Molecular characterisation of Cryptosporidium spp. in lambs in the south central region of the State of São Paulo

Detalhes bibliográficos
Autor(a) principal: Zucatto, A. S. [UNESP]
Data de Publicação: 2015
Outros Autores: Aquino, M. C. C. [UNESP], Inácio, S. V. [UNESP], Figueiredo, R. N. [UNESP], Pierucci, J. C. [UNESP], Perri, S. H. V. [UNESP], Meireles, M. V. [UNESP], Bresciani, Katia Denise Saraiva [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000200441&lng=en&nrm=iso&tlng=en
http://hdl.handle.net/11449/127409
Resumo: Considering the proximity of sheep farmers to animals that are possibly diseased or releasing fecal oocysts into the environment and the marked pathogenicity in lambs, the aim of this study was to determine the occurrence and to molecularly characterize the infection by Cryptosporidium spp. in lambs in the South Central region of the state of São Paulo, Brazil. A total of 193 fecal samples were collected from sheep of several breeds, males and females, aged up to one year. Polymerase chain reaction (nested-PCR) was used to amplify DNA fragments from the subunit 18S rRNA gene and indicated 15% positivity; sequencing of amplified fragments was possible for 19 samples. Analysis of the obtained sequences showed that the identified species were Cryptosporidium xiaoi for 15 samples, constituting thus the first molecular characterization study of this Cryptosporidium species in Brazil. Cryptosporidium ubiquitum was identified for three samples and Cryptosporidium meleagridis for one sample; the latter two are considered zoonotic species.
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spelling Molecular characterisation of Cryptosporidium spp. in lambs in the south central region of the State of São PauloCaracterização molecular de Cryptosporidium spp. em cordeiros na região centro sul do Estado de São PauloCryptosporidiosisSheepNested-PCRGenotypeCryptosporidioseOvinos|Nested-PCRGenótipoConsidering the proximity of sheep farmers to animals that are possibly diseased or releasing fecal oocysts into the environment and the marked pathogenicity in lambs, the aim of this study was to determine the occurrence and to molecularly characterize the infection by Cryptosporidium spp. in lambs in the South Central region of the state of São Paulo, Brazil. A total of 193 fecal samples were collected from sheep of several breeds, males and females, aged up to one year. Polymerase chain reaction (nested-PCR) was used to amplify DNA fragments from the subunit 18S rRNA gene and indicated 15% positivity; sequencing of amplified fragments was possible for 19 samples. Analysis of the obtained sequences showed that the identified species were Cryptosporidium xiaoi for 15 samples, constituting thus the first molecular characterization study of this Cryptosporidium species in Brazil. Cryptosporidium ubiquitum was identified for three samples and Cryptosporidium meleagridis for one sample; the latter two are considered zoonotic species.Devido à proximidade de criadores de ovinos com animais possivelmente doentes e/ou eliminando oocistos fecais no ambiente e pela acentuada patogenicidade em cordeiros o objetivo foi, determinar a ocorrência e caracterizar molecularmente a infecção por Cryptosporidium spp. em cordeiros na região Centro Sul do Estado de São Paulo, Brasil. Num total de 193 amostras de fezes foram coletadas de ovinos de diversas raças, machos e fêmeas, com idade de até um ano. Por meio da reação em cadeia da polimerase (nested PCR) para a amplificação de fragmentos de DNA a partir do gene da subunidade 18S do rRNA houve positividade de 15% e o sequenciamento dos fragmentos amplificados foi possível em 19 amostras. A análise das sequências obtidas mostraram que as espécies identificadas nesses animais foram Cryptosporidium xiaoi em 15 amostras, sendo o primeiro estudo de caracterização molecular desta espécie de Cryptosporidium no Brasil. Cryptosporidium ubiquitum em três amostras, e Cryptosporidium meleagridis em uma amostra, sendo estas duas últimas consideradas espécies zoonóticas.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Estadual PaulistaUniversidade Estadual PaulistaFAPESP: 2010/13946-1FAPESP: 2010/52542-3Universidade Federal de Minas Gerais, Escola de VeterináriaUniversidade Estadual Paulista (Unesp)Zucatto, A. S. [UNESP]Aquino, M. C. C. [UNESP]Inácio, S. V. [UNESP]Figueiredo, R. N. [UNESP]Pierucci, J. C. [UNESP]Perri, S. H. V. [UNESP]Meireles, M. V. [UNESP]Bresciani, Katia Denise Saraiva [UNESP]2015-08-26T19:21:45Z2015-08-26T19:21:45Z2015-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article441-446application/pdfhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000200441&lng=en&nrm=iso&tlng=enArquivo Brasileiro de Medicina Veterinária e Zootecnia, Escola de Veterinária, v. 67, n. 2, p. 441-446, 2015.0102-0935http://hdl.handle.net/11449/12740910.1590/1678-7067S0102-09352015000200441S0102-09352015000200441.pdf0000-0003-0063-5172SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArquivo Brasileiro de Medicina Veterinária e Zootecnia0.2860,248info:eu-repo/semantics/openAccess2024-01-10T06:30:07Zoai:repositorio.unesp.br:11449/127409Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:39:18.457950Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Molecular characterisation of Cryptosporidium spp. in lambs in the south central region of the State of São Paulo
Caracterização molecular de Cryptosporidium spp. em cordeiros na região centro sul do Estado de São Paulo
title Molecular characterisation of Cryptosporidium spp. in lambs in the south central region of the State of São Paulo
spellingShingle Molecular characterisation of Cryptosporidium spp. in lambs in the south central region of the State of São Paulo
Zucatto, A. S. [UNESP]
Cryptosporidiosis
Sheep
Nested-PCR
Genotype
Cryptosporidiose
Ovinos
|Nested-PCR
Genótipo
title_short Molecular characterisation of Cryptosporidium spp. in lambs in the south central region of the State of São Paulo
title_full Molecular characterisation of Cryptosporidium spp. in lambs in the south central region of the State of São Paulo
title_fullStr Molecular characterisation of Cryptosporidium spp. in lambs in the south central region of the State of São Paulo
title_full_unstemmed Molecular characterisation of Cryptosporidium spp. in lambs in the south central region of the State of São Paulo
title_sort Molecular characterisation of Cryptosporidium spp. in lambs in the south central region of the State of São Paulo
author Zucatto, A. S. [UNESP]
author_facet Zucatto, A. S. [UNESP]
Aquino, M. C. C. [UNESP]
Inácio, S. V. [UNESP]
Figueiredo, R. N. [UNESP]
Pierucci, J. C. [UNESP]
Perri, S. H. V. [UNESP]
Meireles, M. V. [UNESP]
Bresciani, Katia Denise Saraiva [UNESP]
author_role author
author2 Aquino, M. C. C. [UNESP]
Inácio, S. V. [UNESP]
Figueiredo, R. N. [UNESP]
Pierucci, J. C. [UNESP]
Perri, S. H. V. [UNESP]
Meireles, M. V. [UNESP]
Bresciani, Katia Denise Saraiva [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Zucatto, A. S. [UNESP]
Aquino, M. C. C. [UNESP]
Inácio, S. V. [UNESP]
Figueiredo, R. N. [UNESP]
Pierucci, J. C. [UNESP]
Perri, S. H. V. [UNESP]
Meireles, M. V. [UNESP]
Bresciani, Katia Denise Saraiva [UNESP]
dc.subject.por.fl_str_mv Cryptosporidiosis
Sheep
Nested-PCR
Genotype
Cryptosporidiose
Ovinos
|Nested-PCR
Genótipo
topic Cryptosporidiosis
Sheep
Nested-PCR
Genotype
Cryptosporidiose
Ovinos
|Nested-PCR
Genótipo
description Considering the proximity of sheep farmers to animals that are possibly diseased or releasing fecal oocysts into the environment and the marked pathogenicity in lambs, the aim of this study was to determine the occurrence and to molecularly characterize the infection by Cryptosporidium spp. in lambs in the South Central region of the state of São Paulo, Brazil. A total of 193 fecal samples were collected from sheep of several breeds, males and females, aged up to one year. Polymerase chain reaction (nested-PCR) was used to amplify DNA fragments from the subunit 18S rRNA gene and indicated 15% positivity; sequencing of amplified fragments was possible for 19 samples. Analysis of the obtained sequences showed that the identified species were Cryptosporidium xiaoi for 15 samples, constituting thus the first molecular characterization study of this Cryptosporidium species in Brazil. Cryptosporidium ubiquitum was identified for three samples and Cryptosporidium meleagridis for one sample; the latter two are considered zoonotic species.
publishDate 2015
dc.date.none.fl_str_mv 2015-08-26T19:21:45Z
2015-08-26T19:21:45Z
2015-04-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000200441&lng=en&nrm=iso&tlng=en
Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Escola de Veterinária, v. 67, n. 2, p. 441-446, 2015.
0102-0935
http://hdl.handle.net/11449/127409
10.1590/1678-7067
S0102-09352015000200441
S0102-09352015000200441.pdf
0000-0003-0063-5172
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000200441&lng=en&nrm=iso&tlng=en
http://hdl.handle.net/11449/127409
identifier_str_mv Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Escola de Veterinária, v. 67, n. 2, p. 441-446, 2015.
0102-0935
10.1590/1678-7067
S0102-09352015000200441
S0102-09352015000200441.pdf
0000-0003-0063-5172
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Arquivo Brasileiro de Medicina Veterinária e Zootecnia
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dc.format.none.fl_str_mv 441-446
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dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais, Escola de Veterinária
publisher.none.fl_str_mv Universidade Federal de Minas Gerais, Escola de Veterinária
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
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institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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