlo Ferreira Oliveira, Gilson Pereira de Widmer, Giovanni Bresciani, Katia Denise Saraiva |
| author_role |
author |
| author2 |
Silveira Neto, Luiz da Oliveira, Bruno César Miranda Makatu, Miriam Yumi Pierucci, Julia Cestari Viol, Milena Araúz Gomes, Jancarlo Ferreira Oliveira, Gilson Pereira de Widmer, Giovanni Bresciani, Katia Denise Saraiva |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Matos, Lucas Vinicius Shigaki de Silveira Neto, Luiz da Oliveira, Bruno César Miranda Makatu, Miriam Yumi Pierucci, Julia Cestari Viol, Milena Araúz Gomes, Jancarlo Ferreira Oliveira, Gilson Pereira de Widmer, Giovanni Bresciani, Katia Denise Saraiva |
| dc.subject.por.fl_str_mv |
Calves Cryptosporidiosis Molecular characterization Nested-PCR. Bezerros Caracterização molecular Criptosporidiose Nested-PCR. |
| topic |
Calves Cryptosporidiosis Molecular characterization Nested-PCR. Bezerros Caracterização molecular Criptosporidiose Nested-PCR. |
| description |
The study was conducted on 25 properties of the settlements São José I and Salvador, located in the municipalities of Brejo Alegre and Birigui, in the State of São Paulo, Brazil. A record of variables was elaborated and included data such as gender, breed and age of the animals. A total of 231 stool samples were collected from bovines aged one to six months, 128 being females and 103 males, 131 crossbred and 100 Holstein. Among the 231 samples, 17 (7.36%) were positive for Cryptosporidium spp. both by malachite green negative staining and by nested-PCR. Of the 17 positive samples, 14 were sequenced in agarose gel. These sequences were detected between 99% and 100% of genetic similarity for the following species. One sequence was similar to C. parvum (AB513880.1), one to C. bovis (MF074602.1), two to C. ryanae (KT922233.1), one to C. felis (KM977642.1) and nine were similar for C. andersoni reference MF350628. C. andersoni was found in animals aged 2–6 months, an age group which is different from those described by several authors. The presence of C. parvum indicates that the calves in the studied region should be considered a potential source for zoonotic transmission. For the first time to our knowledge, C. felis was identified in cattle in America. |
| publishDate |
2019 |
| dc.date.none.fl_str_mv |
2019-02-15 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Pesquisa de laboratório e de campo Avaliado por pares |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/33245 10.5433/1679-0359.2019v40n1p491 |
| url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/33245 |
| identifier_str_mv |
10.5433/1679-0359.2019v40n1p491 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/33245/25081 |
| dc.rights.driver.fl_str_mv |
Copyright (c) 2019 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
Copyright (c) 2019 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
UEL |
| publisher.none.fl_str_mv |
UEL |
| dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 40 No. 1 (2019); 491-496 Semina: Ciências Agrárias; v. 40 n. 1 (2019); 491-496 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
| instname_str |
Universidade Estadual de Londrina (UEL) |
| instacron_str |
UEL |
| institution |
UEL |
| reponame_str |
Semina. Ciências Agrárias (Online) |
| collection |
Semina. Ciências Agrárias (Online) |
| repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
| repository.mail.fl_str_mv |
semina.agrarias@uel.br |
| _version_ |
1822182759340179456 |
| dc.identifier.doi.none.fl_str_mv |
10.5433/1679-0359.2019v40n1p491 |